• 제목/요약/키워드: Microbial Biotechnology

검색결과 2,201건 처리시간 0.027초

Genome Snapshot of Paenibacillus polymyxa ATCC $842^T$

  • Jeong, Hae-Young;Kim, Ji-Hyun;Park, Yon-Kyoung;Kim, Seong-Bin;Kim, Chang-Hoon;Park, Seung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제16권10호
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    • pp.1650-1655
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    • 2006
  • Bacteria belonging to the genus Paenibacillus are facultatively anaerobic endospore formers and are attracting growing ecological and agricultural interest, yet their genome information is very limited. The present study surveyed the genomic features of P. polymyxa ATCC $842^T$ using pulse-field gel electrophoresis of restriction fragments and sample genome sequencing of 1,747 reads (approximately 17.5% coverage of the genome). Putative functions were assigned to more than 60% of the sequences. Functional classification of the sequences showed a similar pattern to that of B. subtilis. Sequence analysis suggests nitrogen fixation and antibiotic production by P. polymyxa ATCC $842^T$, which may explain its plant growth-promoting effects.

Current Status of Microbial Phenylethanoid Biosynthesis

  • Kim, Song-Yi;Song, Min Kyung;Jeon, Ju Hyun;Ahn, Joong-Hoon
    • Journal of Microbiology and Biotechnology
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    • 제28권8호
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    • pp.1225-1232
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    • 2018
  • Phenylethanoids, including 2-phenylethanol, tyrosol, and salidroside are a group of phenolic compounds with a C6-C2 carbon skeleton synthesized by plants. Phenylethanoids display a variety of biological activities, including antibacterial, anticancer, anti-inflammatory, neuroprotective, and anti-asthmatic activities. Recently, successful microbial synthesis of phenylethanoids through metabolic engineering and synthetic biology approaches has been reported and could allow phenylethanoid production from alternative microbial sources. Here, we review the recent achievements in the synthesis of phenylethanoids by microorganisms. The work done so far will contribute to the production of diverse phenylethanoids using various microbial systems and facilitate exploration of further diverse biological activities of phenylethanoids.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

  • 이정숙;정민철;김우식;이근철;김홍중;박찬선;이헌주;주윤정;이근종;안종석;박완;박용하;민태익
    • 한국미생물·생명공학회지
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    • 제24권2호
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    • pp.234-241
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    • 1996
  • Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

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Symbiobacterium toebii Sp. nov., Commensal Thermophile Isolated from Korean Compost

  • Sung, Moon-Hee;Bae, Jin-Woo;Kim, Joong-Jae;Kim, Kwang;Song, Jae-Jun;Rhee, Sung-Keun;Jeon, Che-Ok;Choi, Yoon-Ho;Hong, Seung-Pyo;Lee, Seung-Goo;Ha, Jae-Suk;Kang, Gwan-Tae
    • Journal of Microbiology and Biotechnology
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    • 제13권6호
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    • pp.1013-1017
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    • 2003
  • A thermophilic nonspore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The microorganism, designated as $SC-1^T$, was identified as a nitrate-reducing and nonmotile bacterium. Although the strain was negatively Gram-stained, a KOH test showed that the strain $SC-1^T$ belonged to a Gram-positive species. Growth was observed between 45 and $70^{\circ}C$. The optimal growth temperature and pH were $60^{\circ}C$ and pH 7.5, respectively. The G+C content of the genomic DNA was 65 mol% and the major quinone types were MK-6 and MK-7. A phylogenetic analysis based on 16S rDNA sequences revealed that the strain $SC-1^T$ was most closely related to Symbiobacterium thermophilum. However, the level of DNA-DNA relatedness between strain $SC-1^T$ and the type strain for Symbiobacterium thermophilum was approximately 30%. Accordingly, on the basis of the phenotypic traits and molecular systematic data, the strain $SC-1^T$ would appear to represent a new species within the genus Symbiobacterium. The type strain for the new species is named $SC-1^T$ ($=KCTC\;0307BP^T;\;DSM15906^T$).

Potential Application of the Recombinant Escherichia coli-Synthesized Heme as a Bioavailable Iron Source

  • Kwon, Oh-Hee;Kim, Su-Sie;Hahm, Dae-Hyun;Lee, Sang-Yup;Kim, Pil
    • Journal of Microbiology and Biotechnology
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    • 제19권6호
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    • pp.604-609
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    • 2009
  • To investigate the potential use of microbial heme as an iron source, recombinant Escherichia coli coexpressing ALA synthase (HemA) as well as the NADP-dependent malic enzyme (MaeB) and dicarboxylic acid transporter (DctA) were cultured. The typical red pigment extracted from the recombinant E. coli after 38 h showed highest absorbance at 407 nm, and the amount of iron in 38.4 mg of microbial heme extract derived from 6-1 fermentation broth was 4.1 mg. To determine the commercial potential of the recombinant E.coli-synthesized iron-associated heme as an iron source, mice were fed the iron-free provender with the microbial heme extract. The average body weight reduction of mice fed non-iron provender was 2.3%, whereas no detectable weight loss was evident in mice fed microbial heme addition after 15 days. The heme content of the blood from microbial heme fed mice was 4.2 mg/ml whereas that of controls was 2.4 mg/ml, which implies that the microbial heme could be available for use as an animal iron source.