• 한국데이터정보과학회:학술대회논문집
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• 한국데이터정보과학회 2006년도 PROCEEDINGS OF JOINT CONFERENCEOF KDISS AND KDAS
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• pp.213-219
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• 2006

Considering all the factors involved in beef production individual identification using DNA marker testing is the most appropriate solution to give all the breeders' information to the consumers. After taking into account the genealogical information from the Hanwoo, only animals that did not share some parent or grandparent were analysed 33 from the 305 initially sampled. Ten major microsatellite markers were selected from allele amplified and their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

• 농업과학연구
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• 제43권1호
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• pp.33-39
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• 2016

Brassicaceae consists of important species that have significant amounts of metabolites, and many studies have been carried out in order to understand the mechanism that improves the content of these metabolites. In Brassicacea, Cardamine manshurica Nakai is one of the important edible plants and is rich in oil, fiber, and various nutrients. In this study, we constructed cDNA library using leaves from 4 week-old plants and analyzed the ESTs of C. manshurica Nakai. One thousand thirty-nine ESTs were discovered which assembled to form 468 unigenes. The latter contained 116 contigs and 352 singletons. Similarity search of these ESTs with BLASTX revealed similarities with Arabidopsis thaliana 285 (31.9%), Arabidopsis lyrata 172 (19.3%), Capsella rubella 162 (18.1%), and Eutrema salsugineum 137 (15.3%). ESTs were functionally categorized into molecular function, biological process, and cellular component, and each category took 10.6%, 58.5%, and 30.9%, respectively. The functional analysis also found that 94.9% of ESTs showed at least one GO ID. Microsatellite analysis of 468 unigene sequences revealed 225 structures of which Di-, Tri-, Tetra-, Penta-repeats were 35.6% (80/225), 63.1% (142/225), 0.9% (2/225), and 0.4% (1/225), respectively. The results from our study can be a valuable resource for Cardamine research.

• Journal of Species Research
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• 제12권4호
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• pp.281-285
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• 2023

The red-tongue viper(Gloydius ussuriensis) is one of only three species of the genus Gloydius found in South Korea. Gloydius ussuriensis has a narrow activity radius and is distributed nationwide, and this species was reported to have the largest population among the Korean species in genus Gloydius. Preliminary results of a phylogenetic analysis using part of the mitochondrial DNA indicated that domestic G. ussuriensis is not comprised of monophyletic groups, and morphological analysis showed differences between domestic populations. In this study, we developed 17 microsatellites for the analysis of G. ussuriensis genetic diversity based on these characteristics. These microsatellites were developed using six multiplex panels, which could be employed to validate 80 G. ussuriensis specimens from different geographical regions in South Korea. The average number of alleles per locus was 12.2 and ranged from 4 to 25 alleles; the observed heterozygosity ranged from 0.238 to 0.950 and the expected heterozygosity ranged from 0.213 to 0.933. As a result of assessing four inland populations, a high level of genetic diversity was confirmed. These newly developed markers will be useful for further studies on the population structure and evolutionary history of the G. ussuriensis.

• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1234-1240
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• 2009

Gene flow, genetic structure and differentiation of Kutchi, Mehsana and Sirohi breeds of goat from North-Western India were evaluated based on 25 microsatellite markers so as to support breed conservation and improvement decisions. The microsatellite genotyping was carried out using an automated DNA sequencer. The gene diversity across the studied loci for the Kutchi breed varied from 0.57 (ILST 065) to 0.93 (OarFCB 304, OMHC 1, ILSTS 058) with an overall mean of 0.79${\pm}$0.02. The corresponding values for Mehsana and Sirohi breeds were 0.16 (ILST 008) to 0.93 (OMHC 1, ILSTS 058) with an average of 0.76${\pm}$0.04, and 0.50 (ILSTS 029) to 0.94 (ILSTS 058) with an average of 0.78${\pm}$0.02, respectively. The Mehsana breed had lowest gene diversity among the 3 breeds studied. All the populations showed an overall significant heterozygote deficit ($F_{is}$). The Fis values were 0.26, 0.14 and 0.36 for Kutchi, Mehsana and Sirohi goat breeds, respectively. Kutchi and Mehsana were more differentiated (16%) followed by Mehsana and Sirohi (13%).The measures of standard genetic distance between pairs of breeds indicated that the lowest genetic distance was between Kutchi and Sirohi breeds (0.73) and the largest genetic distance was between Mehsana and Kutchi (1.0) followed by Sirohi and Mehsana (0.75) breeds. Mehsana and Kutchi are distinct breeds and this was revealed by the estimated genetic distance between them. All measures of genetic variation revealed substantial genetic variation in each of the populations studied, thereby showing good scope for their further improvement.

• BMB Reports
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• 제38권2호
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• pp.182-190
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• 2005

Genetic heterogeneity of the tropical abalone, Haliotis asinina was examined using randomly amplified polymorphic DNA (RAPD) and microsatellite analyses. One hundred and thirteen polymorphic RAPD fragments were generated. The percentage of polymorphic bands of H. asinina across overall primers was 85.20%. The average genetic distance of natural samples within the Gulf of Thailand (HACAME and HASAME) was 0.0219. Larger distance was observed when those samples were compare with HATRAW from the Andaman Sea (0.2309 and 0.2314). Geographic heterogeneity and $F_{ST}$ analyses revealed population differentiation between H. asinina from the Gulf of Thailand and the Andaman Sea (p < 0.0001). Three microsatellite loci (CUHas1, CUHas4 and CUHas5) indicated relatively high genetic diversity in H. asinina (total number of alleles = 26, 5, 23 and observed heterozygosity = 0.84, 0.42 and 0.33, respectively). Significant population differentiation was also found between samples from different coastal regions (p < 0.0001). Therefore, the gene pool of natural H. asinina in coastal Thai waters can be genetically divided to 2 different populations; the Gulf of Thailand (A) and the Andaman Sea (B).

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.617-621
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• 2006

Recently the numbers of the Thai swamp buffalo (Bubalus bubalis), a native species of Thailand, have been rapidly declining, leading to a requirement for conservation programs for this breed. Such studies of the genetic diversity of this species are essential for conservation decisions and to assist the rational implementation of breeding programs. In this study, the genetic diversity of 80 Thai swamp buffalo, randomly selected from seven different research stations of the Thai Department of Livestock Development, were studied using ten cattle microsatellite markers. Polymorphic PCR products were observed at all microsatellite loci, with percentages of polymorphic loci ranging from 80.00 to 100.00%. The population from Payao showed the lowest level of polymorphism. The mean number of alleles per locus was 4.7 with the highest number of alleles being eight (ETH152) and the lowest being three (HAUT27 and ILSTS030). The average unbiased heterozygosity for all seven populations was 0.61 and varied between 0.5314 (Samui) and 0.6798 (Surin). The genetic distance according to NEI's (1972) ranged from 0.0722 to 0.4427. The populations from Surin and Burirum are the closest populations, while populations from Samui and Payao are the most divergent. The information generated by this study will greatly aid in the establishment of effective breeding and conservation programs for the Thai swamp buffalo.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.85-85
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• 2017

Potato is important carbohydrate source over the world in that revealing high productivity per the unit area, and their cultivation area is estimated to be increased to cope with a scarcity of food according to the population increase. Major cultivated species of potato is Solanum tuberosum (2n = 4x = 48) and regarded as being originated in Andes region of South America. The diverse potato genetic resources has been collected and perserved in Highland Agricultural Research Institute (NICS, RDA), and the genetic materials as DNA stock is conserved in National Agrobiodiversity Center(NAS, RDA). The understanding of genetic constitution of conserved diversity is the basis for the germplam management and further utilization. In this study, we analyzed the genetic diversity of potato germplasm(479 accessions) using 24 microsatellite markers which have been internationally used for fingerprinting of potato accession. The allele number and polymorphic information content (PIC) of total accessions per locus was ranged from 2 to 18 (mean = 8.2) and from 0.214 to 0.771 (mean = 0.595), respectively. Especially, the accession originated from Korea revealed average allele number of 6.0 (2 - 11) and average PIC value of 0.58 (0.193 - 0.763). Three groups were deduced by phylogenic analysis (Group-1, -2, -3); Korean accessions showed close genetic similarity to Japanese and USA accessions, and Korean landraces were mainly included in Group-3. We try to elaborate the genetic diversity analysis of conserved potato germplasm by acquiring more genotypes using applicable molecular markers.

• 생명과학회지
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• 제14권2호
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• pp.339-344
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• 2004

콩에서 경장과 연관된 DNA 표지인자를 개발하여 품종육성에 활용함으로서 육종효율 증진에 기여하고자 수행하였다. 본 시험은 육성된 큰올콩과 신팔달콩의 RIL 계통 및 SSR marker를 이용하여 유전자지도를 작성하고, 이를 바탕으로 경장과 관련된 양적형질 유전자좌(QTt)를 탐색하였다. 시험재료로 이용된 큰올콩과 신팔달콩은 경장이 각각 30.57 cm와 49.75 cm로 매우 큰 차이를 보였다. 경장과 연관된 QTL은 개별마커들과의 분산분석 결과, 연관군 F, J, N 및 O에서 전체변이의 37.83%를 설명할 수 있는 4개의 QTL을 탐색하였다. 특히, 연관군 J와 O에서 각각 14.25%와 10.68%를 설명할 수 있는 주요 QTL을 확인하였다. 따라서 경장 관련 QTL중 연관군 J와 O에서 확인된 주요 QTL은 품종 육성과정에서 경장 관련 선발 마커로서 활용가치가 높은 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.176-182
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• 2019

Objective: This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d'Aquitaine Breeders (AHLBB). Methods: Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results: Mean of effective alleles ($n_e$) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient ($F_{IS}$) value (-0.15). Principal component analysis of estimated genetic distance ($F_{ST}$) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise $F_{ST}$ values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance ($F_{ST}$ values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). $F_{ST(C-E)}$ was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance ($F_{ST}$<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion: This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.

• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.425-429
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• 2010

To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis injections, were evaluated for parentage verification and identity test. These markers were highly polymorphic with a mean of 8.35 alleles per locus and an average expected heterozygosity of 0.711 in 371 individuals. Parentage exclusion probability with only one sampled parent was approximately 0.999. Parentage exclusion probability when another parent' genotype was known was over 0.99999. Overall probability of identity, i.e. the probability that two animals share a common genotype by chance, was $1.52{\times}10^{-16}$. In a test case of parentage assignment, the 17 loci assigned 31 out of 33 cows to the pedigree sires with 95% confidence, while 2 cows were excluded from the paternity relationship with candidate sires. The results demonstrated the high efficacy of the 17 markers in parentage analysis and individual identification for Chinese Holstein cattle.