• Title/Summary/Keyword: Microscopic examination

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Antibacterial property of 4-hexylresorcinol incorporated in silk mat after sterilization

  • Kim, Seong-Gon;Kweon, HaeYong;Jo, You-Young
    • International Journal of Industrial Entomology and Biomaterials
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    • v.42 no.2
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    • pp.46-50
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    • 2021
  • When 4-hexylresorcinol (4HR) incorporated silk mat is used for clinical application, sterilization process is essential. In this study, 4HR incorporated silk mat was sterilized with ethylene oxide (EO) gas or autoclave, and then antibacterial property was evaluated. For the examination of micro-structure after the sterilization, scanning electron microscopic (SEM) exam and Fourier Transform Infrared (FT-IR) spectroscopy were also done. There was no gross difference after sterilization in SEM exam and FT-IR spectra. Antibacterial property of 4HR was also maintained after the sterilization. In conclusion, EO gas or autoclave did not damage the antibacterial property of 4HR in the silk mat.

Death due to Aspergillus Tracheobronchitis: An Autopsy Case (아스페르길루스 기관기관지염에 의한 사망: 부검 1예)

  • You, Tack Kune;Choi, Byung Ha;Lee, Bong Woo;Choi, Young Shik
    • The Korean Journal of Legal Medicine
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    • v.42 no.4
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    • pp.164-167
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    • 2018
  • Aspergillus infection is the most common cause of death due to fungi in immunocompromised hosts. Aspergillus tracheobronchitis is an uncommon but severe form of invasive pulmonary aspergillosis, which is limited entirely or mainly to the tracheobronchial tree and can often be life-threatening. We report a case of a 54-year-old man who died from Aspergillus tracheobronchitis without an underlying disease. Autopsy revealed an extensive yellowish plaque adhering to the trachea and bronchial wall. The microscopic examination of the trachea and bronchus revealed septate branching hyphae of Aspergillus.

Chemotherapy of Mammary Comedocarcinoma with Doxorubicin in a Dog

  • Kim, Yeonsoo;Seo, Kyoungwon;Song, Kunho
    • Journal of Veterinary Clinics
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    • v.38 no.6
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    • pp.274-278
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    • 2021
  • An 11-year-old, intact female, Miniature Poodle presented with an abdominal mass. The abdominal mass was located around the right fifth mammary gland. The surgically removed mammary mass was subjected to histopathological examination. Based on the microscopic interpretation, a final diagnosis of an intermediate grade infiltrative mammary comedocarcinoma was established. Computed tomography for metastasis evaluation after surgical resection of the tumor showed enlarged right medial iliac and right inguinal lymph nodes, and a micronodule in the accessory lung lobe, suggesting metastasis of the mammary gland tumor. Doxorubicin, a chemotherapeutic drug, was administered six times at three week intervals. However, despite chemotherapy, the masses around the fourth and fifth mammary gland on the right side enlarged in size, and the treatment was discontinued at the request of the owner. The anticancer response to mammary comedocarcinoma is poor and the patient is in hospice management. This is the first attempt to treat a case of canine mammary comedocarcinoma in South Korea.

Cultural characteristics survey of Clavicorona pyxidata (WANG GWAN) strains collected from Korea (좀나무싸리버섯(Clavicorona pyxidata) '왕관' 수집균주의 배양적 특성조사)

  • Chang, H.Y;Park, S.A.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.12 no.1
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    • pp.11-19
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    • 2010
  • This study was carried out to investigate the characteristics survey of Clavicorona pyxidata strains. C. pyxidata are selected 3 kinds of strains collected in GWANG DEOCK Mt., DO BONG Mt., and BUK HAN Mt., Mycelial growth of C. pyxidata collected from GWANG DEOCK and BUK HAN Mt. is 64mm, 60mm for 18days respectively. DO BONG Mt strain is 85mm for 9 days. Transparent crystal body of C. pyxidata mycelia were appeared by microscopic examination of 100 magnification. Optimal temperature of mycelial growth in C. pyxidata strains are 25~30℃ respectively.

THE EFFECT OF RIGID FIXATION ON THE FRONTONASAL SUTURE IN GROWING RABBITS (강성(剛性) 고정(固定)이 성장중(成長中)인 가토(家兎)의 전두비골봉합(前頭鼻骨封合)에 미치는 영향(影響)에 관(關)한 연구(硏究))

  • Kim, Hyo-Jong;Kim, Yeo-Gab
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.16 no.3
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    • pp.290-302
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    • 1994
  • This study was aimed to clarify the histopathological changes in the experimental animal model subjcted to rigid fixation performed across the frontonasal sutrue in growing rabbits. Sixteen rabbits aged 6 weeks used. In experimental group(n=12), rigid fixation with miniplates and screws was performed across the frontonasal suture. Control group(n=4) was those with periosteal elevation only. Experimental animals were sacrificed on the 2nd, 4th, 8th, and 12th week after operation, and frontonasal suture area was excised for light microscopic and scanning electron microscopic examination. The results obtained were as follows : 1. In control groups, collagen fiber bundles ran in the midportion of bone sutrue and cambial layers were seen at bone surface. Sutural surfaces are beveled and external and internal bony projected portions were observed. 2. In experimental groups, distance of bone suture was decreased by new bone formation on the 2nd week, while increased by bone resorption at the miniplate applied area and bone formation in the adjacent bone on the 4th week. 3. In experimental groups, the original bone surface was almost resorbed and new bone formation was found on the 8th week. Regulary-run collagen fibers, smooth and dense bone surfaces were similar to the bone patterns of control groups on the 12th week. Above results suggest that bone formation is restricted where the miniplate is applied, while compensatory growth is appeared in the adjacent bont. It is considered that rigid fixation with miniplates and acrews results in a little disturbance of sutural growth of the craniofacial bone in infancy and children when applied for short duration.

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Morphology and taxonomy of the Aphanizomenon spp. (Cyanophyceae) and related species in the Nakdong River, South Korea

  • Ryu, Hui Seong;Shin, Ra Young;Lee, Jung Ho
    • Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.144-152
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    • 2016
  • Background: The purpose of this study is to describe the morphological characteristics of the Aphanizomenon spp. and related species from the natural samples collected in the Nakdong River of South Korea. Results: Morphological characteristics in the four species classified into the genera Aphanizomenon Morren ex Bornet et Flahault 1888 and Cuspidothrix Rajaniemi et al. 2005 were observed by light microscopy. The following four taxa were identified: Aphanizomenon flos-aquae Ralfs ex Bornet et Flahault, Aphanizomenon klebahnii Elenkin ex Pechar, Aphanizomenon skujae $Kom{\acute{a}}rkov{\acute{a}}-Legnerov{\acute{a}}$ et Cronberg, and Cuspidothrix issatschenkoi ($Usa{\check{c}}ev$) Rajaniemi et al. Aph. flos-aquae and Aph. klebahnii always formed in fascicles; the others only occurred in solitary. Aph. flos-aquae was similar to Aph. klebahnii, whereas these species differed from each other by the size and shape of fascicles, which was macroscopic in Aph. flos-aquae and microscopic in the Aph. klebahnii. One of their characteristics was that trichomes are easily disintegrating during microscopic examination. C. issatschenkoi could be clearly distinguished from other species by hair-shaped terminal cell. Its terminal cell was almost hyaline and markedly pointed. Young populations of the species without heterocytes run a risk of a misidentification. Aph. skujae was characterized by akinete. Morphological variability of akinetes from natural samples collected in the Nakdong River was rather smaller than those reported by previous study. Conclusions: C. issatschenkoi are described for the first time in the Nakdong River. In addition, Aph. klebahnii and Aph. skujae are new to South Korea.

A Study on a Morphological Identification of Cnidium Rhizome and Ligusticum Rhizome (천궁(川芎)의 형태(形態)에 관한 연구(硏究))

  • Choi, Jeong-Kook;Lim, Deog-Bin;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.20 no.4
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    • pp.95-101
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    • 2005
  • Objectives : In order to distinguish Cnidii Rhizoma and Ligustici Chuanxiong Rhizoma (cultivated in Korea and China), their external and internal states were observed via microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : Cnidii Rhizoma, Ligustici Chuanxiong Rhizoma (from Korea), and Ligustici Chuanxiong Rhizoma (from China) all demonstrated similar patterns as following : 1. external form 1) showing irregular nodular form with $2{\sim}7cm$ diameter. 2) having several trochites in parallel, and nodulose root trace on surface. 3) in addition to yellow-white or yellow-gray intersections, yellow-brown oil sacs were distributed, with cambium resembling a form of annulus. 2. internal form 1) its root and rhizome is composed of 10 layers of cork, with narrow cortical layers. 2) its phloem is wide, and oval light yellow-brown oil sacs are distributed around. The closer to the cambium, the smaller it is. 3) cambium is a form of a wave-like ring or an irregular polygon. 4) xylem and vessel are rare, most of which are arranged in a 'V' format, and sometimes xylem fiber is found. 5) pith is composed of parenchymatous cell, and parenchyma is full of starch grains. Conclusions : In conclusion, C officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) have rhizomes in a similar form with no special difference. Therefore, it is concluded that C. officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) are categorized into the same species.

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A Case of Carcinoid-Type Tumorlets of The Lung (Carcinoid형 소종양 1예)

  • Park, Jae-Yong;Won, Jun-Hee;Lee, Yang-Ill;Han, Chun-Duk;Jung, Tae-Hoon;Chung, Jun-Mo;Kim, Duk-Sil;Jheon, Sang-Hoon;Lee, Seung-Cheol;Kwak, Jung-Sik
    • Tuberculosis and Respiratory Diseases
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    • v.42 no.3
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    • pp.370-374
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    • 1995
  • Carcinoid-type tumorlets of the lung are nodular microscopic proliferation of round and spindle-shaped small cells which originated from bronchial or bronchiolar Kulchitsky-type neuroendocrine cells, which are usually encountered as an incidental finding during microscopic examination of the lungs at autopsy or surgically removed for bronchiectasis or other reasons. We report one case of carcinoid-type tumorlets in the lung which was surgically removed from a patient who had bronchiectasis, and the cells of tumorlets showed immunohistochemical reactivities for markers of epithelial and neuroendocrine differentiation.

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Biomechanical Testing of Anterior Cervical Spine Implants: Evaluation of Changes in Strength Characteristics and Metal Fatigue Resulting from Minimal Bending and Cyclic Loading

  • Kim, Sung-Bum;Bak, Koang-Hum;Cheong, Jin-Hwan;Kim, Jae-Min;Kim, Choong-Hyun;Oh, Seong-Hoon
    • Journal of Korean Neurosurgical Society
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    • v.37 no.3
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    • pp.217-222
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    • 2005
  • Objective: To achieve optimal fit of implant, it is necessary to bend the implant during spine surgery. Bending procedure may decrease stiffness of plate especially made of titanium and stainless steel. Typically titanium suffers adverse effects including early crack propagation when it is bent. We investigate whether 6 degree bending of titanium plates would decrease the stiffness after full cyclic loading by comparing with non-bending titanium plates group. Methods: Authors experimented 40 titanium alloy plates of 57mm in length, manufactured by 5 different companies. Total 40 plates were divided into two groups (20 bent plates for experimental group and 20 non-bent plates for control group). Twenty plates of experimental group were bent to 6 degree with 3-point bending technique and verified with image analyzer. Using the electron microscope, we sought for a initial crack before and after 3-point bending. Mechanical testing by means of 6000 cyclic axial-compression loading of 35N in compression with moment arm of 35mm-1.1 Nm was conducted on each plate and followed by the electron microscopic examination to detect crack or fissure on plates. Results: The stiffness was decreased after 6000 cyclic loading, but there was no statistically significant difference in stiffness between experimental and control group. There was no evidence of change in grain structure on the electron microscopic magnification. Conclusion: The titanium cervical plates can be bent to 6 degree without any crack or weakness of plate. We also assume that minimal bending may increase the resistance to fatigue fracture in cervical flexion-extension movement.

A Nutritional and Pathobiological Inquiry into the Functional Role of Vitamin E -A Study on Levels of Vitamin E in Serum and Liver of the Chick fed Perilla oil diet and Electron Microscopic Examination of the Chick Hepatocyte- (Vitamin E의 기능규명을 위한 영양생화학적 및 병리학적 연구 - 들깨기름 식이를 섭취한 병아리의 헐청과 간의 Vitamin E 수준 및 간세포의 전자현미경 조사를 중심으로 -)

  • Lee, Yang-Cha;Cho, Hea-Young;Kim, Chung-Sook;Han, Seong-Soo
    • Journal of Nutrition and Health
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    • v.15 no.4
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    • pp.277-289
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    • 1982
  • Because of a high degree of unsaturation of perilla seed oil vitamin E deficiency can be produced in chicks by using this particular oil diet. It is therefore convenient to use this oil for elucidating more detailed function (s) of vitamin E. The present study was undertaken to investigate the nutritional and pathological aspects both of high PUFA and of low vitamin E in the diet eventually to elucidate the function(s) of vitamin E. The present study examines the relationships between PUFA, vitamin E and Se in the experimental diets by measuring vitamin E levels both in serum and in liver and activities of SGPT and SGOT and by examining electron micrographs of the chick hepatocytes. Vitamin E concentrations in serum and liver responded to dietary treatments. Serum levels of vitamin E were more affected than those in liver, the values of groups IV(15% fat,- Vit.E) and V(15% fat,-Vit E,-Se) were significantly lower than those of groups I (5% fat) and 111 (15% fat) with P/S ratio of 1. The activities of SGOT and SGPT were found not to be different significantly among different diet groups. Electron microscopic observations of the chick hepatocyte revealed degeneration of mitochondria and appearance of vesicles in the cytoplasm of groups fed diets high in PUFA and deficient in vitamin E alone or deficient in both vitamin E and Se. In the same group pyknotic nuclei and deterioration of the sinusoidal border, showing diminution in microvilli were also observed. More detailed studies concerning biochemical aspects should be carried out by using radioisotopes both in the in vitro and in vivo systems and morphological study should have a focus on the changes in nucleus which seems already suggestive in the present observation.

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