• Korean Journal of Agricultural Science
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• v.48 no.1
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• pp.179-189
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• 2021

Plants need essential mineral elements to favorably develop and to complete their life cycle. Despite the irreplaceable roles of microelements, they are often ignored due to the relative importance of macroelements with their influence on crop growth and development. We focused on the changes in primary metabolites in the leaves and roots of bell pepper plants under 6 microelements-deficient conditions: Copper (Cu), Zinc (Zn), Iron (Fe), Manganese (Mn), Boron (B) and Molybdenum (Mo). Bell pepper plants were grown in hydroponic containers, and individual elements were adjusted to 1/10-strength of Hoagland nutrient solution. A remarkable perturbation in the abundance of the primary metabolites was observed for the Fe and B and the Mn and B deficiencies in the leaves and roots, respectively. The metabolites with up-accumulation in the Fe-deficient leaves were glucose, fructose, xylose, glutamine, asparagine and serine. In contrast, the Mn deficiency also resulted in a higher accumulation of glucose, fructose, xylose, galactose, serine, glycine, β-alanine, alanine and valine in the roots. The B deficiency noticeably accumulated alanine, valine and phenylalanine in the roots while it showed a substantial decrease in glucose, fructose and xylose. These results show that the primary metabolism could be seriously disturbed due to a microelement deficiency, and the alteration may be either the specific or adaptive responses of bell pepper plants.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.494-499
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• 2011

Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing $130g{\cdot}L^{-1}$ sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing $130g{\cdot}L^{-1}$ sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at $4^{\circ}C$ without media. Among the various medium concentrations used ($0.25{\times}$, $0.5{\times}$, $1.0{\times}$, $2.0{\times}$, and $4.0{\times}$ NLN liquid medium), $0.5{\times}$ NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$, $0.5{\times}$, and $1.0{\times}$ NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$ NLN microelements, followed by incubation at $25^{\circ}C$ for 30 days.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.237-242
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• 2016

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.43-49
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• 1995

The main purpose of these studies were to clarify differences in Cheju - scoria and other solid media on quantity and quality of cherry tomatoes and on shift of component of the nutrient solution, and to use Practically Cheju-scoria as an excellent solid culture medium. The results obtained were summarized as follows ; 1. Among scoria plots, the rates of dry weights, fruits and their sugar-acid ratio were higher in the plot that was drained well with deep flow for one hour once a day. 2. Fresh fruit weights were lighter in rockwool and deep flow technique, but larger in scoria, Hyugashi (artificial gravel, $\Phi$10-12mm) and perlite in moving to higher flower cluster. 3. The results of analysis on microelement among solution components showed decrease of concentrations of P and K in the period of growth and development. 4. Yields and brix of cherry tomato showed a tendency to increase in rockwool and Hyugashi than anothers. 5. The concentration of fertilizer base was increased in general solution culture. Transpiration and absorption were similar in scoria plot and other media. 6. More studies of the Cheju-scoria development is required in order to use it as a solid medium for solution culture.

• Journal of Forest and Environmental Science
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• v.29 no.4
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• pp.300-306
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• 2013

Based on the guiding ideology of "Close-to-nature forestry", the soil chemical properties of Korean pine (Pinus koraiensis) plantation forest which was early done by canopy gap control were analyzed of Liangshui nature reserve in northeastern China. The results indicated that the nurture of forestry crevice diaphanous tended to improve the soil nutrient contents and significant differences of soil nutrients existed among different levels of soil for the same forest type of Korean pine. At 0< $H{\leq}20$ cm layer, the content of available nitrogen, available phosphorus, available potassium, total nitrogen and total phosphorus in artificial pure Korean pine forest are 640.28 $mg{\cdot}kg^{-1}$, 7.54 $mg{\cdot}kg^{-1}$, 275.91 $mg{\cdot}kg^{-1}$, 1.114% and 0.075%, they all higher than the other kinds of forests and for average 1.1 times, 1.4 times, 1.3 times, 1.6 times and 1.2 times. From the layer of $0<H{\leq}20$ cm to 20 cm < $H{\leq}40$ cm, soil nutrient indicators showed various degrees of decreasing in which organic matter had the greatest decline, decreasing by 170.64% while PH had the lowest decline, decreasing by 4.66%.

• Korean Journal of Microbiology
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• v.5 no.2
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• pp.61-68
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• 1967

In the process of the incorporation of orthophsphate into protein and other cell constituents, the role of inorganic polyphosphate and RNA-polyphosphate complex and the correlation between them were pursued by analyzing the contents of $^{32}P$ and total P in various fractions of Chlorella cells, which had been uniformly labeled with $^{32}P$ before the inoculation in a normal "cold" medium or P-free medium during the culture. The effects of ionizing radiation and various micronutritional-element deficiencies on the phosphate incorporation into, and biosynthesis of, protein and other introgenus compounds in the cells were also observed. When the uniformly $^{32}P$-labeled algae were grown in a normal "cold" medium the contents of $^{32}$ P in the fractions of protein, DNA and RNA-polyphosphate complex increased, but those in the fraction of acid-insoluble polyphosphate decreased. On the other hand, amount of $^{32}P$in the fraction of RNA was almost unchanged in spite of rapid increase of the total P. In the growing period of $^{32}P$-labeled algae in a P-free medium, amounts of $^{32}P$ in the fractions of DNA, protein and lipid increased, while those in the fractions of RNA-polyphosphate and inorganic polyphosphates decreased. When the algal cells were irradiated with about 70, 000r of gamma-rays before the inoculation in the medium, amounts of phosphate in the fractions of DNA, RNA, nucleotides and protein decreased during the culture, compared with those of the control. However, the phosphate content in the fraction of acid-insoluble polyphosphate of the irradiated cells increased than those of the control. In the growing period of the algae in a Mo-free, medium, amounts of acid-soluble total phosphate and nucleotides of the cells increased, while the amounts of residual protein and RNA decresed compared with those of the normal cells. Amounts of alkali-labile protein and phospholipid of the cells grown in a B-free medium decreased, whereas amount of phosphate in acid-soluble fraction increased compared with the control. In general, the contents of protein and RNA in each microelement deficient cells decreased more or less, compared with those in the normal cells.in the normal cells.

• Journal of Korean Society of Disaster and Security
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• v.12 no.4
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• pp.43-52
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• 2019

To analyze the influence on the stability, resulting from application of upgrade pipe roof structure (UPRS) method to the structure existed under subway Station, physical properties of a ground, elasticity and elasto-plastic theories, including displacement analysis of finite elements, stress analysis of finite elements, displacement caused by steel pipe propulsion and internal excavation, and stress change in a steel pipe, were introduced. Then, the influence on structural stability when applying the UPRS method was compared and reviewed based on the construction management standard of the Ministry Land, Infrastructure and Transport and foreign sources, using numerical analysis with a model which assumes that each microelement divided into a structurally stable point consists of the connection of finite points. As a result of the finite element analysis, 7.21 mm maximum displacement, 1/3,950 angular displacement, 70.28 MPa bending compressive stress of steel pipe structure constructed with UPRS (non-excavation) method and 477.38 MPa maximum shear strength were within their allowable standards (25.00 mm, 1/500, 210.00 MPa and 120.00 MPa, respectively), and therefore, the results showed that the design and construction are stable.

• Journal of Bio-Environment Control
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• v.11 no.4
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• pp.168-174
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• 2002

A new circulating hydroponic system was invented for oriental melons grown in the greenhouse. For developing nutrient solution management techniques, we examined the changes of nutrient contents of circulating solution in three different types of new hydroponic systems. The yield and fruit quality of oriental melons in Hydroponics were better than those in soil culture. The substrate culture was appropriate fer hydroponics of oriental melons, and NFT was turned to be the opposite due to the physiological disorder during hot seasons. Yamazaki's melon solution with EC 2.0dS.m$^{[-10]}$ was the most appropriate for oriental melons. The new circulating hydroponic system seemed to be appropriate for oriental melons because of the stable EC, pH and the macro- and micro-element contents. NO$_3$-N, Ca and Mg contents in the circulating solution kept a good balance in all types of hydroponics. However, p content, compared to other types, decreased by the degree of 1 me.L$^{[-10]}$ in perlite medium. K content showed irregular status in perlite but showed the stable status in cocopeat. Generally, microelements, except Mo, showed stable absorption in the substrate culture. However, in NET, most of the elements showed irregular absorption except B and Mn. Microelement absorption, especially Cu, Zn and Mo, decreased during hot seasons.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.123-131
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• 1999

To get the basic information for the improvement of the optimum levels of upland soil fertility and fertilizer application, the soil samples in two hundred fifteen site were collected from the major condiment vegetable cultivation areas such as red pepper, garlic and onion fields. Physico-chemical properties of the soil samples were investigated. Soil texture distribution of soil samples in red pepper, garlic and onion cultivation areas was in order of loam (L), sandy loam (SL), silt loam (SiL) and clay loam (CL) (35.4, 31.6, 14.9 and 7.0%, respectively). The average pH of soil in garlic and onion cultivation areas were over pH 6.0, whereas in red pepper was under pH 5.5. The frequency distribution of soil pH in total sampling sites were 58.7% in under pH 6.0 and 21.4% in below pH 5.0, in contrast to 10.3% in over pH 7.0. The organic matter contents were in the range of $20{\sim}30g\;kg^{-1}$, and the onion field soils was a little higher than those in red pepper or garlic. The available phosphate contents were in the range of $719{\sim}746mg\;kg^{-1}$ and were not different among in red pepper, garlic and onion. The frequency distribution of available phosphate in total sampling sites were found 62.8% of above $600mg\;kg^{-1}$, which was over the standard level for upland soil improvement, and then 22.3% was exceeded $1,000mg\;kg^{-1}$, especially. In the exchangeable cations, the K and Ca contents in garlic (1.27 and $9.11cmol\;kg^{-1}$) and onion (1.20 and $8.39cmol\;kg^{-1}$) were higher than in red pepper (0.96 and $5.87cmol\;kg^{-1}$), respectively. The Mg contents in garlic field soils ($2.17cmol\;kg^{-1}$) were higher than those in red pepper and onion (1.51 and $1.80cmol\;kg^{-1}$). This exchangeable K, Ca and Mg contents were higher than the standard level for upland soil improvement. The contents of microelement were ranged in $54.3{\sim}60.1mg\;kg^{-1}$ for Fe, $31.3{\sim}42.3mg\;kg^{-1}$ for Mn, $1.7{\sim}2.3mg\;kg^{-1}$ for Cu and $4.8{\sim}5.5mg\;kg^{-1}$ for Zn, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.631-636
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• 2010

One experiment was carried out to investigate the soil chemical properties of bracken growth and the inorganic element contents of plant. To the results of soil analysis in native bracken (Pteridium aquilinum L.) growth, soil pH was 5.2, organic matter was 19 g $kg^{-1}$ and available phosphate was 20 mg $kg^{-1}$, and exchangeable potassium, calcium, magnesium were 0.32, 2.0 and 1.3 $cmol_c\;kg^{-1}$, respectively. In the bracken cultivation soil, pH was 5.7, organic matter was 13 g $kg^{-1}$ and available phosphate 367 mg $kg^{-1}$, and exchangeable potassium, calcium and magnesium were 0.81, 4.0 and 1.4 $cmol_c\;kg^{-1}$, respectively. The soil pH, available phosphate and exchangeable calcium were much lower in bracken native soil than those of cultivation soil, while organic matter was a little higher in native soil than that of cultivation soil. In native bracken plants, three major elements of nitrogen, phosphorus and potassium, were 4.40, 0.55 and 3.40%, calcium and magnesium were 0.22 and 0.32%, and microelements of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron were 126, 210, 23, 75 and 11 mg $kg^{-1}$, respectively. In cultivation bracken, three major elements of nitrogen, phosphorus and potassium, were 5.50, 0.73 and 3.55%, calcium and magnesium were 0.17 and 0.28%, and microelement contents of iron (Fe), manganese (Mn), copper (Cu), zinc (Zn) and boron (B) were 120, 252, 19, 72 and 20 mg $kg^{-1}$, respectively.