• Journal of Applied Biological Chemistry
• /
• v.58 no.1
• /
• pp.31-38
• /
• 2015

The rhizomes of Anemarrhena asphodeloide was extracted with 100% ethanol and concentrated subfractions were separated with medium pressure liquid chromatography-based activity profiling. One compound was isolated from the subfraction 10 through the repeated preparative high performance liquid chromatography (prep-HPLC). According to physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compound was determined as nyasol (1). Nyasol was exhibited potent inhibitory activity for NO ($IC_{50}:12.5{\mu}g/mL$), tyrosinase ($IC_{50}:12.5{\mu}g/mL$), melanin contents ($IC_{50}:12.5{\mu}g/mL$), thymus and activation-regulated chemokine (TARC) production ($IC_{50}:6.25{\mu}g/mL$). As a result, nyasol has an excellent inflammation-dependent anti-whitening and TARC production activity. It could be used to a large range of functional cosmetics.

• Journal of Life Science
• /
• v.9 no.2
• /
• pp.169-175
• /
• 1999

Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid stresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival mechanism is aquired by the Acid Tolerance Response(ATR) in Salmonella. The ATR of S. typhimurium is a complex inducible phenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more severe acid challenges. ATR in Salmonella has two different systems that are called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$AP had ATR as S. typhimurium UK1. Using the P22 MudJ(Km, lacZ) operon fusion technique and a lethal selection procedure combining low pH(pH4.5) and sodium acetate(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in anaerobic condition(5% $CO_2$, 5% H$_2$, 90% $N_2$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.

• Journal of radiological science and technology
• /
• v.35 no.3
• /
• pp.219-226
• /
• 2012

Interventional radiologists are not aware of the potential injury from procedures. The purpose of this study are to evaluate radiation exposure of interventional radiologist from intervention procedures and to develop guideline of the simple methods for decreasing their radiation exposure from intervention procedure. In this study, Dosimeters were used to monitor operator doses of radiation exposure in a broad range of procedures from 20 interventional radiologist during the periods of 3 months. And, we searched protection methods of each interventional radiologist. During TACE procedure, there was using 0.5 mmPb radiation protector decreased average 89.5 % of radiation exposure. Thicker radiation protector provide decreasing radiation exposure. And radiation exposure dose decreased average 47.7 % by using pulse fluoroscopic mode. Therefore, interventional radiologist should wear protective aprons, use active shielding, monitor their doses, and know how to poisoning themselves during the procedure and operate correct of the machines for minimum dose.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.27 no.4
• /
• pp.465-470
• /
• 2013

The purpose of this study is to observe clinically the effects of red onion prescription on type 2 diabetes. The subject of this study were middle-aged people. We measured clinically physiological index, body composition, radial arterial pulse wave before taking medicine and after taking medicine for 4 month (the prescription of red onion). The study results were as follows. In physiological Index, diastolic blood pressure(mmHg)(p<0.001), Fasting blood glucose(mg/dL)(p=0.001), and HbA1c(%)(p<0.001) were decreased significantly after taking medicine. In body Composition, waist-hip ratio(p=0.031), body fat percentage(p=0.006), fat mass(kg)(p=0.005), and muscle mass(kg)(p=0.001) were decreased significantly after taking medicine. Blood Circulation Index, ECO(L/min)(p=0.038) and ECI($L/min/m^2$)(p=0.007) were increased significantly after taking medicine, ECR($dyne^*sec^*cm^{-5}$)(p=0.002), and ECRI($dyne^*sec/cm$)(p=0.001) were decreased significantly after taking medicine for 4 month. The prescription of red onion was showed significant change in physiological index, character of body composition, blood circulation index. Based on this result, it is considered that red onion prescription has the positive effects on type 2 diabetes and blood circulation clinically. In the future, diversified studies on the effects of red onion prescription are needed.

• Journal of Internet Computing and Services
• /
• v.10 no.1
• /
• pp.145-158
• /
• 2009

No other change in Kora has offered gredter chalenges and opportunities than the emergence of the Internet and its related Information Technology. In the technology for accessing the ever growing wave of information. The quality of the silver generation is increasingly difficult to achieve because of the complexities of life in today's ICT(Information & Comunication Technology) dependent society, Life is more interesting when one knows what is going on, what opportunities exist, and when alternatives to current practices can be discovered and utilized with ease. The purpose of this study is to identify the determinants of attitude and planned behavior toward using internent for the quality of life. This study employ the intention to use internet, perceived ease of use, perceived usefuiness, self-efficacy, stress factor, generation gap, and income as major research variables, and collected 288 survey responses from senior citizen over 55. The results indicate that the perceived usefuiness and ease of use influence the intention to use internet, and that the self-efficacy, the ease of use and generation gap has some impact on the attitude, but the stree factor and the income level do not appear significant to the attitude. It was also found that the self-efficacy and the income level determine the level of ease of use. In addition, some useful suggestions concerning the continuing education program for silver generations are presented.

• Journal of radiological science and technology
• /
• v.32 no.1
• /
• pp.79-86
• /
• 2009

The study found out developmental factors of the liver diseases in 29, 531 cases of the healthy adults who were diagnosed by using ultrasound at domestic healthcare centers in 6 cities. The results are as follows. Based on the result of the study, the liver diseases diagnosed by using ultrasound was revealed to show 43.1% of prevalence, and the occurrence was significantly higher in male (23.3%) than in female (19.8%). The prevalence of hepatic diseases related to the BMI was revealed to show highest prevalence of the fatty liver in obese group (BMI $\geqq$ 25) by recording 44.3%. Smoking contributed to the high prevalence of all liver diseases. Although the fatty liver was the most frequently occurred form of liver diseases by recording the prevalence of 49.1% (22.2% in male, 26.9% in female), the significant difference was found only in female (p < 0.05), but male group did not show significant difference (p > 0.05). The prevalence of hepatic diseases related to the hypertension was revealed to show highest prevalence of the fatty liver in hypertension group by recording 67.7%. The prevalence of hepatic diseases related to the diabetes was revealed to show highest prevalence of the fatty liver in diabetes group by recording 66.2%. The high prevalence of all hepatic diseases was related to diabetes mellitus with statistical significance (p < 0.001). The multiple regression analysis for the related factors which affect the prevalence of the liver diseases showed the higher prevalence by age. Sex, obesity and diabetes mellitus were positively related to the prevalence (p < 0.05) while hypertension and smoking showed no significant relationship to the prevalence of the disease (p > 0.05).

• Journal of Digital Convergence
• /
• v.16 no.3
• /
• pp.201-210
• /
• 2018

WorldJob+, being operated by The Human Resources Development Service of Korea, provides a recruitment support services to overseas companies wanting to hire talented Korean applicants and interns, and support the entire course from overseas advancement information check to enrollment, interview, and learning for young job-seekers. More than 300,000 young people have registered in WorldJob+, an overseas united information network, for job placement. To innovate WorldJob+'s services for young job-seekers, Splunk, a powerful platform for analyzing machine data, was introduced to collate and view system log files collected from its website. Leveraging Splunk's built-in data visualization and analytical features, WorldJob+ has built custom tools to gain insight into the operation of the recruitment supporting service system and to increase its integrity. Use cases include descriptive and predictive analytics for matching up services to allow employers and job seekers to be matched based on their respective needs and profiles, and connect jobseekers with the best recruiters and employers on the market, helping job seekers secure the best jobs fast. This paper will cover the numerous ways WorldJob+ has leveraged Splunk to improve its recruitment supporting services.

• The Journal of Korean Association of Computer Education
• /
• v.8 no.3
• /
• pp.43-60
• /
• 2005

Teaching computer and ICT skills in K-12 helps to bridge the digital divide of the students at the minimum cost, and provides them with a chance of getting a Quality of life as well as a Quality of work in the future. From examining the education policies of U.S., EU, Canada, and other countries, concerning computer education, several factors, including vision and national-level policy, Quality of manpower, needs for computer-related technology, structure of IT industry, overall demand for IT specialists, have been identified as those with major impacts on the curriculum of computer education. Based upon the analysis of domestic key factors, three levels of computer education program are suggested. At the most basic and least controversial level, basic ICT-skill program for effective learning needs to be provided for every student. Next level is concerned with improving the Quality of life and work. A program for introductory level of computer science can be provided as extra-curricular classes for bright and eager students, who might want to be computer expert or specialists.

• Clinical and Experimental Reproductive Medicine
• /
• v.28 no.1
• /
• pp.41-46
• /
• 2001

Objective: To determine the effects of leukemia inhibitory factor (LIF) on embryonal development in in vitro culture. Methods: This is designed in vitro model using eggs from mouse. The eggs from mouse were assigned 29 for control group, 53 for 20 ng/ml of LIF, 88 for 40 ng/ml of LIF, 68 for 80 ng/ml of LIF respectively for in vitro fertilization. And 26 fertilized eggs at 2 cell stage from mouse also were assigned. The mouse embryos of all groups were cultured in medium supplemented with LIF in different concentrations, whereas the eggs in control group was cultured in medium without supplement of LIF. Results: At 72 hours culture of eggs from in vitro fertilization, there was a slight increas in rate of embryonal development to morula in both LIF-20 and LIF-40 as results of 64.15% and 75% respectively, while 42.65% in inferior rate of LIF-80, compare with 51.72% in control group. But the difference between these each groups were not significant in statistically ($p{\le}0.05$). And after 96 hours culture of eggs, the rates blastocyst formation was significantly higher in both LIF-20 and LIF-40 as 56.6% and 63.63% than those in control and LIF-80 as 44.83% and 35.29% respectively. On culturing eggs from in vivo fertilization, the rates of blastocyst formation was significantly not only higher as 85% and 81.81% respectively in medium supplemented with LIF-40 and LIF-80 than 42.3% in LIF-20 but also embryonal cell viability were remakedly improved at 96 hours after culture. Conclusion: The LIF in low dose is embryotrophic, but LIF in high dose is embryotoxic on eggs from in vitro fertilization. Whereas on culturing eggs from in vivo fertilization, LIF is more beneficial with dose dependent in high concentration.

• Korean Journal of Microbiology
• /
• v.50 no.1
• /
• pp.33-39
• /
• 2014

One hundred forty four bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejeon area. Among them, one bacterial strain was selected for this study based on its emulsification activity, growth rate and surface tension activity, and this selected bacterial strain was identified as Pseudomonas sp. HN37 through physiological- biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. HN37 utilize the several aliphatic hydrocarbons, 3,5-dinitrosalicylic acid and 2,4-dichlorophooxyacetic acid as a sole carbon source. And this bacterial strain showed a high resistance to antibiotics such as ampicillin and chloramphenicol, as well as heavy metals such as Ba, Cr, Li and Mn. Also, it was found that the optimal pH and temperature for the cell growth, surface tension, and emulsification activity of Pseudomonas sp. HN37 were pH 6.0-9.0 and $30^{\circ}C$, respectively. The emulsification and surface tension activity was reached the maximum to 1% (V/V) crude oil and 1% (W/V) NaCl concentration. The surface tension of the culture broth was decreased from 62 to 27 dyne/cm after fifteen hours of inoculation in LB media.