• Asian-Australasian Journal of Animal Sciences
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• v.28 no.2
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• pp.239-246
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• 2015

The present study was conducted to evaluate the effect of the dietary supplementation of Bacillus amyloliquefaciens-based direct-fed microbial (DFM) on growth performance, nutrient utilization, intestinal morphology and cecal microflora in broiler chickens. A total of two hundred and eighty eight 1-d-old Arbor Acres male broilers were randomly allocated to one of four experimental treatments in a completely randomized design. Each treatment was fed to eight replicate cages, with nine birds per cage. Dietary treatments were composed of an antibiotic-free basal diet (control), and the basal diet supplemented with either 15 mg/kg of virginiamycin as antibiotic growth promoter (AGP), 30 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 30) or 60 mg/kg of Bacillus amyloliquefaciens-based DFM (DFM 60). Experimental diets were fed in two phases: starter (d 1 to 21) and finisher (d 22 to 42). Growth performance, nutrient utilization, morphological parameters of the small intestine and cecal microbial populations were measured at the end of the starter (d 21) and finisher (d 42) phases. During the starter phase, DFM and virginiamycin supplementation improved the feed conversion ratio (FCR; p<0.01) compared with the control group. For the finisher phase and the overall experiment (d 1 to 42) broilers fed diets with the DFM had better body weight gain (BWG) and FCR than that of control (p<0.05). Supplementation of virginiamycin and DFM significantly increased the total tract apparent digestibility of crude protein (CP), dry matter (DM) and gross energy during both starter and finisher phases (p<0.05) compared with the control group. On d 21, villus height, crypt depth and villus height to crypt depth ratio of duodenum, jejunum, and ileum were significantly increased for the birds fed with the DFM diets as compared with the control group (p<0.05). The DFM 30, DFM 60, and AGP groups decreased the Escherichia coli population in cecum at d 21 and d 42 compared with control group (p<0.01). In addition, the population of Lactobacillus was increased in DFM 30 and DFM 60 groups as compared with control and AGP groups (p<0.01). It can be concluded that Bacillus amyloliquefaciens-based DFM could be an alternative to the use of AGPs in broilers diets based on plant protein.

• Journal of the Korean Society of Groundwater Environment
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• v.2 no.2
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• pp.85-92
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• 1995

This study was conducted to find out the relationship between microflora in groundwater and its physico-chemical environmental factors at the 59 sites, where distributed at 3 cities and 18 counties in Jeonranam-Do, Korea. The average values of environmental factors were as followings; pH 6.9, temperature 20.1 $^{\circ}C$, dissolved oxygen 6.5 mg/1, the depth of wells 80.1 m, and nitrate 10.7 mg/1. The average value of microbial population size; Heterotrophic bacteria-NA (HPC-NA) and heterotrophic bacteria-YEPD (HPC-YEPD) were 1.4${\times}$10$^3$CFU/ml and 0.59${\times}$10$^3$ CFU/ml respectively HPC-YEPD numbers were 42.1% of HPC-YEPD. Coliform bacteria were detected at 16 sites, and its average numbers were 6.7 CFU/ml. General fungi were detected at 14 sites, and its had average numbers were 3.9 CFU/ml. The correlation coefficient was calculated value as relation index between the microbial population sizes and environmental factors in groundwater. In case of HPC-NA, the correlation coefficient value with dissolved oxygen was 0.087 But it showed negative correlation coefficient with other factors. In case of HPC-YEPD, the correlation coefficient value with pH, temperature and nitrate were 0.0957, 0.0019 and 0.0151 respectively. It was concluded that the population size of HPC-NA were influenced by the order of temperature, dissolved oxygen, pH and the depth of wells and that HPC-YEPD were influenced by the order of pH, dissolved oxygen, nitrate, the depth of wells and temperature.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.453-458
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• 1994

Intestinal microbial flora comprise one third of the large intestinal contents in human. They play a significant effects through beneficial and harmful action on the human health. This is the first study which examined the composition of the microflora of the general population in Korea. Bacteroides, Bifidobacterium, Eubacterium, Peptostreptococcus, Lactobacillus, Streptococcus, Escherichia coli, Staphylococcus, Clostridium perfringens, total aerobic bacteria and total anaerrobic bacteria were counted using various selective and non-selective media. Among the bacteria studied the number of Bifidobacterium were greatest in breast-fed infants(30-90 days old), whereas Streptocuccus and Bifidobacterium in bottle-fed infants. In 20-40 age group Bacteroides were predominant followed by Bifidobacterium and Eubacterium. In early group(over 65 years old) Bacteroides were predominant followed by Eubacterium and bifidobacterium. The frequency and number of Cl. perfringens were highest in dlderly group. These results confirm that the microfloral pattern in large intestine change during the life cycle of humans.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.277-284
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• 2020

This study was undertaken to determine the effects of dietary supplementation with herbal extracts of Portulaca oleracea L. (POL), on the reproductive performance, blood profiles, immune response, and fecal microbial population in multiparous sows. On the basis of initial body weight, a total of 20 multiparous sows (Landrace×Yorkshire; 229.2±1.15 kg) were randomly allotted to 2 treatment groups, each including 10 replicates. The dietary treatments were administered during the lactation period, and included a corn-soybean meal-based diet (control group) and diet supplemented with 0.05% POL. Throughout the experimental period, no significant differences were observed between the two diet groups for body weight, backfat thickness, and reproductive performance. Blood profile analysis revealed significantly decreased lymphocyte concentrations and IL-1β levels (p<0.05), and increased serum IgG levels (p=0.051), of sows consuming the POL supplemented diet. Furthermore, coliform counts tended to decrease in sows consuming diet supplemented with POL (p=0.063). Taken together, our results indicate that POL supplemented diets exert beneficial effects on blood profiles, immune response, and fecal microflora of multiparous sows, without any negative effect on the reproductive performance.

• Applied Biological Chemistry
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• v.40 no.2
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• pp.157-162
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• 1997

A novel compound, named YH-487, was synthesized by attaching the thiol and aminothiazole residue to $C_3$ and $C_7$ position of 7-aminocephalosporanic acid (7-ACA). The therapeutic efficacy on infected animals, pharmacokinetics in vivo and the effect on intestinal microflora of YH-487 were examined. The pharmacokinetics of YH-487 were similar to that of cefotaxime, a third generation ${\beta}-lactam$ antibiotics, in rat. Upon in vivo administration, YH-487 was predominantly delivered to kidney, and mostly excreted through kidney without making any metabolites. The therapeutic efficacy of YH-487 to animal infected with E. coli was three times and twenty times higher than that of cefotaxime and cefotiam, respectively, In vivo administration of YH-487 to Sprague-Dawley rats significantly decreased the population of intestinal gram negative species such as Enterobacteria and Barteroides. However, no significant changes were obseved in gram positive species such as Lactobacillus, Bifidobacteria and Staphylococcus. In addition, continuous administration of YH-487 did not increase the possibility to induce resistant strains in intestinal microflora.

• Journal of Animal Science and Technology
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• v.51 no.6
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• pp.503-510
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• 2009

This study investigated the effects of tannic acid (TA) in the diets for weanling pigs prepared with/without supplemental Fe on performance, hematology, fecal microflora and diarrhea incidence. Limestone and calcium phosphate used in Experiment 1 and 2 were of semi-synthetic and feed-grade quality, respectively; while the trace-mineral premix used in both the experiments was prepared without any added Fe source. In Experiment 1, 108 weaned pigs (Landrace ${\times}$ Yorkshire ${\times}$ Duroc, initially 6.46 ${\pm}$ 1.04 kg BW) were allotted to 3 treatments including control (diet added with $FeSO_4$ and antibiotic), T1 (diet devoid of $FeSO_4$ and antibiotic) and T2 (T1 diet added with 125 mg/kg TA). Each treatment had 4 replicates with 9 pigs in each pen. Feeding of T1 diet had a negative effect on the performance and plasma Fe status of pigs, while addition of TA to T1 diet resulted in performance of pigs comparable to pigs fed the control diet, reduced diarrhea incidence but had a negative influence on the hematological and plasma Fe indices. Additionally, pigs fed T2 diet had fewer (p<0.05) total anaerobic bacteria, Clostridium spp. and coliforms than pigs fed T1 diet, and greater number of Bifidobacterium spp. and Lactobacillus spp. in feces when compared with pigs fed control and T1 diets. In Experiment 2, 144 weaned pigs (Landrace ${\times}$ Yorkshire ${\times}$ Duroc, initially 6.00 ${\pm}$ 1.07 kg BW) were allotted to 4 dietary treatments including control (diet added with $FeSO_4$ and antibiotic) and diets devoid of supplemental Fe added with antibiotic (An), TA and both (AnTA). Each treatment had 4 replicates with 9 pigs in each pen. Addition of An, TA or both to diets devoid of supplemental Fe did not have any effect on performance, blood hematology and plasma Fe but resulted in reduced (p<0.05) diarrhea incidence and lower (p<0.05) fecal coliform population than pigs fed the control diet. These results suggest that TA has a negative influence on blood hematology and plasma Fe status when diets are inadequate in Fe; however, TA reduced diarrhea incidence and might have antimicrobial activity.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.1
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• pp.94-98
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• 1997

This experiment was conducted to investigate the distribution of the soil microflora of Daekwanryung highlands of Kwangwon Province. It was found that soil microorganisms each as Bacteria, Actinomycetes, Fungi, Pseudomonas spp. and Erwnia sp. were mostly in the top 0-15 cm profile. It also was found that soil microflora population was affected in many ways by kind of cropping plant. The number of Erwinia sp. that is one of the soil born plant pathogenic bacteria was more abundant in potato field than chinese cabbage soil. It was also studied difference of mountain and grassland soils from cropping soils.

• Korean Journal of Poultry Science
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• v.33 no.3
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• pp.203-209
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• 2006

This study were conducted to evaluate effects of drinking concentrated liquid chitosan on performance of broiler. A total of 240 broiler chicks at 7 days of age were fed the commercial diet and water until 47 days of age, drinking water divided into three treatments, 0 ppm (control), 200 ppm (200) and 400 ppm (400) of concentrated liquid chitosan. The body weight gain and feed conversion ratio were not different by drinking concentrated liquid chitosan until 28 days of age, but body weight gain and feed conversion ratio were improve by drinking concentrated liquid chitosan over 28 days of age. Both body weight gain and feed conversion ratio were tend to improve and significantly improved (p<0.05) in 200 and 400 than control at 29 to 35 days of age, respectively. While body weight gain was tended to improve and significantly improved (p<0.05) in 400 and 200 than control at 36 to 47 days of age, respectively. There was a decrease in the microflora population of E coli. in the cecum contents and feaces of broilers by drinking concentrated liquid chitosan. This effect is higher in 400 than 200. The digestibility of dry matter and crude fat of feed were tend to increased in broiler by drinking concentrated liquid chitosan. Digestibility of crude protein of feed of broiler in 200 and 400 was significantly improved (p<0.05) as compared with those in control. These results indicated that when broiler chicks drinking concentrated liquid chitosan of 400 ppm, the improvement of the body weight gain, feed conversion ratio and feed nutrients digestibility, decreasing of microflora population of both E coli. and Salmonella in the cecum contents and feaces.

• Korean Journal of Poultry Science
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• v.31 no.1
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• pp.25-31
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• 2004

The effect of drinking rhus tree-extract on performance of broiler was performed to investigate development of natural antibiotic in process of broilers production. A total of 320 broiler chicks at 1 day of age were fed the commercial diet and water, drinking water containing 0 ppm(control), 500 ppm (T1), 1,000 ppm (T2) and 2,000 ppm (T3) of rhus tree-extract fur seven weeks. The body weight gain and fred conversion ratio were not different by drinking rhus tree-extract until 35 days of age, but body weight gain and feed conversion ratio were tend to improve by drinking rhus tree-extract over 35 days of age, the improvement was higher in both T$_1$, and T$_3$ than control group(p＜0.05) when 35 to 42 days of age. There was a decrease in the microflora population of both E. coli. end Salmonella in the cecum contents and feaces in broilers by drinking rhus tree-extract. The digestibility of dry matter, crude fat, and crude ash of feed were tend to increased in broiler drinking rhus tree-extract. Digestibility of crude protein of feed in broiler three treated groups was significantly improved (p＜0.15) as compared with those in control group. These results indicated that the drinking rhus tree-extract were effective in the body weight gain, feed conversion ratio, decreasing of microflora population of both E. coli and Salmonella in the cecum contents and feaces, and it had also effective the digestibility of nutrients in broilers.

• Journal of Korean Society of Forest Science
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• v.90 no.6
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• pp.767-773
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• 2001

We investigated the impacts of the fairy-ring of Tricholoma matsutake on the dynamics of soil microflora and soil enzyme activities by grouping the soils around the fairy-ring of T. matsutake into four regions. The regions were grouped as 'zone of decayed mycorrhizae', 'zone of mycorrhizae for fruiting', 'zone of physiologically active mycorrhizae' and 'zone free from mycorrhizal infection'. Soil fungi and actinomycetes were quite little at the soils around the fairy-ring of T. matsutake compared to those of general forest soils, and there were significant differences among the four regions. The soils with the mycelial cluster of T. matsutake showed about one third of microbial population compared to those in the zone free from mycorrhizal infection, which indicated that T. matsutake took a dominant position within the fairy-ring of the fungus. We could manifest that T. matsutake showed a distinctive characteristics of mycorrhizal fungus since the activities of dehydrogenase were significantly different between the zone of physiologically active mycorrhizae and the zone free from mycorrhizal infection. The dehydrogenase activity was the highest at the early season of fruiting around the fairy-ring of T. matsutake, while the acid-phosphatase activity increased from March to June followed by a slight decrease on August and peaked on October. This phenomenon made us infer that the vitality of T. matsutake be sustained after fruiting.