• 한국임상수의학회지
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• 제39권6호
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• pp.302-310
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• 2022

Recently, in human medicine and veterinary medicine, interest in synthetic bone graft is increasing. Among them, bone morphogenic protein (BMP) is currently being actively researched and applied to clinical trials. However, BMP has the disadvantage of being expensive and easily absorbed into surrounding tissues. Therefore, BMP requires the use of small amounts and rhBMP (recombinant human bone morphogenetic protein)-2 carriers that can be released slowly. Hydrogel has the property of swelling a large amount of water inside when it is aqueous solution, and when it is, it consists of more than 90 percent water. Using these properties, hydrogels are often used as rhBMP-2 carrier. The scaffold used in this study is a hydrogel made from which keratin is extracted using human hair and based on it. In this study, we wanted to see the effect of bone formation in the calvarial defect model by using keratin-based hydrogel made with human hair as a scaffold. The experiment was conducted by dividing 3 groups a total of 12 mice. Calvarial bone defect is set to all 4 mm diameters. Bone formation was evaluated by using gross evaluation, micro-computed tomography (micro-CT), immunohistochemistry. Groups using keratin-based hydrogel were significantly observed compared to Group 1s, and the most bone formations were found when rhBMP-2 and hydrogel were used. This represents the superiority of the functions of the rhBMP-2 carrier by a new material, keratin-based hydrogel. Through gross evaluation, micro-CT, and immunohistochemistry, we can confirm that keratin-based hydrogel is a useful rhBMP-2 carrier.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1075-1079
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• 2014

MicroRNA expression is a research focus in studies of tumors. This article concentrates attention on potential links between tumors caused by mouse mammary tumor virus (MMTV) and human breast cancer, in order to provide theoretical basis for using mouse model to search for miRNA effects mediated by Wnt/beta-catenin signaling in human breast cancer. By analyzing interactions between miRNAs and the Wnt/beta-catenin signaling pathway in breast cancer, we hope to casts light on more biological functions of miRNAs in the process of tumor formation and growth and to explore their potential value in cancer diagnosis, prognosis and treatment. Our endeavor aimed at providing theoretical basis for finding safer, more effective methods for treatment of human breast cancer at the miRNA molecular level.

• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.142-150
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• 2007

Objectives : This study was carried out to investigate the latest study tendency in mouse model of skin pruritus published since 2005 and to arrange various experimental methods. Methods : We examined the journal(such as Experimental dermatology and British journal of dermatology) and in Pubmed since 2005, regarded pruritus and scratching behavior model as key words. Results and Conclusion : 1. BALB/c, hairless, NC/Nga and ICR mice were the most used in scratching behavior model. According to scratching-induced agents. we need to select experimental mice. 2. There are various methods inducing skin pruritus; by cohabitation with NC/Nga mice having severe skin lesions, by injection intradermally(or subcutaneously) with agent inducing inflammation, by inducing contact dermatitis with TNCB, TNFB, destruction of skin barrier and by transgenic mice. 3. Injection intradermally(or subcutaneously) with agent inducing inflammation is the most used out of methods inducing skin pruritus. Compound 48/80, histamine, substance P and others(chloroquine, serotonin, carrageenin, TPA etc.) were included in agents inducing inflammation and pruritus in skin pruritus model. 4. Video camera, SCLABA system, MicroAct and acoustic evaluation system were included in evaluation methods of scratching behavior mouse model.

• Clinical and Experimental Reproductive Medicine
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• 제1권1호
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• pp.49-54
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• 1974

In the present studies, effect of progesterone on the germinal vesicle break-down of the mouse oocytes cultured in the micro tube was investigated. The results obtained are as follows: As dose of progesterone in the medium rose, accordingly the break-down of the germinal vesicle was suppressed. It was found that $ED_{50}$ was 15.7 ${\mu}g$/ml, and $ED_{90}$ 60.7 ${\mu}g$/ml of progesterone. The dose suppressing the oocyte maturation was apparently higher than that on the rabbit or on the mouse embryonal development. The inhibiting effect of progesterone on the GVBD was reversible. The germinal vesicle of the oocytes were broken down immediately in the medium upon removal of the hormone. Progesterone stops meiosis at any stage upon administration, while dbe AMP or theophylline supresses only the break-down of the nuclear membrane. Recovering of the meiotic division of the oocytes once exposed to progesterone was delayed a little. The inhibiting action of progesterone was not altered by adding more pyruvate or in the presence of higher concentration of the mineral ions in the culture medium.

• Reproductive and Developmental Biology
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• 제32권4호
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• pp.229-235
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• 2008

Silencing of Dicer1 by siRNA did not inhibit development up to the blastocyst stage, but decreased expression of selected transcription factors, including Oct-4, Sox2 and Nanog, suggesting that Dicer1 gene expression is associated with differentiation processes at the blastocyst stage (Cui et al., 2007). In order to get insights into genes which may be linked with microRNA system, we compared gene expression profiles in Gapdh and Dicer1 siRNA-microinjected blastocysts using the Applied Biosystem microarray technology. Our data showed that 397 and 737 out of 16354 genes were up- and down-regulated, respectively, following siRNA microinjection (p<0.05), including 24 up- and 28 down-regulated transcription factors. Identification of genes that are preferentially expressed at particular Dicer1 knock down embryos provides insights into the complex gene regulatory networks that drive differentiation processes in embryos at blastocyst stage.

• Toxicological Research
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• 제14권2호
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• pp.211-216
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• 1998

In order to evaluate the mutagenic potential of SDK(skin decontamination kit) produced by Agency for Defense Development(ADD), were performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells according to the established regulation of Korean Food and Drug Administration. In the reverse mutation test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 did not in-crease the number of revertant at any of the concentration tested in this study. SDK did not increase the number of cells having structural or numerical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase in the occurrence oj micro nucleated polychromatic erythrocytes were observed in ICR male mice intraperitoneally administered with SDK. These results indicate that SDK has no mutagenic effects under these experimental conditions.

• 한국가축번식학회지
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• 제10권1호
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• pp.42-48
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• 1986

These experiments were carried out to develop new techniques identifying XX-bearing embryos prior to implantation by immunological method. Antiserum to histocompatibility-Y(H-Y) antigen was prepared in adult SD(sprague-dawley) female rat by repeated immunization of newbone testis supernatant from males of the same strain. ELISA test was used to identify the H-Y antibody of antiserum. Total 124 mouse embryos (8-cell stage) were treated with H-Y antiserum and complement in BSA free Ho, pp. and Pitt's medium and cultured under the gas phase of 5% CO2 in air at 37$^{\circ}C$ for 24 to 48 hrs. The morphological characteristics of embryos treated were observed under the phase-contrast micro scope. The results obtained in these experiments were summarized as follows: 1. Optimal Density of H-Y antibody were a, pp.ared to be 0.27-0.47 by ELISA test. 2. Of total 124 embryos treated with H-Y antiserum and complement 69(55.6%) embryos developed to blastocyst and 55(44.4%) destroyed or arrested.

• 비파괴검사학회지
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• 제25권4호
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• pp.239-246
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• 2005

골다공증에 따른 쥐 대퇴골의 고유진동수의 변화를 예측하기 위하여 유한요소해석과 진동 시험을 수행하였다. 연구 대상으로 골다공증에 걸린 쥐 그룹, 골다공증을 치료한 쥐 그룹, 그리고 골다공증이 없는 정상 쥐 그룹을 구성하였다. 유한요소해석에서는 미세단층촬영(Micro-CT)에서 얻은 해면골 영상 데이터와 Voxel 요소 생성 알고리즘을 이용하여 3차원 미세 유한요소 모델을 생성한 다음 고유진동수 해석을 수행하였다. 진동 시험에서는 모빌리티 실험에 의한 주파수 응답함수로부터 고유진동수를 측정하였다 연구 결과로부터 골다공증에 걸린 쥐 그룹의 고유진동수가 가장 높고, 골다공증을 치료한 쥐의 그룹, 골다공증이 없는 정상 쥐의 그룹 순서로 낮아지는 경향을 볼 수 있었다. 모든 그룹에서 유한요소해석에 의한 고유진동수는 시험에서 측정한 결과보다 10-15% 오차 범위 내로 높은 값을 보였다. 본 연구로부터 개발된 미세단층촬영기 장치, Voxel 요소 생성 알고리즘, 진동시험 및 유한요소해석 방법을 이용하여 골다공증에 의한 생체 골조직의 구조적 변화 진단 및 치료 평가 등에 대한 응용가능성을 확인할 수 있었다.

• 생물환경조절학회지
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• 제9권4호
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• pp.237-243
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• 2000

현재 온실에서 농작업을 작업기는 사람이 운전하고 있다. 온실 안은 무척이나 무덥고 열악한 작업조건이다. 그렇기 때문에 앞으로 무더운 온실내의 작업기 개발은 사람이 직접 운전하지 않고, 자동화에 의한 무인화 될 것으로 전망된다. 온실에서 자동화 및 무인화의 작업을 돕기 위하여 온실내의 경로(길)에 따라 자동적으로 이동할 수 있는 시스템을 개발하게 되었다. 이 시스템은 작업기의 자동화와 무인화를 위한 것이며, 이를 위하여 온실의 천장에 가이드를 연결한 후 이를 따라 시스템이 자동으로 이동할 수 있는 경로 자동변환 시스템이다. 이 시스템은 연마봉으로 만들어진 경로를 따라 선회 및 직선운동이 가능하며, 이 시스템은 경로 변경을 위한 리미트 스위치와 소프트웨어로 구성되어 있다. 이 시스템의 작동여부를 실험하기 위하여 시테핑모터를 가진 마이크로 마우스를 이용하였다. 견고한 실험실의 평면 위에서의 작동실험은 시스템 작동이 100% 성공률을 나타내었지만 모래위나 다른 조건에서는 성공률이 80% 이하로 낮게 나타났다. 실제 온실에서도 충분한 강도를 가진 연마봉을 이용하여 처짐에 대한 부분을 고려하여 사용한다면 경로를 따라 주행하는 이 시스템은 잘 작동할 수 있을 것으로 기대된다.

• Animal cells and systems
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• 제8권3호
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• pp.213-220
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• 2004

MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.