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Effect of Heparin on Expression of mRNA of MMP 1,2,9 in Adult Rat Wound (헤파린이 쥐의 창상치유에서 MMP-1,2,9 mRNA의 발현에 미치는 영향)

  • Kim, Han Koo;Hong, Jung Soo;Kim, Sae Hwan;Lee, Mi Kyoung;Kim, Seung Hong;Kim, Woo Seob
    • Archives of Plastic Surgery
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    • v.34 no.2
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    • pp.149-155
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    • 2007
  • Purpose: This study was to investigate how the heparin, which has been known to induce neovascularization by MMP in the infarcted tissue of the myocardium, had influence on the expression of mRNA of MMP 1,2,9 of the skin wound of rat. Methods: Full depth skin wounds were created on the dorsum of Sprague-Dawley 60 rats. The experimental rats were divided into two groups according to the concentration of heparin($100{\mu}g/ml$ in 20, $300{\mu}g/ml$ in 20). Heparin soaked gelatin sponges in different concentration were inserted into the pocket of experimental rats and the wounds were closed. Normal saline soaked gelatin sponges were used in control rats. Wounds were harvested at 48 and 72 hours after closure. We performed histologic study in H-E stain. RNA was isolated from the harvested tissue and then real time polymerase chain reaction was performed to determine the gene expression of MMP-1,2,9. Results: We observed that inflammatory cell decreased in heparin soaked group and heparin increased the expression of MMP-1,9 mRNA of dorsal wound of rat at 72 hours (p < 0.05). Conclusion: This result suggest that heparin may be used inducing another factor inducing scarless wound healing by increasing MMP.

A Study on the Relationship Between Perceived Family Support & Self Concept of Cesarean Section Mothers (가족의 지지와 제왕절개술 산모의 자아개념간의 관계연구)

  • Chang, Myung-Ja;Lee, Mi-La
    • 모자간호학회지
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    • v.1
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    • pp.23-33
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    • 1991
  • Recently, Cesarean Section has been revealed as major events in maternity nursing, because it has influence on tile formatter of the self concept of cesarean section mothers as well as later coping mechanism and mother-child relationship including the mothering role. The study is conducted to clarify the relationships between the perceired family support and the self concept of cesarean section mothers, and to find out other Influencing factors on the formation of the self concept. Measuring instrument used in this study was Lee's scale to measure the self concept and the degree of perceived family support of the cesarean section mothers, The subject were 155 cesarean section mothers who were selected conveniently. Volunteers answered self reporting questionnaires which asked about their self concept. Data was analyzed as follows using SAS. The relationship between scores of the self concept and degree of the perceived family support was analyzed by Pearson's product moment correlation. In order to identify the influencing effects of demographic variables on the formation of self concept & F test were used. Result were summarized as follows : 1. Scores of self concept of cesarean section mothers were significantly correlated with scores of the perceived family support (r=0.3438, p<0.01) These results show that the family support is closely related to the self concept of cesarean mothers and has positive effects on their coping capacity. 2. Scores of self-concept of those who have lower ducation background and are multipara were significantly higher than those who have higher education background and are primipara(M=29.90$\pm$9.37, 27.69$\pm$3.89, t=2.006, df=105.8, p=0.0480: M=27.75$\pm$3.49. 29.46$\pm$8.68, t=-1.7183, 2. p=0.08).

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A Structural Equation Model of Clinical Nurses' End-of-life Care Performance (임상간호사의 임종간호수행 구조모형)

  • Park, Hyo jin;Lee, Yun Mi;Kim, Min Hye
    • Journal of Korean Critical Care Nursing
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    • v.14 no.1
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    • pp.1-13
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    • 2021
  • Purpose : Based on Quint's theory and the relevant literature, this study constructed a structural equation model for explaining and predicting end-of-life care performance in clinical nurses. Methods : A self-administered questionnaire was used to collect data from 265 nurses between September 1 and September 30, 2016. The data were analyzed using SPSS ver. 21 and AMOS ver. 21. Results : The goodness of fit of the modified model was found to be relatively satisfactory (χ2=114.82, Nomed χ2(χ2/df)=2.44, SRMR=.06, GFI=.94, AGFI=.89, CFI=.95, TLI=.91, RMSEA=.07). End-of-life care performance was affected by the attitudes toward nursing care of the dying, working unit, and death anxiety. The attitudes toward such care had the highest effect on end-of-life care performance. Conclusion : The results suggest that end-of-life care performance is directly and indirectly affected by attitudes toward nursing care of the dying, participation in end-of-life care education, working unit, death perception, and death anxiety. To improve clinical nurses' end-of-life care performance, effective programs to promote death anxiety and attitudes toward nursing care of the dying need to be developed. In addition, hospital nursing organizations should attempt to produce concrete measures for death anxiety and terminal care attitudes in clinical nurses.

Pathway Analysis on the Effects of Nursing Informatics Competency, Nursing Care Left Undone, and Nurse Reported Quality of Care on Nursing Productivity among Clinical Nurses (간호정보역량, 미완료간호, 환자간호의 질이 간호생산성에 미치는 영향에 관한 경로분석)

  • Yu, Mi;Kim, Se Young;Ryu, Ji Min
    • Journal of Korean Academy of Nursing
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    • v.53 no.2
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    • pp.236-248
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    • 2023
  • Purpose: Nursing informatics competency is used to manage and improve the delivery of safe, high-quality, and efficient healthcare services in accordance with best practices and professional and regulatory standards. This study examined the relationship between nursing informatics competency (NIC), nursing care left undone, and nurse reported quality of care (NQoC) and nursing productivity. A path model for their effects on nursing productivity among clinical nurses was also established. Methods: Data were collected using structured questionnaires answered by 192 nurses working in a tertiary hospital located in J city, Korea, and analyzed using SPSS/WIN 23.0 and AMOS 21.0 program. Results: The fit indices of the alternative path model satisfied recommended levels χ2 = .11 (p = .741), normed χ22 /df) = .11, SRMR = .01, RMSEA = .00, GFI = 1.00, NFI = 1.00, AIC = 18.11. Among the variables, NIC (β = .44, p < .001), NQoC (β = .35, p < .001) had a direct effect on nursing productivity. Due to the mediating effect of NQoC on the relationship between NIC and nursing productivity, the effect size was .14 (95% CI .08~.24). Meanwhile, nursing care left undone through NQoC in the relationship between NIC and nursing productivity, has a significant mediation effect (estimate .01, 95% CI .00~.03). The explanatory power of variables was 44.0%. Conclusion: Education and training for enhancing NIC should be provided to improve nursing productivity, quality of care and to reduce missed nursing care. Furthermore, monitoring the quality of nursing care and using it as a productivity index is essential.

Assessment of Regulatory Effect of Electro-acupuncture at Jogsamni($ST_{36}$), on Electrogastrography in Diabetic Patients with Gastric Dysrhythmia;A Case Series Study (위장 비율동성 당뇨환자에서 족삼리(足三里)($ST_{36}$) 전침처치의 위전도 조절 영향 사례군 연구)

  • Oh, Dal-Seok;Choi, Jun-Yong;Jung, So-Young;Shin, Mi-Suk;Kim, Ae-Ran;Kang, Kyung-Won;Kim, Jong-In;Choi, Sun-Mi
    • Journal of Acupuncture Research
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    • v.25 no.4
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    • pp.95-103
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    • 2008
  • Objectives : This study was to investigate the regulatory effect of electro-acupuncture on Jogsamni ($ST_{36}$) in bradygastric condition or gastroparesis which is a neuropathic complication of type 2 diabetes mellitus(T2DM), assessed using 4-channel electrogastrography (EGG). Methods : It was a case series study. Each patient was given electro-acupuncture(EA) one point, Jogsamni($ST_{36}$) or Gyeonu($LI_{15}$) bilaterally for thirty minutes with 2Hz bipolar square wave frequency and moderate intensity of stimulation. With attaching electrodes around stomach region throughout pre-acupuncture, acupuncture, and post-acupuncture session, these parameters were produced; percent rate of bradygastria power compared to sum of power in all kind of gastric rhythm(% bradygastria), dominant frequency(DF) and dominant power(DP). Parameter was analysed per each channel's variable change from pre-acupuncture session by paired t-test. Results : There was statistically significant decrease in % bradygastria parameters on one of the $ST_{36}$ treated patients from pre-acupuncture session and to post-acupuncture session(P=0.015, P=0.008 respectively). A marginal significant decrease of DP was shown in the other $ST_{36}$ treated patient from pre-acupuncture session through post-acupuncture session(P=0.049). Combined data of two $ST_{36}$ Treated patients showed that there was significant decrease of %bradygastria from pre-acupuncture session to acupuncture session(P=0.020), and was decrease of DP from pre-acupuncture session to post- acupuncture session(P=0.020). Conclusions : EA at $ST_{36}$ resulted in statistically significant decrease in %bradygastria and DP for dysrhythmic condition in T2DM gastroparesis patients. Considering this limited results, further study will be needed to elucidate the effect of multiple-acupuncture on gastric myoelectrical activities.

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Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)

  • Yoo, Sung-Yung;Lee, Yong-Ho;Park, So-Hyun;Choi, Kyong-Mi;Park, June-Young;Kim, A-Ram;Hwang, Su-Min;Lee, Min-Ju;Ko, Tae-Seok;Kim, Tae-Wan
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.6
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    • pp.659-664
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    • 2013
  • The aim of this study is to determine the drought stress index through photochemical analysis in red pepper (Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation between Kautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, and $CO_2$ assimilation in drought stressed 5-week-old red pepper plants. The $CO_2$ assimilation rate was severely lowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), in less than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIP process). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22, meaning not only the initial inhibition of PSII but also sequential inhibition of PSI. In real, most of all photochemical parameters such as quantum yield of the electron transport flux from Quinone A ($Q_A$) to Quinone B ($Q_B$), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yield of the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reaction center, and electron transport flux until PSI acceptors per cross section were profoundly reduced except number of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated that at least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC) could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, driving forces (DF) for photochemical activity could be deduced from the performance index (PI) for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion, photochemical responses and their related parameters can be used as physiological DFI.

A epidemiological study on the oral health in preschool children for the development of community based oral health program in Sungnam city (성남시 보건소 유아구강보건사업 개발을 위한 구강건강실태에 관한 조사연구)

  • Ahn, Yong-Soon;Kim, Mi-Jeong
    • Journal of Korean society of Dental Hygiene
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    • v.1 no.2
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    • pp.201-211
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    • 2001
  • Dental caries causes the majority of tooth loss among Koreans. Korea is experiencing an increase in dental caries. And it shows a high prevalence of caries in preschool children, which contrasted with the much-improved situation in developed countries. So, it is important to control caries from preschool periods in Korea. Recently, inspections of the teeth of preschool children for evidence of dental disease became a legislative duty in Korea following Oral Health Act(2000). But, standardized oral health programs in preschool children is not yet proposed. Therefore, it is necessary to develop an community based oral health program in preschool children. The purpose of this study was to obtain basic data for the development of community based oral health program in preschool children of Sungnam city. Oral health status in primary dentition were surveyed in 862 2-6 year-old preschool children of Sungnam city by WHO criteria. From the data, df rate, percentage of preschool child ren with active caries, dft index, dt rate, percentage of preschool children with pit and fissure sealant of each age were calculated, and discussed. The obtained results were as follows : 1. The experience of dental caries in primary dentition showed a tendency to increase quickly between 2 and 3 year old children. So, the oral health program in preschool children should be developed to prevent dental caries and control their oral health under three years of age. 2. In 5 year-old children, the percentage with active caries was 60.8% and dft index was 5.06. The dt rate showed a tendency to decrease as proportion to age, but even though 6 year-old children, the dt rate was 47.7%. Therefore, the annual screening dental examination and oral health education programs at the institution for preschool children should be developed to detect and treat dental caries in primary dentition at early stage. 3. The percentage of preschool children with pit and fissure sealant at six years of age was 10.2%. So, pit and fissure sealant and professional fluoride application programs in Public Health Center should be developed to prevent dental caries. 4. Also, it is necessary to establish oral health goals in preschool children and develop water fluoridation program 10 improve and preserve oral health of preschool children in Seongnam city, effectively.

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Development of Rapid Analysis Method for Pesticide Residues by GC-MS/MS (GC-MS/MS를 이용한 잔류농약 신속검사법 개발)

  • Choi, Yong-Hoon;Nam, Hye-Seon;Hong, Hye-Mi;Lee, Jin-Ha;Chae, Kab-Ryong;Lee, Jong-Ok;Kim, Hee-Yun;Yoon, Sang-Hyeon
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.292-302
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    • 2005
  • Condition of Ion-Trap gas chromatography-mass spectrometry (GC-MS) for rapid screening of 206-pesticides residues in agricultural foodstuffs was optimized. As applying a wide-bore column (10 m${\times}$0.53 mm, DF 0.25 um) connected with a fused silica restrictor (0.6 m${\times}$0.1 mm), a significant retention time reduction was obtained. Additionally, the shape of peaks was sharper and higher than classical GC's and GC-MS's, which allowed lower detection limits. To easily manage many spectral data, both of Electron Ionization(EI) and Chemical Ionization(CI) techniques were adopted in screening procedure. At the following steps, MS-MS technique were used to confirm screened analytes in complicated matrices.