Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Microspore-derived Embryo Formation in Response to Cold Pretreatment, Washing Medium, and Medium Composition of Radish (Raphanus sativus L.)

  • Chun, Chang-Hoo (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Na, Hae-Young (Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Received : 2011.09.28
  • Accepted : 2011.10.04
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun) flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing $130g{\cdot}L^{-1}$ sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing $130g{\cdot}L^{-1}$ sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment, microspore viability was maintained for 24 h at $4^{\circ}C$ without media. Among the various medium concentrations used ($0.25{\times}$, $0.5{\times}$, $1.0{\times}$, $2.0{\times}$, and $4.0{\times}$ NLN liquid medium), $0.5{\times}$ NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$, $0.5{\times}$, and $1.0{\times}$ NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on $0.5{\times}$ NLN liquid medium supplemented with $0.25{\times}$ NLN microelements, followed by incubation at $25^{\circ}C$ for 30 days.

Keywords

References

  1. Burnett, L., S. Yarrow, and B. Huang. 1992. Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. oleifera. Plant Cell Rpt. 11:215-218.
  2. Chen, W.H., C.Y. Tang, and Y.L. Kao. 2009. Ploidy doubling by in vitro culture of excised protocorms or protocorm-like bodies in Phalaenopsis species. Plant Cell Tissue Organ Cult. 98:229-238. https://doi.org/10.1007/s11240-009-9557-3
  3. Clement, C., R. Sangwan, and B. Sangwan-Norreel. 2005. Microspore embryo induction and development in higher plants: Cytological and ultrastructural aspects. Biotechnol. Agr. For. 56:53-72.
  4. Curtis, I.S. 2009. Radish, p. 381-389. In: E.C. Pua and M.R. Davey (eds.). Biotechnology in agriculture and forestry. Springer, Dordrecht.
  5. Curtis, I.S. 2011. Genetic engineering of radish: Current achievements and future goals. Plant Cell Rpt. 30:733-744. https://doi.org/10.1007/s00299-010-0978-6
  6. Gamborg, O.L., R.A. Miller, and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Expt. Cell Res. 50:151-158. https://doi.org/10.1016/0014-4827(68)90403-5
  7. Jardinaud, M.F., A. Souvre, M. Beckert, and G. Alibert. 1995. Optimization of DNA transfer and transient $\beta$-glucuronidase expression in electroporated maize (Zea mays L.) microspores. Plant Cell Rpt. 15:55-58. https://doi.org/10.1007/BF01690253
  8. Keller, W.A. 1984. Anther culture of Brassica, p. 302-310. In: I.K. Vasil (ed.). Cell culture and somatic cell genetics of plants. Academic Press, New York.
  9. Lichter, R. 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z. Pflanzenphysiol. 105:427-434. https://doi.org/10.1016/S0044-328X(82)80040-8
  10. Loeb, T.A. and T.L. Reynolds. 1994. Transient expression of the uidA gene in pollen embryoids of wheat following microprojectile bombardment. Plant Sci. 104:81-91. https://doi.org/10.1016/0168-9452(94)90193-7
  11. Mishiba, K., T. Ando, M. Mii, H. Watanabe, H. Kokubun, G. Hashimoto, and E. Marchesi. 2000. Nuclear DNA content as an index character discriminating taxa in the genus Petunia sensu Jussieu (Solanaceae). Ann. Bot. 85:665-673. https://doi.org/10.1006/anbo.2000.1122
  12. Na, H. and C. Chun. 2009. Nutritional, chemical and physical factor affecting somatic embryo formation and germination in Pimpinella brachycarpa. Kor. J. Hort. Sci. Technol. 27: 280-286.
  13. Olmedilla, A. 2010. Microspore embryogenesis, p. 27-44. In: E.C. Pua and M.R. Davey (eds.). Plant developmental biologybiotechnological perspectives, Springer, Dordrechf.
  14. Prem, D., K. Gupta, and A. Agnihotri. 2005. Doubled haploids:a powerful biotechnological tool for genetic enhancement in oilseed Brassicas, p. 18-30. In: P.S. Srivastava, A. Narula, and S. Srivastava (eds.). Plant biotechnology and molecular markers. Anamaya Publishers, New Delhi.
  15. Sangwan, R.S., C. Ducrocq, and B. Sangwan-Norreel. 1993. Agrobacterium-mediated transformation of pollen embryos in Datura innoxia and Nicotiana tabacum: Production of transgenic haploid and fertile homozygous dihaploid plants. Plant Sci. 95:99-115. https://doi.org/10.1016/0168-9452(93)90083-C
  16. Sato, S., N. Katoh, S. Iwai, and M. Hagimori. 2002. Effect of low temperature pretreatment of buds or inflorescence on isolated microspore culture in Brassica rapa (syn. B. campestris). Breed. Sci. 52:23-26. https://doi.org/10.1270/jsbbs.52.23
  17. Takahashi, Y., S. Yokoi, and Y. Takahata. 2011. Improvement of microspore culture method for multiple samples in Brassica. Breed. Sci. 61:96-98. https://doi.org/10.1270/jsbbs.61.96
  18. Wedzony, M., B.P. Forster, I. Zur, E. Golemiec, M. Szechynska- Hebda, E. Dubas, and G. Gotebiowska. 2009. Progress in doubled haploid technology in higher plants, p. 1-33. In: A. Touraev, B.P. Forster, and S.M. Jain (eds.). Advances in haploid production in higher plants. Springer, Dordrechf.
  19. Yao, Q.A., E. Simion, M. William, J. Krochko, and K.J. Kasha. 1997. Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.). Genome 40:570-581. https://doi.org/10.1139/g97-075