• Asian-Australasian Journal of Animal Sciences
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• 제29권12호
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• pp.1790-1795
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• 2016

MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p, MyoD, MyoG, Myf5, Mef2c, and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.

• Journal of Veterinary Science
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• 제24권2호
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• pp.20.1-20.13
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• 2023

Background: As Actinobacillus pleuropneumonniae (APP) infection causes considerable losses in the pig industry, there is a growing need to develop effective therapeutic interventions that leverage host immune defense mechanisms to combat these pathogens. Objectives: To demonstrate the role of microRNA (miR)-127 in controlling bacterial infection against APP. Moreover, to investigate a signaling pathway in macrophages that controls the production of anti-microbial peptides. Methods: Firstly, we evaluated the effect of miR-127 on APP-infected pigs by cell count/enzyme-linked immunosorbent assay (ELISA). Then the impact of miR-127 on immune cells was detected. The cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-6 were evaluated by ELISA. The expression of cytokines (anti-microbial peptides [AMPs]) was assessed using quantitative polymerase chain reaction. The expression level of IL-6, TNF-α and p-P65 were analyzed by western blot. The expression of p65 in the immune cells was investigated by immunofluorescence. Results: miR-127 showed a protective effect on APP-infected macrophage. Moreover, the protective effect might depend on its regulation of macrophage bactericidal activity and the generation of IL-22, IL-17 and AMPs by targeting sphingosine-1-phosphate receptor3 (SIPR3), the element involved in the Toll-like receptor (TLR) cascades. Conclusions: Together, we identify that miR-127 is a regulator of S1PR3 and then regulates TLR/nuclear factor-κB signaling in macrophages with anti-bacterial acticity, and it might be a potential target for treating inflammatory diseases caused by APP.

• 융합정보논문지
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• 제8권3호
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• pp.127-136
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• 2018

본 연구는 G, J 지역의 치위생(학)과 학생들을 대상으로 임상실습만족도에 영향을 미치는 요인을 조사하기 위해 시행되었다. 수집된 자료는 SPSS 21.0 프로그램을 이용하여 t-검정, 일원분산분석을 사용하였고, 상관관계는 피어슨 상관계수로 임상실습만족도에 영향을 미치는 요인은 다중회귀분석을 사용하였다. 대상자의 일반적인 특성에 따른 임상실습만족도의 차이는 건강상태(F=8.108, p<.001), 학과만족도(F=16.372, p<.001), 교우관계(F=8.198, p<.001)에서 통계적으로 유의하게 나타났다. 대상자의 교우관계와 학업성적(r=.277, p=.01), 건강상태와 교우관계에서도 정적상관관계(r=.327, p=.01)를 보였다. 또한 임상실습만족도와 학업성적(r=.127, p=.05), 건강상태(r=.226, p=.01), 교우관계(r=.240, p=.01)에서도 상관관계를 나타내었다. 본 연구 대상자의 임상실습만족도에 영향을 미치는 요인은 교우관계, 학과 만족도, 건강상태로 나타났고, 본 연구결과를 통하여 치위생(학)과 학생들을 대상으로 임상실습만족도를 향상시킬 수 있는 프로그램 개발이 필요하다.

• 대한유전성대사질환학회지
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• 제15권3호
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• pp.127-137
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• 2015

본 연구에서는 파브리병의 마우스 모델과 세포모델을 대상으로 miRNA expression microarray를 적용시켜 질환 모델과 정상 대조군 간의 전체 miRNA의 발현 차이를 조사하였고, 발현량에서 차이를 보인 특정 miRNA를 선별한 후, 해당 miRNA의 표적 유전자의 발현량 변화를 살펴보아 파브리병의 신장병변에 대한 바이오마커 발굴과 발병기전을 알아보고자 하였다. MicroRNA array 결과, 파브리 마우스 신장 조직의 경우, 1,247개의 분석 대상 miRNA 중 5개가 발현이 증가되어 있으며 3개가 발현이 감소되어 있음을 확인하였다. 그 중에서 miR-149-5p의 발현이 파브리 마우스의 신장에서 2배 이상 감소되어 있으며, 특히 35주령 이하의 파브리 마우스에서 이러한 감소현상이 나타남을 확인하였고, 또한 lyso-Gb3를 처리하여 배양한 SV40 MES 13 세포에서도 miR-149-5p의 발현이 감소됨을 알 수 있었다. miR-149-5p의 발현감소는 EMT와 관련된 유전자의 발현을 증가시킴을 확인하였다. 본 연구를 통해 miR-149-5p의 생체지표로서의 가능성과 함께 miR-149-5p의 발현감소가 EMT를 통한 파브리병에서의 사구체 섬유화에 관여할 것이라는 가능성을 제시하고 있다.

• Journal of Hospice and Palliative Care
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• 제13권2호
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• pp.89-97
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• 2010

목적: 본 연구는 호스피스 간호 요구도와 삶의 질을 파악하여 호스피스 간호 중재 방안을 모색하고자 시도 되었다. 방법: 자료수집기간은 2006년 10월부터 2007년 3월까지이며 전라북도 소재 1개 대학 병원에 입원한 암환자 127명을 대상으로 하였다. 연구도구는 Kang과 Kim이 개발한 암환자의 호스피스 간호 요구도 측정도구와 Tae 등이 개발한 삶의 질 측정도구를 이용하였다. 수집된 자료는 SPSS Win 12.0을 이용하였으며, 실수와 백분율, 평균, 표준편차 t-test, ANOVA, Pearson correlation을 이용하여 분석하였다. 결과: 대상자의 호스피스 간호 요구도는 4점 만점에 평점 3.11점이었으며 가족지지 영역이 3.15점으로 가장 높게 나타났으며 다음이 심리적 영역이 3.12점, 신체적 영역이 3.10점, 영적 영역이 3.05점의 순으로 나타났다. 대상자의 삶의 질은 10점 만점에 평점 5.25점이었으며 영적 영역이 5.84점으로 가장 높았고 다음 심리적 영역이 5.18점, 신체적 영역이 5.06점, 사회적 영역이 5.01점의 순이었다. 대상자의 호스피스 간호 요구도와 삶의 질은 호스피스 간호 요구도가 높을수록 삶의 질이 낮게 나타나는 역 상관관계를 보였으며(r=-0.395, P=0.000), 호스피스 간호 요구도 4개 영역 중 신체적 요구가 삶의 질과 가장 강한 역 상관관계를 보였다(r=-0.388, P= 0.000). 대상자의 특성별 호스피스 간호 요구도는 종교(t=6.02, P=0.016), 암 투병기간(F=3.45, P=0.019)에 따라 차이가 있었으며, 삶의 질은 월수입(F=3.38, P=0.021), 암 병기(F=8.10, P=0.000), 항암화학요법(t=6.09, P=0.015)에서 통계적으로 유의한 차이가 있었다. 결론: 결론적으로 암환자의 삶의 질을 향상시키기 위해서는 호스피스 간호 요구를 파악하여야 하며, 동시에 종교, 암 투병기간, 월수입, 암 병기 등과 같은 대상자의 특성을 고려하여야 함을 제안하다.

• 지역사회간호학회지
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• 제20권3호
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• pp.316-326
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• 2009

Purpose: The purpose of this study was to examine teenagers' Internet addiction and to find factors influencing it. Methods: Data were collected from 596 middle and high school students between June 1 and June 20, 2009 with a self-rating questionnaire. The data were processed with SPSS/WIN 12.0. Results: (1) 20.3% of the students were spending over three hours a day in Internet use. The percentages of mild and serious Internet addiction were 22.3 and 2.1, respectively. (2) The stronger Internet addiction was, the higher stress was and the lower social support was. There was a negative correlation between stress and social support (r=-.296, p<.01). There was also a positive correlation between Internet addiction and stress (r=.264, p<.01) while a negative correlation between Internet addiction and social support (r=-.127, p<.01). (3) Among the subjects' characteristics, sex, school and family type, frequency of processed food intake, average daily Internet use hour and sleeping hour, subjective health perception, and satisfaction level with parents and family made difference in the Internet addiction level. Conclusion: The result of this study will provide useful background data for the selection of target population, stress management and social support promotion programs to prevent teenagers' Internet addiction.

• 한국간호교육학회지
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• 제24권3호
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• pp.279-289
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• 2018

Purpose: The study is to confirm the influence of self-efficacy, critical thinking disposition, self-leadership, and communication competency on the core competencies of the preceptor. Methods: Data were collected from August 27 to September 7, 2017 using structured questionnaires for 127 preceptors at a general hospital located in Korea. SPSS/Win 23.0 was used to analyze the frequency, percentage, mean, standard deviation, independent t-test, one-way ANOVA, Pearson's correlation coefficients, and stepwise multiple regression. Results: The mean score of the core competencies of the preceptors was $3.94{\pm}0.35$ (range 1-5); self-efficacy was $3.88{\pm}0.49$; critical thinking disposition was $3.54{\pm}0.36$; self-leadership was $3.66{\pm}0.39$; communication competency was $3.71{\pm}0.35$. There were no statistically significant differences between the core competencies of the preceptor and general characteristics. The core competencies of the preceptors were significantly correlated with self-efficacy (r=.61, p<.001), critical thinking disposition (r=.69, p<.001), self-leadership (r=.59, p<.001), and communication competency (r=.58, p<.001). The core competencies of the preceptors were affected by critical thinking disposition (${\beta}=.54$, p<.001), followed by communication competency (${\beta}=.24$, p<.001). The explanatory power by the factors was 51% (F=63.66, p<.001). Conclusion: The results suggest that critical thinking disposition and communication competency are important predictors of the core competencies of the preceptors.

• 성인간호학회지
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• 제22권1호
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• pp.60-69
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• 2010

Purpose: The aim of this study was to investigate the level of resilience and related factors in patients with Parkinson's disease (PD) in Korea. Methods: Data were obtained from 148 patients using the Resilience Scale (RS), Beck's Depression Inventory (BDI), and Spielberger's Anxiety Inventory (AI). Results: The mean scores of the RS, BDI, and AI were $127.7{\pm}21.6$, $12.9{\pm}9.3$, and $41.9{\pm}11.1$, respectively. The RS score was strongly correlated with the BDI score (r=-.531, p<.001) and the AI (r=-.572, p<.001). The resilience was significantly revealed by household income (F=4.002, p=.009) and presence of a hobby (t=-3.300, p=.001). In addition, resilience was significantly correlated with age of disease onset (r=.164, p=.046), years of living with PD (r=-.262, p=.001), and the length of treatment with levodopa (r=-.283, p<.001). From the stepwise multiple regression analysis, the most important factors related to the RS score were the AI score, household income, and length of treatment with levodopa. Conclusion: Understanding these factors is essential for developing effective interventions to improve resilience in patients with PD.

• 동서간호학연구지
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• 제23권1호
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• pp.18-25
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• 2017

Purpose: The purpose of this study was to identify factors which effect to female college student' reproductive health promoting behavior in female college students. Methods: The design of this study was correlational study and subjects were 127 female college students from two colleges in B metropolitan city. The data collection was carried out from June 1, 2016 to June 30, 2016. Data were collected using questionaires of characteristics of the subjects, sexual knowledge, sexual behavior, and self-efficacy(KGSE). Results: The subjects' reproductive health promoting behavior differed according to the grade, major, smoking, and experience of weight loss of more than 10 kg within the last 3 months. The subjects' reproductive health promoting behavior had positive correlations with sexual knowledge (r=.39, p<.001) and self-efficacy (r=.34, p<.001), and negative correlation with sexual behavior (r=-.23, p=.011). Sexual knowledge and sexual behavior were influencing factors on reproductive health promoting behavior accounting for 23.0%. Conclusion: This study showed that sexual knowledge and sexual behavior were factors affecting female college students' reproductive health promoting behavior and the most influential factor was sexual knowledge.

• Molecules and Cells
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• 제38권10호
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• pp.895-903
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• 2015

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.