• 제목/요약/키워드: Micro mineral

검색결과 252건 처리시간 0.023초

Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry

  • Kim, Jia;Song, Young-Sang;Min, Kyung-San;Kim, Sun-Hun;Koh, Jeong-Tae;Lee, Bin-Na;Chang, Hoon-Sang;Hwang, In-Nam;Oh, Won-Mann;Hwang, Yun-Chan
    • Restorative Dentistry and Endodontics
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    • 제41권1호
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    • pp.29-36
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    • 2016
  • Objectives: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.

In vivo micro-CT를 이용한 난소적출 백서의 골구조 형태계측학적 분석 (Morphometric analysis of bone in the ovariectomized rat using in vivo micro-CT)

  • 이창진;이완;이병도
    • Imaging Science in Dentistry
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    • 제38권1호
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    • pp.29-37
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    • 2008
  • Purpose: The aim of this study was to observe the bony change in the OVX rat longitudinally and to study the alendronate effect. Materials and Methods: Eighteen Sprague-Dewley rats, eight-week old each, were randomly assigned into three groups: one of those sham-operated (N=4), the other two were OVX: saline-treated (N=7) and alendronate-treated group (N=7). The saline-treated group was administered with saline solution (0.1mL/100g) daily, while the alendronate-treated group was given alendronate (1mg/kg, Sigma-Aldrich Corp. Korea) daily. Micro-CT scannings of the lumbar were consecutively done at baseline, at 3-week intervals during 9 weeks. Two and three dimensional bony analysis were done. Bone mineral density (BMD) was measured with Piximus (GE Lunar Co. USA). The average values of these three methods were compared with each group. Results: After 6 weeks the BMD of the OVX group showed lower tendency than that of sham group. After 6 weeks many 3D parameters of micro-CT showed higher values in the OVX-alendronate group compared with the OVXsaline group. Most 2D bony parameters were higher in the OVX-alendronate group compared with the OVX-saline group at 9 weeks. Conclusion: This study showed low BMD of the OVX group after 6 weeks and showed the effect of alendronate on the BMD and bony structures of ovariectomized rats. This study also showed usefulness of in vivo micro-CT in monitoring individual bone changes over time.

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A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials

  • Fernanda Ferrari Esteves Torres;Jader Camilo Pinto;Gabriella Oliveira Figueira;Juliane Maria Guerreiro-Tanomaru;Mario Tanomaru-Filho
    • Restorative Dentistry and Endodontics
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    • 제46권1호
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    • pp.2.1-2.8
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    • 2021
  • Objectives: New premixed bioceramic root repair materials require moisture for setting. Using micro-computed tomography (micro-CT), this study evaluated the filling ability and volumetric changes of calcium silicate-based repair materials (mineral trioxide aggregate repair high-plasticity [MTA HP] and Bio-C Repair, Angelus), in comparison with a zinc oxide and eugenol-based material (intermediate restorative material [IRM]; Dentsply DeTrey). Materials and Methods: Gypsum models with cavities 3 mm deep and 1 mm in diameter were manufactured and scanned using micro-CT (SkyScan 1272. Bruker). The cavities were filled with the cements and scanned again to evaluate their filling capacity. Another scan was performed after immersing the samples in distilled water for 7 days to assess the volumetric changes of the cements. The statistical significance of differences in the data was evaluated using analysis of variance and the Tukey test with a 5% significance level. Results: Bio-C Repair had a greater filling ability than MTA HP (p < 0.05). IRM was similar to Bio-C and MTA HP (p > 0.05). MTA HP presented the largest volumetric change (p < 0.05), showing more volume loss than Bio-C and IRM, which were similar (p > 0.05). Conclusions: Bio-C Repair is a new endodontic material with excellent filling capacity and low volumetric change. The gypsum model proposed for evaluating filling ability and volumetric changes by micro-CT had appropriate and reproducible results. This model may enhance the physicochemical evaluation of premixed bioceramic materials, which need moisture for setting.

경기도, 강원도, 충청도 일대의 암석 열물성 특성 연구 (A Study on Thermal Properties of Rocks from Gyeonggi-do Gangwon-do, Chungchung-do, Korea)

  • 박정민;김형찬;이영민;송무영
    • 자원환경지질
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    • 제40권6호
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    • pp.761-769
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    • 2007
  • 경기도, 강원도, 충청도 일대에서 화성암, 변성암, 퇴적암의 총 712개의 암석을 채취하여 열물성을 측정하였다. 측정 결과로 화성암의 평균 열전도도는 3.58W/m-K, 변성암은 4.16W/m-K, 퇴적암은 4.53W/m-K이다. 우리나라의 경우 화강암과 편마암이 주를 이루고 있는데 이에 대한 열물성 값을 보면 화강암의 열전도도는 2.13-5.87W/m-K의 범위를 가지며, 평균 열전도도는 3.57W/m-K, 편마암은 2.26-6.67W/m-K의 범위를 가지며, 평균 열전도도는 3.945W/m-K이다. 화강암의 평균 열확산율은 $1.43mm^2/sec$, 비열은 0.914J/gK, 편마암의 평균 열확산율은 $1.55mm^2/sec$, 비열은 0.912J/gK로 나타났다. 일반적으로 같은 암석의 열전도도 값의 범위가 큰 이유는 암석의 구성광물, 이방성 등에 영향을 받기 때문이다.

Chemical Properties of Indoor Individual Particles Collected at the Daily Behavior Spaces of a Factory Worker

  • Ma, Chang-Jin;Kang, Gong-Unn;Sakai, Takuro
    • Asian Journal of Atmospheric Environment
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    • 제11권2호
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    • pp.122-130
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    • 2017
  • The main purpose of the study was to clarify the properties of individual particles collected at each behavior space of a factory worker. The samplings of size-segregated ($PM_{2.1-1.1}$ and $PM_{4.7-3.3}$) indoor particles were conducted at three different behavior spaces of a factory worker who is engaged in an auto parts manufacturing plant (i.e., his home, his work place in factory, and his favorite restaurant). Elemental specification (i.e., relative elemental content and distribution in and/or on individual particles) was performed by a micro-PIXE system. Every element detected from the coarse particulate matters of home was classified into three groups, i.e., a group of high net-counts (Na, Al, and Si), a group of intermediate net-counts (Mg, S, Cl, K, and Ca), and a group of minor trace elements (P, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, and Pb). The results of EF for $PM_{4.7-3.3}$ in home indicated that several heavy metals were generated from the sources within the house itself. An exceptional feature shown in the individual particles in workplace is that Cr, Mn, and Co were clearly detected in both fine and coarse particles. Cluster analysis suggested that the individual coarse particles ($PM_{4.7-3.3}$) collected at the indoor of factory were chemically heterogeneous and they modified with sea-salt, mineral, and artificially derived elements. The principal components in individual coarse particles collected at restaurant were sea-salt and mineral without mixing with harmful trace elements like chromium and manganese. Compared to the indoor fine particles of home and restaurant, many elements, especially, Cl, Na, Cr, Mn, Pb, and Zn showed overwhelmingly high net-counts in those of factory.

투과전자현미경분석용 박편 제작 시 집속이온빔에 의한 광물 손상 (Damage of Minerals in the Preparation of Thin Slice Using Focused Ion Beam for Transmission Electron Microscopy)

  • 정기영
    • 한국광물학회지
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    • 제28권4호
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    • pp.293-297
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    • 2015
  • 집속이온빔(FIB, focused ion beam)법은 광물 및 지질시료의 분석 대상 위치로부터 투과전자현미경(TEM, transmission electron microscope) 관찰을 위한 박편을 정밀하게 제작할 수 있는 방법으로 널리 보급되고 있다. 그러나 박편 제작과정에서 Ga 이온빔에 의한 구조 손상이나 인위적 효과들이 발생하여 전자빔에 의한 손상과 함께 TEM 분석에서의 난점들 중 하나이다. 광물 시료 FIB 박편의 TEM 관찰에서 석영과 장석의 비정질화, 커튼 효과, Ga 오염 등이 확인되었으며, 특히 입자 경계 부근이나 두께가 얇은 곳에서 이들 현상이 보다 뚜렷하다. 박편 제작 시의 가속전압 및 전류 조정 등의 분석절차 개선으로 이온빔 손상을 줄일 수 있으나, 어느 정도의 손상이나 오염은 피할 수 없으므로 TEM 박편 관찰과 해석에서 유의하여야 한다.

순환식 수경재배시 무기이온 조절이 Single-Node Cutting 장미의 양분흡수, 생육 및 품질에 미치는 영향 (Effect of Mineral Nutrient Control on Nutrient Uptake, Growth and Yield of Single-Node Cutting Rose Grown in a Closed Hydroponic System)

  • 양은영;박금순;오정심;이혜진;이용범
    • 생물환경조절학회지
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    • 제17권4호
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    • pp.252-260
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    • 2008
  • 본 실험은 장미 식물공장에서 single-node cutting 'Versillia'의 양분흡수 특성을 구명하고 순환식 수경재배 시스템에 적합한 배양액 내 무기이온 조절방식을 구명하고자 수행하였다. 실험 기간동안 각 처리별 배양액 내 무기이온함량 변화를 살펴본 결과 EC 제어구의 경우 $NO_3$-N은 생육 후반이 되면 그 함량이 적정 범위 이상으로 증가 하였고 P와 Mg은 감소하였으며 배양액 첨가구는 전체적인 배양액 내 무기이온 함량이 증가하였다. 이에 비해 무기이온 제어구는 생육 기간동안 근권 내 적정 범위를 유지하였다. 광합성 효율을 나타내는 지표 중 하나인 Fv/Fm는 무기이온제어구와 배양액 첨가구에서 높았고 절화장, 생체중 등은 무기이온 제어구에서 높았다. 그러나 뿌리의 활성 정도를 측정해본 결과 무기이온 제어구에서 높았으나 배양액 첨 가구에서는 생육 초기에 배양액 내 pH 변화폭이 커서 가장 낮게 나타났다. 위의 결과를 종합하였을 때, 무기이온 조절(다량 미량원소 제어구와 다량원소 제어구)을 통해 배양액을 관리하는 것이 생육 전 기간 동안 근권 환경을 적절히 유지해 줄 수 있었고 이에 따라 절화 품질도 향상되므로 장미(single-node cutting) 순환식 수경재배에 적합한 근권 환경 제어방식이라고 할 수 있다.

Hydroxyapatite를 이식한 임플란트 주위 골결손부에서 rhPDGF-BB와 rhBMP-2가 골내 임플란트 osseointegration에 미치는 영향: Micro-CT 분석과 조직학적 평가 (EFFECT OF RHPDGF-BB AND RHBMP-2 ON OSSEOINTEGRATION OF TITANIUM IMPLANTS AT PERIIMPLANT BONE DEFECTS GRAFTED WITH HYDROXYAPATITE: MICRO-CT AND HISTOLOGIC ANALYSIS)

  • 박지현;황순정;김명진
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제31권6호
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    • pp.461-468
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    • 2009
  • Purpose: Platelet derived growth factor(PDGF)-BB and bone morphogenetic protein(BMP)-2 are well-known representative growth factors. The purposes of this study were to investigate the effect of rhPDGFBB and rhBMP-2 on osseointegration of titanium implants at periimplant bone defects grafted with hydroxyapatite and to evaluate the feasibility of imaging bone structures around screw-type titanium implant with micro-CT. Materials and Methods: The first molar and all premolars in the mandible region of four beagle dogs were extracted. Following a healing period of 4 months, three $8{\times}8{\times}6mm$-sized bony defects were formed and screw-type titanium implants were placed with hydroxyapatite(HA) block and growth factors; Control group, PDGF group and BMP group. Two months post-implantation, the mandible was harvested. Bone volume(BV), bone-to-implant contact(BIC) and bone mineral density(BMD) were analyzed with micro-CT and histology. Results: According to micro-CT analysis, BV and BMD measures of PDGF and BMP group were significantly higher than control group(BV; PDGF group: $p{\fallingdotseq}0.011$, BMP group: $p{\fallingdotseq}0.006$/BMD; PDGF group: $p{\fallingdotseq}0.020$, BMP group: $p{\fallingdotseq}0.011$) and BIC measures of BMP group were significantly higher than PDGF group($p{\fallingdotseq}0.015$). In histologic evaluation, BIC measures of BMP group was significantly higher than PDGF group($p{\fallingdotseq}0.048$). The values of BV in histologic sections were higher than in micro-CT images and the values of BIC in micro-CT images were higher than in histologic sections. Conclusion: The findings of this experimental study indicates that the use of rhPDGF-BB and rhBMP-2 can increase new bone formation in a large bony defect around titanium implant, and rhBMP-2 is more effective than rhPDGF-BB. Micro-CT can be considered useful for assessment as a rapid and nondestructive method for 3-dimensional measurement of bone healing around implants. Further study is necessary, however, to remove metal artifacts around titanium implant and to standardize the method.

Chitin-fibroin-hydroxyapatite membrane for guided bone regeneration: micro-computed tomography evaluation in a rat model

  • Baek, Young-jae;Kim, Jung-Han;Song, Jae-Min;Yoon, Sang-Yong;Kim, Hong-Sung;Shin, Sang-Hun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제38권
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    • pp.14.1-14.6
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    • 2016
  • Background: In guided bone regeneration (GBR) technique, many materials have been used for improving biological effectiveness by adding on membranes. The new membrane which was constructed with chitin-fibroin-hydroxyapatite (CNF/HAP) was compared with a collagen membrane (Bio-$Gide^{(R)}$) by means of micro-computed tomography. Methods: Fifty-four rats were used in this study. A critical-sized (8 mm) bony defect was created in the calvaria with a trephine bur. The CNF/HAP membrane was prepared by thermally induced phase separation. In the experimental group (n = 18), the CNF/HAP membrane was used to cover the bony defect, and in the control group (n = 18), a resorbable collagen membrane (Bio-$Gide^{(R)}$) was used. In the negative control group (n = 18), no membrane was used. In each group, six animals were euthanized at 2, 4, and 8 weeks after surgery. The specimens were analyzed using micro-CT. Results: Bone volume (BV) and bone mineral density (BMD) of the new bone showed significant difference between the negative control group and membrane groups (P < 0.05). However, between two membranes, the difference was not significant. Conclusions: The CNF/HAP membrane has significant effect on the new bone formation and has the potential to be applied for guided bone regeneration.

마이크로 휜 증발관내 냉매 R-290의 열전달 특성에 미치는 냉동유의 영향 (Influence of Refrigeration Oil on Evaporation Heat Transfer Characteristics of R-290 Inside Micro Fin Tube)

  • 박철민;안영태;이욱현;김정훈;김종수
    • 대한기계학회논문집B
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    • 제24권7호
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    • pp.938-944
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    • 2000
  • Recently, micro fin tube is widely used to heat exchanger for high performance. And, as the alternative refrigerants for R-22, hydrocarbons such as R-290, R-600 and R-600a are very promising because of their low GWP and ODP. Thus, R-290 was used as working fluid in this study. Most design of heat exchanger had been based on heat transfer characteristics of pure refrigerant although refrigerant oil exists in the refrigeration cycles. So, the influence of oil on heat transfer characteristics have to be considered for investigating exact evaporation heat transfer characteristics. But, this is an unresolved problem of refrigeration heat transfer. Therefore the influence of the refrigeration oil to the evaporation heat transfer characteristics of R-290 were conducted in a horizontal micro tin tube. The mineral oil was used as refrigeration oil. The experimental apparatus consisted of a basic refrigeration cycle and a system for oil concentration measurement. Test conditions are as the follows; evaporation temperature $5^{\circ}C$, mass velocity 100 $kg/m^2s$, heat flux 10 $kW/m^2$, oil concentration 0, 1.3, 3.3, 5.7 wt.%, and quality $0.07{\sim}1.0$. When refrigeration oil was entered, oil foaming was observed at the low quality region. And, very small bubbles were observed as quality was increased. Pressure drop and heat transfer coefficient increased as the concentration of refrigeration oil increased to 5 wt.%.. The performance index of heat exchanger was the highest near 3.3 wt.%.