• Journal of Korean society of Dental Hygiene
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• v.17 no.4
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• pp.657-667
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• 2017

Objectives: The aim of this study was to evaluate the potential of dental erosion by three different types of commercial fermented milk on the enamel surface of bovine teeth. Methods: Forty bovine teeth (four groups of 10) were immersed in fermented milk (experimental groups: liquid type, condense-stirred type and condense-drink type) or mineral water (control group) for 1, 3, 5, 10, 15, 30, 60, 90 and 120 minutes. Enamel surface microhardness was measured before and after treatment with a microhardness tester, and Scanning Electron Microscope (SEM) was used to assess the enamel surface morphology. Results:Changes in enamel surface microhardness (before-after treatment; ${\Delta}VHN$) were significantly different among the four groups (p<0.05). ${\Delta}VHN$ was highest in the liquid type group, followed by the condense-stirred type, condense-drink type, and control groups. The ${\Delta}VHNs$ of three types of fermented milk groups were higher than that of the control group (p<0.05). The liquid type group showed higher ${\Delta}VHN$ than the other two types of condense fermented milk groups (p<0.05). However, there was no significant difference in ${\Delta}VHN$ between the two types of condense fermented milk (p>0.05). The results of SEM observation have shown the most severe surface damage in bovine teeth immersed in the liquid type of fermented milk. Conclusions: In this study, it appears that liquid type fermented milk causes greater development of dental erosion. The physical properties and pH of fermented milk types must be considered for prevention of dental erosion associated with ingestion of fermented milk.

• Journal of the korean academy of Pediatric Dentistry
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• v.41 no.1
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• pp.1-7
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• 2014

If it is possible to preserve and culture cells from exfoliated deciduous teeth in a readily available storage medium within each family, more stem cells would be obtained. This research is about the effect of storage media and time on pulpal cell viability of exfoliated deciduous teeth. 330 exfoliated deciduous teeth were randomly divided into 11 groups; fresh group, dry group, groups stored in cell culture medium (2, 4, 7 days each), in milk (2, 4, 7 days each), and in saline (2, 4, 7 days each). Primary culture of pulpal cells was conducted in each group and the success rates were compared by calculating the number of teeth with viable cells. The result of primary culture shows that the success rate decreases as the time of storage gets longer. There was no statistical difference between groups stored in the cell culture medium, milk, and saline for 2 and 4 days. However, the groups stored in milk and saline for 7 days showed dramatic decrease in success rate compared to the group stored in the cell culture medium. In conclusion, exfoliated or extracted deciduous teeth can be used to culture pulpal cells when they are stored in milk and saline for a certain period of time; however obtaining viable pulpal cells becomes harder as the storage time gets longer.

• Journal of Korean society of Dental Hygiene
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• v.12 no.4
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• pp.733-739
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• 2012

Objectives : To Compare the degree of survival rate of gingival fibroblasts, which is concerned with teeth adherence based on the type of avulsed tooth's storage solution. Methods : Different media gingival fibroblasts were stored in Dulbecco's modified Eagle's medium(DMEM), Hank's balanced salt solution(HBSS), milk, saline, and green tea in for 1, 2, 3 hours. And, MTT assay was conducted to compare survival rate of human gingival fibroblasts. Results : 1. The survival rate of gingival fibroblasts in DMEM and HBSS was higher than thoes in other storage media( Milk> Saline> Green tea). 2. The survival rate of gingival fibroblasts in milk, saline and green tea decreased as time passed. 3. Because of low osmotic pressure, green tea showed decrease of survival rate of gingival fibroblasts. Conclusion : DMEM and HBSS were the most effective storage media for gingival fibroblast. Among milk, saline, green tea, milk is most effective storage media for keeping gingival fibroblasts. Milk is recommended for storage media of avulsed tooth for keeping viability of cells.

• Journal of Korean society of Dental Hygiene
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• v.4 no.1
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• pp.31-48
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• 2004

Dental caries taking place with milk teeth affects eruption of permanent teeth adversely, and thus, it is very important to prevent dental caries from taking place with a child in maintaining his/her physical, mental and emotional health. In this study as conducted to obtain basic data to be required for development of a program for letting preschool children form a habitual life for oral health and right dietary life, a total 172 children aged 5 years and 6 years were selected from children who lived in Hwasung-si, Kyunggi-do and attended preschools (childrens houses) located there with the consent of their guardians. Then, the weight length index (WLI) and blood components of each child were analyzed, and each relation between the weight length index (WLI) and dental caries of milk teeth and between blood components and dental caries of milk teeth was comparatively examined, and thereby, the following results were obtained. When the dmf rate of each group according to the obesity was examined, it was found to be 87.50% in the under-weight group, 71.20% in the normal weight group and 83.87% in the overweight group. And when the dmft rate of each group was examined, it was found to be 48.43% in the under-weight group, 37.10% in the normal weight group and 47.17% in the overweight group. Also, when the dmft index of each group was examined, it was found to be 3.9 in the under-weight group, 3.0 in the normal weight group and 3.8 in the overweight group. That is to say, the under-weight group and the overweight group having an abnormal weight were found to be higher in all of the dmf rate, the dmft rate and the dmft index than the normal weight group. It could be, therefore, known that normal weight should be maintained in order to prevent dental caries of milk teeth. And, it is thought that a right dietary life habit ensuring intake of balanced nutrients should be formed for maintenance of a normal weight. As a result of examining the relation between nutrient components in blood and dental caries of milk teeth, in the case of children with no dental caries, inorganic nutrient components of blood had a tendency to be a little higher, but no statistical significance was observed therein. Meanwhile, it was found that there was a relation between blood type and the dmft index.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.4
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• pp.419-428
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• 1997

The purpose of this study is to examine the effects of pre-school children's past lactation types and food habits on dental health status. This study was carried out by analyzing the information obtained from oral inspection of 163 preschool children and questionnaires answered by their mothers. The results are as follows: 1. 80.4% of the preschool children have decayed and filled teeth. 2. The older they are, the more decayed and filled teeth they have 3. The earlier they started to have weaning foods, the more decayed and filled teeth they have. Preschool children who started weaning foods after 12 months of their birth have significantly more decayed and filled teeth. 4. Preschool children who had weaning foods by using milk-bottles have significantly more decayed and filled teeth than others. 5. Preschool children who had weaning foods regularly have significantly less decayed and filled teeth. 6. Preschool children who have frequently skipped breakfast have the least decayed and filled teeth. 7. Preschool children who would like to have sweet things have significantly more decayed and filled teeth than others.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.2
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• pp.151-158
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• 2010

The purpose of this study was to investigate the tooth surface caries patterns in the primary dentition according to breast or bottle feeding. The subjects of study were 815 children, 36 to 71 months old, in Iksan, Cheongju and Ulsan cities. The caregivers of the children were asked which they fed between the breast milk and the infant formula during the 1st year after birth and the duration of feeding. There was no significant difference in dmfs between the breast milk group and the infant formula group(significance level 0.05, same below). Tooth groups, tooth surface groups, teeth, and tooth surfaces which had significantly higher dmfs in the breast milk group than in the infant formula group were upper anterior teeth(tooth groups), upper incisors' buccal and proximal surfaces(tooth surface groups), upper incisors and upper second molars(teeth), upper central incisors' buccal and distal surfaces, upper lateral incisors' buccolingual and proximal surfaces, upper 2nd molars'lingual, proximal, and occlusal surfaces, and lower 2nd molars' buccal and distal surfaces. In the breast milk group, tooth groups, tooth surface groups, teeth, and tooth surfaces which dmfs significantly increased as the duration of the feeding increased were upper anterior teeth and upper molars(tooth groups), upper anterior teeth's proximal surfaces, upper molars' buccolingual, proximal, and occlusal surfaces, lower molars' proximal surfaces(tooth surface groups), upper anterior teeth, upper molars, lower 2nd molars(teeth), upper anterior teeth's proximal surfaces, upper 1st molars'buccolingual, proximal, and occlusal surfaces, upper 2nd molars' buccal surfaces, and lower 2nd molars'mesial surfaces(tooth surfaces). In the infant formula group, dmfs increased after 3 years of feeding, but the difference was not significant. Caries prevention is necessary in case of breastfeeding more than two years.

• Journal of Korean society of Dental Hygiene
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• v.12 no.1
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• pp.27-35
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• 2012

Objectives : This study was performed to provide the basic data for oral health of infants, through the comparison of Streptococcus mutans activities(the representative bacteria of initial dental caries), in main nutritional foods for infants: breast milk, milk powder, and soya milk. Methods : The study targeted 30 infants and the saliva was sampled after the oral examination. The pH of sampled saliva was measured; and the sample of 'the saliva', 'the saliva and breast milk', and 'the saliva and formula milk' by researcher were produced; and the measurement was repeated 7 times. Results : As a result of examining the change of colony number of Streptococcus mutans by hour, the bacterial activity took in the source of nutrition took place in the first place; and in the group with dental caries, breast-feeding at night occurreds most frequently. Due to the combined activities, such as the increase in the contact time, the existence rate of dental caries appeared high. However, as the improvement of breast-feeding is closely related to the nutrition supply to babies, rather than the improvement of breast-feeding, the 12 hours after the culture in which Streptococcus mutans activities started was considered. Conclusions : In case of breast-feeding at night, it is necessary to remove the dental plaque in the mouth, before 12 hours has passed. Especially, using toothbrush and toothpaste to decrease the occurrence of dental caries, after the eruption of teeth, is recommended for oral administration.

• Journal of the Korean Dietetic Association
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• v.17 no.4
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• pp.364-377
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• 2011

This study was conducted to provide basic information on toothbrushing habits, snack intake frequency, and current dental caries in elementary school students. A total of 188 students (105 boys and 83 girls) in Changwon city participated in this study. Dental examination was conducted by two dental hygienists in July, 2010. The results were as follows. Exactly 86.2% of students were shown to have dental caries, which includes a sealant and removed teeth due to decay. The average number of teeth with dental caries per student was 3.8. Exactly 60.6% of students were shown to maintain correct toothbrushing habits to prevent dental caries, but only 12.8% brushed their teeth immediately after a meal or snack. Exactly 82.4% percent of participants received toothbrushing education, 47.1% of them received it from an expert, 43.9% received from their parents, and 28.2% used tooth paste with fluorine. Regarding snack intake frequency, ice cream was the most consumed cariogenic food and white milk was the most consumed protective food. The >=7 teeth with dental caries group consumed higher frequency of carbonated beverages intake than the 'no dental caries' group (P<0.05), and the '5~6 teeth with dental caries' group consumed higher frequency of chocolate intake than 'no dental caries' group (P<0.05).

• Journal of the korean academy of Pediatric Dentistry
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• v.47 no.3
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• pp.303-311
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• 2020

The aim of this study was to assess the awareness of caregivers, emergency medical technicians, and emergency medical doctors by investigating the storage media for avulsed teeth at the trauma site. This study was performed by reviewing medical records of 183 patients with the age under 17 years old, who visited Ajou University Hospital for dental trauma. Based on their medical records, storage media for avulsed permanent teeth were investigated by classifying them as dry, milk, saline, saliva and tap water. The storage media chosen by caregivers at the trauma site were classified as cases by parents and school nurse teachers. Storage media chosen by emergency medical technicians and emergency medical doctors were also investigated using the same classification criteria. The results show that both parents and school nurse teachers used milk the most as a storage medium for avulsed teeth. Dry storage was the second most chosen medium by parents, and saline by nurse teacher. As a storage media emergency medical technicians used saline the most, while emergency medical doctors used only saline when they failed to replant avulsed teeth. There is a need to improve the knowledge of caregivers, emergency medical technicians, and emergency medical doctors regarding the storage media for avulsed teeth.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.3
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• pp.358-366
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• 2009

Lactic acid bacteria worked positively on gastrointestinal tract and oral environment. So I selected commercial five fermented milks and milk, and then I evaluated their effect of growth inhibition and biofilm formation of cariogenic bacteria, Streptococcus mutans. And also calculated the acidity, buffering capacity, concentration of Ca and P ion and pH change of those drinks. After adding S. mutans to fermented milks viable cell count of S. mutans in milk was not statistically different but those in all fermented milks were decreased as concentration of fermented milk increased. When I measured the amount of formed biofilm in 10% fermented milks and milk with S. mutans and compared with those without S. mutans, the amount was decreased in Active GG and Bulgaris while being increased in Tootee, Ace and milk(P<0.05). The fermented milk with the lowest pH value was E5(3.48${\pm}$0.01), and the highest was Bulgaris(4.19${\pm}$0.02). pH change of the fermented milks and milk with S. mutans was measured. The highest acid producing fermented milk was Bulgaris, and followed by Active GG, Ace, Tootee, E5, Milk. These results indicated that fermented milks had caries activity due to the value of initial acidity and acid producing capacity. But, concentrated fermented milks had the inhibitory effect against S. mutans, and also had high volume of Ca and P ion that protected teeth. So I suggest that they have positive effect on teeth.