• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.12
• /
• pp.1790-1795
• /
• 2016

MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p, MyoD, MyoG, Myf5, Mef2c, and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.

• Journal of Veterinary Science
• /
• v.24 no.2
• /
• pp.20.1-20.13
• /
• 2023

Background: As Actinobacillus pleuropneumonniae (APP) infection causes considerable losses in the pig industry, there is a growing need to develop effective therapeutic interventions that leverage host immune defense mechanisms to combat these pathogens. Objectives: To demonstrate the role of microRNA (miR)-127 in controlling bacterial infection against APP. Moreover, to investigate a signaling pathway in macrophages that controls the production of anti-microbial peptides. Methods: Firstly, we evaluated the effect of miR-127 on APP-infected pigs by cell count/enzyme-linked immunosorbent assay (ELISA). Then the impact of miR-127 on immune cells was detected. The cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-6 were evaluated by ELISA. The expression of cytokines (anti-microbial peptides [AMPs]) was assessed using quantitative polymerase chain reaction. The expression level of IL-6, TNF-α and p-P65 were analyzed by western blot. The expression of p65 in the immune cells was investigated by immunofluorescence. Results: miR-127 showed a protective effect on APP-infected macrophage. Moreover, the protective effect might depend on its regulation of macrophage bactericidal activity and the generation of IL-22, IL-17 and AMPs by targeting sphingosine-1-phosphate receptor3 (SIPR3), the element involved in the Toll-like receptor (TLR) cascades. Conclusions: Together, we identify that miR-127 is a regulator of S1PR3 and then regulates TLR/nuclear factor-κB signaling in macrophages with anti-bacterial acticity, and it might be a potential target for treating inflammatory diseases caused by APP.

• Journal of Convergence for Information Technology
• /
• v.8 no.3
• /
• pp.127-136
• /
• 2018

This study was to identify factors influencing satisfaction on clinical practice of dental hygienist students located in G, J region. The data was analyzed by t-test, Anova, Pearson correlation coefficient, and multiple regression using SPSS 21.0 program. There were significant in satisfaction of score(F=2.925, p<.05), perceive heath status(F=8.108, p<.001), satisfaction on department(F=8.198, p<.001). The relationship on peers with score(r=.277, p=.01) and perceive health status correlations with relationships on peers(r=.327, p=.01), The satisfaction on clinical practice had positive correlations with score(r=.127, p=.05), perceive health status(r=.226, p=.01), relationship on peers(r=.240, p=.01). The factors influencing satisfaction on clinical practice were satisfaction of relationship on peers, satisfaction of department, perceive health status. The results suggest that development of program to improve satisfaction on clinical practice of dental hygiene students.

• Journal of The Korean Society of Inherited Metabolic disease
• /
• v.15 no.3
• /
• pp.127-137
• /
• 2015

Purpose: The main aim of this study was to compare and analyze expression profiles of microRNAs (miRNAs) to establish miRNA signature of Fabry nephropathy related epithelial mesenchymal transition (EMT). Methods: Expression profiles of miRNAs in kidney tissue samples and cell lines from normal and Fabry disease mouse model were examined by miRNA expression microarray analysis followed by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Results: In the miRNA expression microarray analysis of Fabry mouse kidney tissues compared to wild type mouse, 5 and 3 miRNAs among 1,247 miRNAs examined were up- and down-regulated, respectively. Among them, miR-149-5p was down-regulated about 2-fold in Fabry kidney samples. The down-regulations of miR-149-5p were observed in kidney tissues of under 35 week-old-Fabry mice. However, this down-regulation was not observed in kidney tissues of 42 week-old Fabry mice. In SV40 MES 13 cells, mouse mesangial cells, treated with globotriaosylsphingosine (lyso-Gb3), miR-149-5p was also downregulated. The down-regulation of miR-149-5p induced up-regulation of its target genes related to EMT. Conclusion: The miRNA expression array and qRT-PCR results show that miR-149-5p expression was decreased in kidney tissues of Fabry mice compared to wild type mice under 35 weeks of age. Along with the observation of miR-149-5p expression in Fabry disease cell models, these results indicate that the down-regulated miR-149-5p were related to the biological response of mesangial cells to lyso-Gb3 and also have influence to the transcriptional up-regulation of its target genes. These results suggest miR-149-5p might play important roles in the Fabry nephropathy.

• Journal of Hospice and Palliative Care
• /
• v.13 no.2
• /
• pp.89-97
• /
• 2010

Purpose: This study investigated the relationship between the needs for hospice care and quality of life in cancer patients. Methods: Data were collected from 127 cancer patients hospitalized at a university hospital in Chonbuk between October, 2006 to March, 2007. Two instruments were used: cancer patients' need for hospice care, developed by Kang and Kim and quality of life developed by Tae et al. Results: The mean scores were 3.11 out of 4 for hospice care needs, and 5.25 out of 10 for quality of life. The correlation between needs for hospice care and quality of life was moderate and negative (r=-0.395, P<0.01). Among the four groups of needs, physical need was the most strongly correlated with quality of lifer (r=-0.388, P<0.01). The need for hospice care was significantly different according to participants' religion (t=6.02, P<0.05), and duration of disease (F=3.45, P< 0.05). Quality of life was significantly different according to participants' monthly income (F=3.38, P<0.05). cancer stage (F=8.10, P<0.01) and chemotherapy (t=6.09, P=0.015). Conclusion: The results suggested that the cancer patients' hospice care needs should be answered in order to improve their quality of life. While doing so, participants' characteristic need should also be considered.

• Research in Community and Public Health Nursing
• /
• v.20 no.3
• /
• pp.316-326
• /
• 2009

Purpose: The purpose of this study was to examine teenagers' Internet addiction and to find factors influencing it. Methods: Data were collected from 596 middle and high school students between June 1 and June 20, 2009 with a self-rating questionnaire. The data were processed with SPSS/WIN 12.0. Results: (1) 20.3% of the students were spending over three hours a day in Internet use. The percentages of mild and serious Internet addiction were 22.3 and 2.1, respectively. (2) The stronger Internet addiction was, the higher stress was and the lower social support was. There was a negative correlation between stress and social support (r=-.296, p<.01). There was also a positive correlation between Internet addiction and stress (r=.264, p<.01) while a negative correlation between Internet addiction and social support (r=-.127, p<.01). (3) Among the subjects' characteristics, sex, school and family type, frequency of processed food intake, average daily Internet use hour and sleeping hour, subjective health perception, and satisfaction level with parents and family made difference in the Internet addiction level. Conclusion: The result of this study will provide useful background data for the selection of target population, stress management and social support promotion programs to prevent teenagers' Internet addiction.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.24 no.3
• /
• pp.279-289
• /
• 2018

Purpose: The study is to confirm the influence of self-efficacy, critical thinking disposition, self-leadership, and communication competency on the core competencies of the preceptor. Methods: Data were collected from August 27 to September 7, 2017 using structured questionnaires for 127 preceptors at a general hospital located in Korea. SPSS/Win 23.0 was used to analyze the frequency, percentage, mean, standard deviation, independent t-test, one-way ANOVA, Pearson's correlation coefficients, and stepwise multiple regression. Results: The mean score of the core competencies of the preceptors was $3.94{\pm}0.35$ (range 1-5); self-efficacy was $3.88{\pm}0.49$; critical thinking disposition was $3.54{\pm}0.36$; self-leadership was $3.66{\pm}0.39$; communication competency was $3.71{\pm}0.35$. There were no statistically significant differences between the core competencies of the preceptor and general characteristics. The core competencies of the preceptors were significantly correlated with self-efficacy (r=.61, p<.001), critical thinking disposition (r=.69, p<.001), self-leadership (r=.59, p<.001), and communication competency (r=.58, p<.001). The core competencies of the preceptors were affected by critical thinking disposition (${\beta}=.54$, p<.001), followed by communication competency (${\beta}=.24$, p<.001). The explanatory power by the factors was 51% (F=63.66, p<.001). Conclusion: The results suggest that critical thinking disposition and communication competency are important predictors of the core competencies of the preceptors.

• Korean Journal of Adult Nursing
• /
• v.22 no.1
• /
• pp.60-69
• /
• 2010

Purpose: The aim of this study was to investigate the level of resilience and related factors in patients with Parkinson's disease (PD) in Korea. Methods: Data were obtained from 148 patients using the Resilience Scale (RS), Beck's Depression Inventory (BDI), and Spielberger's Anxiety Inventory (AI). Results: The mean scores of the RS, BDI, and AI were $127.7{\pm}21.6$, $12.9{\pm}9.3$, and $41.9{\pm}11.1$, respectively. The RS score was strongly correlated with the BDI score (r=-.531, p<.001) and the AI (r=-.572, p<.001). The resilience was significantly revealed by household income (F=4.002, p=.009) and presence of a hobby (t=-3.300, p=.001). In addition, resilience was significantly correlated with age of disease onset (r=.164, p=.046), years of living with PD (r=-.262, p=.001), and the length of treatment with levodopa (r=-.283, p<.001). From the stepwise multiple regression analysis, the most important factors related to the RS score were the AI score, household income, and length of treatment with levodopa. Conclusion: Understanding these factors is essential for developing effective interventions to improve resilience in patients with PD.

• Journal of East-West Nursing Research
• /
• v.23 no.1
• /
• pp.18-25
• /
• 2017

Purpose: The purpose of this study was to identify factors which effect to female college student' reproductive health promoting behavior in female college students. Methods: The design of this study was correlational study and subjects were 127 female college students from two colleges in B metropolitan city. The data collection was carried out from June 1, 2016 to June 30, 2016. Data were collected using questionaires of characteristics of the subjects, sexual knowledge, sexual behavior, and self-efficacy(KGSE). Results: The subjects' reproductive health promoting behavior differed according to the grade, major, smoking, and experience of weight loss of more than 10 kg within the last 3 months. The subjects' reproductive health promoting behavior had positive correlations with sexual knowledge (r=.39, p<.001) and self-efficacy (r=.34, p<.001), and negative correlation with sexual behavior (r=-.23, p=.011). Sexual knowledge and sexual behavior were influencing factors on reproductive health promoting behavior accounting for 23.0%. Conclusion: This study showed that sexual knowledge and sexual behavior were factors affecting female college students' reproductive health promoting behavior and the most influential factor was sexual knowledge.

• Molecules and Cells
• /
• v.38 no.10
• /
• pp.895-903
• /
• 2015

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.