• 제목/요약/키워드: Micro-organisms

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유용미생물을 이용한 포러스콘크리트의 수질정화특성에 대한 현장평가 (Site Assessment for the Water Purification Effect of Porous Concrete by using Effective Micro-organisms)

  • 김봉균;박준석;서대석;김화중;김윤용
    • 한국구조물진단유지관리공학회 논문집
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    • 제19권1호
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    • pp.109-119
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    • 2015
  • 최근 수십년간의 산업발달 및 인구증가에 따라 산업폐수 및 각종 비점오염원의 유입으로 수질오염이 심각한 실정이다. 이에 대해 국내 및 국외에서 이에 대한 연구가 활발히 진행되고 있다. 본 연구에서는 유기물질의 분해효과가 있는 유용미생물과 포러스콘크리트를 이용하여 하천 현장에 설치하여 구조적 성능평가와 장기간 수질정화능력을 평가하였다. 구조적 성능평가 결과, 시험체의 공극률은 15%가 적절하다고 판단되며, 또한 수질정화성능 검토 결과 각항목에 대한 제거율은 SS 34.1%, BOD 14.6%, COD 34.9%, T-N 11.4%, T-P 12.6%를 나타냈다. 따라서 유용미생물을 이용한 포러스콘크리트의 현장적용시 수질을 정화시키는데 효과적이라고 판단된다.

EFFECT OF IMMUNOPOTENTIATING AGENTS ON SUBCLINICAL MASTITIS IN CATTLE AND BUFFALOES

  • Chishti, M.A.;Afzal, M.;Muneer, R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제5권4호
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    • pp.733-736
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    • 1992
  • Different parameters of subclinical mastitis were compared in 327 cattle and 493 buffaloes and the effect of immunopotentiating agents on subclinical mastitis in these animals was studied. Subclinical mastitis was detected in 8.2 percent buffalo and 24.0 percent cow quarter by modified whiteside test (MWT). In both the species there was decrease in lactose contents with increase in the degree of MWT reactivity i.e. $4.8{\pm}1.14$ to $2.31{\pm}0.82$ in cattle and $5.01{\pm}1.47$ to $2.36{\pm}1.02$ in buffaloes. While the chloride contents of the milk increased with increase in the MWT reactivity i.e. $0.19{\pm}0.4$ to $0.30{\pm}0.06$ in cattle and $0.20{\pm}0.04$ to $0.31{\pm}0.13$ in buffaloes. Micro-organisms belonging to Staphylococcus, Micrococcus, Bacillus, Streptococcus, Enterobacteria, Corynebacterium groups and yeasts were isolated from subclinical mastitis cases. Vitamin E and Levamisole cured 64.5 and 60.0 percent cases of subclinical mastitis in buffaloes but only 32.0 and 24.0 percent cases in cattle. Cure was not affected by the degree of MWT and the type of organisms involved.

Biofouling and Microbial Induced Corrosion -A Case Study

  • Mohammed, R.A.;Helal, A.M.;Sabah, N.
    • Corrosion Science and Technology
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    • 제7권1호
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    • pp.27-34
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    • 2008
  • In industrial and fluid handling systems, frequently the protective film forming materials suffer from severe corrosion due to microbial effects. As an example, various micro-organisms, including bacteria, exist in seawater normally fed to power and desalination plants. Unless seawater intakes are properly disinfected to control these microbial organisms, biological fouling and microbial induced corrosion (MIC) will be developed. This problem could destroy metallic alloys used for plant construction. Seawater intakes of cogeneration plants are usually disinfected by chlorine gas or sodium hypochlorite solution. The dose of disinfectant is designed according to the level of contamination of the open seawater in the vicinity of the plant intake. Higher temperature levels, lower pH, reduced flow velocity and oxidation potential play an important role in the enhancement of microbial induced corrosion and bio-fouling. This paper describes, in brief, the different types of bacteria, mechanisms of microbiological induced corrosion, susceptibility of different metal alloys to MIC and possible solutions for mitigating this problem in industry. A case study is presented for the power plant steam condenser at Al-Taweelah B-station in Abu Dhabi. The study demonstrates resistance of Titanium tubes to MIC.

Comparison of the Nutritional Value of Chlorella ellipsoidea and Nannochloris oculata for Rotifers and Artemia Nauplii

  • Cabrera Tomas;Bae Jean Hee;Bai Sungchul C.;Hur Sung Bum
    • Fisheries and Aquatic Sciences
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    • 제8권4호
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    • pp.201-206
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    • 2005
  • Microalgae are widely used for mass culture of the rotifer Brachionus plicatilis in aquaculture. Since the nutritional value of the rotifer is closely related to its food, the nutritional value of its food should be known in detail. Chlorella ellipsoidea and Nannochloris oculata are re­presentative food organisms for rotifers that are easily cultured. Therefore, the nutritional values of these micro algae were examined for ultrasmall, small, and large rotifers and Artemia nauplii. Chlorella ellipsoidea contained seven times more total fatty acids than N. oculata. The three types of rotifer fed N. oculata contained more amino acids than those fed C. ellipsoidea. However, the total fatty acids of the rotifers fed each microalga species differed according to the type of rotifer. Newly hatched Artemia nauplii contained more protein and had a higher dry weight than those fed microalgae for 6 h. As with the rotifers, the Artemia nauplii fed N. oculata contained more protein and amino acids than those fed C. ellipsoidea, while the reverse was true for the total fatty acid content. Our results suggest that N. oculata is a good supply of protein, while C. ellipsoidea is a good source of lipids as food organisms for rotifers and Artemia nauplii in aquaculture.

인체 피부에 기생한 Leishmania tropica의 전자현미경적 관찰 (Ultrastructural Observations of a Human Cutaneous Leishmaniasis)

  • 서영훈;허규정;등영건;김정숙;이유복
    • Applied Microscopy
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    • 제10권1_2호
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    • pp.27-32
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    • 1980
  • 중동지방에 다녀온 48세의 한국인 남자 피부에 발생한 leishmania증 1예에 대하여 광학 및 전자현미경적 관찰을 하여 문헌 고찰과 아울러 보고하였다. 광학현미경적으로는 조직구 침윤을 주로 하는 만성 육아종성염증반응을 보였고 Giemsa염색상 다수의 leishmania충체를 조직구내에서 관찰할 수 있었다. 전자현미경적으로는 난원형의 단세포 또는 분열중인 충체가 조직구내에 존재하였고 충체는 이중막으로 싸였으며 그 바로밑에 microtubule이 배열되고 세포질내에는 신장된 mitochondrion내에 kinetoplast가 존재하고 그 전방에 flagella가 위치하였으며 기타 다수의 ribosome과 드물게 Golgi complex등을 관찰할 수 있었다. 이와같은 특징은 Leishmania tropica의 promastigote stage와 일치하였다. Leishmania증은 원칙적으로 열대병이나 열대지방과의 접촉이 빈번함에 따라 앞으로 우리나라에서도 보다 많은 증예가 발생할 것으로 사료된다.

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인조기판 위에서 해양 부착생물의 발달 및 천이 (Development and Succession of Marine Fouling Organisms on Artificial Substrata)

  • 심재형;정문섭
    • 한국해양학회지
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    • 제22권4호
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    • pp.257-270
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    • 1987
  • 1982-1983년 진해항 및 마산만에서 발달하는 부착생물 군집을 슬라이드 글라스 및 浸漬基板실험에 의하여 연구하였다. 15일의 침적실험후 세균의 개체수는 1.7$\times$$10^{4}$CFU/$cm^{2}$로 산출되었고, 微小生物群集에서 발달한 저생 규조류 는 총 46분류군이었다. 부착생물군집의 順次的 변화과정을 명백히 볼 수 있었고, 우 점 규조류 종은 Licmophora flavellata, Navicula grevillei, 및 Nitzschia closterium 로 나타났다. 대형생물군집의 주요 종은 Mytilus edulis, Balanus amphitrite mphitrite, Hydroides ezoensis및Celleporina sp. 로서 5개월의 침적후에 500g/100cm $^{2}$이상의 생물량을 보였다. 두 조사해역에서의 군집발달양상이 큰 차이를 보이 는데 이는 주로 주변수의 물리화학적 안정성 및 영양상태에 기인하는 것으로 판단 된다. 부착생물군집의 발달과정은 유생의 생성시기 및 성장률에 매우 큰 영향을 받 는 것으로 밝혀졌고, 군집발달의 전 과정은 다음과 같이 요약이 된다. 세균 및 규조류-다세포해조류-따개비류, 홍합류 및 다모류-해면류, 말미잘류 및 해초류.

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Bioinspired Metal Surfaces with Extreme Wettability Contrast

  • 유의선;허은규;고태준;이광렬;오규환;문명운
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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    • pp.122-122
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    • 2012
  • The exterior structures of natural organisms have continuously evolved by controlling wettability, such as the Namib Desert beetle, whose back has hydrophilic/hydrophobic contrast for water harvesting by mist condensation in dry desert environments, and some plant leaves that have hierarchical micro/nanostructures to collect or repel liquid water. In this work, we have provided a method for wettability contrast on metals by both nano-flake or needle patterns and tuning of the surface energy. Metals including steel alloys and aluminum were provided with hierarchical micro/nanostructures of metaloxides induced by fluorination and a subsequent catalytic reaction of fluorine ions on metal surfaces in water with various ranges from room to boiling temperature of water. Then, a hydrophobic material was deposited on the structured surfaces, rendering superhydrophobicity. Plasma oxidization induces the formation of superhydrophilic surfaces on selective regions surrounded by superhydrophobic surfaces. We show that wettability contrast surfaces align liquid water within patterned hydrophilic regions during the condensation process. Furthermore, this method could have a greater potential to align other liquids or living cells.

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플래핑 운동을 적용한 자율무인잠수정(AUV)의 날개형상 및 운동 최적설계 (Optimization Design of Hydrofoil Shape and Flapping Motion in AUV(Autonomous Underwater Vehicle))

  • 김일환;최중선;박경현;이도형
    • 한국유체기계학회 논문집
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    • 제16권1호
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    • pp.24-31
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    • 2013
  • The motion of living organisms such as birds, fishes, and insects, has been analyzed for the purpose of the design of MAV(Micro Air Vehicle) and NAV(Nano Air Vehicle). In this research, natural motion was considered to be applied to the determination of the geometry and motion of AUV(Autonomous Underwater Vehicle). The flapping motion of a number of hydrofoil shapes in AUV was studied, and at the same time, the optimization of the hydrofoil shape and flapping motion was executed that allow the highest thrust and efficiency. The harmonic motion of plunging and pitching of NACA 4 digit series models, was used for the numerical analysis. The meta model was made by using the kriging method in Optimization method and the experimental points of 49 were extracted for the OA(Orthogonal array) in DOE(Design of experiments). Parametric study using this experimental points was conducted and the results were applied to MGA(Micro Genetic Algorithm). The flow simulation model was validated to be an appropriate tool by comparing with experimental data and the optimized shape and motion of AUV was turned out to produce highest thrust and efficiency.

Estimation of micro-biota in the Upo wetland using eukaryotic barcode molecular markers

  • Park, Hyun-Chul;Bae, Chang-Hwan;Jun, Ju-Min;Kwak, Myoung-Hai
    • Journal of Ecology and Environment
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    • 제34권3호
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    • pp.323-331
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    • 2011
  • Biodiversity and the community composition of micro-eukaryotic organisms were investigated in the Upo wetland in Korea using molecular analysis. Molecular identification was performed using cytochrome oxidase I (COI) and small subunit ribosomal DNA (SSU rDNA). The genomic DNA was isolated directly from soil samples. The COI and SSU rDNA regions were amplified using universal primers and then sequenced after cloning. In a similarity search of the obtained sequences with BLAST in the Genbank database, the closely related sequences from NCBI were used to identify the amplified sequences. A total of six eukaryotic groups (Annelida, Arthropoda, Rotifera, Chlorophyta, Bacillariophyta, and Stramenopiles) with COI and six groups (Annelida, Arthropoda, Rotifera, Alveolata, Fungi, and Apicomplexa) with SSU rDNA genes were determined in the Upo wetland. Among 38 taxa in 20 genera, which are closely related to the amplified sequences, 10 genera (50%) were newly reported in Korea and five genera (25%) were shown to be distributed in the Upo wetland. This approach is applicable to the development of an efficient method for monitoring biodiversity without traditional taxonomic processes and is expected to produce more accurate results in depositing molecular barcode data in the near future.

밸러스트 처리수 미생물 모니터링을 위한 Sampling Device 개발 (Development of sampling device for monitoring micro-organisms in treated ballast water)

  • 박성진;김기욱;윤승제;조동연;김상용
    • 한국마린엔지니어링학회:학술대회논문집
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    • 한국마린엔지니어링학회 2011년도 전기공동학술대회 논문집
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    • pp.312-312
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    • 2011
  • IMO BWM 협약이 발효가 되면 선박에 Ballast 수 처리 시스템 탑재 후 국가 간 항구 도착 시 각주관청의 Ballast water Discharge regulation D-2 만족 여부를 검사받게 된다. Ballast Water Sampling Device는 이러한 D-2 규정에서 요구하는 많은 양의 시료를 농축 후 신속히 분석하여 DATA의 신뢰성을 확보하기 위한 시료 전처리 장비로써, 그 활용성이 매우 클 것으로 판단된다. 본 연구 개발을 통해 개발된 Sampling Device의 특징은 많은 양의 시료를 효율적으로 농축하는 농축조와 농축 후 플랑크톤 네트에 잔류하는 미생물을 회수하는 린싱조로 구성되며, 다양한 실검증을 통해 최적의 조건을 확립하였다. 또한 모든 Sampling 공정이 자동화로 구현됨으로서 선박에 적합한 효율적인 Operating이 이루어질 수 있도록 구성하였다.

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