• Korean Journal of Animal Reproduction
• /
• v.16 no.4
• /
• pp.317-323
• /
• 1993

This study was carried out to study effects of the addition level of acetamide and non-permeable cryoprotectants(Ficoll, sucrose) in VS(20% glycerol＋10% ethyleneglycol) and equilibration time on the survival of vitrified mouse morulae. The results are summarized as follows: 1. When 10, 15 and 20% of acetamide were added to the new vitrification solution(20G 10E), FDA-scores of embryos were 4.4(control), 4.4(10%), 3.6(15, 20%), respectively. The addition of acetamide did not affect the survival of forzen-thawed morulae(P<0.05). 2. The survival rate betwen 5 min(3.5) and 10 min(4.6), 10 min(4.6) and 20 min(3.2) of equilibration in 10% sucrose, and 20 min(3.2) and 5 min(4.0), or 10 min(4.3) in 20% sucrose were significantly different(P<0.05). The highest survival(4.6) rate was obtained in mouse morulae equilibrated in VS(20G 10E) containing 10% sucrose for 10 minutes. 3. FDA-score of morulae frozen in the new vitrification solution containing 0, 10, 20 and 30% Ficoll was 4.5, 4.2, 4.4 and 4.6, respectively and had no significant effect among concentrations of Ficoll(P>0.05). The development rate after culture(24h) was 89%(20% Ficoll) and 93%(30% Ficoll), respectively.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.13 no.6
• /
• pp.3055-3073
• /
• 2019

In this paper, a new user identification method is presented using real environmental human-computer-interaction (HCI) behavior data to improve method usability. User behavior data in this paper are collected continuously without setting experimental scenes such as text length, action number, etc. To illustrate the characteristics of real environmental HCI data, probability density distribution and performance of keyboard and mouse data are analyzed through the random sampling method and Support Vector Machine(SVM) algorithm. Based on the analysis of HCI behavior data in a real environment, the Multiple Kernel Learning (MKL) method is first used for user HCI behavior identification due to the heterogeneity of keyboard and mouse data. All possible kernel methods are compared to determine the MKL algorithm's parameters to ensure the robustness of the algorithm. Data analysis results show that keyboard data have a narrower range of probability density distribution than mouse data. Keyboard data have better performance with a 1-min time window, while that of mouse data is achieved with a 10-min time window. Finally, experiments using the MKL algorithm with three global polynomial kernels and ten local Gaussian kernels achieve a user identification accuracy of 83.03% in a real environmental HCI dataset, which demonstrates that the proposed method achieves an encouraging performance.

• Mass Spectrometry Letters
• /
• v.13 no.1
• /
• pp.20-25
• /
• 2022

Fargesin, a tetrahydrofurofuranoid lignan isolated from Flos Magnoliae, shows anti-inflammatory, anti-oxidative, anti-allergic, and anti-hypertensive activities. To evaluate the pharmacokinetics of fargesin in mice, a sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using electrospray ionization and parallel reaction monitoring mode was developed and validated for the quantification of fargesin in mouse plasma. Protein precipitation of 6 µL mouse plasma with methanol was used as sample clean-up procedure. The standard curve was linear over the range of 0.2-500 ng/mL in mouse plasma with the lower limit of quantification level at 0.2 ng/mL. The intra- and inter-day coefficient variations and accuracies for fargesin at four quality control concentrations including were 3.6-11.3% and 90.0-106.6%, respectively. Intravenously injected fargesin disappeared rapidly from the plasma with high clearance values (53.2-55.5 mL/min/kg) at 1, 2, and 4 mg/kg doses. Absolute bioavailability of fargesin was 4.1-9.6% after oral administration of fargesin at doses of 1, 2, and 4 mg/kg to mice.

• Korean Journal of Animal Reproduction
• /
• v.8 no.2
• /
• pp.116-121
• /
• 1984

These experiments were carried out to examine the effect of rapid thawing (500$^{\circ}C$/min) on the survival of 8-cell mouse embryos cooled slowly (0.5-1$^{\circ}C$/min) to precooling temperatures between -10 and 070$^{\circ}C$ before direct transfer ofembryos to -196$^{\circ}C$, and to compare the survival of embroys thawed slowly (20$^{\circ}C$/min) and rapidly (500$^{\circ}C$/min) after in vitro culture. In addition, the sensitivity of 8-cell mouse embroys to the rate of addition and removal of cryoprotectant at room temperauture, and the effect of developing stages on the survival of embryos frozen-thawed slowly were investigated. The results obtained were summarized as follows: 1. Embryos were survived in rapid thawing (500$^{\circ}C$/min) only when slow cooling was terminated at relatively high subzero teperaure (-20 to -60$^{\circ}C$). The highest survival rate(77.0%) in in vitro culture of embryos thawed rapidly was obtaeined after transfer to -196$^{\circ}C$ from -40$^{\circ}C$. 2. For the survival of embryos in slow thawing (20$^{\circ}C$/min.), slow cooling to lower subzero temperature (-50$^{\circ}C$ and below) was required before transfer of embryos to -196$^{\circ}C$. These results indicate that embryos transferred to -196$^{\circ}C$ from high subzero temprature contain much interacellular ice to damage them during slow warming but to permit survival of embryos after rapid warming. 3. The Survival rate (72.7%) of 8-cell mouse embryos after rapid addition and removal of cryoprotectant, DMSO at room temperature was similar to that (83.9%) after slow addition and removal of cryoprotectant at same temperature. 4. The survival rates of 1-, 2-, 4- and 8-cell embryos frozen-thawed slowly were 26.7, 76.4, 70.0 and 83.9%, respectively.

• Journal of Korean Physical Therapy Science
• /
• v.19 no.1
• /
• pp.47-54
• /
• 2012

Purpose : This study is to know the effect on muscle activity when people use mouse aid or not in computer working environment. Method : 11 people of both sex in their twentieth were tested. As a independent variable, the condition without mouse aid and the condition with movable mouse aid were selected, and as a dependent variable, MVC(%) on four muscles and inconvenience degree(RPE scale) on three body part(wrist, forearm, shoulder) were measured. Result : Analyzing the research, MVC(%) of extensor muscle of finger had notably higher muscle activity than other muscles, and there wasn't notable difference in muscle activity before and after using mouse aid. In case of using mouse aid, we tested by giving RPE questionnaire which segmentalized mental fatigue degree into hand, forearm, and shoulder, and resultingly, there wasn't notable differences perspectively. However, people felt less tiredness subjectively after they use the mouse aid. Conclusion : The usage of mouse aid seems to give comfort to computer users.

• Journal of the HCI Society of Korea
• /
• v.5 no.2
• /
• pp.1-6
• /
• 2010

In this research, we designed the head mouse system for disabled and gamers, a mouse controller which can be controlled by head movements and eye blinks only, and compared its performance with other regular mouse controller systems. The head mouse was moved by a gyro-sensor, which can measure an angular rotation of a head movement, and the eye blink was used as a clicking event of the mouse system. Accumulated errors caused by integral, which was a problem that previous head mouse system had, were removed periodically, and treated as dead zones in the non-linear relative point graph, and direct mouse point control was possible using its moving distance and acceleration calculation. We used the active light sources to minimize the influence of the ambient light changes, so that the head mouse was not affected by the change in external light source. In a comparison between the head mouse and the gazing tracking mouse (Quick Glance), the above method resulted about 21% higher score on the clicking event experiment called "20 clicks", about 25% higher on the dasher experiment, and about 37% higher on on-screen keyboard test respectively, which concludes that the proposed head mouse has better performance than the standard mouse system.

• Korean Journal of Oriental Medicine
• /
• v.9 no.1
• /
• pp.145-156
• /
• 2003

Obesity is caused by imbalance of energy intake and expense. If energy intake is more than its expenditure, body does fat accumulation and affects body weight. It can be fetal disease although obesity is not disease in itself. Central regulatary system is affected by many neurotransmitters regulating .food intake in brain. Hypothalamus was known as one of food intake regulation in CNS. In order to investigate gene expression difference in hypothalamus by different nutrient, we used C57/BL6 control mouse and db-/db- mouse. They divided each of two group with mouse, and fed control diet and high-fat diet for 4 weeks. Each of control and high-fat diet contained 11.7% and 59.7% fat, respectively. Then we performed microarray assay with them. We compared among changed genes in hypothalamus region. In the results, we observed that increased genes were more than decreased genes. Although hypothalamus size of db-/db- mouse is smaller than that of C57/BL6, more genes were affected in db-/db- mouse. In this study, many genes are affected by nutrient in hypothalamus region.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.7
• /
• pp.4135-4139
• /
• 2013

Inflammation is potential risk factor of various human malignancies. Inflammatory bowel syndromes such as ulcerative colitis are well known as risk factors for colon cancer. Here, we examined enhancing effects of dextran sulfate sodium (DSS)-associated inflammation on X-irradiation induced colonic tumorigenesis in Min and wild-type (WT) mice. Animals were X-irradiated at 1.5 Gy at 5 weeks of age (at 0 experimental week) and 2% DSS in drinking water was administered at 5 or 11 experimental weeks. Mice were sacrificed at 16 weeks and incidence and multiplicity of colonic tumors were assessed. Incidence of colonic tumors in Min mouse was increased from 33.3% to 100% (p<0.05) with X-irradiation alone, whereas no tumors were developed in WT mice. In DSS-treated Min mice, X-irradiation increased the number of colonic tumors. Total number of colonic tumors was increased 1.57 times to $30.7{\pm}3.83$ tumors/mouse with X-irradiation+DSS at 5 weeks comapared to $19.6{\pm}2.9$ in corresponding DSS alone group (p<0.05). When the duration of inflammation was compared, longer period of DSS effect promoted more colonic tumorigenesis. Collectively, we conclude that X-irradiation and DSS-induced inflammation act synergistically for colonic tumorigenesis.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.292.1-292.1
• /
• 2002

In order to understand the mechanism of action of TCDD. we have examined the effect of COX-inhibitors on cypla1 activity. We observed the effect of COX-inhibitor on EROD activity in C57BL/6 mouse in vovo. And we also evaluated the effect of COX-inhibitors on cypla1 mRNA. mouse cyplal promoter activity and EROD activity in Hepa cell. When Aspirin were pretreated with 3MC in vivo, the EROD activity that was stimulated by 3MC was inhibited. (omitted)

• Proceedings of the KSAR Conference
• /
• 2002.06a
• /
• pp.83-83
• /
• 2002

This study was to compare the characteristics of parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. Mouse oocytes were recovered from superovulated 4wks hybrid F1 (C57BL/6xCBA/N) female mice. The oocytes were treated with 7% ethanol for 5 min and 5 ㎍/㎖ cytochalasin-B for 4 h. For IVF, the oocytes were inseminated with epididymal sperm of hybrid Fl male mice (1×10/sup 6//㎖). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count in blastocysts was carried out differential labelling using propidium iodide (red) and bisbenzimide(blue). (omitted)