• Microbiology and Biotechnology Letters
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• v.19 no.1
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• pp.57-63
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• 1991

A strain J-19 was isolated from soil, produced lipase which has resistant against alkali and linear alkylbenzene sulfonate. The strain was identified as Pseudornonns sp.. The enzyme was purified by ammonium sulfate precipitation, DEAE-Sephadex and Sephadex G- 100 column chromatography. The specific activity of the purified enzyme was 35 unit/mg protein and the yield of enzyme activity was 17%. The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis. Mo1ecul;tr weight of the purified enzyme was estimated about 36,000 by Sephadex GI00 gel filtration and SDS-polyacrylarnide gel electrophoresis. The optimum pH and temperature were pH 10.0 and $30^{\circ}C$, respectively. Activity of the purified enzyme was increased 2-fold by the addition of 0.1% linear alkylbenzene sulfonate and 2.5- fold by the addition of 0.05% Tide. This enzyme remained stable from pH 8.0 to 10.0 and stable up to $40^{\circ}C$.

• Journal of Environmental Science International
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• v.20 no.12
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• pp.1521-1531
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• 2011

This study was carried out to determine the characteristics of the marine environment, including nutritional content, in order to clearly understand the outbreaks of Gonyaulax polygramma in the southern coastal areas in August, 2009. Samples were collected at 13 sites and water temperature and salinity were measured using a CTD. Field surveys were twice: the first between August 5-7, the second between August 22-24. The cell density of G. polygramma was 6,500-10,000 cells $ml^{-1}$ during the $1^{st}$ survey, whereas during the $2^{nd}$ survey the range of the cells was recorded from 8,000 to 12,500 cells $ml^{-1}$. Cochlodinium polykrikoides ranged from 0 to 105 cells $l^{-1}$ during the field survey. In water environments, the majority stations during the $1^{st}$ survey showed a nearly homogeneous water column below $1^{\circ}C$ in temperature, as well as similar profiles of salinity. However, the stratification between the surface and bottom was observed in the $2^{nd}$ survey. Regarding nutrients, Dissolved Inorganic Nitrogen (DIN) in the surface ranged from 0.144 to 0.236 mg $l^{-1}$ during the $1^{st}$ survey, and 0.082-0.228 mg $l^{-1}$ during the $2^{nd}$ survey. DIP (Dissolved Inorganic Phosphorus) did not show any difference in concentration between the $1^{st}$ and $2^{nd}$ survey. During August of 2009, the wind speed in the southern waters remained at around ${\leq}2\;m\;s^{-1}$ for about 60% of time, and there was very little precipitation during the month. Irradiance of ${\geq}10$ hr was shown in the late of August. It is thought that a low level of DIN and salinity play an important role as an essential factor for rapid growth, wide distribution and longer duration of red tide in G. polygramma.

• Journal of the Korean Society of Industry Convergence
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• v.5 no.3
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• pp.213-218
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• 2002

Total nitrogen is a major pollutant which mostly causes eutrophication and red tide. Wastewater effluent from printing of cotton-viscose rayon containing high concentrations of total nitrogen can not be effectively treated with a typical biological treatment process. This paper provides a new treatment process and experimental results for the removal of high strength nitrogen from dyeing wastewater. The optimum conditions of air stripping for the removal of total nitrogen include around pH 12, temperature $60^{\circ}C$ with 60 minutes of stripping time. In case of a filtration-air stripping process, an initial level ($500mg/{\ell}$) of total nitrogen was significantly reduced to below $60mg/{\ell}$. Deconite was synthesised for further decomposition of organic nitrogen. Thus, a filtration-decomposition-air stripping process was possibly achieved, by which a high level ($900mg/{\ell}$) of total nitrogen was effectively removed to below $60mg/{\ell}$ P. Finally, a continuous new process for the removal of total nitrogen is proposed and confirmed, based on batch experimental results, and its process validity is further discussed throughout.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.1
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• pp.451-455
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• 2012

Physicochemical water conditions and phytoplankton were investigated at Ggot-jee beach in Anmyondo, summer season of 2011. COD were sometimes recorded higher than 5.0mg/l and SS were always over 40mg/l. DO, pH and salinity were stable generally but low salinity was affected by heavy rainfall. In spite of relatively high concentrations of chlorophyll and phytoplankton standing stocks, discolored water phenomena(green or red tide) had not been occurred. A few dinoflagellate species were dominated in June and July, but general diatoms were dominant species of phytoplankton community in August. Escherichia coli concentrations were higher in August(>400CFU/100ml) than in July but it's not the level of hygienic problem.

• The Korean Journal of Ecology
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• v.20 no.1
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• pp.61-68
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• 1997

Due to its lack of wave action and tide ecosystem in Inchon dock lacks in marine characteristics. Structural condition in the dock is artificially similar to that of lake. The purposes of this study was to clarify the water quality, to provide the basic physicochemical data and tl resolve the causation of ？미 blooming. Samples were obtained monthly from four stations in Inchon dock during January to December, 1991. Water temperature ranged from $2.7^{\circ}C$ to $27.6^{\circ}C$ under the strong influence of air temperature. Salinity varied between 24.7%-30.4% thus being influenced by freshwater discharged from a spring. Dissolved oxygen was concentrated from 0.1-13.92 mg/l and suspended solids were 6.9-231.0 mg/l. The physicochemical factors were similar to those investigated 10 years ago. However, increased concentration of nitrogenous nutrients initiated ？미 blooming and its process was accelerated to reach eutrophication. Algal blooming was proceeded in March and August.

• Economic and Environmental Geology
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• v.48 no.2
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• pp.147-160
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• 2015

In 2014, on 31 August and 1 September, the emissions of $CH_4$, $CO_2$, and $O_2$ gases were measured six times using the closed chamber method from exposed tidal flat sediments in the same position relative to the low point of the tidal cycle in the Eoeun-ri, Taean-gun, on the Mid-western Coast of Korea. The concentrations of $CH_4$ in the air sample collected in the chamber were measured using gas chromatography with an EG analyzer, model GS-23, within 6 hours of collection, and the other gases were measured in real time using a multi-gas monitor. The gas emission fluxes (source (+), and sink (-)) were calculated from a simple linear regression analysis of the changes in the concentrations over time. In order to see the surrounding parameters (water content, temperature, total organic carbon, average mean size of sediments, and the temperature of the inner chamber) were measured at the study site. On the first day, across three measurements during 5 hours 20 minutes, the observed $CO_2$ flux absorption was -137.00 to $-81.73mg/m^2/hr$, and the $O_2$ absorption, measured simultaneously, was -0.03 to $0.00mg/m^2/hr$. On the second day using an identical number of measurements, the $CO_2$ absorption was -20.43 to $-2.11mg/m^2/hr$, and the $O_2$ absorption -0.18 to $-0.14mg/m^2/hr$. The $CH_4$ absorption before low tide was $-0.02mg/m^2/hr$ (first day, Pearson correlation coefficient using the SPSS statistical analysis is -0.555(n=5, p=0.332, pronounced negative linear relationship)), and $-0.15mg/m^2/hr$ (second day, -0.915(n=5, p=0.030, strong negative linear relationship)) on both measurement days. The emitted flux after low tide on both measurement days reached a minimum of $+0.00mg/m^2/hr$ (+0.713(n=5, p=0.176, linear relationship which can be almost ignored)), and a maximum of $+0.03mg/m^2/hr$ (+0.194(n=5, p=0.754, weak positive linear relationship)) after low tide. However, the absolute values of the $CH_4$ fluxes were analyzed at different times. These results suggest that rate for $CH_4$ fluxes, even the same time and area, were influenced by changes in the tidal cycle characteristics of surface sediments for understanding their correlation with these gas emissions, and surrounding parameters such as physiochemical sediments conditions.

• Korean Journal of Ecology and Environment
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• v.49 no.4
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• pp.320-333
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• 2016

This study was carried out to elucidate the development of unprecedented water-bloom caused by a single species of colonial green algae Eudorina elegans in the upstream area of the Seungchon weir located in the Yeongsan River from late April to May 2013. The Yeongsan River is typically regulated system and the waterbody is seriously enriched by both external and internal sources of nutrients. Seasonal algal outbreaks were highly probable due to various potential factors, such as the excessive nutrients contained in treated wastewater, slow current, high irradiation and temperature, in diatom (winter), green algae (spring) and bluegreen algae (summer). Spring green-tide was attributed to E. elegans with level up to $1,000mg\;m^{-3}$(>$50{\times}10^4cells\;mL^{-1}$). The bloom was exploded in the initial period of the algal development and after then gradually diminished with transporting to the downstream by the intermittent rainfall, resulting in rapid expansion of the distribution range. Although the pulsed-flows by the weir manipulation was applied to control algal bloom, they were not the countermeasures to solve the underlying problem, but rather there still was a remaining problem related to the impact of pulsed-flows on the downstream. The green-tide of E. elegans in this particular region of the Yeongsan River revealed the blooming characteristics of a colonial motile microalga, and fate of vanishing away by the succeeding episodic events of mesoscale rainfall. We believe that the results of the present study contribute to limno-ecological understanding of the green-tide caused by blue-green algae in the four major rivers, Korea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.233-240
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• 1989

The concentration of mecury was measured in the seawater, sediment, Mytilus edulis and suspended solid in the coastal area of Onsan. Samples were collected at spring and neap tide in the period from October 9 to November 2, 1984. The range and mean of mecury concentration in the seawater are $0.08\~0.73{\mu}g/\iota\;and\;0.19{\mu}g/\iota$. The range and mean of mecury concentration in the suspended solid was $0.70\~17.0{\mu}g/g$ TSS and $4.6{\mu}g/g$ TSS respectively. The average concentration of mecury in Mytilus edulis was 0.10mg/kg which was higher than that of the reference area, Chung Mu. The mean value of concentration factor of mercury in wet base is 500 in Mytilus edulis. The average concentration of mecury in sediment was 0.15mg/kg.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.335-335
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• 2015

행주대교 하류 약 3km 지점에 위치한 신곡수중보는 한강종합개발 사업 중 하도정비로 인한 상시수위 저하로 인해 발생하는 문제를 해결하기 위해 설치되었으며, 취수장의 취수심 확보, 주운수심 확보, 염수 역류에 의한 생태계의 변화 방지, 하천 주면의 지하수위 저하 방지 목적으로 건설되었다. 본 연구에서는 신곡수중보의 존치, 이전 혹은 철거로 인한 수질변화를 모의를 실시하였다. 한강 본류와 임진강 합류를 고려하여 총 495개 수질모의 셀을 구성하였고, 수질모의를 위한 WQ Cell은 길이가 100 m~340 m로 임진강 47개, 한강 448개로 총 495개의 셀로 구성하였으며, 주요 지천이나 오염원으로 신곡보 하류에는 공릉천, 신곡보와 잠실보 사이에는 창릉천, 서남과 난지물 재생센터, 안양천, 홍제천, 중랑천, 탄천, 잠실보 상류에는 왕숙천, 월문천, 덕풍천 등 9개 지천과 2개 물 재생센터를 포함하였으며 잠실보 상류에서의 생활용수 취수를 고려하였다. 수온, BOD, 조류의 경계조건은 환경부에서 제공한 2011년 3월과 4월의 측정자료를 사용하였다. 그 결과, 신곡보 철거시 수온의 변화는 서해로부터의 차가운 해수의 역류 증가로 임진강, 파주, 전류, 장항습지까지 수온이 최대 $0.94^{\circ}C$ 크게 하강할 것으로 나타났으나 행주, 가양, 영등포는 오히려 수온이 $0.03{\sim}0.04^{\circ}C$ 미미하게 상승할 것으로 나타났으며 현재의 신곡보가 수온의 급격한 변화를 완층하는 역할을 담당하여 왔으나 보가 철거되면서 이러한 수온 완충역할이 사라지기 때문으로 분석되었다. 신곡보 철거시 BOD 농도의 변화는 잠실보 하류쪽으로는 BOD 농도가 최대 1.02 mg/l 감소하는 등 전반적으로 수질이 크게 개선될 것으로 나타나는 반면 잠실보 상류쪽으로는 BOD 농도가 최대 0.56 mg/l 증가하며 현재의 1급수 수질에서 2급수로 수질이 악화되어 상수원 수질 관리가 어려울 것으로 예상되었다. 신곡보 철거시 조류 농도의 변화는 잠실보 하류쪽으로 조류 농도가 최대 1.85 mg-A/l 감소하는 등 전반적으로 수질이 크게 개선될 것으로 나타나는 반면 잠실보 상류쪽으로는 조류 농도가 최대 0.40 mg-A/l 증가하며 고조위 기간에는 조류주의보 기준 1.5 mg-A/l를 초과하는 경우도 발생하여 상수원 취수에 문제가 발생할 가능성이 있는 것으로 나타났다.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.2
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• pp.225-241
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• 2007

A closed flux chamber system was used for measuring major greenhouse gas (GHG) emission from tideland and/or wetland soils in estuarine area at Saemankum, Kunsan in southwestern Korea during from months of February to June 2006. Hourly averaged GHG soil emissions were measured two to three times a day during the ebb tide hours only. Site soils were analyzed for soil parameters (temperature, pH, total organic contents, N and C contents in soil) in the laboratory. Soil GHG fluxes were calculated based on the GHG concentration rate of change measured inside a closed chamber The analysis of GHG was conducted by using a Gas Chromatography (equipped with ECD/FID) at laboratory. Changes of daily, monthly GHGs' fluxes were examined. The relationships between the GHG emissions and soil chemical contents were also scrutinized with respect to gas production and consumption mechanism in the soil. Soil pH was pH $7.47{\pm}0.49$ in average over the experimental period. Organic matter contents in sample soil was $6.64{\pm}4.98\;g/kg$, and it shows relatively lower contents than those in agricultural soils in Kunsan area. Resulting from the soil chemistry data, soil nitrogen contents seem to affect GHG emission from the tidal land surface. The tidal soil was found to be either source or sink for the major GHG during the experimental periods. The annual average of $CH_{4}\;and\;CO_{2}$ fluxes were $0.13{\pm}0.86\;mg\;m^{-2}h^{-1}\;and\;5.83{\pm}138.73\;mg\;m^{-2}h^{-1}$, respectively, which will be as a source of these gases. However, $N_{2}O$ emission showed in negative flux, and the value was $-0.02{\pm}0.66\;mg\;m^{-2}h^{-1}$, and it implies tidal land surface act as a sink of $N_{2}O$. Over the experimental period, the absolute values of gas fluxes increased with soil temperature in general. Averages of the ambient gas concentration were $86.8{\pm}6.\;ppm$ in $CO_{2},\;1.63{\pm}0.34\;ppm\;in\;CH_{4},\;and\;0.59{\pm}0.15\;ppm\;in\;N_{2}O$, respectively. Generally, under the presence of gas emission from agricultural soils, decrease of gas emission will be observed as increase in ambient gas concentration. We, however, could not found significant correlation between the ambient concentrations and their emissions over the experimental period. There was no GHG compensation points existed in tide flat soil.