• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.114.1-114
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• 2003

A new isolate of rod-shaped virus was identified from grafted cactus, Gymnocalycium mihanovichii grafted onto Hylocereus trigonus, in Korea. The virus proved to be a new Tobamovirus and called previously as Tobamovirus-Ca for which we suggest the name Cactus mild mottle virus(CMMoV), because it produced systemic mild mosaic symptoms on its original host. CMMoV is distantly related to known species of the genus Tobamovirus on the basis of host range, serological and sequence analyses. Western blot analysis showed that CMMoV is serologically unrelated to Summons' Opuntia virus which is the only known species of the genus found in cactus plants. The 3'-terminal 2,910 nucleotides have been sequenced for the virus. The coat protein (CP) and movement protein (MP) genes encode 161 and 306 amino acids residues, respectively. The nucleotide and amino acid sequences of the CP were 39.6 ％ to 49.2 ％ and 26.4 ％ to 40.3 ％ identical to other tobamoviruses, respectively. The MP and 3' noncoding region shared 16.3 ％ to 23.3 ％ and 44.6 ％ to 63.4 ％ identities, respectively, with the members of the genus. Phylogenetic tree analysis of the CP gene revealed that CMMoV clusters with members of subgroup I of Tobamovirus. CMMoV particles contained genomic RNA along with two subgenomic RNAs, and this characteristics is common in the members of the subgroup II. This is the first information of sequence and comparative analysis of a Tobamovirus that infects cactus.

• Journal of the Society of Naval Architects of Korea
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• v.43 no.4 s.148
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• pp.467-475
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• 2006

Fatigue strength of hull structural steel plate, extracted from longitudinal bulkhead of a 17-year-old ore/oil carrier for renewal, was investigated in air and in artificial seawater condition. The surface of the plate was covered with corrosion pits and they proved to be crack initiation sites by fractography using SEM. From this research, it was found that the evaluation method for fatigue strength of virgin mild steel plates in air and in artificial seawater can also be applied to the evaluation of the fatigue strength of mild steel plates those were long-term exposed to a corrosive environment and their surfaces had been rusted intensively.

• Journal of Ocean Engineering and Technology
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• v.22 no.4
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• pp.27-33
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• 2008

One of the central problems in astudy of the coastal surface wave environment is predicting the transformation of waves as they propagate toward the shore. The transformation is mainly due to the existence of obstacles, such as breakwaters and vertical cylinders. In general, the types of wave transformation can be classified as follows: wave diffraction, reflection, transmission, scattering, radiation, et al. This research dealtwith wave transmission and dissipation problems for two dimensional irregular waves and vertical circular cylinders. Using the unsteady mild slope equation, a numerical model was developed to calculate the reflection and transmission of regular waves from a multiple-row circular breakwater and vertical cylinders. In addition, hydraulic model experiments were conducted with different values for the properties between tire piles and the opening ratio (distances) between the rows of the breakwater. It was found that the transmission coefficients decreased with a decrease in the opening ratio and an increase in the rows of vertical cylinders. A comparison between the results of hydraulic and numerical experiments showed reasonable agreement.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.24 no.2
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• pp.84-88
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• 2012

A numerical skill for effective treatment of inclined boundaries in a finite element method is introduced. A finite element method has been frequently used to simulate hydraulic phenomena in a coastal zone since it can be applied to irregular and complex geometry. In case elliptic partial equations are governing equations for a finite element model, however, there is a difficulty in treating boundary conditions properly for cases in which boundaries are vertically inclined. In this study, a method to treat such inclined boundaries using Bessel functions for a finite element method is introduced and compared with analytical solutions.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.411-412
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• 2022

In recent years, the number of foot disease patients is increasing due to bad walking habits and wearing shoes. With the development of shoes and living environment, no one can be relieved from foot disease. A study on the effect of shoe design characteristics on satisfaction and purchase intention when a patient with foot disease purchases orthopedic shoes. The shoe design characteristics consisted of functionality, convenience, and aesthetics. This study is conducted from various perspectives, such as frequency of participation in social activities, purpose of wearing, and status and characteristics of each foot disease. It is expected that the research results will lead to the development of orthopedic shoe designs that can be usefully worn by patients with orthopedic mild foot disease in their daily life, and to lead to practical use and satisfaction.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.143.3-144
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• 2003

A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased pepper crop in Chunchon, Korea. The isolate was biologically purified on Nicoticaa tabacum cv. Xanthi-nc by successive single local transfer steps, and propagated on N. tabacum cv. Samsun. PMMoV-Kr could systemically infect on N. glauca, N. benthmiana, N. occidentalis and Lycopersicon esculentum, which is typical of known isolates of PMMoV. PMMoV-Kr belongs to the pathotype P1,2 based on pepper-tobamoviral indicator experiments; Capsicn chinone harboring L3 gene revealed resistant (necrotic local lesion on inoculated leaf, HR) whereas L+, L1 and L2 pepper plants expressed susceptible reactions of mosaic systemic symptoms for the isolate. To confirm the pathology and delineate symptom determinant of the isolate, full-length cDNAs of PMMoV-Kr were amplified by RT-PCR with a primer set corresponding to the 5'- and 3'-ends of PMMoV. The RT-PCR molecules amplified from genome RNA of the isolate was cloned into the pUC18 vector. Full-length cDNA clones constructed under the control of the T7 RNA promoter could be successfully transcribed to produce in vitro transcript RNA. Infectivity of the capped transcripts and its progeny virus was verified by Western blot and RT-PCR analyses.

• Research in Plant Disease
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• v.25 no.4
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• pp.226-232
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• 2019

Among more than 30 viruses infecting strawberry, aphid-transmitted viruses including Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV), and Strawberry vein banding virus (SVBV) have been considered as the most economically important viruses of strawberry in the world. To determine the incidence of these four viruses in major Korean strawberry cultivars, field surveys were conducted in major production areas during 2018-2019. In our surveys, SMYEV and SMoV were detected with low infection rates of 0.7% and 1.3%, respectively, whereas SCV and SVBV were not detected. No obvious symptoms were observed in the strawberry plants infected by SMYEV or SMoV. Since no sequences of SMoV Korean isolates have been reported, we initially determined nucleotide sequence of the 3' untranslated region (UTR) of seven SMoV isolates obtained during the surveys. The 3' UTR sequences (782 nt) of seven Korean isolates were phylogenetically compared with those of the previously reported SMoV isolates. Phylogenetic analysis revealed that most Korean isolates are closely related to Canadian isolates and only little evolutionary differentiation occurred among the Koreans isolates. This might be due to the low incidence of SMoV in strawberry in Korea.

• Journal of Animal Environmental Science
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• v.21 no.2
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• pp.55-64
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• 2015

This survey was conducted to give the basic information and temperature and humidity variation by cooling systems in dairy farms in Korea. A total of 36 farms were surveyed in Gyeonggi province with the general information of farms such as milk production, and milk quality, types of cooling systems, and its operation method of dairy farms. All of surveyed farms have cooling fan and some have 1 more cooling systems such as sprinkler, foggy systems, ventilation duct, and shower system. Although the indoor THI of cow house was decreased by either ventilation duct or foggy system during hot season, the range belongs to mild heat stress scale for dairy cows.

• Bulletin of the Korean Chemical Society
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• v.17 no.9
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• pp.854-857
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• 1996

Absorption and luminescence spectra of Eu3+ (aquo) and the two different 1: 3 Eu3+: ligand systems in aqueous solutions are measured under mild acidic pH condition. The oxydiacetate (ODA) and dipicolinate (DPA) ligands, forming the similar geometric complexes, are used in this work. The three intensity parameters, Ωλ (λ=2, 4, and 6) are empirically determined by applying the Judd-Ofelt theorem to the oscillator strengths of the six absorption bands arising from the ground 7F0 state. Among the three intensity parameters, the Ω2 is found to response markedly to a miner change in the ligand environment via the 7F0→5D0 transition. In addition, the relative oscillator strengths of the four luminescence bands in the visible region, assigned to the 5D0→7FJ (J=1, 2, 3 and 4) transitions are obtained to investigate their sensitivity to the ligand environment. Among the four bands, the 610 nm band, attributed to the 5D0→7F2 transition, shows hypersensitivity in the luminescence.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.1
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• pp.113-117
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• 2003

This study was performed to investigate the anti-depression effect of herbal extracts (Rehmanniae Radix Preparat, Corni Fructus, Lycii Fructus, Pinelliae Rhizoma, Nelumbinis Semen) in the chronic mild stressed rat. After adaptation period for 2 weeks, each herbal extract was administered to the rats divided 5 groups. For comparison, St. John's wart was administered to another group in the same environment. The chronic mild stress was induced to rats by overnight illumination for 2 days before the forced swimming test. Struggling time, first latency and rest duration were calculated during the forced swimming test to evaluate the anti-depressant effect. The results were as follows: 1. In the result of evaluation of the struggling time, there was no significance in Pinelliae Rhizoma (-1.1%), Corni Fructus (-3.9%), Rehmanniae Radix Preparat (15.2%), Lycii Fructus (4.9%), St. John's wart (25.2%). There was a significant increase only in Nelumbinis Semen (43.9%) (P=0.0037). 2. In the result of evaluation of the first latency time, there was no significance in Lycii Fructus (-21.37%), Rehmanniae Radix Preparat (38.4%), Pinelliae Rhizoma (65.5%), Corni Fructus (29.2%), St. John's wart (75.8%). There was a significant increase only in Nelumbinis Semen (90.2%) (P=0.0116). 3. In the result of evaluation of the first rest duration, there was no change in St. John's wart, and Decrease was shown in Pinelliae Rhizoma (62.4%), Corni Fructus (31.6%) Rehmanniae Radix Preparat (63.1 %), Lycii Fructus (12.4%), Nelumbinis Semen (59.0%), but there was no significance in all. 4. Nelumbinis Semen is considered to have a superior anti-depression effect than the other herbal extracts, and to have a superior and-depression effect than St. John's wart being used commonly.