• Title/Summary/Keyword: Microscopic observation

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A study on SiC crystal growth by sublimation process using resistance heating method (저항가열 방식을 적용한 승화법에 의한 SiC 결정 성장에 대한 연구)

  • Kang, Seung Min
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.3
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    • pp.85-92
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    • 2015
  • SiC crystals are well known for their true potential as high power devices and their crystal growth activity is actively carried out in domestic as well as in abroad. Until now the process to grow this crystal has been done by sublimation technique using radio frequency induction heating method. However in order to get better quality of SiC crystals, the stability of temperature is needed because SiC crystal tends to transform to other polytypes. So, the possibility of SiC crytals growth was evaluated by different heating method. This study aimed to observe whether the resistant heating method would show stable growth and better quality of SiC single crystal than that of RF induction heating. As a result, polycrystalline SiC crystals were grown by the growth rate of 0.02~0.5 mm/hr under the condition of $2100{\sim}2300^{\circ}C$ at the bottom side of the crucible and 10~760 torr. The polycrystalline SiC crystals with 0.25 and 0.5 mm in thickness were grown successfully without seed and characterized by optical stereo microscopic observation.

Production of HCN, Weed Control Substance, by Pseudomonas koreensis and its Plant Growth-Promoting and Termiticidal Activities (Pseudomonas koreensis에 의한 잡초제어활성물질인 HCN 생성과 이 균주의 식물성장 촉진 및 흰개미 살충 활성)

  • Yoo, Ji-Yeon;Jang, Eun-Jin;Park, Soo-Yeun;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.27 no.9
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    • pp.771-780
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    • 2018
  • To develope a microbial weed control agent, HCN-producing bacteria were isolated, and their characteristics were investigated. A selected strain of WA15 was identified as Pseudomonas koreensis by morphological, cultural, biochemical and 16S rRNA gene analyses. The conditions for HCN production was investigated by a One-Variable-at-a-Time (OVT) method. The optimal HCN production conditions were tryptone 1%, glycine 0.06%, NaCl 1%, and an initial pH and temperature of 5.0 and $30^{\circ}C$, respectively. The major component for HCN production was glycine. Under optimal conditions, HCN production was about 3 times higher than that of the basal medium. The WA15 strain had physiological activities, such as indoleacetic acid that was associated with the elongation of plant roots and siderophore and ammonification inhibiting fungal growth, and produced hydrolytic enzymes, such as cellulase, pectinase and lipase. The strain was able to inhibit the growth of phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum, by the synergistic action of volatile HCN and diffusible antimicrobial compounds. A microscopic observation of R. solani that was teated with the WA15 strain showed morphological abnormalities of fungal mycelia, which could explain the role of the antimicrobial metabolites that were produced by the WA15 strain. The volatile HCN produced by the WA15 strain was also found to have insecticidal activity against termites. Our results indicate that Pseudomonas koreensis WA15 can be applied as a microbial agent for weed control and also as a termite repellent. Furthermore, it could be applied as a microbial termiticidal agent to replace synthetic insecticides.

THE EFFECT OF $CO_2$ LASER IRRADIATION ON ENAMEL SURFACE AND THE BOND STRENGTH OF SEALANT MATERIAL (탄산가스 레이저 조사가 법랑질 표면구조와 치면열구 전색재의 결합강도에 미치는 효과)

  • Yun, Dong-Sik;Kim, Yong-Kee;Kim, Jong-Soo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.4
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    • pp.761-771
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    • 1998
  • In this study, attempt has been made to evaluate the effect of $CO_2$ laser irradiation on enamel surface structure and the bond strength of sealant material. Conventional acid etching was used as a control technic for comparison. The results obtained from this experiment were as follows; 1. The highest mean shear bond strength value was observed in samples of Group I (acid-etching) with the statistical significance(p<.05) between all the other groups. 2. The shear bond strength in Group IV was the lowest among laser etching groups. but there were no significant difference between them(p>.05). 3. Scanning electron microscopic observation showed that the rough and irregular surface was created by $CO_2$ laser treatment with the formation of numerous pores, micro-cracks, and small bubble-like inclusion. Increasing the energy density induced localized surface melt with a thin smooth glaze-like appearance. 4. In acid-etched control specimen cohesive failure predominated, whereas adhesive failure was the main mode in laser-treated group. Based upon the above-mentioned results, it can be assumed that the $CO_2$ laser is not an adequate substitute for the acid-etch technique in enamel preconditioning. More studies are required to explore the effective condition of laser irradiation which could attain the better bond strength of restorative materials.

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A literature review on clinical/ laboratory misfit evaluation on implant-prosthesis (임플란트와 상부보철물의 임상적/실험적 부적합 평가에 관한 문헌고찰)

  • Kim, Jong-Hoi;Cho, Woong-Rae;Ko, Kyung-Ho;Huh, Yoon-Hyuk;Park, Chan-Jin;Cho, Lee-Ra
    • The Journal of the Korean dental association
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    • v.56 no.9
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    • pp.462-478
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    • 2018
  • The presence of implant-prosthesis misfits can cause various complications. It is very important to detect misfits to prevent such complications. There are various evaluation methods for misfit assessment including clinical methods and laboratory in vitro methods. The clinical misfit evaluation includes radiographic analysis, visual observation, probing, Sheffield test, evaluation with disclosing materials, and screw resistance test. The laboratory in vitro evaluation method includes indirect modelling evaluation and direct metrological visualization. Of the indirect modelling methods, photoelastic stress analysis, finite element analysis, strain gauge analysis, and microbial colonization analysis were reviewed. Of the direct metrological visualization, microscopic analysis, 3-D photogrammetric analysis, coordinate measuring analysis, and radiographic analysis were reviewed. In this review, the characteristics, advantages and disadvantages of each method were evaluated.

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ACUTE RESPONSE OF THE RAT INCISOR BY SINGLE AND FRACTIONATED IRRADIATION (단일 및 분할 방사선조사에 의한 백서절치의 급성반응에 관한 연구)

  • Rhee In-Suk;Park Tae-Won;Ahn Hyung-Kyu
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.19 no.1
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    • pp.39-48
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    • 1989
  • Six to eight-month-old female albino rats were used as experimental animals. As an irradiation equipment, a Co-60 was used. The experimental animals were divided to; 6 of the control group, 12 of the 500cGy single irradiation group, 12 of the 1000cGy fractionated irradiation group, and 12 of the 1500cGy fractionated irradiation group. From the first week to the forth, 3 rats were picked from each group every week to be sacrificed and fixed with formalin. Those rats were observed by means of H-E stain after being taken radiograph and decalcified. The analysis of radiographic findings and light microscopic findings gives results as follows: 1. The delay of dental eruption rate was found in every group which underwent the irradiation experiment. Dentin niche, osteodentin, and dentin island were formed in the parts which were damaged by the irradiation. 2. The longer the observation period was, the more deposit of osteodentin and dentin island was formed. 3. In the single irradiation group, the damage effect was in proportion to the increase of radiation dose, whereas the damage was much less in the fractionated group receiving the same dose. 4. The 500cGy single irradiation group got temporary repairable damage, while the 1000cGy single irradiation group got considerable damage and showed much slower eruption rate than the 500cGy single irradiation group. The basal portion of the 1500cGy single irradiation group, whose growth was arrested, was destroyed. 5. The fractionated group were irradiated 500cGy everyweek. Repair was visible during the interval periods. The damage was accumulated as irradiation repeated, but degree of damage was lower than that of the 1000cGy and 1500cGy single irradiation group.

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The effect of Er:YAG laser irradiation on the surface microstructure and roughness of hydroxyapatite-coated implant

  • Kim, Seong-Won;Kwon, Young-Hyuk;Chung, Jong-Hyuk;Shin, Seung-Il;Herr, Yeek
    • Journal of Periodontal and Implant Science
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    • v.40 no.6
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    • pp.276-282
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    • 2010
  • Purpose: The present study was performed to evaluate the effect of erbium:yttrium-aluminium-garnet (Er:YAG) laser irradiation on the change of hydroxyapatite (HA)-coated implant surface microstructure according to the laser energy and the application time. Methods: The implant surface was irradiated by Er:YAG laser under combination condition using the laser energy of 100 mJ/pulse, 140 mJ/pulse and 180 mJ/pulse and application time of 1 minute, 1.5 minutes and 2 minutes. The specimens were examined by surface roughness evaluation and scanning electron microscopic observation. Results: In scanning electron microscope, HA-coated implant surface was not altered by Er:YAG laser irradiation under experimental condition on 100 mJ/pulse, 1 minute. Local areas with surface melting and cracks were founded on 100 mJ/pulse, 1.5 minutes and 2 minutes. One hundred forty mJ/pulse and 180 mJ/pulse group had surface melting and peeling area of HA particles, which condition was more severe depending on the increase of application time. Under all experimental condition, the difference of surface roughness value on implant surface was not statistically significant. Conclusions: Er:YAG laser on HA-coated implant surface is recommended to be irradiated below 100 mJ/pulse, 1 minute for detoxification of implant surface without surface alteration.

A Study on the Micro-deformation of Plain Weave Carbon/Epoxy Composite-Polymer Foam Sandwich Structures during Curing (평직 탄소섬유 복합재료-고분자 포움 샌드위치 구조의 성형 중 미소변형에 관한 연구)

  • Kim Yong-Soo;Chang Seung-Hwan
    • Composites Research
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    • v.17 no.6
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    • pp.28-36
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    • 2004
  • Micro-tow deformation during forming of PVC foam-fabric composite sandwich structure is investigated to find out the correlation between forming condition and material deformation. The foams used in this research are PVC foams which have 4 different densities and the fabric composite is Carbon/epoxy prepreg which is plain weave (3k) as a skin material. Tow parameters such as crimp angle and tow amplitude are measured using microscope and a proper image tool and are compared with each other. In order to find out the effect of foam deformation during forming on tow deformation the compressive tests of foams are performed in three different environmental temperatures ($25^{\circ}C$, $80{\circ}C$, $125^{\circ}C$). The microscopic observation results show that the micro tow deformations are quite different from each other with respect to the foam density and forming pressure.

Electron Microscopic Alterations in Pediculus humanus capitis Exposed to Some Pediculicidal Plant Extracts

  • El Akkad, Dina M.H.;El-Gebaly, Naglaa Saad M.;Yousof, Hebat-Allah Salah A.;Ismail, Mousa A.M.
    • Parasites, Hosts and Diseases
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    • v.54 no.4
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    • pp.527-532
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    • 2016
  • Head lice, Pediculus humanus capitis, infestation is an important public health problem in Egypt. Inadequate application of topical pediculicides and the increasing resistance to the commonly used pediculicides made the urgent need for the development of new agents able to induce irreversible changes in the exposed lice leading to their mortality. The aim of the present work is to evaluate pediculicidal efficacy of some natural products such as olive oil, tea tree oil, lemon juice, and ivermectin separately in comparison with tetramethrin-piperonyl butoxide (licid), as a standard pediculicide commonly used in Egypt. The effects of these products were evaluated by direct observation using dissecting and scanning electron microscopes (SEM). Results showed that after 1 hr exposure time in vitro, absolute (100%) mortalities were recorded after exposure to 1% ivermectin and fresh concentrate lemon juice. The mortalities were decreased to 96.7% after exposure to tea tree oil. Very low percentage of mortality (23.3%) was recorded after 1 hr of exposure to extra virgin olive oil. On the other hand, the reference pediculicide (licid) revealed only mortality rate of 93.3%. On the contrary, no mortalities were recorded in the control group exposed to distilled water. By SEM examination, control lice preserved outer smooth architecture, eyes, antenna, respiratory spiracles, sensory hairs, and legs with hook-like claws. In contrast, dead lice which had been exposed to pediculicidal products showed damage of outer smooth architecture, sensory hairs, respiratory spiracles and/or clinching claws according to pediculicidal products used.

Detection of Acoustic Signal Emitted during Corrosion of 304 Stainless Steel (304 스테인레스 강의 부식 손상 중 발생하는 음향방출신호 분석)

  • Wu, Kaige;Choe, Chan-Yang;Byeon, Jai-Won
    • Journal of the Korean Society for Nondestructive Testing
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    • v.33 no.5
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    • pp.409-414
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    • 2013
  • In this work, corrosion of 304 stainless steel was evaluated by using acoustic emission(AE) technique. AE measurement system was set for detecting acoustic signal during accelerated corrosion test of the specimen. AE signal started to be detected after the time of pitting corrosion initiation was evaluated by anodic polarization curve. Pitting corrosion damage was confirmed by optical microscopic observation of the surface morphology. AE cumulative counts and amplitude according to corrosion time could be divided into three stages. These trends were discussed in relation with changing pitting corrosion mechanism. Feasibilities of AE technique for evaluation of corrosion damage and mechanism were suggested.

Cytokinetic Study of MCF-7 Cells Treated with Commercial and Recombinant Bromelain

  • Fouz, Nour;Amid, Azura;Hashim, Yumi Zuhanis Has-Yun
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.11
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    • pp.6709-6714
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    • 2013
  • Background: Breast cancer is a leading cause of death in women. The available chemotherapy drugs have been associated with many side effects. Bromelain has novel medicinal qualities including anti-inflammatory, anti-thrombotic, fibrinolytic and anti-cancer functions. Commercially available bromelain is obtained through tedious methods; therefore, recombinant bromelain may provide a cheaper and simpler choice with similar quality. Materials and Methods: This study aimed to assess the effects of commercial and recombinant bromelain on the cytokinetic behavior of MCF-7 breast cancer cells and their potential as therapeutic alternatives in cancer treatment. Cytotoxic activities of commercial and recombinant bromelain were determined using (sulforhodamine) SRB assay. Next, cell viability assays were conducted to determine effects of commercial and recombinant bromelain on MCF-7 cell cytokinetic behavior. Finally, the established growth kinetic data were used to modify a model that predicts the effects of commercial and recombinant bromelain on MCF-7 cells. Results: Commercial and recombinant bromelain exerted strong effects towards decreasing the cell viability of MCF-7 cells with $IC_{50}$ values of 5.13 ${\mu}g/mL$ and 6.25 ${\mu}g/mL$, respectively, compared to taxol with an $IC_{50}$ value of 0.063 ${\mu}g/mL$. The present results indicate that commercial and recombinant bromelain both have anti-proliferative activity, reduced the number of cell generations from 3.92 to 2.81 for commercial bromelain and to 2.86 for recombinant bromelain, while with taxol reduction was to 3.12. Microscopic observation of bromelain-treated MCF-7 cells demonstrated detachment. Inhibition activity was verified with growth rates decreased dynamically from 0.009 $h^{-1}$ to 0.0059 $h^{-1}$ for commercial bromelain and to 0.0063 $h^{-1}$ for recombinant bromelain. Conclusions: Commercial and recombinant bromelain both affect cytokinetics of MCF-7 cells by decreasing cell viability, demonstrating similar strength to taxol.