• 대한지역사회영양학회지
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• 제11권3호
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• pp.327-337
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• 2006

The major purpose of this study was to develop the nutrition education contents and material through the basic data from the service provider and the consumer. And also, to find out the differences of attitude and needs between the service provider (SP breastfeeding specialist), present consumer (PC, pregnant or lactating women) and future consumer (FC, college women). There were types of questionnaires, which consisted of needs and attitudes toward child and maternal nutrition, as well as the personal characteristics of the study subjects. The subjects consisted of 113 breastfeeding specialists who served at medical related institutions, 197 pregnant or lactating women and 309 college women. The self-administered questionnaires from subjects were collected from October to November 2005 in Seoul and Kyunggi Province. The data was analysed by SPSS Win 12.0, ANOVA and Kruskal-Wallis test. The results of this study were as following: 1) The most Important determining factors on breastfeeding was 'medical specialist's support' (3.51) and the next one was 'knowledge of maternity care practice' (3.39). And the importance score of determinating factors on breastfeeding was significantly different between each group (p < 0.001). The groups of consumer (3.50 at PC and 3.59 at FC) considered the service provider (3.32) more important. The service providers considered a more effective determining factor to be 'husband and family support' and 'peer support' than the others (p <0.001). 2) To compare the effective factors of breastfeeding practices between the three groups, the service providers were more significantly considered than the otters such as 'attendance of intervention program' (p < 0.001). But the PC group considered the most effective factors wis 'mother's job after delivery'. 3) The self-evaluated score of the breastfeeding knowledge was the most high in SP; the score was significantly different between groups. The knowledge score of nutritional aspects in human milk was most highly evaluated. But maternity care practice and public acceptance marked the lower evaluation score than other issues. 4) The desirable types of educational material was mass media, and the next was printed matter such as booklets. Two kinds if consumers preferred DVD or VCR tapes than and the service provider group (p < 0.001). 5) The priority contents of nutrition service PC group wanted the information about infant care more than maternal care (p < 0.05), but FC group's priority was significantly different compared with PC group (p < 0.001). The priority of SP group pointed out the information of practical child care methods. The results showed the needs of nutrition service, education channels, and perception toward effective factors on consumer behavior changes were significantly different between each group. Thus the result of this study may suggest that consumer oriented nutrition service programs must be developed.

• 한국식생활문화학회지
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• 제29권1호
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• pp.9-17
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• 2014

This study was conducted to develop a Han-sik database as well as to assess the effects of Han-sik on dietary quality among Koreans. The Han-sik database was developed by a focus group composed of food and nutrition specialists considering the results of Han-sik perception surveys conducted in previous studies for frequently consumed dishes. Among the 1,322 dish items identified in the $4^{th}$ (2007-2009) Korea National Health and Nutrition Examination Survey (KNHANES), 973 items (73.6%) were classified as Han-sik. Han-sik usage was defined as the percentage of Han-sik items of all individually consumed dish items in the dietary data of 22,113 subjects who participated in a 24-hour diet recall in the $4^{th}$ (2007-2009) KNHANES. Dietary quality was evaluated based on adherence to the Korean Food Guidance System (KFGS), which was calculated as the percentage of the number of servings consumed in each food group out of recommended servings according to age and sex. Mean usage of Han-sik was 80.1% and was higher in older age groups, rural areas, as well as in households at the lowest income level. Han-sik usage was also higher on weekdays as well as for breakfast. Adherence to the KFGS was significantly higher for grains, meat fish egg beans, vegetables, and fruits (p<0.001) but lower for milk dairy products and oils fats sugars (p<0.001) across the quartiles of Han-sik usage. The results of this study indicate that Han-sik usage is high among Koreans. Further studies are needed to update the Han-sik database as well as investigate the association between health-related factors and Han-sik use.

• 대한임상검사과학회지
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• 제47권3호
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• pp.105-111
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• 2015

2013년 12월 말, 에볼라 바이러스는 서아프리카에서 발발했다. 기니를 시작으로 라이베리아, 시에라리온으로 빠르게 퍼지게 되었다. 에볼라 바이러스(자이르형 에볼라 바이러스)는 외피, 비분절, 음성단일가닥 RNA바이러스이다. 에볼라 바이러스는 인수공통 전염병이다. 바이러스는 처음 감염된 야생동물과 접촉 한 후, 혈액, 땀, 소변, 정액, 모유 등의 체액과의 직접적인 접촉을 통해 사람 대 사람으로 전염된다. 그러나 공기로 전염되지는 않는다. 잠복기는 2~21일이다. 에볼라 바이러스는 내피세포, 단핵 식세포, 간세포를 감염시킨다. 감염 후 바이러스가 숙주의 면역 시스템을 회피하기 위해 여러 메커니즘을 사용한다. 이것은 혈관, 간 등의 내부 조직 및 기관에 막대한 피해를 주어 죽음에 이르게 한다. RNA 바이러스에 대한 대부분의 실험은 역전사 중합효소 연쇄반응(RT-PCR)라 기술에 의존한다. 이 방법은 매우 민감하지만 숙련된 과학자, 전원 공급 장치 요구하며 비싸다. 스트립 분석기법(효소면역분석법, ELISA)은 에볼라 바이러스 항원 또는 항체를 검출한다. 이 기법은 저렴하며, 전기, 냉장 장치가 필요하지 않다. 실험적인 치료 및 백신 개발에 관한 지속적인 노력에도 불구하고, 에볼라 바이러스 질환은 현재 치료법에 제한이 있다. 그러므로 신속하고 정확한 진단이 환자관리, 감염예방, 관리대책에 있어서 매우 중요하다.

• Journal of Dairy Science and Biotechnology
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• 제34권4호
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• pp.231-237
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• 2016

Kefir is a probiotic food. Probiotics have shown to be beneficial to health, and are currently of great interest to the food industry. Hence, this study was carried out to evaluate the lactase activity of kefir-isolated lactic acid bacteria. Three strains, Lactobacillu kefiri DH5 isolated from the kefir grains and Bifidobacterium animalis subsp. Lactis and Bifidobacteria longum 720, commercial probiotic LAB, were fermented in 10% reconstituted nonfat dry milk suspensions and incubated at $37^{\circ}C$ for 48 h, and then analyzed for various saccarides by HPLC. The results showed that changes in the concentrations of lactose and galactose were significantly decreased and increased, respectively (p<0.05), but all 3 probiotic strains tested in this study showed no increase in glucose concentration during 48 h of incubation. Both DH5 and BL720 showed high lactase activities (p<0.05), whereas BLC exhibited the lowest activity. Additionally, all three lactic acid bacteria showed high tagatose, but did not show high xylose and sedoheptulose. Finally, DH5, a kefir-isolated LAB, may have similar characteristics and properties to typical Bifidobacterium spp. and showed higher lactase activity than commercial Bifidobacterium spp.

• 대한영양사협회학술지
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• 제10권4호
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• pp.407-416
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• 2004

The purpose of this study was to examine the comparison of growth development, bone mineral density and nutrient intakes between double income families' preschool children(DIFPC) and full-time housewives' preschool children(FHPC). Subjects were 111 preschool children. Anthropometric characteristics and bone mineral density in right forearm were measured. The questionnaire was composed of health status, life style, dietary behaviors, and dietary intakes and was completed by the children's mothers. The average age of the DIFPC(n=60) was 53.02 months and that of the FHPC(n=51) was 54.80 months. The birth height and weight of the subjects were 50.47㎝ and 3.27㎏ for DIFPC and 50.85㎝ and 3.36㎏ for FHPC, respectively. The average height, weight, % body fat, and obesity index were 108.50㎝, 18.35㎏, 15.35%, 96.71% in DIFPC and 111.46㎝, 19.64㎏, 16.80%, 97.31% in FHPC, respectively. The bone mineral density in forearm of two groups were 0.24g/㎠ in all. The infant feeding method was significantly different between DIFPC and FHPC; 58.9% of DIFPC was fed formula, while 44.4% of FHPC was fed breast milk(p<0.05). Proportions of children for their regular meal were 59.4%, 89.6%, and 61.0% for breakfast, lunch, and dinner, respectively. The major reasons for irregular meal were lack of time and poor appetite for breakfast and snacks for lunch and dinner. Most of the children answered they have snack over once a day, and 60.0% have unbalanced diet. The intakes of energy, calcium, iron, zinc, vitamin A, vitamin B1, niacin, and vitamin C did not meet the Korean RDAs. The intakes of K and vitamin A for DIFPC were significantly higher than those of FHPC(p<0.05, p<0.05). In conclusion, double income families' preschool children more have a low frequency of breast feeding and low intakes of micro nutrients, such as K and vitamin A than full-time housewives' ones.

• 한국식품영양학회지
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• 제28권4호
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• pp.728-736
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• 2015

Cronobacter spp.는 Salmonella spp.와 함께 조제분유에서 발견되는 미생물 중 위험도가 가장 높은 category A에 속하는 균으로 알려져 있다. 1958년 영국에서 처음으로 유아의 뇌수 막염의 원인균으로 보고되었으며, 감염 후 신생아에게서 괴사성 장염, 패혈증 등을 일으켜 후유증으로 시력과 청력 상실 및 신경마비를 일으키기도 한다. 이러한 위험성 때문에 Cronobacter spp.의 신속 검출 및 진단은 식중독 예방에 있어서 중요하다. 따라서 2002년 미국 식품의약품안전국에서는 분유에서의 Cronobacter spp.를 EE broth, VRBG 배지를 이용해 균을 분리 하고, TSA 배지에 순수분리 후 노란색을 발하는 colony를 생화학적 실험을 통해 검출하는 방법을 제시했다. 또한, 우리나라 식품공전에서도 배지를 이용하여 Cronobacter spp.를 검출 하는 방법에 대해 등재하였다. 하지만 배지배양법을 기반으로 하는 Cronobacter spp.의 검출방법은 검출에 소요되는 시간과 노동력 등이 비효율적이라는 단점이 있다. 따라서 많은 시간이 소요되는 배지배양법을 보완하고자 PCR, real-time PCR 방법 및 최근에는 PCR기법과 ELISA, CE-LIF 등을 결합하여 검출하는 방법이 개발되어 Cronobacter spp.의 검출에 사용되기도 하였다. Cronobacter spp.의 검출에 사용되고 있는 분자생물학적 기반의 PCR 및 real-time PCR 기법은 민감도와 특이도가 좋으며, 배지배양법에 의한 검출방법에 비해 검출 시간을 획기적으로 줄일 수 있다는 장점이 있다. 하지만 까다로운 실험과정과 조작에 있어서 필요한 전문적인 기술이 필요함은 한계점으로 사료된다. 면역학적 방법에 의한 Cronobacter spp. 검출에 관한 연구를 통해, 분석에 소요되는 상당시간을 단축할 수 있었으며, 민감하고 특이적인 검출이 가능함을 보여주었다. 특히 일부 연구보고에서 면역학적 검출방법은 고가의 장비가 필요 없으며, 간단한 지침에 따라 모니터링 업무의 수행이 가능하다고 하였다. 면역학적 검출방법에는 ELISA 방법 외에도 immunomagnetic bead, liposome, immunochromatographic strip 등이 개발되고 있다. 우리나라 식품의약품안전처에서는 영 유아 대상 식품의 안전관리를 강화하고자 Cronobacter spp.에 대해서는 '불검출'로 기준을 설정 운영하고 있으며, 지속적으로 이에 관한 규정을 강화하고 있는 실정이다. 따라서, 식품산업체 및 식품 의 제조, 가공, 유통 현장에서 쉽게 모니터링이 가능하며, 신속, 민감하고 특이적인 검출방법의 개발은 지속되어야 한다.

• 대한영양사협회학술지
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• 제11권3호
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• pp.309-319
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• 2005

The purpose of this study was to compare the nutritional menu value of self-selected diet in Korean adults residing in different regions. Subjects were recruited and divided into three groups according to the districts where they lived, which included rural district(n=137), coastal district(n=100), and urban district(n=117). Subjects were interviewed using a general questionnaire and 24-hour recall method for dietary intake. The average age of the subjects were 57.5 years for rural district, 57.0 years for coastal district, and 47.9 years for urban district. The contents of energy, calcium, zinc, vitamin $B_2$, and vitamin E in self-selected diet were 76.1%, 60.1%, 73.1%, 68.6%, and 80.4% of RDAs, respectively. Index of nutritional quality(INQ) for calcium and vitamin $B_2$ was below in 1 in region groups. The contents of calcium and vitamin E in self-selected diet of rural and coastal districts were significantly lower than those of urban district. The numbers of food items in diet of rural, coastal, and urban district were 14.6, 15.3, 15.1 for breakfast, 16.5, 11.8, 17.0 for lunch, 14.9, 12.1, 15.1 for dinner, respectively. However, there was no significance in total food intake by regions. The food intakes from cereals, mushrooms, vegetables of rural district, that from fishes of coastal district, and those from sugars, milks, oils of urban district were the highest among three districts. The numbers of dish items in diet of rural, coastal, and urban district were 4.1, 4.4, 4.1 for breakfast, 4.5, 3.7, 4.4 for lunch, 4.0, 3.8, 4.2 for dinner, respectively. Especially, the number of food and dish items in lunch menu of coastal district was the lowest among region groups. According to these results, it could be suggested to add milk and its products in lunch menu of rural and coastal districts and to supplement fish and shellfish like anchovy in diet of rural and urban districts. And it is recommended to increase food and dish items in diets of three region groups.

• 동아시아식생활학회지
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• 제26권1호
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• pp.25-33
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• 2016

The study was performed to investigate the effects of whey protein-rich meal substitute added with vitamins, minerals, and lactobacillus powder probiotics on weight loss, body fat, and body composition in 24 female volunteers for 4 weeks. Whey protein-rich meal substitute was consumed with low-fat, high calcium milk (1% fat, 260 mg/200 mL) twice a day. Subjects submitted 3-day diet records and a life-style questionnaire before the study. During the study, subjects were required to turn in a diet record every day and consume the meal substitute formula in the metabolic ward at C university for 4 weeks. Anthropometric measurements were carried out weekly by Inbody 7.0. The dietary intake and anthropometric data were analyzed to compare changes before and after the study by paired t-test with SPSS version 23.0. The subjects were mostly early 20's and either overweight or obese and highly motivated to lose weight. Most of the subjects consumed three meals per day regularly and spent mostly 10~15 minutes for a meal. Their caloric intake was relatively low and decreased from 1,360 kcal at week 0 to 1,100 kcal after 4 weeks. However, total protein intake increased while carbohydrate and fat intakes decreased (p<0.05) after the trial. Nine vitamin intakes after the study improved compared to those before the study (p<0.05). After the study, subjects showed lower body weight (-1.8 kg), body fat (-0.94 kg), percent body fat (-0.86%), as well as waist circumference (-4.52 cm), hip circumference (-0.44 cm), waist hip ratio (-0.05), and triceps skinfold thickness (-2.39 mm) compared to those at week 0 (p<0.05). Muscle mass tended to be less compared to week 0, although there was no significant differences between weeks 0 and 4. In conclusion, diet trial with whey protein-rich meal substitute induced weight loss and positively changed body fat parameters and body composition.

• 한국식생활문화학회지
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• 제11권3호
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• pp.305-316
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• 1996

The purpose of this study was to find out how much they have several aspects of food related knowledge and eating behaviors of high school students in Sungnam area. The self-administrated questionnaire was used. The result were as follows: 1. The average height of boys and girls was $172.6{\pm}0.7$ cm and $156.3{\pm}1.5$ cm respectively. The average weights of them were $62.4{\pm}0.5$ kg (male) and $47.2{\pm}0.8$ kg (female). BMI (Body Mass Index) of them were $20.74{\pm}0.14$ (male), $18.82{\pm}0.28$ (female). The average age is 16.7 years old. 2. The 66.5% of the subjects were spent more than one-third of their pocket money in buying on light meals during three times a week. There were significant differences between income level of family. Boys spent more money on each meal than girls. And significant differences were also obserbed by their residence area and Bundang residences spent more in buying snacks. 3. The rate of skipping meals was 51.2% in boys compared with 68.0% in girls. The frequencies of buying snacks instead of main meal were high in girls. Time limits in eating may possibly be the main reason for skipping meals (59.8%), especially in the morning. Skipping a breakfast becomes general eating habits in high school students, because of pressure for time to go to school. 4. It is required that parents should be taught to prepare balanced lunch box for their children because the rate of students who prepared two lunch boxes are 49.4%. 5. The students took snacks once or twice a day. They usually bought snacks in school concessions (51.8%) and they selected items of snack instinctivly. The girls ate snacks during lunch break time (31.7%) and after dinner (23.6%). Boys ate snacks after dinner (29.1%). Preference of foods were different by sex. Boys preferred bread (31.7%), milk and otherdairy products (80.8%), cola and soda (42.0%) as their snacks between meals. Girls selected biscuit, chip, beverage, coffee as their snacks, frequently. 6. BMI value of the group who ate between meals more than three times a day was lower $(18.78{\pm}0.65)$ than that of the group who ate nothing between meals $(20.71{\pm}3.79)$. 7. As for the nutritional knowledge, the students generally had higher correct rate of answer about which nutritive components of food has (76.6%). But they had lower knowledge on questions of nutritive values in food (10.6%). There was a meaningful relation between favorite food and nutritional knowledge. In conclusion, there were some problems on nutritional knowledge and eating habits among the high school students. Therefore, it was required that girls should be learned to recognize the importance of breakfast and needed to select balanced meals and snacks. And it was required that the nutrition education should be complemented to motivate and improve practical eating behaviors.

• 대한임상검사과학회지
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• 제54권3호
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• pp.217-223
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• 2022

다양한 건강기능식품 중 프로바이오틱스는 전 세계적으로 가장 방대한 시장을 이루고 있으며, 장 건강이 우리 몸의 전반적인 면역작용을 조절하며 질병을 예방하고, 나아가 치료에 도움을 줄 수 있다는 연구결과에 따라 지속적으로 프로바이오틱스에 대한 관심이 증가하고 있다. 본 연구에서는 무화과식초가 가진 항균능을 이용하여 유제품과 드레싱 소스를 개발하여 그 선호도를 조사하고 대장세포에 미치는 영향을 분석하고자 하였다. 일반우유에 5% 무화과식초를 첨가하였을 때 마시는 요거트와 리코타치즈에 대한 가장 만족도가 높았다. 제조된 유제품에서 분리한 균은 Leuconostoc lactis이며 1.0×10⁷~1.0×10⁸ CFU/mL 이상의 세균수가 측정되어 식품의 기준 및 규격의 기준치를 만족하였다. 인체의 소장 상피세포와 흡사하게 증식되는 Caco-2 세포주에서 세포생존율을 관찰한 결과, 유청을 10% 농도로 처리하였을 때 세포생존율이 19% 가량 유의적으로 증가하였다. 농도별로 유청을 처리하였을 때 최대 58%의 항산화능을 보였다. 또한 유청에서 분리된 Leuconostoc lactis는 우수한 부착능이 관찰됨에 따라 장건강에 도움을 주는 유제품임을 확인하였다.