• 식물병연구
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• 제10권4호
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• pp.241-247
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• 2004

Cucumber mosaic virus(CMV)-Paf 계통에 포함된 satellite RNA(Paf-satRNA)는 CMV의 병징을 완화시키는 약독병징 관련 유전자로 작용하였다(Choi 등, 2001). 이 연구는 Paf-satRNA의 약독병징 관련 유전자의 도메인을 확인하기 위하여, 고추에서 chlorosis 병징을 발현하는 PepY-satRNA와 키메라 satRNA를 구축하여 분석하였다. 두 종의 satRNA의 염기서열을 비교한 결과, 분자크기가 큰 PepY-satRNA에서 10염기의 삽입이 발견되기는 하였지만, 양 말단영역의 염기는 비교적 안정된 conserved sequence를 보였다. 그러나 이들 satRNA의 중간영역에 존재하는 염기서열, 즉 5' 말단의 81번째 염기로부터 113번째, 그리고 183번째 염기부터 265번째 염기까지의 영역에서는 많은 변화를 나타냈다. 약독병징과 관련된 도메인을 확인하기 위하여 구축한 각 satRNA 및 키메라 satRNA의 cDNA로부터 transcript RNA를 전사시키고, 전사된 각 satRNA transcript를 CMV-Fny의 게놈RNA1, RNA2 및 RNA3의 transcript와 혼합한 후 N. benthamiana에 접종하였다. 그 결과 RT-PCR에 의해서 모든 satRNA-cDNA로부터 전사된 transcript의 감염성이 확인되었으며, Paf-satRNA 및 키메라 Paf(H/N)-satRNA와 PepY(N/A)-satRNA를 접종한 N. benthamiana에서는 모두 약한 모자이크 또는 무병징 감염의 특성을 보였다. 이와는 대조적으로 PepY-satRNA 및 키메라 PepY(H/N)-satRNA와 Paf(N/A)-satRNA를 접종한 식물에서는 전형적인 모자이크 증상과 식물체의 위축을 동반하였다. 이들 각 키메라 satRNA에 감염된 N. benthamiana를 접종원으로 고추에 접종한 결과, Paf-satRNA와 혼합한 CMV-Fny를 접종한 고추에서는 무병징에 가까운 약한 모자이크 증상이 발현되었고, PepY-satRNA를 접종한 고추는 뚜렷한 chlorosis의 모자이크 증상이 발현되었다. 한편 이들 두 종 satRNA의 키메라, Paf(H/N)-satRNA와 PepY(N/A)-satRNA를 접종한 고추에서는 모두 약한 모자이크 또는 무병징 감염의 특성을 보였고, PepY(H/N)-satRNA와 Paf(N/A)-satRNA를 접종한 식물에서는 전형적인 chlorosis의 모자이크 증상과 식물체의 위축을 동반하였다. 이와 같은 결과를 종합해 보았을 때, N. benthamiana에서와 마찬가지로 Paf-satRNA의 약독병징과 관련된 유전자의 도메인은 HpaI-NarI 영역에 존재한다는 것을 나타냈다.

• Corrosion Science and Technology
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• 제17권5호
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• pp.249-256
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• 2018

Metallic structures in the oil and gas production undergo severe degradation due to sweet and sour corrosion caused by the presence of $CO_2$ and $H_2S$ in the fluid environment. The corrosion behavior of 304 austenitic stainless was investigated in the presence of varying concentrations of $CO_2$ or $H_2S$ and $CO_2+H_2S$ to understand the effect of the parameters either individually or in combination. Potentiodynamic polarization study revealed that a small amount of $CO_2$ aided in the formation of calcareous deposit of protective layer on passive film of 304 steel, while increase in $CO_2$ concentration ruptured the layer resulting in sweet corrosion. The presence of $S^{2-}$ damaged the passive and protective layer of the steel and higher levels increased the degradation rate. Electrochemical impedance studies revealed lower polarization resistance and impedance at higher concentration of $CO_2$ or $H_2S$, supporting the outcomes of polarization study. XRD analysis revealed different types of iron carbides and iron sulphides corresponding to sweet and sour corrosion as the corrosion products, respectively. SEM analysis revealed the presence of uniform, localized and sulphide cracking in sour corrosion and general corrosion with protective carbide layer amid for sweet corrosion.

• 한국해양환경ㆍ에너지학회지
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• 제8권1호
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• pp.23-30
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• 2005

특정 해역에 부방파제를 설치하는 경우 예상되는 정온도의 평가를 위한 수치해법을 제안하고, 이를 바탕으로 마산 부근의 원전항에 설치예정인 부방파제의 효과를 검토하였다. 수치모형은 항 내 정온도를 평가하는 데 널리 사용되는 완경사 방정식을 이용하였고 산란 항을 도입하여 부방파제와 같은 폭이 얇은 구조물에 의한 산란 파를 반영하도록 하였다. 부방파제의 도입이 결정된 동방파제의 효과를 수치모의 하였고, 착저식으로 하는 경우와 비교하였다. 부방파제의 부분투과 등 구조물이 파랑에 미치는 영향을 적극적으로 고려하는 본 수치해법은 부방파제 효과의 정량적 평가에 효율적으로 활용될 수 있다.

• Smart Structures and Systems
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• 제19권3호
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• pp.299-307
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• 2017

The magneto-electro-elastic (MEE) material under thermal environment exhibits pyroelectric and pyromagnetic coefficients resulting in pyroelectric and pyromagnetic effects. The pyroelectric and pyromagnetic effects on the behavior of multiphase MEE sensors bonded on top surface of a mild steel beam under thermal environment is presented in this paper. The aim of the study is to find out how samples having different volume fractions of the multiphase MEE composite behave in sensor applications. This is studied at optimal location on the beam, where the maximum electric and magnetic potentials are induced due to pyroelectric and pyromagnetic effects under clamped-free and clamped-clamped boundary conditions. The sensor which is bonded on the top surface of the beam is modeled using 8-node brick element. The MEE sensor bonded on mild steel beam is subjected to uniform temperature rise of 50K. It is assumed that beam and sensor is perfectly bonded to each other. The maximum pyroelectric and pyromagnetic effects on electric and magnetic potentials are observed when volume fraction is ${\nu}_f=0.2$. The boundary conditions significantly influence the pyroelectric and pyromagnetic effects on electric and magnetic potentials.

• 인간식물환경학회지
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• 제21권6호
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• pp.515-521
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• 2018

The purpose of this study was to investigate the effects of horticultural activities with reminiscence materials and singing songs on the ability to recall words and depression in the elderly with mild dementia. A total of 20 sessions of a horticultural therapy program was held once or twice a week from July 14, 2015 to November 3, 2015 for the elderly with mind dementia at the social welfare center in D city. The subjects of this study were 13 women and the mean age was 81.2 years. The ability to recall words significantly improved from $8.6{\pm}1.8$ before the program to $9.4{\pm}0.6$ after the program (p=.012). However, there was no significant difference in the score of depression after the program. Synergistic effects were generated by applying horticultural activities, singing and reminiscence therapy activities simultaneously, and it was effective in recalling memories. As emotional functions were associated with hormones in vivo, there were emotional ups and downs at each session and short-term alternative therapies were not sufficient to completely eliminate neurotoxic substances caused by dementia.

• 한국정보통신학회논문지
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• 제26권7호
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• pp.1019-1025
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• 2022

최근 들어 잘못된 보행 습관과 생활환경으로 인한 족부 질환자들이 늘어나고 있다. 신발디자인 특성에 따른 경증 족부질환 환자가 정형 신발 구매시 만족도와 구매 의도에 어떠한 영향을 미치는지 살펴본다. 신발 디자인 특성은 기능성, 편의성, 심미성으로 구성하였다. 심미성이 정형 신발 구매 시 영향을 미치는 것으로 나타났다. 기능성과 편의성의 요소가 영향이 적더라도 심미성이 높다면 구매의도와 만족도에 영향을 준다. 경증 족부질환별 사회활동 참여 빈도와 착용 목적에 따라 디자인 또한 다양한 요소들이 요구된다. 연구 결과를 통해 알아본 정형 신발디자인 특성 요인을 더욱 보완하여 경증 족부질환 환자들이 일상생활에서 착용할 수 있는 유용한 정형 신발 디자인 개발과 현실적인 활용과 만족으로 이어지길 기대한다.

• The Plant Pathology Journal
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• 제37권6호
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• pp.632-640
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• 2021

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material - chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2001년도 추계학술대회 논문집
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• pp.117-121
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• 2001

For the effective development or preservation of Tokdo, the natural environments in the ambient sea area should be well investigated. The wave deformations and wave breaking in the vicinity have much affected the bottom morphology of Tokdo as well as its ecological environment. The present study investigates the wave deformations and wave breaking through a numerical model. The final goal is to provide the fundamental wave data for the effective development or preservation of Tokdo in future. The extended mild slope equation was applied to Tokdo sea area for three different deep water wave conditions (S, SSE, NNE directions). The results showed that for the S and SSE directions the wave heights in the area between the east island and the west island were very low with the level of 1~2m, but for the NNE direction they appeared pretty high with 3~4m, In the sea area near the northwest of west island, the wave heights were low to be 1~3m for all three directions of deep water wave.

• Tribology and Lubricants
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• 제20권2호
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• pp.91-96
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• 2004

Wear behavior of self-mated stainless steels in NaCl solution has been investigated. The experiment was done in the corrosive liquid of which NaCl concentration of $0\~3\%$ and temperature of $15\~90^{\circ}C$. Two kinds of wear type were observed: one is 'severe wear' type which shows gradually increasing wear volume with increasing sliding distances, the other is 'mild wear' type which shows change of wear rate from high value to low at transition distance. The specific wear rate in severe wear type was not sensitive to the liquid temperature and concentration of NaCl but stable at value of $1\times10^{-3}\;mm^3$ approximately.

• Bulletin of the Korean Chemical Society
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• 제35권3호
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• pp.715-720
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• 2014

A Cu(II) complex with an three nitrogens and one sulfur coordination environment was synthesized and characterized. Its redox potential was observed at 0.483 V vs. NHE, very similar to that of a Cu-containing fungal enzyme, galactose oxidase, which catalyzes the oxidation of alcohols to corresponding aldehydes with the concomitant reduction of molecular oxygen to water. The Cu(II) complex selectively oxidizes the benzylic alcohols using TEMPO/$O_2$ under mild reaction conditions to corresponding aldehydes without forming any over-oxidation product. Moreover, the catalyst can be recovered and reused multiple times for further oxidation reactions, thus minimizing the waste generation.