• Transactions of the Korean Society of Mechanical Engineers B
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• v.34 no.5
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• pp.523-529
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• 2010

Recently, several research groups have been investigating the motion of flagellated bacteria, with the aim of examining the feasibility of using bacterial chemotaxis as an efficient power source for microactuators. In this study, microparticle-tracking velocimetry ($\mu$-PTV) is used for investigating the motion of fluorescent microbeads propelled by bacterial chemotaxis. Flagellated bacteria, Serratia marcescens, are spontaneously attached to the surface of the fluorescent polystyrene (PS) microbeads in an aqueous culture. The microbeads thus treated are injected into the test medium, which contains the solidified chemoattractant L-aspartate. With time, the particles slowly move toward the zone in which the L-aspartate concentration is high. This study shows that chemotaxis of flagellated bacteria can be applied as an efficient power source for microactuators.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.23 no.6
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• pp.555-560
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• 2014

This paper presents a microparticle separator using a spiral microchannel. A particle separator based on the dean vortex was designed, fabricated, and characterized. Two different spiral microchannels were fabricated. Width and initial radius of rotation in the spiral microchannel were fixed to $300{\mu}m$ and 1.75 mm, respectively. Two different depths of the microchannels were designed at $50{\mu}m$ and $80{\mu}m$. In this experimental study, the equilibrium position of microparticles was monitored by using fluorescent microbeads. In the case of a low dean number (<1.0), lift force and dean drag force were similar, indicating that microbeads were distributed to almost all areas across microchannels. However, in the case of a high dean number (>1.0), dean drag force rather than lift force was dominant, indicating that microbeads moved toward the inner wall of the spiral microchannel.

• Applied Chemistry for Engineering
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• v.18 no.6
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• pp.599-602
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• 2007

23 F/cc grade new type of high density activated carbon from crystalline green cokes type of mesocarbon microbeads has been synthesized by chemical and electrochemical activation. In order for these materials to have high performance, electrochemical behavior during electrochemical activation has been investigated by sequential voltage applying schemes. These results showed that the effective voltage for electrochemical activation was about 2.7~3.2 V irrelevant to applying voltage due to the decrease of surface activation of activated carbon with high specific surface area.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.100-100
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• 2003

• 한국전기화학회:학술대회논문집
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• 2000.04a
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• pp.3-4
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• 2000

• Bulletin of Food Technology
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• v.22 no.3
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• pp.577-586
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• 2009

• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.229-233
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• 1995

Acremonium chrysogenum was immobilized in ionotropic gel beads to develop semi-continuous production of cephalosporin C (CPC). Barium alginate beads were more stable than calcium alginate or strontium alginate beads in chemically defined media. The gel stability of Ba-alginate was further increased by cross-linking with polyethyleneimine (PEI). The presence of carboxymethyl cellulose inside Ba-alginate beads did not reduce mass transfer resistance. Ba-alginate microbeads that had little diffusion limitation increased CPC production rate 1.6 fold higher than that of normal beads. CPC fermentation with immobilized cells in Ba-alginate microbeads was performed continuously for 40 days by way of repeated fed-batch operations. Mathematical modeling was developed to describe the repeated fed-batch fermentation system. Results of the computer simulation agreed well with the experimental data, which made it possible to predict an optimal feeding rate that could maximize total CPC productions.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.48 no.4
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• pp.102-107
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• 2011

In this paper, a microfluidic array biochip for simultaneously detecting multiple antigen-antibody bindings was designed and implemented. The biochip has the single channel in which microreaction chambers are serially connected, and the antibody-coated microbeads are packed in each microreaction chamber. In addition, the weir structure was fabricated in the microchannel using the gray-scale photolithography in order to trap the microbeads in the microreaction chamber. Three kinds of antibodies were chosen, and the antibodies were immobilized onto the microbeads by the streptavidin-biotin conjugation. In the experiment, as the fluorescence-labeled antigens were injected into the microchannel, the antigen-antibody bindings were completed in 10 minutes. When the solution with multiple antigens was injected into the microchannel, it was observed that the fluorescence intensity increased in only the corresponding microreaction chambers with few non-specific binding. The microfluidic array biochip implemented in this study provides, even with the consumption of tiny amount of sample and fast reaction time to simultaneously detect multiple immunoreactions.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.254.2-254.2
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• 2007

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.16.2-16.2
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• 2008