• International Journal of Oral Biology
• /
• 제43권2호
• /
• pp.69-76
• /
• 2018

Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelialmesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.

• Journal of Acupuncture Research
• /
• 제22권2호
• /
• pp.171-180
• /
• 2005

Background & Objective : Angiogenesis consists of the proliferation, migration, and differentiation of endothelial cells, and angiogenic factors and matrix protein interactions modulate this process. The aim of this study was to determine whether Puerariae radix could induce angiogenic activity in human umbilical vein endothelial cells (HUVECs). Methods: The angiogenic activity of Puerariae radix were evaluated by using BrdU assay, chemotactic migration assay, tube formation assay, measurement of bFGF in HUVECs, and Matrigel plug assay in mice. Results : Puerariae radix significantly increased HUVECs proliferation in a dose-dependent manner. In addition, Puerariae radix increased migration and tube-like formation in HUVECs. Interestingly,the expression of basic fibroblast growth factor (bFGF), an angiogenesis-stimulating growth factor, was dose-dependently increased by Puerariae radix. The angiogenic activity of Puerariae radix was confirmed using an in vivo Matrigel angiogenesis model, showing promotion of blood vessel formation. Conclusion : Puerariae radix significantly induces angiogenesis in vitro and in vivo. These results suggest that Puerariae radix is a potent angiogenic agent, and a promising drug, for the induction of neovascularization.

• Journal of Ginseng Research
• /
• 제43권3호
• /
• pp.488-495
• /
• 2019

Background: Timosaponin AIII (TA3) is a steroidal saponin extracted from Anemarrhena asphodeloides. Here, we investigated the anticancer effects of TA3 in MG63 human osteosarcoma cells. TA3 attenuates migration and invasion of MG63 cells via regulations of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, which are involved with cancer metastasis in various cancer cells. TA3 reduced enzymatic activities and transcriptional expressions of MMP-2 and MMP-9 in MG63 cells. TA3 also inhibited Src, focal adhesion kinase, extracellular signal-regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, ${\beta}-catenin$, and cAMP response element binding signaling, which regulate migration and invasion of cells. TA3 induced apoptosis of MG63 cells via regulations of caspase-3, caspase-7, and poly(ADP-ribose) polymerase (PARP). Then, we tested several ginsenosides to be used in combination with TA3 for the synergistic anticancer effects. We found that ginsenosides Rb1 and Rc have synergistic effects on TA3-induced apoptosis in MG63 cells. Methods: We investigated the anticancer effects of TA3 and synergistic effects of various ginseng saponins on TA3-induced apoptosis in MG63 cells. To test antimetastatic effects, we performed wound healing migration assay, Boyden chamber invasion assays, gelatin zymography assay, and Western blot analysis. Annexin V/PI staining apoptosis assay was performed to determine the apoptotic effect of TA3 and ginsenosides. Results: TA3 attenuated migration and invasion of MG63 cells and induced apoptosis of MG63 cells. Ginsenosides Rb1 and Rc showed the synergistic effects on TA3-induced apoptosis in MG63 cells. Conclusions: The results strongly suggest that the combination of TA3 and the two ginsenosides Rb1 and Rc may be a strong candidate for the effective antiosteosarcoma agent.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2003년도 춘계학술발표논문집 (중)
• /
• pp.907-910
• /
• 2003

본 논문에서는 에이전트의 효율적 이주를 제공하기 위한 전역 디렉토리 스킴을 제안한다. 제안된 스킴은 동적으로 삯은 변경을 갖는 분산 시스템에서 에이전트의 서비스 수행을 위한 에이전트 및 환경에 대한 트래킹을 통해 효율적인 이동 에이전트의 이주를 지원하는 것을 목적으로 한다. 본 연구에서는 이를 위해 대표적인 이동에이전트 시스템인 IBM's Aglets Software Development Kit을 확장하여 에이전트 수행 환경에 대한 모니터링 모듈을 구현하고 이를 기반으로 기존의 마스터-슬래이브 형태의 이동 에이전트 시스템을 확장하여 구현하였다.

• 한국멀티미디어학회:학술대회논문집
• /
• 한국멀티미디어학회 2004년도 춘계학술발표대회논문집
• /
• pp.851-854
• /
• 2004

분산 유전 알고리즘은 외판원 문제를 해결하는데 효율적이고 적용하기 쉬운 알고리즘이다. 그러나 다중 후보해를 가진 분산 유전 알고리즘을 수행할 때, 효율성과 정확성에 영향을 주는 많은 요소들이 고려되어야 한다. 후보해의 크기를 얼마로 할 것인지, 이주의 비율 및 횟수는 어떻게 할 것인지와 그리고 어떤 개체들을 선택해서, 어떤 후보해 개체와 교환할 것인가가 중요하게 고려되어야 한다. 따라서 본 논문에서는 이주해야 할 개체의 크기를 동적으로 변경하면서 교환하는 방법과 또한 개체들이 이주되어야 할 위치를 결정하는 이주 정책을 개발하고 실험하였다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XIII)
• /
• pp.644-647
• /
• 2003

Materials currently used as an injectable bulking agent in the dermatologic and urologic fields revealed several drawbacks such as particles migration, inflammatory reaction, allergic reaction, rapid volume shrinkage, and necessity of a donor site. In this study, we have developed injectable biomaterial comprising poly (DL- lactide-co-glycolide)(PLGA) and hyaluronic acid gel to overcome these problems. PLGA is a biocompatible synthetic material and hyaluronic acid is a common substance found in living organisms. We examined the feasibility of injection through needle and tested biocompatibility in animal model. After transplantation, injected sites and distant organs were examined histologically to verify a new tissue formation, inflammation, and particles migrations. Injected volume was maintained approximately 80 percent for 2 months. Results demonstrated that the developed material was injectable through various gauges of needles and induced a new bulking tissue formation without serious inflammatory reaction.

• International Journal of Control, Automation, and Systems
• /
• 제2권3호
• /
• pp.333-342
• /
• 2004

This paper presents a framework for the self-organization of swarm systems based on coupled nonlinear oscillators (CNOs). In this scheme, multiple agents in a swarm self-organize to flock and arrange themselves as a group using CNOs, which are able to keep a certain distance by the attractive and repulsive forces among different agents. A theoretical approach of flocking behavior by CNOs and a design guideline of CNO parameters are proposed. Finally, the formation scenario for cooperative multi-agent groups is investigated to demonstrate group behaviors such as aggregation, migration, homing and so on. The task for each group in this scenario is to perform a series of processes such as gathering into a whole group or splitting into two groups, and then to return to the base while avoiding collision with agents in different groups and maintaining the formation of each group.

• 한국통신학회논문지
• /
• 제29권3B호
• /
• pp.314-324
• /
• 2004

최근 초고속 인터넷 사용자는 꾸준하게 증가해서 천만을 넘어섰다. 그러나 사용자 액세스 네트워크(xDSL, Cable, B-WLL 등)가 다양해지고, 고부가가치의 유무선 컨텐츠 서비스가 발전함에 따라 기존의 단순 접속만을 하던 서비스는 네트워크 대역폭 보장 및 지연 방지 등의 한계에 부딪히게 되었다. 본 논문에서 제안한 에이전트 기반의 인터네트워킹 플랫폼(Agent based Inter Networking Platform)은 계층적 객체지향 시스템으로 사용자 요구(User on Demand)에 따른 다양한 서비스(Access QoS, 맞춤 응용 서비스, 보안 서비스)를 제공할 수 있다. 이 시스템은 사용자 에이전트와 에이전트 지역관리 서버 및 중앙관리 서버로 나뉘어져 있으며, 중앙 관리 서버는 사용자 에이전트로부터 할당된 대역폭, 현재 전송 속도, 지연, 현재 서비스 등의 상태정보를 주기적으로 받아서 처리한 후, 정책을 세우고, 이를 토대로 작성된 사용자 개개인의 프로파일에 따라 서비스를 하는 것이다. 시스템의 이식성과 통합을 고려해 객체지향의 모델링 기법을 사용하교 계층화된 서버를 통하여 보안에 강한 시스템을 구현하는 방안을 제시하고자 한다.

• 정보처리학회논문지D
• /
• 제8D권4호
• /
• pp.439-450
• /
• 2001

Mobile agent is one of the promising ways of overcoming network load and latency. It is also a new software paradigm including those concepts of client/server, applet/servelet, and code-on-demand. In this paper, a new mobile agent concept with flexible replay scheme is proposed, which can deal with embarrassing situation when replying results should be accomplished in various distributed applications. For example, they are 1) a burden of bulky result, 2) a need of different replay scheme dependent on work type, 3) connection failure to the target node, and 4) a need of fast migration to next node to keep the itinerary. Regarding the source node, there may be another situation that it wants to be aware of whether its wi가 is completed or not. Three kinds of reply schemes are proposed for dealing flexibly with such situations. They are based on priority reply list where nodes to be replied are stored according to their priorities. An experimental supply chain management model using the proposed reply schemes is designed and implemented. It is one of good distributed applications appropriate for our reply schemes, because it requires different reply schemes according to work types and it is important to gather required information in time.

• Journal of Microbiology and Biotechnology
• /
• 제28권8호
• /
• pp.1332-1338
• /
• 2018

In the course of studies to discover natural products with anti-angiogenic properties, two cyclic octapeptides, octaminomycins A (1) and B (2), were isolated from the cultures of Streptomyces sp. RK85-270. Octaminomycins suppressed the vascular endothelial growth factor (VEGF)-induced proliferation, adhesion, tube formation, migration, and invasion of HUVECs. Anti-angiogenic activity was futher confirmed in vivo by the chicken chorioallantoic membrane assay. We also identified that 1 and 2 inhibited the phosphorylation of VEGF receptor 2, AKT, and ERK1/2 and the expression and activities of MMP-2 and MMP-9. These results suggest that 1 and 2 may serve as potential scaffolds for the development of therapeutic agents to angiogenesis-dependent diseases.