• 농업과학연구
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• 제38권3호
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• pp.459-464
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• 2011

A simplified model for the colloidal calcium phosphate (CCP) nanocluster was developed from an active role of phosphate in the precipitation of casein (CN)/Ca mixtures and the composition of casein micelles (CM). The possible shape of the CCP nanocluster was selected as a tetrahedron, and we estimated that 4 CN molecules were involved in crosslinking a single CCP nanocluster. Similar values were obtained for the number of CN molecules involved in stabilizing the nanocluster when the number of CNs attached onto each nanocluster surface was deduced from the composition of CM. If one phosphoserine cluster consisted of 3 phosphoserine residues, the theoretical molecular weight and volume for the nanocluster were estimated to be 4,898 g/mol and 2.88 $nm^3$, respectively. It was also shown that the position of Ca present in our model were reasonably located to accommodate the serine phosphate in CN molecule.

• 동아시아식생활학회지
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• 제13권1호
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• pp.9-18
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• 2003

This study was conducted to investigate the nutritional knowledge, nutrients intake and the assessment of dietary behavior of old peoples living in Incheon area. Two hundred and three elderly were examined using the questionnaire. The results of this study are summarized as follows. 1. The old people was lacking in correct information about nutrition and diseases. 2. The nutritional knowledge scores of old women were higher than those of old men and the scores went up according to the amount of pocket money. 3. The amount of vitamin C and phosphorus intakes were higher than those of the RDA, protein intake reached the level of the RDA. The vitamin B$_2$intake, however, was below 75% of the RDA and that of calcium was very low in women, especially. 4. Most of subjects had a meal regularly three times a day and the skipping rate of breakfast was very low. Dey had a moderate meal volume and did not have an unbalanced diet. Dey nearly do not take the processed food, dine out and exceed in the cholesterol intake. 5. They took green and yellow vegetables and, milk and milk products relatively small. On the other hand, the intake of caffein beverage was relatively high. the rates of drinking and smoking were low. From these results, we may propose the conclusion as follows: The old people were much concerned about the nutrition and diseases, but they had no correct informations about them. Therefore, the program of nutrition and dietary behavior for their healthy elderly life had to be prepared and to be applied to them immediately.

• Journal of Korean Neurosurgical Society
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• 제63권2호
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• pp.163-170
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• 2020

Objective : Milk fat globule-epidermal growth factor VIII (MFG-E8) may play a key role in inflammatory responses and has the potential to function as a neuroprotective agent for ameliorating brain injury in cerebral infarction. This study aimed to determine the role of MFG-E8 in brain injury in the subacute phase of cerebral ischemia in a rat model. Methods : Focal cerebral ischemia was induced in rats by occluding the middle cerebral artery with the modified intraluminal filament technique. Twenty-four hours after ischemia induction, rats were randomly assigned to two groups and treated with either recombinant human MFG-E8 or saline. Functional outcomes were assessed using the modified Neurological Severity Score (mNSS), and infarct volumes were evaluated using histology. Anti-inflammation, angiogenesis, and neurogenesis were assessed using immunohistochemistry with antibodies against ionized calcium-binding adapter molecule 1 (Iba-1), rat endothelial cell antigen-1 (RECA-1), and bromodeoxyuridine (BrdU)/doublecortin (DCX), respectively. Results : Our results showed that intravenous MFG-E8 treatment did not reduce the infarct volume; however, the mNSS test revealed that neurobehavioral deficits were significantly improved in the MFG-E8-treated group than in the vehicle group. Immunofluorescence staining revealed a significantly lower number of Iba-1-positive cells and higher number of RECA-1 in the periinfarcted brain region, and significantly higher numbers of BrdU- and DCX-positive cells in the subventricular zone in the MFG-E8-treated group than in the vehicle group. Conclusion : Our findings suggest that MFG-E8 improves neurological function by suppressing inflammation and enhancing angiogenesis and neuronal proliferation in the subacute phase of cerebral infarction.

• Journal of Nutrition and Health
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• 제20권1호
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• pp.25-37
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• 1987

The purpose of this study was to estimate the sodium and potassium intakes and their metabolisms of preschool children, and to evaluate the relationship between the blood pressure and the related variables. The subjects consisted of ninety-five preschool children aged two to six years (male 57, female 38). Twenty-four hour urines of subjects were collected for the measurements of their volume, sodium, potassium, creatinine and urea nitrogen. At the same time, the questionnaire was designed to assess the sodium and potassium intakes. The' results obtained were as follows; 1) The urinary excretion of sodium in 24 hours was 54.6$\pm$22.4mEq(orI255.8mg)and dietary sodium intake was 2147.0$\pm$518.4mg. The dietary sodium intake significantly increased with increasing age(p=O.0151). However, daily sodium intake per unit body surface area did not show significant difference by age. 2)The urinary excretion of potassium in 24 hours was 14.2$\pm$7.6 mEq (or 555.2mg) and the potassium intake was 1133.8mg. 3) The urinary excretions of creatinine and urea nitrogen were 240.2$\pm$126.2mg and 2946.7$\pm$1693.9mg, respectively. 4) The principal food SOUTce of sodium intake was the seasoning group, which con\ulcornertributed 49.9% to total sodium intake. 5) The main food source of potassium intake was milk and milk products; from which 28.6% of total potassium intake was obtained. 6) The blood pressure showed highly positive correlations with height, weight and body surface area (p$\leq$O.OOl) . In addition, the blood pressure was found to be correlat\ulcornered with urinary sodium excetion and dietary sodium intake (p$\leq$O.Ol).

• Journal of Pharmaceutical Investigation
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• 제26권3호
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• pp.221-232
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• 1996

An abrasive, calcium hydrogen phosphate dihydrate (DCPD), was synthesized in a Box-wilson experimental design by reactions between phosphoric acid and milk of lime, and calcium chloride and sodium phosphate solutions, and stabilized with TSPP and TMP. The optimum conditions for preparation of DCPD from phosphoric acid with milk of lime were such as; reaction temp.; $51.9^{\circ}C$, conc. of lime; 25.9%, conc. of phosphoric acd; 77.9%, drying temp.; $60.2^{\circ}C$ and final pH; 6.46. The physico-chemical and pharmaceutical properties of DCPD were showed as follows: glycerin absorption value(68 ml/100g), whiteness(99.5%), particle size(10.9 nm), pH(7.8), and set test(pass). XRD and SEM of DCPD indicated a monoclinic system crystallographically. $N_2$ adsorption isotherm curve by BET showed non porous type II form. The micromeritic parameters of DCPD showed that surface area was $3.27{\sim}4.6\;cm^{2}/g$ and pore volume, pore area and pore radius were negligible. The rheogram of the toothpaste containing DCPD showed pseudoplastic flow with yield value of 321, and thixotropic behavior forming hysteresis loop. These results meet the requirements as abrasive standard, and sythesized DCPD is expected as a good dental abrasive such as a high quality grade in practice.

• 한국식품조리과학회지
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• 제23권1호통권97호
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• pp.41-49
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• 2007

The substitution effects of whey protein isolate(WPI) for egg in the preparation of sponge cake were determined by objective and subjective tests. Milk whey is drained from milk curd as a by-product of cheese manufacture. Whey protein is known as a good nutritional source and a functional material for many processed foods, especially baked goods. WPI contains above 90% whey protein. The specific gravity and viscosity of sponge cakes tend to be affected by WPI substitution. The cooking loss of sponge cakes with WPI substituted for egg(abbreviated as WPI cake) during oven baking was smaller than that made with egg(abbreviated as egg cake) and the specific loaf volume of WPI cake was larger than that of egg cake. The number of pores was highly increased and the size of pores was more uniformly and finely distributed in the cross section of WPI cake than those of egg cake, as observed by scanning electron microscopy(SEM). The hardness, gumminess and chewiness of WPI cake made with 10-20% WPI substitution were the lowest among all the tested cakes, including egg cake, thereby confirming the considerable improvement in their cake qualities. By the results of sensory evaluation, appearance, pore uniformity, softness, chewiness, moistness, flavor, mouth feeling, and overall acceptability of 10-20% WPI substitute cakes were evaluated as being significantly superior to those of all other cakes(p<0.05). These results support the better physicochemical characteristics and sensory evaluations of sponge cake prepared with 10-20% of WPI substitution for egg.

• Journal of Periodontal and Implant Science
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• 제29권1호
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• pp.265-276
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• 1999

This study was performed to evaluate the effect of hydrogen peroxide-producing Lactobacillus acidophilus V-20onthe replication of periodontal pathogens, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. When A. actinomycetemcomitans and P. gingivalis were incubated alone and in the combination with L. acidophilus V-20, the viable cell numbers of A. actinomycetemcomitans and P. gingivalis were compared between those cultures. The effect of S. mutans, E. durans, and L. lactis on the replication of A. actinomycetemcomitans and P. gingivalis was also evaluated. The change of periodontal indexes(probine depth, gingival index, GCF volume) and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides in subgingival plaque sample were evaluated following gargling of fermented milk made from L. acidophilus V-20 for 1 month on patients with periodontal disease in maintenance phase. In the mixed culture of L. acidophilus V-20 and A. actinomycetemcomitans or P. gingivalis, the replication of A. actinomycetemcomitans or P. gingivalis wascompletely inhibited. But in the mixed culture of P. gingivalis and hydrogen peroxide-nonproducing Lactobacillus casei, the viable ceil numbers of P. gingivaliswas not decreased when compared with the numbers in the mixed culture of P. gingivalis and L. acidophilus V-20. In the mixed culture of A. actinomycetemcomitans and S. mutans, E. durans, or L. lactis, the viable cell number of A. actinomycetemcomitans was not almost changed when compared with the numbers in the culture of A. actinomycetemcomitans alone. And in the mixed culture of P. gingivalis and E. durans or L. lactis, the viable cell numbers of P. gingivaliswas not almost changed compared with the counts in the culture of P. gingivalis alone. But the replication of P. gingivalis was completely inhibited in the mixed culture of P. gingivalis and S. mutans. When the change of periodontal indexes following gargling of fermented milk was compared with baseline, probing depth and gingival index were not changed, but GCF volume was significantly decreased(p<0.05). And when the viable ceil numbers of microorganisms in subgingival plaque sample were compared with baseline, total viable ceil number was almost unchanged and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides were significantly decreased(p<0.05). These results suggest that L. acidophilus V-20 inhibit the replication of A. actinomycetemcomitans and black pigmented bacteroides by the formation of hydrogen peroxide.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1735-1741
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• 2004

발아현미를 이용한 죽의 관능적 특성을 바탕으로 최적 제조조건을 구하고자 중심합성 계획 법을 이용하여 실험을 하였으며, 반응표면분석 과 능선분석을 행하였다. 4요인중 발아현미죽의 관능특성에 가장 영향을 미치는 요인은 발아현미에 대한 물의 비율이었으며(p<0.01),다음은 우유의 비율로써 죽의 색상, 풍미 및 전체적인 기호도가 향상되었다(p<0.05). 실험결과 색상에 대한 최적조건은 발아현미에 대한 물의 비율이 7.76배, 조리시간이 56.53분, 조리온도가 96.87$^{\circ}C$,그리고 첨가되는 우유량의 비율이 0.50배이었다. 점성에 대한 최적조건은 발아현미에 대한 물의 비율이 8.62배, 조리시간이 39.07분, 조리온도가 95.93$^{\circ}C$, 그리고 첨가되는 우유량의 비율이 0.54배이었다. 풍미에 대한 최적조건은 발아현미에 대한 물의 비율이 8.49배, 조리시간이 35.55분, 조리온도가 95.81$^{\circ}C$, 그리고 첨가되는 우유량의 비율이 0.54배 이었다. 또한 입안에서의 질감에 대한 최적조건은 발아현미에 대한 물의 비율이 8.58배, 조리시간이 37.98분, 조리온도가 93.95$^{\circ}C$, 그리고 첨가되는 우유량의 비율이 0.55배이었고, 전반적 기호도에 대한 최적조건은 발아현미에 대한 물의 비율이 8.26배, 조리시간이 31.98분, 조리 온도가 95.71$^{\circ}C$, 그리고 첨가되는 우유량의 비율이 0.54배 등으로 각각 나타났다. 발아현미죽의 관능검사에서 전반적인 기호도는 점성, 향미, 입안에서의 질감 등이 기여도가 높고 색상의 영향은 적었다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.357-365
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• 2002

A survey was under taken of six district of Northern India viz. Bareilly, Pilibhit, Udham Singh Nagar, Nainital, Almora and Rampur. The age, breed, sex and physiological status recorded. A total number of 854 cattle examined out of which lactating (274 cases), non lactating (302 cases) heifers (128 cases), calves (82 cases) and adult male (68 cases) were examined. An incidence of 4.92 percent (42) of microfilarisis was recorded. The highest prevalence was observed in Rudrapur District of Udham Singh Nagar (33.33%, 4/12), followed by Lalkaun in Nanital District (21.74%, 10/46), Rampur (12.50%, 2/16), Bareilly (8.16%, 8/98) and Pilibhit (1.22%, 1/82). No infection was observed in Almora region. Amongst 854 cattle of different group incidence was highest in adult male (12.20%, 10/82), followed by non lactating (3.82%, 12/314) and lactating (2.70%, 2/74), (7.64%, 12/157) was found in Heifers. For haemeto-biochemical, serum minerals estimations and therapeutic study 32 animals suffering from filariasis and 18 healthy animals were taken. 16 animals were treated with ivermectin $@200{\mu}g/kg $ body weight. Effect of this disease on production has also been estimated for which body weight and milk production was observed. The main clinical manifestations observed were anaemia, loss of appetite, debility, oedematous swelling especially in the abdominal region, increased heart rate, and respiration rate. Haematological changes indicated decrease in hemoglobin, total erythrocyte count, packed cell volume, erthrocyte fragility and neutrophil, whereas there was significant increase in erythrocytes sedimentation rate (ESR), total leukocyte count (TLC), lymphocyte and eosinophils. Biochemical changes showed significant reduction in the values of serum albumin, A : G ratio, where as there was significant increase in blood glucose, blood urea nitrogen (BUN), globulin, total lipid, total cholesterol, phospholipids, serum bilirubin. Serum mineral profile also altered markedly, which indicate a significant decrease in Ca, Cu, Fe, Zn, and Mn with increase value of Na and Cl. There was no significant change in P and K values. Enzyme pattern in micro filaria infected animal indicated increased level of AST, ALT, alkaline phosphatase, ornithine carbamyl transferase, sorbitol dehydrogenase, glutamate dehydrogenase, isocitric dehydrogenase and lactate dehydrogenase. In blood gas values and acid/base balance, there was an increase in $PVCo_2$ and $PVo_2$. It has been observed that microfilaria infected cattle showed decrease in body weight and milk production. Animal treated with ivermectin showed the return of these above values toward normalcy.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1529-1534
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• 2004

The nutritive value of lentil straw (Lens culinaris) relative to 4% urea treated wheat straw (w/w) was assessed in adult bucks and milking buffaloes on-station and on-farm, respectively. A digestion-cum-nitrogen balance experiment was conducted with three bucks (24.35 kg) fed ad libitum lentil straw (LS), urea treated wheat straw (UTS) and a 1:1 (w/w DM basis) mixture of LS and UTS (LS:UTS) in a 3$\times$3 Latin square design. Though daily dry matter intake (g kg $W^{0.75}$ or % LW) of bucks fed LS and UTS did not differ significantly, that of LS:UTS was significantly (p<0.05) low. Similarly, digestibility of nutrients (DM, OM and CP) and nitrogen balance (g/day) did not differ significantly for bucks given LS or UTS although a significant positive improvement was evident in these parameters for LS:UTS. The digestibility of fibre fractions (NDF and ADF) was significantly (p<0.05) lower in LS as compared to UTS or LS:UTS. Except for the significantly (p<0.05) lower intake of DCP (g kg$W^{0.75}$), the bucks fed LS had statistically similar nutrient intake as compared to UTS. Feeding of LS:UTS resulted in significantly higher nutrient (DCP, TDN) intake by bucks. The important blood-biochemical parameters (haemoglobin,packed cell volume, glucose, urea nitrogen, total protein) and activity of serum enzymes (alanine aminotransferase and aspartate aminotransferase) did not differ significantly in goats irrespective of dietary treatment. In the onfarm study, eighteen multiparous milking buffaloes were randomly assigned into three equal groups to assess the relative effect of feeding untreated wheat straw (WS), UTS and LS:UTS ad libitum as basal feed with a supplement. The study continued for 3 months and revealed that daily dry matter intake and average milk production (liters/day) by buffaloes offered LS:UTS and UTS was statistically similar but it was significantly higher compare to WS. However, milk composition did not differ significantly among the dietary treatments. It may be concluded that the nutritive value of lentil straw appeared to be no different to UTS, however, a positive synergistic effect was evident by feeding a mixture of LS and UTS on performance of goats and lactating buffaloes.