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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Genetic Heterogeneity of the Tropical Abalone (Haliotis asinina) Revealed by RAPD and Microsatellite Analyses

  • Tang, Sureerat (Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Popongviwat, Aporn (Department of Marine Science, Faculty of Science, Chulalongkorn University) ;
  • Klinbunga, Sirawut (Marine Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)) ;
  • Tassanakajon, Anchalee (Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Jarayabhand, Padermsak (Department of Marine Science, Faculty of Science, Chulalongkorn University) ;
  • Menasveta, Piamsak (Department of Marine Science, Faculty of Science, Chulalongkorn University)
  • Published : 2005.03.31
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Abstract

Genetic heterogeneity of the tropical abalone, Haliotis asinina was examined using randomly amplified polymorphic DNA (RAPD) and microsatellite analyses. One hundred and thirteen polymorphic RAPD fragments were generated. The percentage of polymorphic bands of H. asinina across overall primers was 85.20%. The average genetic distance of natural samples within the Gulf of Thailand (HACAME and HASAME) was 0.0219. Larger distance was observed when those samples were compare with HATRAW from the Andaman Sea (0.2309 and 0.2314). Geographic heterogeneity and $F_{ST}$ analyses revealed population differentiation between H. asinina from the Gulf of Thailand and the Andaman Sea (p < 0.0001). Three microsatellite loci (CUHas1, CUHas4 and CUHas5) indicated relatively high genetic diversity in H. asinina (total number of alleles = 26, 5, 23 and observed heterozygosity = 0.84, 0.42 and 0.33, respectively). Significant population differentiation was also found between samples from different coastal regions (p < 0.0001). Therefore, the gene pool of natural H. asinina in coastal Thai waters can be genetically divided to 2 different populations; the Gulf of Thailand (A) and the Andaman Sea (B).

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