• 한국방사선학회논문지
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• 제13권4호
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• pp.623-628
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• 2019

본 연구의 목적은 시간 분해능이 향상된 비지역적 평균 (fast non local means, FNLM) 노이즈 제거 알고리즘을 모델링하여 광학 현미경 영상에서의 적용 가능성을 확인하는 것이다. 이를 위해 실제 흰쥐 (mouse)의 첫째어금니 치아를 사용하여 영상을 획득한 후 기존에 널리 사용되고 있는 노이즈 제거 알고리즘과 제안하는 FNLM 알고리즘을 각각 적용하여 비교하였다. 정량적 평가는 대조도 대 잡음비 (contrast to noise ratio, CNR), 변동계수 (coefficient of variation, COV), 그리고 최근에 개발된 no reference 기반의 방법인 natural image quality evaluator (NIQE)와 Blind/referenceless image spatial quality evaluator (BRISQUE)를 사용하였다. 결과적으로 모든 정량적 평가 인자에서 제안하는 FNLM 노이즈 제거 알고리즘이 가장 우수한 값을 나타내었다. 특히나 치아의 전체적인 형태학적 영상을 분석할 수 있는 NIQE와 BRISQUE 인자는 원본영상에 비하여 각각 1.14와 1.12배 향상됨을 확인할 수 있었다. 결론적으로 소동물 치아 광학 현미경 영상에서의 FNLM 노이즈 제거 알고리즘의 유용성 및 가능성을 증명하였다.

• Applied Microscopy
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• 제37권3호
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• pp.199-208
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• 2007

본 장치는 실험자가 레이저 빔과 광학 렌즈를 조정하여 이미지 및 회절도형의 형성, 보강 및 소멸간섭과 같은 파동광학 현상을 이해하도록 제작된 실험 장치이다. 실험장치는 광원으로 쓰이는 레이저빔과 빔의 광축을 정렬하는 광원 부분과 시료대, 대물렌즈, 중간렌즈, 확대렌즈, CCD system, 컴퓨터, 그리고 렌즈를 상하 조절하는 경통부분으로 구성된다. 본 장치를 통해서 다양한 회절격자의 이미지 및 회절도형을 최대 약 44배 확대할 수 있고, 최대 약 5um의 분해능을 가지고 분석할 수 있다. 이 장치는 전자현미경 이용자들이 TEM의 원리를 보다 쉽게 이해하는데 도움을 주리라 기대한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 심포지엄 논문집 정보 및 제어부문
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• pp.111-112
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• 2008

In this study, an image processing algorithm with Lab VIEW IMAQ Module is presented and discussed for the development of a microscopic gap measuring system using CCD sensor. LabVIEW IMAQ development application that is developed by National Instrument Co. Inc. has a graphical interface and many image processing functions, so that it provides an easier environment for the development of a measurement algorithm using the image data. Actual microscopic gap measuring experiment was executed using the algorithm developed in this study and it showed a promising result. Also, we could obtain the convenience of Lab VIEW IMAQ in developing the algorithm.

• Corrosion Science and Technology
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• 제15권6호
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• pp.265-270
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• 2016

The ellipso-microscopic observation of a titanium surface undergoing anodization in $0.05mol\;dm^{-3}$ of $H_2SO_4$ was conducted. During irradiation by ultra-violet (UV) light with a wavelength of 325 nm, the titanium surface allowed for the flow of a photo-induced current and showed up as a bright, patch-like image on an ellipso-microscopic view. The brightness and patch-pattern in the image changed with flowing photo-induced current. The changes in the brightness and the image corresponded to the formation and/or degradation of titanium oxide due to the photo-electrochemical reaction of the oxide. An in situ monitoring using the ellipso-microscope revealed that the film change was dependent on the irradiation light power, by UV-light increases the anodic current and results in the initiation of pitting at lower potentials as compared with the non-irradiated condition.

• 한국통신학회논문지
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• 제34권11B호
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• pp.1311-1319
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• 2009

집적 영상 현미경에서 획득한 요소 영상을 이용하여 집적 영상 디스플레이에서 재생하였다. 일반화된 서로 다른 두 렌즈 어레이를 이용하는 집적 영상의 획득과 재생의 관계를 이용하여 집적 영상 현미경 및 디스플레이 시스템에 적용하였다. 집적 영상 현미경에서 획득한 요소 영상을 재생하기 위해서 스케일링이 적용된 요소 영상이 필요하다. 집적 영상을 재생할 때 요소 영상의 정보 손실을 최소화하는 스케일링 계수와 요소 영상의 왜곡을 최소화하는 스케일링 계수를 선택해야 한다. 본 연구에서는 $125{\mu}m$ 피치 사이즈의 마이크로 렌즈 어레이를 가지는 집적 영상 현미경으로 시료를 촬영하고 1mm 피치 사이즈의 렌즈 어레이를 가지는 집적 영상 디스플레이로 촬영된 시료를 3차원 영상으로 재생하는 실험을 수행하였다. 이 때, 요소 영상의 스케일링 계수는 집적 영상 현미경에서 획득한 요소 영상의 정보 손실을 최소화 하는 값으로 설정하였다.

• Journal of Multimedia Information System
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• 제1권1호
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• pp.87-93
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• 2014

The aim of microscopic image restoration is to recover the image by applying the inverse process of degradation, and the results facilitate automated and improved analysis of the image. In this work, we consider the problem of image restoration as a minimization problem of convex cost function, which consists of a least-squares fitting term and regularization terms with non-negative constraints. The finite step method is proposed to solve this constrained convex optimization problem. We demonstrate the convergence of this method. Efficiency and restoration capability of the proposed method were tested and illustrated through numerical experiments.

• 대한의용생체공학회:의공학회지
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• 제44권3호
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• pp.176-181
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• 2023

During cancer treatment, the patient's response to drugs appears differently at the cellular level. In this paper, an image-based cell phenotypic feature quantification and key feature selection method are presented to predict the response of patient-derived cancer cells to a specific drug. In order to analyze the viability characteristics of cancer cells, high-definition microscope images in which cell nuclei are fluorescently stained are used, and individual-level cell analysis is performed. To this end, first, image stitching is performed for analysis of the same environment in units of the well plates, and uneven brightness due to the effects of illumination is adjusted based on the histogram. In order to automatically segment only the cell nucleus region, which is the region of interest, from the improved image, a superpixel-based segmentation technique is applied using the fluorescence expression level and morphological information. After extracting 242 types of features from the image through the segmented cell region information, only the features related to cell viability are selected through the ReliefF algorithm. The proposed method can be applied to cell image-based phenotypic screening to determine a patient's response to a drug.

• ALGAE
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• 제19권2호
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• pp.79-90
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• 2004

Nuclear DNA contents of spermatia from eight ceramiacean and four dasyacean algae (Ceramiales, Rhodophyta) and microspores from two land plants were estimated by fluorescence microscopic image processing and their nuclear SSU rDNA sequence data were analyzed. In frequency distribution patterns, the DAPI-stained nuclear volume (NV) of spermatia showed two peaks corresponding to 1C and 2C. Nuclear 2C DNA contents estimated from NV were 0.45-2.31 pg in ceramiacean and 0.40-0.57 pg in dasyacean algae and 8.42-9.51 pg in two land plants, Capsicum annuum and Nicotiana tabacum. By nuclear patterning of vegetative cells derived from an apical cell, 2C DNA contents of spermatia were 2.31 pg in an alga having uninucleate and non-polyploid nucleus (Aglaothamnion callophyllidicola), 0.45-1.94 pg in algae having uninucleate and polyploid nucleus (Antithamnion spp. and Pterothamnion yezoense), and 0.40-0.62 pg in algae having multinucleate and non-polyploid nuclei (Griffithsia japonica and dasyacean algae). Each mature spermatium and microspore (pollen grain) seemed to have a 2C nucleus, which may provide a genetic buffering system to protect the genetic content of a spermatium and microspore from potentially lethal mutations. Nuclear DNA content and SSU rDNA sequence of Antithamnion sparsum from Korea were reasonably different from those of Antithamnion densum from France. The data did not support the previous taxonomic studies that these two taxa could be conspecific.

• Applied Microscopy
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• 제44권4호
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• pp.138-143
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• 2014

Transmission electron microscope (TEM) is an excellent tool for studying the structure and properties of nanostructured materials. As the development of $C_s$-corrected TEM, the direct analysis of atomic structures of nanostructured materials can be performed in the high-resolution transmission electron microscopy (HRTEM). Especially, fast Fourier transform (FFT) technique in image processing is very useful way to determine the crystal structure of HRTEM images in reciprocal space. To apply FFT technique in HRTEM analysis in more reasonable and friendly manner, we made a new circular region of interest (C-ROI) FFT script and tested it for several HRTEM analysis. Consequentially, it was proved that the new FFT application shows more quantitative and clearer results than conventional FFT script by removing the streaky artifacts in FFT pattern images. Finally, it is expected that the new FFT script gives great advantages for quantitative interpretation of HRTEM images of many nanostructured materials.

• 한국축산시설환경학회지
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• 제7권2호
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• pp.103-110
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• 2001

This study conducted to monitor decomposition process of the charomatophore pollution solution of an animal slurry by using a CCD camera. After the solution was put into test tube, the images(R, G, B, H, L, S) values of the solution were measured by the imgae processing system, and those of it\`s optical density were measured for three hours to be decomposed by microscopic organism. The values of measured for three hours to be decomposed by microscopic organism. The values of measured images(R, G, B, H, L, S) were analysed and compared with those of the optical density. Some of the results are as follows. 1. High correlation coefficients, which analyzed by using data on linear equations, were 0.9557 and 0.9672. They were decreased regularly in this R-value experiment of RGB level. The microscopic organism in this experiment was effective for decomposition of the red charomatophore pollution solution. 2. The values of all correlation coefficients from relationship between RGB-value and optical density were more than 0.95 except H-values. RGB-values, which were average values of summed R, G, B values, had correlation coefficients of 0.9863, 0.9937. These results showed so good relationship that decomposition process of charomatophore pollution solution could be monitored by a image processing system.