• Journal of the Korean Society for Nondestructive Testing
• /
• v.32 no.1
• /
• pp.20-26
• /
• 2012

The objective of this study is to assess plastic deformation in aluminium alloy by acoustic nonlinearity of laser-generated surface waves. A line-arrayed laser beam made by high-power pulsed laser and mask slits is utilized to generate the narrowband surface wave and the frequency characteristics of laser-generated surface waves are controlled by varying the slit opening width and slit interval of mask slits. Various degrees of tensile deformation were induced by interrupting the tensile tests so as to obtain aluminum specimens with different degrees of plastic deformation. The experimental results show that the acoustic nonlinear parameter of a laser-generated surface wave increased with the level of tensile deformation and it has a good correlation with the results of micro-Vickers hardness test and electron backscatter diffraction (EBSD) test. Consequently, acoustic nonlinearity of laser-generated surface wave could be potential to characterize plastic deformation of aluminum alloy.

• Proceedings of the KSR Conference
• /
• 2007.11a
• /
• pp.313-336
• /
• 2007

Unlike a conventional railway system, a high-speed rail system experiences various aerodynamic problems in tunnel sections. Trains running at a high speed in a small tunnel, when compared with the open field, face significant air pressure, resulting in reduced operating stability and fast change in pressure inside the tunnel. These phenomena further cause some unexpected problems such as the passengers onboard feeling an aural discomfort and an impulsive noise at the tunnel exit. To solve these problems, this paper introduces analysis of aerodynamic characteristics for determination of tunnel cross section. The optimum cross-section that satisfies the criteria of aural discomfort was reviewed through lots of numerical simulation analysis. Also, the pressure inside the passenger car of a train operating on Kyungbu HSR line was measured, and the pressure inside the tunnel and the micro-pressure waves at tunnel exit were measured at Hwashin 5 Tunnel. At the same time, a test of train operation model was performed and then the measurement results and test results were compared to verify that various parameters used as input conditions for the numerical simulations were appropriate.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.40 no.2
• /
• pp.70-77
• /
• 2003

In this paper, analysis and measurement on coaxial connecter designed for packaging of microwave and micro strip transition structure are carried out. Even though researches on optimization of various kinds of transition structures have been conducted actively; however, the range of the application was very limited since they have been focused mainly on improvement of specific transmission line. Therefore, in this paper, we tried to analyze three kinds of substrates of which dielectric constants are 2,5,10 and are commercially used nowadays. Besides, we have confirmed reliability of FEM analysis, extracted equivalent circuit of transition area, found out factors determining extracted physical values, and made proof of electromagnetic variations for optimum characteristics. In addition, transition structure showing optimized characteristics on the basis of dielectric and microstrip structure was proposed. We reckon that the result of this research will apply with effect to transition design in microwave packaging development.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.13
• /
• pp.5463-5468
• /
• 2014

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.9
• /
• pp.5533-5537
• /
• 2013

MicroRNA-10b (miR-10b) has been reported to play an important role in some types of cancer, but the effects and possible mechanisms of action of miR-10b in the metastasis of nasopharyngeal carcinoma cells (NPC) have not been explored. The aim of the present study was to investigate the function of miR-10b in nasopharyngeal carcinoma and to determine the molecular mechanisms underlying its action. The MTT assay was used to assess proliferation of CNE-2Z cells. Wound healing and transwell migration assays were applied to assess cell migration and invasion, while and expression of E-cadherin and MMP-9 were detected using Western blot analysis. Real-time PCR was employed to detect the expression of genes related to migration and invasion and the $2^{-{\Delta}{\Delta}Ct}$ method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.

• The Journal of Korean Academy of Prosthodontics
• /
• v.43 no.4
• /
• pp.415-425
• /
• 2005

Statement of problem : Fracture of composite resin core will be occulted by progress of crack. Bonding interface of different materials has large possibility of starting point of crack line. Therefore, the bond strength of glass fiber post to composite resin core is important for prevention of fracture. Purpose: This in vitro study tried to find out how to get the higher strength of glass fiber post to composite resin core through surveying the maximum load that fractures the post and cote complex. Materials and methods: 40 specimens made with glass fiber Posts(Style $post^{(R)}$, Metalor, Swiss) and composite resin core ($Z-100^{(R)}$, 3M, USA) were prepared and loaded to failure with push-out type shear-bond strength test in a universal test machine. The maximum fracture load and fracture mode were investigated in the specimens that were restored with four different surface treatments. With the data. ANOVA test was used to validate the significance between the test groups, and Bonferroni method was used to check if there is any significant statistical difference between each test group. Evely analysis was approved with 95% reliance. Results: On measuring the maximum fracture load of specimens, both the treatments of sandblasted and acid-etched one statistically showed the strength increase rather than the control group (p<0.005). The scanning electric microscope revealed that sand blasting made more micro-retention form not only on the resin matrix but on the glass fiber, and acid-etching contributed to increase in surface retention form, eliminated the inorganic particles in resin matrix. Specimen fracture modes investigation represented that sand blasted groups showed lower bonding failure than no-sand blasted groups. Conclusion: Referring to the values of maximum fracture load of specimens, the bonding strength was increased by sand blasting and acid-etching.

• Journal of Welding and Joining
• /
• v.28 no.5
• /
• pp.45-50
• /
• 2010

The fatigue crack growth characteristics of base metal and weld joint of 9% Ni steel for LNG storage tank was carried out using CT specimen at room temperature and $-162^{\circ}C$. Fatigue crack growth rate of base and weld metals at RT and $-162^{\circ}C$ was coincided with a single line independent of the change of stress ratio and temperature. In the region of lower stress intensity factor range, fatigue crack growth rate at $-162^{\circ}C$ was slower than that at RT, and the slop of fatigue crack growth rate at $-162^{\circ}C$ increased sharply with propagating of fatigue crack, fatigue crack growth rate at RT and $-162^{\circ}C$ was intersected near the region of $2{\times}10-4\;mm$/cycle, and after the intersection region, fatigue crack growth rate at $-162^{\circ}C$ was faster than that at RT. The micro-fracture mechanism using SEM shows the ductile striation in the stable crack growth region. Also the defects of weld specimen after fatigue testing were detected using the A scan of ultrasonic apparatus.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1997.04a
• /
• pp.55-62
• /
• 1997

In Japan, the membrane filtration is becoming a common technology for municipal water supply system especially for small plant. 6 years before (1991), the national research project of membrane filtration for small plant has started. The project was named as "MAC 21", MEMBRANE AQUA CENTURY 21. In the project the Ministry of Health and Welfare, 8 universities and 18 water treatment plant companies have been involved. This was the first attempt to research a common theme in joint with government, universities and private companies. After three years, the guide line for membrane filtration application for small plant has been established. This has promoted to install some actual plant. And also, another joint research for "RESEARCH OF MEMBRANE FILTRATION FOR ADVANCED WATER TREATMENT" has started in 1994 and completed in March, 1997. The project was named as MAC21. In the former project the main objectives were removal of turbidity and bacteria from water. However, in new project the objective was establishment of the further advanced membrane filtration technology which would be applicable for trace chemical components removal such as tri-halo-methane pre-courser, agricultural chemicals removal, offensive smell and taste removal and virus removal. For the objectives, application of nanofiltration and hybrid-system, a combination of micro-filtration ultra-filtration with biological, ozone and activated carbon treatment process have been studied. In addition, application of membrane filtration for treatment of back-wash waste water originated from membrane filters and conventional sand filters has.been studied. At the end of March of this year, about 30 membrane filtration plants are actually supplying the water, the total treatment capacity is about 6,000 m$^{3}$/day and another 20 will be installed within one year.led within one year.

• Molecules and Cells
• /
• v.23 no.2
• /
• pp.228-238
• /
• 2007

Since molecular structure of hnRNP is not available in foreseeable future, it is best to construct a working model for hnRNP structure. A geometric problem, assembly of $700{\pm}20$ nucleotides with 48 proteins, is visualized by a frame work in which all the proteins participate in primary binding, followed by secondary, tertiary and quaternary binding with neighboring proteins without additional import. Thus, 40S hnRNP contains crown-like secondary structure (48 stemloops) and appearance of 6 petal (octamers) rose-like architectures. The proteins are wrapped by RNA. Co-transcriptional folding for RNP fibril of FMR1 gene can produce 2,571 stem-loops with frequency of 1 stem-loop/15.3 nucleotides and 53 40S hnRNP beaded structure. By spliceosome driven reactions, there occurs removal of 16 separate lariated RNPs, joining 17 separate beaded exonic structures and anchoring EJC on each exon junction. Skipping exon 12 has 5'GU, 3'AG and very compact folding pattern with frequency of 1 stem-loop per 12 nucleotides in short exon length (63 nucleotides). 5' end of exon 12 contains SS (Splicing Silencer) element of UAGGU. In exons 10, 15 and 17 where both regular and alternative splice sites exist, SS (hnRNP A1 binding site) is observed at the regular splicing site. End products are mature FMR-1 mRNP, 4 species of Pri-microRNAs derived from introns 7,9,15 and 3'UTR of exon17, respectively. There may also be some other regulatory RNAs containing ALU/Line elements as well.

• Journal of the Korean Society of Safety
• /
• v.20 no.1 s.69
• /
• pp.18-23
• /
• 2005

This study deals with the friction and wear characteristics of C-N coated SCM415 steel. The PSII(plasma source ion implantation) apparatus was built and a SCM415 test piece with steel substrate was treated with carbon nitrogen by this apparatus. The composition and structure of the surface layer were analyzed and compared with that of PVD(physical vapor decomposition) coated TiN layer. It was found that both of friction coefficient of C-N coating and TiN coating decreased with increasing load, however, C-N coating showed relatively lower faction coefficient than that of TiN coating. The micro-vickers hardness of C-N film is 3200 Hv, which is $32\~43\%$ higher than that of TiN film. The critical load of C-N film is 52N, which is $25\%$ higher than that of TiN film. The hardness of C-N film fabricated by Plasma ion implantation is $61\~70\%$ higher than that of base material, and faction coefficient is $14\~50\%$ lower than that of base material. It is also interesting to note that the friction was changed from adhesive wear mode to light oxidizing wear mode.