• Nutrition Research and Practice
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• v.8 no.5
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• pp.521-525
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• 2014

BACKGROUND/OBJECTIVES: Artemisinin (AT), an active compound in Arternisia annua, is well known as an anti-malaria drug. It is also known to have several effects including anti-oxidant, anti-inflammation, and anti-cancer activities. To date, the effect of AT on vascular disorders has not been studied. In this study, we investigated the effects of AT on the migration and proliferation of vascular smooth muscle cells (VSMC) stimulated by platelet-derived growth factor BB (PDGF-BB). MATERIALS/METHODS: Aortic smooth muscle cells were isolated from Sprague-Dawley rats. PDGF-BB stimulated VSMC migration was measured by the scratch wound healing assay and the Boyden chamber assay. Cell viability was determined by using an EZ-Cytox Cell Viability Assay Kit. The production of reactive oxygen species (ROS) in PDGF-BB stimulated VSMC was measured through $H_2DCF$-DA staining. We also determined the expression levels of signal proteins relevant to ROS, including measures of extracellular signal-regulated kinase (ERK) 1/2 measured by western blot analysis and matrix metalloproteinase (MMP) 9 measured by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: AT ($10{\mu}M$ and $30{\mu}M$) significantly reduced the proliferation and migration of PDGF-BB stimulated VSMC in a dose-dependent manner. The production of ROS, normally induced by PDGF-BB, is reduced by treatment with AT at both concentrations. PDGF-BB stimulated VSMC treated with AT ($10{\mu}M$ and $30{\mu}M$) have reduced phosphorylation of ERK1/2 and inhibited MMP9 expression compared to untreated PDGF-BB stimulated VSMC. CONCLUSIONS: We suggest, based on these results, that AT may exert an anti-atherosclerotic effect on PDGF-BB stimulated VSMCs by inhibiting their proliferation and migration through down-regulation of ERK1/2 and MMP9 phosphorylation.

• Molecules and Cells
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• v.20 no.2
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• pp.263-270
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• 2005

Transactivation of EGF-receptor (EGFR) by G-protein coupled receptors (GPCRs) is emerging as an important pathway in cell proliferation, which plays a crucial role in the development of atherosclerotic lesion. Angiotensin II (Ang II) has been identified to have a major role in the formation of atherosclerotic lesions, although the underlying mechanisms remain largely unclear. We hypothesize that Ang II promotes the proliferation and migration of smooth muscle cells through the release of heparin-binding epidermal growth factor like growth factor (HB-EGF), transactivation of EGFR and activation of Akt and Erk 1/2, with matrix metalloproteases (MMPs) playing a dispensable role. Primary rat aortic smooth muscle cells were used in this study. Smooth muscle cells rendered quiescent by serum deprivation for 12 h were treated with Ang II (100 nM) in the presence of either GM6001 ($20{\mu}M$), a specific inhibitor of MMPs or AG1478 ($10{\mu}M$), an inhibitor of EGFR. The levels of phosphorylation of EGFR, Akt and Erk 1/2 were assessed in the cell lysates. Inhibition of MMPs by GM6001 significantly attenuated Ang II-stimulated phosphorylation of EGFR, suggesting that MMPs may be involved in the transactivation of EGFR by Ang II receptor. Furthermore Ang II-stimulated proliferation and migration of smooth muscle cells were significantly blunted by inhibiting MMPs and EGFR and applying HB-EGF neutralization antibody, indicating that MMPs, HB-EGF and EGFR activation is necessary for Ang-II stimulated migration and proliferation of smooth muscle cells. Our results suggest that inhibition of MMPs may represent one of the strategies to counter the mitogenic and motogenic effects of Ang II on smooth muscle cells and thereby prevent the formation and development of atherosclerotic lesions.

• Journal of Korean Society of Rural Planning
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• v.23 no.2
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• pp.43-54
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• 2017

When an age of low growth and population decline, population migration plays an important role in spatial structure of region. There have been many researches on migration and regional spatial structure. The purpose of this study is to examine the changes of Gwangju and Jeonnam region's spatial structure and central area using social network analysis methods. For analysis it was used that population and migration data and passenger OD(Origin and Destination) travel data released by Statistics Korea and Korea Transport Database(KTDB). Using Gephi 0.8.2, migration and passenger OD networks were visualized, and this describe network flow and density. The results of the network centrality analysis show that the most populated village is not always network center though population mass is an important factor of central places. The average eigenvector centrality of 2010 migration is the lowest during 2005-2015, and it means few regions have high centralities. When comparing migration and travel networks, travel data is more effective than migration data in determining the central location considering spatial functions.

• Journal of Agricultural Extension & Community Development
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• v.18 no.1
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• pp.1-33
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• 2011

In-migration has long been recognized as on important factor in rural development. Its impact can be expected to increase even further as the elderly population continues to grow. Recently with the retirement of baby boomers, postretirement moves have become increasingly important issue in rural Korea. Because the consequences of rural in-migration are often most pronounced at the local or regional level, rural planners are among the many scholars and practitioners seeking to understand the patterns and consequences of in-migration. At the same time, however, planners have sometimes overlooked basic research on the causes and determinants of mobility, and on the relationships between retirement migration and other socioeconomic processes. The papers aims to explore the reviews of rural in-migration studies and its practical implications, providing an interdisciplinary review of the most important studies published between 1990-2009. Through the literature review of in-migration, this study suggests that main arguments for rural in-imgration studies such as rural populations, counterurbanization, rural businesses incubator and rural amenity be emphasized to vitalize and diversify rural economies.

• BMB Reports
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• v.57 no.5
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• pp.250-255
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• 2024

Due to their stem-like characteristics and immunosuppressive properties, Mesenchymal stem cells (MSCs) offer remarkable potential in regenerative medicine. Much effort has been devoted to enhancing the efficacy of MSC therapy by enhancing MSC migration. In this study, we identified deubiquitinase BRCA1-associated protein 1 (BAP1) as an inhibitor of MSC migration. Using deubiquitinase siRNA library screening based on an in vitro wound healing assay, we found that silencing BAP1 significantly augmented MSC migration. Conversely, BAP1 overexpression reduced the migration and invasion capabilities of MSCs. BAP1 depletion in MSCs upregulates ERK phosphorylation, thereby increasing the expression of the migration factor, osteopontin. Further examination revealed that BAP1 interacts with phosphorylated ERK1/2, deubiquitinating their ubiquitins, and thus attenuating the ERK signaling pathway. Overall, our study highlights the critical role of BAP1 in regulating MSC migration through its deubiquitinase activity, and suggests a novel approach to improve the therapeutic potential of MSCs in regenerative medicine.

• Journal of Life Science
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• v.24 no.4
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• pp.337-342
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• 2014

Resveratrol (RSV), a natural polyphenolic compound, is a modulator for cell division and cell migration, and has diverse beneficial properties. Angiogenin (ANG) and vascular endothelial growth factor (VEGF) are considered to be important mechanisms for cell proliferation, angiogenesis, the formation of tubular structures, and migration. In this study, we investigated whether RSV has a migratory effect in HeLa cells. When cells were treated with $0{\sim}50{\mu}M$ of RSV for 24 hr, the expression of ANG and VEGF was significantly increased in a dose dependent manner measured by real-time PCR. Similarly, we performed time dependent experiments for $50{\mu}M$ RSV treated cells and identified the optimized time at 24 hr. The increased expression in RSV treated cells was confirmed by Western blot analysis. To examine the toxic effects of RSV at the determined conditions, MTT assays were performed. The viabilities were unchanged for $0{\sim}50{\mu}M$ RSV treated cells, while they decreased at $100{\mu}M$ RSV. To examine the effect of migration in RSV treated cells, we performed a wound-healing assay. The migratory rates were significantly enhanced in the RSV treated group. In this study, we found that RSV induces an increase in the expression of migration factors ANG, VEGF, and enhances cell migration for the determined conditions.

• Journal of Information Technology Applications and Management
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• v.15 no.3
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• pp.1-25
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• 2008

IT platform migration to open systems (IPMO) bears a great deal of risk over all the associated processes, in terms of a major IT investment. Hence it requires empirical data and references for decision making. Although there have been a number of published papers encouraging or discouraging IPMO, the studies that deliver useful empirical evidence for IPMO decisions are rare. The obvious first step to resolve this problem would be to gain lessons from the organizations who experienced IPMO. Based on the Delphi study, we examine both the pre and post evaluations on IPMO benefits and risks and analyze the underlying reasons of different evaluations from different stages. Our results identify the most important factor the organizations should seriously consider, and which factor is easy to neglect at the ex-ante appraisal stage.

• International Journal of Concrete Structures and Materials
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• v.8 no.4
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• pp.269-278
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• 2014

When concrete is being transported through a pipe, the lubrication layer is formed at the interface between concrete and the pipe wall and is the major factor facilitating concrete pumping. A possible mechanism that illustrates to the formation of the layer is the shear-induced particle migration and determining the rheological parameters is a paramount factor to simulate the concrete flow in pipe. In this study, numerical simulations considering various rheological models in the shear-induced particle migration were conducted and compared with 170 m full-scale pumping tests. It was found that the multimodal viscosity model representing concrete as a three-phase suspension consisting of cement paste, sand and gravel can accurately simulate the lubrication layer. Moreover, considering the particle shape effects of concrete constituents with increased intrinsic viscosity can more exactly predict the pipe flow of pumped concrete.

• Water for future
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• v.25 no.2
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• pp.79-87
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• 1992

This study is a model on the Migration Characteristics which developed by using the equations for conservation of mass, momentum and for lateral stability of the streambed, an the model can be examined for magnitude and location of near-bank bed scour as well as rates and direction of meander migration in which sine-Wave type rivers(SWR) of the small sinuosity. It is evident from this study that the transverse bed slope factor B' and transverse mass flux factor $ play significient roles, and show reasonable that the values are B'=4.0 and $=0.4 respectively . It will be a useful guide in planning, design, construction, and development of SWR river-basin projects.

• Development and Reproduction
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• v.27 no.4
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• pp.221-226
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• 2023

A stem cell niche provides an environment that governs stem cell maintenance and division. Thus, the development of a proper niche is of prime importance to stem cell behaviors. Mechanisms of niche development are beginning to be revealed in the Drosophila male gonad. Niche cells are initially dispersed throughout the gonad, then assemble at its apical tip through the anterior migration of posteriorly located niche cells. The molecular mechanisms of this migration and assembly are still poorly understood. Here we show evidence suggesting that Lin28, an RNA-binding protein and regulator of let7 genesis, might be an intrinsic factor for the anterior migration of niche cells. We found that a dispersed, ectopic niche, a phenotype observed with anterior migration defects, occurs in lin28 mutant gonads. This phenotype is rescued by expression of lin28 in the niche cells. These findings suggest that Lin28 might be required for the anterior migration of niche cells.