• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1323-1329
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• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.215-223
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• 2019

Recently, it has become known that Gotjawal terrain is an important area, therefore the study on this area has conducted in the geological, ecological, and cultural aspects. The studies on bryophytes of Gotjawal, however, have not been sufficiently performed. This study presents a survey on the bryophytes of the evergreen broad-leaved forest of Cheongsu-ri at Hangyeong Gotjawal in the southwestern part of Jejudo Island, Korea. A total of 72 taxa belonging to the Bryophyta (18 families 43 genera 55 species) and Marchantiophyta (11 families 13 genera 17 species) were determined and the liverwort index was 23.6%. Predominant life-form was weft. The rates of the bryophytes dominating in mesic to hygric sites were higher than the bryophytes mainly observed in xeric habitats. These values indicate that the forests are widespread and dense in this study area. Upon an investigation of the substrates, the bryophytes on rocks were most diverse. The results appear to stem from the fact that volcanic rock masses of various sizes lay scattered over the study area, offering numerous micro-habitats for bryophyte due to one of the characteristics of Gotjawal. We suggest that more detailed studies should be conducted at the regional scale to establish the bryophyte flora of Gotjawal and the evergreen broad-leaved forests on Jejudo Island.

• Advances in nano research
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• v.7 no.2
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• pp.109-123
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• 2019

In this article, the free vibration analysis of annular sandwich plates with various functionally graded (FG) porous cores and carbon nanotubes reinforced composite (CNTRC) facesheets is investigated based on modified couple stress theory (MCST) and first order shear deformation theories (FSDT). The annular sandwich plate is composed of two face layers and a functionally graded porous core layer which contains different porosity distributions. Various approaches such as extended mixture rule (EMR), Eshelby-Mori-Tanaka (E-M-T), and Halpin-Tsai (H-T) are used to determine the effective material properties of microcomposite circular sandwich plate. The governing equations of motion are extracted by using Hamilton's principle and FSDT. A Ritz method has been utilized to calculate the natural frequency of an annular sandwich plate. The effects of material length scale parameters, boundary conditions, aspect and inner-outer radius ratios, FG porous distributions, pore compressibility and volume fractions of CNTs are considered. The results are obtained by Ritz solutions that can be served as benchmark data to validate their numerical and analytical methods in the future work and also in solid-state physics, materials science, and micro-electro-mechanical devices.

• Journal of Acupuncture Research
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• v.38 no.4
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• pp.325-330
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• 2021

In this Case Report, a patient with Buerger's disease who had a leg amputation below his lower right knee and a vascular bypass of right leg, developed a wound caused by his prosthetic leg and subjective discomfort. The patient received skin flap surgery but the wound did not heal properly. He was admitted to the Korean Medicine Hospital where his wound, right leg coldness, and phantom pain were treated with combined Korean medicine. The patient was hospitalized again where he underwent micro-drilling surgery. The patient was re-admitted to the Korean Medicine Hospital where he received combined Korean medicine treatment (CKMT) and carbon arc light treatment (CALT) for his wound, leg coldness, stiffness, and hypoplasia. The temperature of his right leg increased, the numeric rating scale score for assessing pain fell from 5 to 1.5, and subjective discomfort was reduced (< 20%) suggesting this may be an effective treatment.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.12
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• pp.115-125
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• 2019

Most of construction at urban area is now undertaking a redevelopment because currently the most of city is full. But the research about the collision of urban tissue at urban redevelopment area are very limited and there are many issues at urban redevelopment area. So the purpose of this study is to analyze the collision between different urban tissue at urban redevelopment area and suggest proper method for urban redevelopment. The area inside of four main gates, especially Chung Jin, Gong pyeong, Mu gyo- Da dong district are selected to analyze because the area inside of these gates is the oldest urban redevelopment area and each of them has a different redeveloped method. And current status of research area's urban tissue and collision situation are analyzed by macro and micro scale of view. The results of this study were as follows; Ignoring the timeline of the urban redevelopment process can be a major issue in collision between urban tissue. Therefore, the consideration of intermediate stage of redevelopment process is needed by making public area, a temporal use of vacant lot and changing architectural elements can make a harmony between different urban tissue.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.10
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• pp.129-135
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• 2022

This study aims to identify the factors supporting and inhibiting the digitalization of blue economy MSMEs in Bitung, Indonesia. The literature shows little research on digitalization related to the blue economy in Southeast Asia, especially in Indonesia. This indicates that there is a large research gap related to digitalization and the blue economy in the Indonesian context. Data was collected through the distribution of questionnaires with open-ended questions to blue economy MSMEs. Data was also obtained from in-depth interviews with representatives of Aruna, an Indonesian company that focuses on simplifying the supply chain of fishery products by connecting small-scale fishers to the global market through technology. According to the study's findings, two primary factors-motivation to develop their business and efforts to maintain seller-buyer interaction-support SMEs' use of technology in the blue economy. However, digital literacy and technological infrastructure, such as the internet network, are the two main factors that become obstacles in the effort to digitize MSMEs in the blue economy. The role of the government is also a contingent factor that can strengthen the relationship between factors that support digitization and weaken the relationship between factors that hinder digitalization.

• Journal of The Geomorphological Association of Korea
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• v.25 no.4
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• pp.103-116
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• 2018

The purposes of this research are as follows: First, to reconstruct the optimal locational environment of the Bronze Age settlement in Cheonan Baekseok-dong (hereinafter referred to as the study settlement). Second, to identify the priority ranking of the topographical factors that influenced the Bronze Age people when selecting their the settlement location. For these reasons, the topographical factors of the 200 Bronze Age dwellings (hereinafter referred to as dwellings) confirmed in the survey area were analyzed through the ridge's environmental perspective. The results are asfollows: First, the optimal ridge environment of the Bronze Age settlement is largely a sub-ridge in N-S direction (for example, NNW-SSE, N-S, NNESSW), especially at the top (Crest slope, Crest flat) of the south-facing aspect of the ridge. Second, when the Bronze Age dwellers selected a residential location, they carefully considered topographical factors in the following order: (1) the slope direction of the ridge surface, (2) the micro-landform of the ridge, (3) the ridge scale, (4) the ridge direction, and (5) the surface relief. The results of this study could be used as basic data in related fields such as archaeology, quaternary research, and traditional architecture and so on.

• Coupled systems mechanics
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• v.10 no.1
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• pp.39-60
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• 2021

The present paper is concerned with the study of nonlinear ultrasonic waves in a magneto thermo (MT) elastic armchair single-walled carbon nanotube (ASWCNT) resting on polymer matrix. The analytical formulation is developed based on Eringen's nonlocal elasticity theory to account small scale effect. After developing the formal solution of the mathematical model consisting of partial differential equations, the frequency equations have been analyzed numerically by using the nonlinear foundations supported by Winkler-Pasternak model. The solution is obtained by ultrasonic wave dispersion relations. Parametric work is carried out to scrutinize the influence of the non local scaling, magneto-mechanical loadings, foundation parameters, various boundary condition and length on the dimensionless frequency of nanotube. It is noticed that the boundary conditions, nonlocal parameter, and tube geometrical parameters have significant effects on dimensionless frequency of nano tubes. The results presented in this study can provide mechanism for the study and design of the nano devices like component of nano oscillators, micro wave absorbing, nano-electron technology and nano-electro- magneto-mechanical systems (NEMMS) that make use of the wave propagation properties of armchair single-walled carbon nanotubes embedded on polymer matrix.

• Journal of the Korean Society of Visualization
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• v.19 no.3
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• pp.39-45
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• 2021

An experiment to visualize fine dust captured by FOG droplet is conducted. Coal dust with 23.56 MMD (Mean Median Diameter) and water with 17.02 MMD is used as fine dust and FOG droplet. Long distance microscope and high-speed camera are used to capture the images of micro-scale particles sprinkled by acrylic duct. After measuring and comparing the size of the coal dust and FOG droplet to MMD, process to seize the coal dust with FOG droplet is recorded in 2 conditions: Fixed and Floated coal dust in the floated FOG droplet flow. In both conditions, a coal dust particle is collided and captured by a FOG droplet particle. A FOG droplet particle attached at the surface of the coal dust particle does not break and remains spherical shape due to surface tension. Combined particles are rotated by momentum of the particle and fallen.

• Current Optics and Photonics
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• v.6 no.4
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• pp.381-391
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• 2022

Two-photon-polymerization additive-manufacturing systems feature high resolution and precision. However, there are few reports on specific methods and possible problems concerning the use of small lasers to independently build such platforms. In this paper, a femtosecond-laser two-photon-polymerization additive-manufacturing system containing an optical unit, control unit, monitoring unit, and testing unit is built using a miniature femtosecond laser, with a detailed building process and corresponding control software that is developed independently. This system has integrated functions of light-spot detection, interface searching, micro-/nanomanufacturing, and performance testing. In addition, possible problems in the processes of platform establishment, resin preparation, and actual polymerization for two-photon-polymerization additive manufacturing are explained specifically, and the causes of these problems analyzed. Moreover, the impacts of different power levels and scanning speeds on the degree of polymerization are compared, and the influence of the magnification of the object lens on the linewidth is analyzed in detail. A qualitative analysis model is established, and the concepts of the threshold broadening and focus narrowing effects are proposed, with their influences and cooperative relation discussed. Besides, a linear structure with micrometer accuracy is manufactured at the millimeter scale.