• Title/Summary/Keyword: Microscopic examination

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Prevalence of Fungal Infection in Diabetic Foot Ulcer (당뇨족 궤양에서 진균감염의 유병률)

  • Yoo, Yeon Sik;Han, Seung-Kyu;Kim, Woo Kyung
    • Archives of Plastic Surgery
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    • v.35 no.2
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    • pp.134-138
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    • 2008
  • Purpose: In a diabetic foot ulcer, superficial fungal infection of the foot may disrupt the skin integrity and provide an avenue for bacterial infection. The prevalence of fungal infection in diabetic foot ulcer has been reported as 12-30% in Caucasian patients. However, no data are available for Korean patients. The purpose of the study was to examine the prevalence of fungal infection in diabetic foot patients admitted in our clinic. Methods: This study included consecutive 30 diabetic foot ulcer patients(24 males and 6 females) admitted from May 2007 to July 2007. The mean age was 60.5 years. All patients underwent mycological examination including direct microscopic examination in KOH and culturing on slants and tubes with Sabouraud's glucose agar. Results: Clinical signs of presumed fungal infection, which were examined by dermatologists, were found in all patients included in this study. Direct microscopic examination was positive for the specimens of the skin and the toenails in 28(93.3%) and 25(83.3%) patients, respectively. In addition, culture result was positive for the specimens of the skin and the toenails in 5(16.73%) and 7(23.3%) patients, respectively. Conclusion: The prevalence of fungal infection in diabetic foot ulcer patients admitted in our clinic is much higher than that in Caucasian patients(83.3-93.3% vs 12-30%).

AN EXPERIMENTAL STUDY ON THE EFFECT OF SODIUM β-GLYCEROPHOSPHATE UPON THE FORMATION OF CALCIFIED TISSUE IN THE EXPOSED DOGS' DENTAL PULP (Sodium β-glycerophosphate가 치수조직의 경조직 형성에 미치는 영향에 관한 연구)

  • Lee, In-Sook;Lee, Chung-Sik
    • Restorative Dentistry and Endodontics
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    • v.14 no.2
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    • pp.77-97
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    • 1989
  • The purpose of this study was to observe the effect of ${\beta}GP$ in the remaining dental pulp tissue after pulpotomy in the dogs' teeth. For vital pulpotomy, 72 dogs' teeth were used and class V cavities were prepared and the pulps were amputated. ZOE and Dycal (Caulk Co., USA) were placed over the amputated tissue and cavities were sealed with ZOE cement in the control group. In the experimental group, ${\beta}GP$, ${\beta}GP$-ZOE, ${\beta}GP$-Dycal were placed over the exposed pulp tissues respectively. Dogs were sacrificed after 1, 2 and 4 weeks following the operations and the teeth were decalcified in the nitric acid, sectioned and stained with HE for light microscopic examination. For electron microscopic examination, specimens were made after 2 and 4 weeks following the operation. A comparative microscopic examination revealed as follows. 1. The dentin bridge was formed continuously due to osteodentin in the ${\beta}GP$-Dycal group at the 2nd week, the dentin bridge composed of osteodentin and tubular dentin was observed at the 4th week. 2. Osteodentin formation was vigorously in the ${\beta}GP$-Dycal than in the Dycal group. 3. In the surface of osteodentin the osteodentinoblasts showing vivid synthetic activity were observed and the matrix vesicles were presented during calcification of osteoid dentin matrix. 4. The dentin bridge formation was not observed in ${\beta}GP$ group and ${\beta}GP$-ZOE group.

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HEALING PATTERN OF BONE REGERNERATION IN PERIIMPLANT SPACE AFTER IMMEDIATE IMPLANT PLACEMENT;AN EXPERIMENTAL STUDY IN DOGS (발치후 즉시 임프란트 식립시 임프란트 주위공간의 치유양상에 관한 실험적 연구)

  • Choi, Mee-Sook;Kim, Jong-Eon;Kang, Bo-Won;Kim, Sung-Moon;Rim, Jae-Suk;Kwon, Jong-Jin
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.16 no.4
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    • pp.499-507
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    • 1994
  • The aim of this experiment is to compare the healing process of extraction sockets after immediate implant placement with those using autogenous bone grafts and guided tissue regeneration with Gore-Tex. The first lower premolars and the second premolars of six experimental dogs were extracted and Stryker fin type implants were placed into the extraction sockets immediately after extraction. In the control group, any graft materials were'not used and the dead space around implants was left in itself and covered with only periosteum. In the experimental group A, implants were covered with Gore tex without any bone grafts, and in the experimental group B, the dead space around implants was filled with the bone chips gained from drilling procedure. Each experimental dogs were sacrificed at the 1st, 2nd, 3rd, 6th, and 8th week and the specimens were observed by gross examination, radiological examination, and light microscopic examination. The following results were obtained. 1. Well healed soft tissue and no mobility of the implants were observed in control and two experimental groups. 2. In the radiogical examination, radiopacity around implants had been increased gradually. 3. In the microscopic examination, there were good healing process and active new bone formation in both in the experimental groups, Especially the more amount of new bone formation occurred in the experimental group B using bone chips. 4. Bone chip grafts and guided tissue regeneration (GTR) using Gore-Tex may be one of the successful methods in the immediate implantation.

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Microscopic Analysis of Early Age Cement Paste Axially Compressed 20% (20% 축압축 시킨 초기재령 시멘트 페이스트 미세 분석)

  • 김성훈;김동완;양종석;김장호
    • Proceedings of the Korea Concrete Institute Conference
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    • 2002.05a
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    • pp.189-194
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    • 2002
  • Many researches in the past have shown that a majority initial cracking in concrete are caused during early age period. Therefore, the close examination of early age concrete behavior under various stress conditions is necessary to fully understand the cracking mechanism of concrete. In this study early age cement paste specimen is axially strained up to 20% of its original length by laterally reinforcing it. This type of test is called "Tube Squash Test" and has been previously used to apply up to 50% axial strain on concrete. Microscopic analyses (XRD, FESEM, EDS and DSE/TG) are performed on 20% axially strained early age cement paste specimen. The analysis results show that the microscopic structures and material characteristics of 20% axially strained cement paste remained same as the unstrained cement paste.

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The Clinical Use of Routine Urinalysis (기본 요분석검사의 임상적 이용)

  • Lee, Seung Joo
    • Childhood Kidney Diseases
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    • v.17 no.2
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    • pp.35-41
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    • 2013
  • Routine urinalysis is a simple, economical, and useful test that facilitates the detection of urinary system diseases and monitoring of renal disease progression. It consists of 4 parts of specimen evaluation, gross examination, a dipstick urinalysis, and a sediment microscopic urinalysis. Urine specimens should first be evaluated in terms of acceptability, and thereafter, the gross appearance is examined for color, turbidity, and odor. In particular, a dipstick urinalysis is an easy and rapid test that provides information on the multiple physicochemical properties of the urine sample. Moreover, although a sediment microscopic urinalysis is time-consuming, it provides information on the cells, microorganisms, casts, and crystals. In the present report, the clinical significance of the routine urinalysis and the problems concerning interpretation are summarized.

Otitis Externa caused by Malassezia furfur in a Miniature Pig (애완 미니 돼지의 Malassezia furfur에 의한 외이도염 증례)

  • Han, Jae-Ik;Na, Ki-Jeong
    • Journal of Veterinary Clinics
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    • v.26 no.3
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    • pp.303-305
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    • 2009
  • A 7-month-old, female miniature pig was presented with excessive cerumen and pruritus. Greasy brown cerumen in both exteranal ear canal and sporadic head shaking were observed in the physical examination. Numerous budding yeasts in the cerumen were examined on microscopic examination. For species identification, PCR-RFLP using incubated colony on modified Dixon's medium was performed and finally, causative yeast was identified as M. furfur.

A Novel Hyperspectral Microscopic Imaging System for Evaluating Fresh Degree of Pork

  • Xu, Yi;Chen, Quansheng;Liu, Yan;Sun, Xin;Huang, Qiping;Ouyang, Qin;Zhao, Jiewen
    • Food Science of Animal Resources
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    • v.38 no.2
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    • pp.362-375
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    • 2018
  • This study proposed a rapid microscopic examination method for pork freshness evaluation by using the self-assembled hyperspectral microscopic imaging (HMI) system with the help of feature extraction algorithm and pattern recognition methods. Pork samples were stored for different days ranging from 0 to 5 days and the freshness of samples was divided into three levels which were determined by total volatile basic nitrogen (TVB-N) content. Meanwhile, hyperspectral microscopic images of samples were acquired by HMI system and processed by the following steps for the further analysis. Firstly, characteristic hyperspectral microscopic images were extracted by using principal component analysis (PCA) and then texture features were selected based on the gray level co-occurrence matrix (GLCM). Next, features data were reduced dimensionality by fisher discriminant analysis (FDA) for further building classification model. Finally, compared with linear discriminant analysis (LDA) model and support vector machine (SVM) model, good back propagation artificial neural network (BP-ANN) model obtained the best freshness classification with a 100 % accuracy rating based on the extracted data. The results confirm that the fabricated HMI system combined with multivariate algorithms has ability to evaluate the fresh degree of pork accurately in the microscopic level, which plays an important role in animal food quality control.

Light and Electron Microscopic Characterization of Husk from Korean Rice

  • Adya P. Singh;Park, Byung-Dae;Wi, Seung-Gon;Lee, Kwang-Ho;Yoon, Tae-Ho;Kim, Yoon-Soo
    • Plant Resources
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    • v.5 no.2
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    • pp.95-103
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    • 2002
  • Microscopic techniques were used to observe the microstructure of rice husk. Microscopic examination showed that two main components of husk, lemma and palea consisted of outer epidermis, layers of fibers, vascular bundles, parenchyma cells, and inner epidermis, in sequence from the outer to the inner surface. The outer epidermal walls were extremely thick, highly convoluted and lignified. The underlying fibers were also thick-walled and lignified. Parenchyma cells were thin-walled and unlignified. Inner epidermal cells were also unlignified. The outer surface of both lemma and palea were conspicuously ridged, but the lower surface had a flat appearance. As part of a detailed study to characterize rice husk using microscopic and micro-analytical techniques, distribution of silica was also examined, and is presented elsewhere. Rice husk can potentially be used as a raw material for making composite products and the observations presented here form valuable background information for our future work related to product development.

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Description of Microscopic Morphology of Leptochiton hakodatensis (Mollusca: Polyplacophora)

  • Park, Jina;Lee, Yucheol;Kim, Yukyung;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.1
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    • pp.14-19
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    • 2022
  • Leptochiton Gray, 1847 is one of the most ancient chiton groups which includes more than 130 species that occur in cold and deep waters worldwide. Due to their small-sized body, they are often confused as juveniles of other chiton species. Moreover, lack of morphological information makes species identification of this group very challenging. To date, only two Leptochiton species(L. fuliginatus and L. rugatus) have been reported from Korean waters. In this study, we found L. hakodatensis(Thiele, 1909) for the first time in Korea and described microscopic morphological characters of valves (tegmentum sculpture), girdle scale, and radula using a scanning electron microscopy (SEM). Leptochiton hakodatensis is morphologically similar to L. fuliginatus and L. rugatus, but differently characterized by having dorso-ventrally rounded (not carinated) intermediate valves, girdle (perinotum) scales sculptured with 4-7 longitudinal ribs, and bicuspid major lateral teeth of radula. In addition to morphological examination, we determined the partial mitochondrial cytochrome c oxidase subunit I(cox1) as a DNA barcode sequence information. This is the first report that describes microscopic characters (tegmentum of valves, girdle structure, and radula) of L. hakodatensis using a SEM. This study provides a morphological basis for describing Leptochiton species and discovery of a "hidden" species of this genus.

Characteristics of Fatigue in Sasang Constitution by Analyzing Questionnaire and Medical Devices Data (설문지와 의료기기 자료 분석을 통한 사상체질별 피로 특성 연구)

  • Kim, Koo;Ha, Ye-Jin;Park, Soo-Jeong;Choi, Na-Rae;Lee, Young-Seop;Joo, Jong-Cheon
    • Journal of Sasang Constitutional Medicine
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    • v.25 no.4
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    • pp.306-319
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    • 2013
  • Objectives The purpose of this study was to find correlations between gastrointestinal disorders, pain, sleep disorder, fatigue, and to figure out characteristics of fatigue in Sasang Constitution using medical devices data. Methods Sixty three subjects were divided into fatigue group and non-fatigue group, both groups had to undergo blood tests, questionnaire, Sasang constitutional analysis tool (SCAT), pulse wave analyzer examination, heart rate variability examination, nail fold capillary microscopic examination. Results 1) The results of questionnaire about fatigue, gastrointestinal disorder, pain, sleep disorder, quality of life had significant differences between fatigue and non-fatigue groups. 2) Soeumin had more serious gastrointestinal problem and Soyangin had more serious sleep disorder in fatigue groups than non-fatigue groups. 3) According to blood test results, there was no difference between fatigue and non-fatigue groups. 4) Elastic parameter of pulse wave analyzer and nail fold capillary microscopic examination showed significant differences between fatigue and non-fatigue groups in Soyangin. Conclusions We reach the conclusion that fatigue is usually accompanied by gastrointestinal disorder, pain, sleep disorder, deterioration in the quality of life. In Soeumin, treating gastrointestinal disorders can be helpful for treatment of fatigue. In Soyangin, improving sleep disorder may be more effective way to treat fatigue.