• 제목/요약/키워드: Methyl phosphate

검색결과 105건 처리시간 0.022초

Effect of Bacillus subtilis S37-2 on Microorganisms in Soil and Growth of Lettuce (Lactuca sativa)

  • Heo, Jae-Young;Kim, Dae-Ho;Choi, Yong-Jo;Lee, Sang-Dae;Seuk, Su-Won;Song, Jae-Kyeong;Kwon, Jang-Sik;Kim, Min-Keun
    • 한국토양비료학회지
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    • 제49권5호
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    • pp.621-626
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    • 2016
  • The present study evaluated the variations in soil microbial population of controlled horticultural land used for lettuce (Lactuca sativa) cultivation by their fatty acid methyl ester and chemical properties. We utilized four treatment groups, no treatment (NT), culture medium (CM), Bacillus subtilis S37-2 (KACC 91281P) ${\times}10^6CFU\;mL^{-1}$ (BS1), and Bacillus subtilis $S37-2{\times}10^7CFU\;mL^{-1}$ (BS2) and analyzed these variations throughout the before treatment and harvesting stage. The chemical properties such as pH, organic matter, available phosphate, and electrical conductivity in soils before treatment and harvesting stage showed no significant difference among the treatments. Total numbers of bacteria and microbial biomass C in soil treated with BS1 were larger than those of NT, CM, and BS2, whereas total number of fungi at the harvesting stage was significantly lower in the BS1 soil than in the NT and CM soils (P < 0.05). On basis of leaf length, leaf width, leaf number and leaf weight, the growth characteristics lettuce on the soil treated with BS1 and BS2 was faster than those of NT and CM soils. Yield of lettuce with treated BS1 and BS2 were 35% and 29% more than that of NT, respectively.

Phenazine and 1-Undecene Producing Pseudomonas chlororaphis subsp. aurantiaca Strain KNU17Pc1 for Growth Promotion and Disease Suppression in Korean Maize Cultivars

  • Tagele, Setu Bazie;Lee, Hyun Gu;Kim, Sang Woo;Lee, Youn Su
    • Journal of Microbiology and Biotechnology
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    • 제29권1호
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    • pp.66-78
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    • 2019
  • In this study, strain KNU17Pc1 was tested for its antifungal activity against Rhizoctonia solani AG-1(IA), which causes banded leaf and sheath blight (BLSB) of maize. KNU17Pc1 was tested further for its broad-spectrum antifungal activity and in vitro plant growth promoting (PGP) traits. In addition, the in vivo effects of KNU17Pc1 on reduction of BLSB severity and seedling growth promotion of two maize cultivars under greenhouse conditions were investigated. On the basis of multilocus sequence analysis (MLSA), KNU17Pc1 was confirmed as P. chlororaphis subsp. aurantiaca. The study revealed that KNU17Pc1 had strong in vitro antifungal activity and was effective toward all in vitro PGP traits except phosphate solubilization. In this study, for the first time, a strain of P. chlororaphis against Colletotrichum dematium, Colletotrichum gloeosporioides, Fusarium oxysporum f.sp. melonis, Fusarium subglutinans and Stemphylium lycopersici has been reported. Further biochemical studies showed that KNU17Pc1 was able to produce both types of phenazine derivatives, PCA and 2-OH-PCA. In addition, solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis identified 13 volatile organic compounds (VOCs) in the TSB culture of KNU17Pc1, 1-undecene being the most abundant volatile. Moreover, for the first time, Octamethylcyclotetrasiloxan (D4), dimethyl disulfide, 2-methyl-1,3-butadiene and 1-undecene were detected in P. chlororaphis. Furthermore, this study reported for the first time the effectiveness of P. chlororaphis to control BLSB of maize. Hence, further studies are necessary to test the effectiveness of KNU17Pc1 under different environmental conditions so that it can be exploited further for biocontrol and plant growth promotion.

Engineered Recombinant PON1-OPH Fusion Hybrids: Potentially Effective Catalytic Bioscavengers against Organophosphorus Nerve Agent Analogs

  • Lee, Nari;Yun, Hyeongseok;Lee, Chan;Lee, Yikjae;Kim, Euna;Kim, Sumi;Jeon, Hyoeun;Yu, Chiho;Rho, Jaerang
    • Journal of Microbiology and Biotechnology
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    • 제31권1호
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    • pp.144-153
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    • 2021
  • Organophosphorus nerve agents (OPNAs), including both G- and V-type nerve agents such as sarin, soman, tabun and VX, are extremely neurotoxic organophosphorus compounds. Catalytic bioscavengers capable of hydrolyzing OPNAs are under development because of the low protective effects and adverse side effects of chemical antidotes to OPNA poisoning. However, these bioscavengers have certain limitations for practical application, including low catalytic activity and narrow specificity. In this study, we generated a fusion-hybrid form of engineered recombinant human paraoxonase 1 (rePON1) and bacterial organophosphorus hydrolase (OPH), referred to as GV-hybrids, using a flexible linker to develop more promising catalytic bioscavengers against a broad range of OPNAs. These GV-hybrids were able to synergistically hydrolyze both G-type OPNA analogs (paraoxon: 1.7 ~ 193.7-fold, p-nitrophenyl diphenyl phosphate (PNPDPP): 2.3 ~ 33.0-fold and diisopropyl fluorophosphates (DFP): 1.4 ~ 22.8-fold) and V-type OPNA analogs (demeton-S-methyl (DSM): 1.9 ~ 34.6-fold and malathion: 1.1 ~ 4.2-fold above) better than their individual enzyme forms. Among the GV-hybrid clones, the GV7 clone showed remarkable improvements in the catalytic activity toward both G-type OPNA analogs (kcat/Km (106 M-1 min-1): 59.8 ± 0.06 (paraoxon), 5.2 ± 0.02 (PNPDPP) and 47.0 ± 6.0 (DFP)) and V-type OPNA analogs (kcat/Km (M-1 min-1): 504.3 ± 48.5 (DSM) and 1324.0 ± 47.5 (malathion)). In conclusion, we developed GV-hybrid forms of rePON1 and bacterial OPH mutants as effective and suitable catalytic bioscavengers to hydrolyze a broad range of OPNA analogs.

산지초지 개량에 관한 연구 III. 종자 Coating에 의한 겉뿌림 초지육성 (Studies on the Improvement of Mountainous Pasture III. Effect of seed coating on oversown pasture)

  • 이인덕
    • 한국초지조사료학회지
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    • 제4권3호
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    • pp.194-200
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    • 1984
  • 종자(種子) coating이 겉뿌린 목초(牧草)의 정착개체수(定着個體數), 초기생육(初期生育) 및 수량(收量)에 미치는 영향을 구명(究明)코저 60% lime, 20% Phosphate 및 20% peatmoss(w/w)의 증량피복재료(增量被覆材料)와 13% arabic gum및 2% methyl cellulose (w/v)를 접착제(接着劑)로 사용(사용)하여 종자(種子)와 피복재료(被覆材料)의 무게 44를 1 : 20(w/w)으로 coating하여 pot, soil box 및 field에서 시험(試驗)한 결과(結果)는 다음과 같다. 1. 종자(種子) coating은 겉뿌린 수준(水準)의 초기(初期) 생육(生育)을 촉진(促進)하였다(Table 2와 3). 2. 종자(種子) coating은 겉뿌린 목초(牧草)의 초기(初期) 정착개체수(定着個體數)를 15%정도 증가시켰고, orchardgrass, perennial ryegrass 및 kentucky bluegrass의 정착개체수(定着個體數)를 증가시킨 반면 대립종(大粒種)의 tall fescue 및 소립종(小粒種)의 ladino clover에는 큰 차이(差異)가 없었다. 3. 종자(種子) coating은 겉뿌린 목초(牧草)의 식생비율(植生比率)을 증가시켰다. 4. 종자(種子) coating은 겉뿌림 초지(草地)의 조성당년(造成當年)의 건물수량(乾物收量)을 17%정도 증가시켰다(P<0.05). 5. 종자(種子) coating시(時) 증량피복물질(增量被覆物質)은 토양(土壤) 및 식물체(植物體)의 화학성분(化學成分)에 영향을 주었다(Table 8). 6. 환경조건이 불량(不良)한 지역(地域)에서의 겉뿌림 초지개량시(草地改良時) 종자(種子) coating은 관행적(慣行的)인 겉뿌림 방법보다 목초(牧草)의 정착개체수(定着個體數), 초기생육(初期生育) 및 수량(收量)을 증가시킬 수 있는 확실한 방법(方法)이라 할 수 있다.率)과의 관계를 주의깊게 고려해야 할 것이다. 생산(生産)할 수 있다고 사료(思料)된다.향이 없었으나 골목 $m^3$당 접종량(接種量) 4.0kg구가 1.63g로 가장 큰 것으로 나타났다.과(結果)를 종합(綜合)해 보면 전호(前胡)의 용도별(用途別) 적정(適正) 재식거리(栽植距離)는 채소형(菜蔬型) $30\times20cm$, 약재형(藥材型) $60\times30cm$, 채소(菜蔬) 약재(藥材) 복합형(複合型) $45\times20cm$에서 지하부(地下部) 생육(生育)이 양호(良好)하면서 생엽(生葉) 및 건근(乾根) 수량(收量)이 증수(增收)되는 경향(傾向)을 보였다.퇴비(堆肥) 등(等) 유기질(有機質) 비료(肥料) 시용(施用)이 효고적(效果的)이었다.DS-PAGE법(法)으로 단백질(蛋白質) 양상을 등숙시기별(等熟時期別)로 조사(調査)한 결과(結果) 두 계통간(系統間) 질적(質的)인 차이(差異)는 보이지 않았으나 시기별(時期別)로는 양적차이(量的差異)를 나타냈다.間)에는 부(負)(-)의 상관(相關)이 있다.($P{\leq}0.01%$). 5. NEL 및 starch value 환경온도(環境溫度)가 상승(上昇)됨에 따라 감소(減少)된다. 4 엽기(葉期) sorghum식물(植物)의 환경온도(環境溫度)를 달리 하였을 때 NEL가치(價値)는 각각(各各) 4.87MJ($30/25^{\circ}C$), 5.46MJ($25/20^{\circ}C$) 및 5.81MJ/kg($18/8^{\circ}C$)로 변(變)하여 고온(高溫)에서 net energy lactation 축적(蓄積)이 크게 감소(減少)되었다.다. 그러나 기온(氣溫)이 낮은 조건(條件)에서는 예취관리(刈取管理)에 따른 비구조성(非構造性) 탄수화물(炭水化物)의 함량변화(含量變化)

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Complete Freund Adjuvant에 의한 피부염증에서 통각과민현상의 기전 (Mechanism of Hyperalgesia Following Cutaneous Inflammation by Complete Freund Adjuvant)

  • 정용;임중우;정승수;김윤숙;윤덕미;남택상;백광세
    • The Korean Journal of Pain
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    • 제13권2호
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    • pp.164-174
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    • 2000
  • Background: After an injury to tissue such as the skin, hyperalgesia develops. Hyperalgesia is characterized by an increase in the magnitude of pain evoked by noxious stimuli. It has been postulated that in the mechanism of hyperalgesia (especially secondary hyperalgesia) and allodynia, a sensitization of central nervous system such as spinal dorsal horn may contribute to development of hyperalgesia. However, the precise mechanism is still unclear. In the present study, we investigated the roles of N-methyl-D-aspartate (NMDA) receptor and nitric oxide (NO) system in the mechanism of hyperalgesia, and their relations with c-fos expression Methods: Inflammation was induced by injection of complete Freund adjuvant (CFA) into unilateral hindpaw of Sprague-Dawley rat. Behavioral studies measuring paw withdrawal responses by von Frey filaments and paw withdrawal latencies by radiant heat stimuli and stainings of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase and c-fos immunoreactivity were performed. The effects of MK-801, an NMDA receptor blocker and $N^\omega$-nitro-L-arginine (L-NNA), a nitric oxide synthase (NOS) inhibitor were evaluated. Results: 1) Injection of CFA induced mechanical allodynia, mechanical hyperalgesia and thermal hyperalgesia. And it increased the number of NADPH-diaphorase positive neurons and c-fos expression neurons. 2) MK-801 inhibited mechanical hyperalgesia and thermal hyperalgesia induced by CFA and reduced the number of NADPH-diaphorase positive neurons and c-fos expression neurons. 3) L-NNA inhibited the thermal hyperalgesia and reduced the number of NADPH-diaphorase positive neurons, but did not affect the number of c-fos expression neurons. Conclusions: These results suggest that in the mechanism of mechanical hyperalgesia, NMDA receptor but not NO-system is involved and in the case of thermal hyperalgesia both NMDA receptor and NO system are involved. NO system did not affect the expression of c-fos, but c-fos expression and NOS activity were dependent on the activity of NMDA receptor.

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농산폐기물(農産廢棄物)에서 발효사료(醱酵使料)의 생산(生産)에 관(關)한 연구(硏究) [제1보(第一報)] -Aspergillus niger에 의(依)한 Xylanase의 생산(生産) 및 그 효소특성(酵素特性)에 관(關)하여- (Studies on the Production of Fermented Feeds from Agricultural Waste Products [Part 1] -On the Production and Characteristics of Xylanase by Aspergillus niger-)

  • 이계호;이형주
    • Applied Biological Chemistry
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    • 제18권3호
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    • pp.109-116
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    • 1975
  • 농산폐기물을 이용할 목적으로 8점의 시료에서 xylanase생산성이 우수한 두개의 균주를 선발하여 동정하였다 선정된 두 균주에 대해 xylanase의 생산 최적조건효소의 제 특성을 조사하여 다음과 같은 결과를 얻었다. 1. Xylanase생산에 우수한 균주로 Aspergillus niger-1701과 Aspergillus niger-430 두 균주를 선발하였다. 2. 두 균주 모두 배양시 pH $5.0{\sim}6.0$에서 배양 2일째에 최고의 효소역가를 나타내었다. 3. Aspergillus niger-1701균주에 대해서는 배양시 첨가한 C.M.C.가 21%, $NH_4H_2PO_4$가 19%, C.S.L.이 20%의 효소생산 증가를 나타냈으며 기타 탄소원, 질소원, 인산염, 생장요소, 무기염류 등은 큰 영향을 미치지 않았다. 4. Aspergillus niger-430균주에 대해서는 배양시 첨가한 탄소원, 질소원, 인산염, 생장요소, 무기염류 등이 효소생산에 큰 영향을 미치지 않았다. 5. 선정된 두 균주가 생산한 xylanase의 효소작용 최적 조건은 다음과 같다. pH:5.0 온도 : $60^{\circ}C$, 효소농도 : 33.3%(전용액중), 기질농도 : 2%, 효소작용시간 : 3 hrs. 6. 효소작응 최적조건에서 xylan최고 분해율은 95%였다.

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개 하부식도괄약근의 비아드레날린성, 비콜린성 이완반응에 있어서 Cyclic Nucleotide의 역할 (Regulatory Role of Cyclic Nucleotides in Non-Adrenergic Non-Cholinergic Relaxation of Lower Esophageal Sphincter from Dogs)

  • 김영태;임병용
    • The Korean Journal of Physiology and Pharmacology
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    • 제1권3호
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    • pp.303-313
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    • 1997
  • The role of the lower esophageal sphincter(LES) is characterized by the ability to maintain tone and to relax allowing the passage of a bolus. It is known that LES relaxation during swallowing may be induced by the cessation of the tonic neural excitation and the activation of non-adrenergic, non-cholinergic(NANC) inhibitory neurons. Furthermore, it is generally accepted that the relaxation of the smooth muscle is mediated primarily by the elaboration of adenosine 3',5'-cyclic monophosphate(cyclic AMP) and guanosine 3',5'-cyclic mono-phosphate(cyclic GMP) via activation of adenylate cyclase and guanylate cyclase, respectively. It is thus possible that cyclic nucleotides might be a second messenger involved in neural stimulation-induced relaxation of LES, although a relationship between relaxation and changes in cyclic nucleotides after neural stimulation has not been established. The present study was performed to define the participation of cyclic nucleotides in the relaxation of LES of dog in response to neural stimulation. Electrical field stimulation(EFS) caused relaxation of the canine isolated LES strips in a frequency-dependent manner, which was eliminated by pretreatment with tetrodotoxin$(1{\mu}M)$, but not by atropine$(100{\mu}M)$, guanethidine$(100{\mu}M)$ and indomethacin$(10{\mu}M)$. The nitric oxide synthase inhibitors, $N^G-nitro-L-arginine$, $N^G-nitro-L-arginine$ methyl ester and $N^G-monomethyl-L-arginine$ inhibited EFS-induced relaxation. Additions of sodium nitroprusside, a nitrovasodilator and forskolin, a direct adenylate cyclase stimulant, caused a dose-dependent relaxation of LES smooth muscle. Effects of sodium nitroprusside and forskolin were selectively blocked by the corresponding inhibitors, methylene blue for guanylate cyclase and N-ethylmaleimide(NEM) for adenylate cyclase, respectively. Dibutyryl cyclic AMP and dibutyryl cyclic GMP caused a concentration-dependent relaxation of the LES smooth muscle tone, which was not blocked by NEM or methylene blue, respectively. However, both NEM and methylene blue caused significant antagonism of the relaxation in LES tone in response to EFS. EFS increased the tissue cyclic GMP content by 124%, whereas it did not affect the tissue level of cyclic AMP. Based on these results, it is suggested that one of the components of canine LES smooth muscle relaxation in response to neural stimulation is mediated by an increase of cyclic GMP via the activation of guanylate cyclase. Additionally, an activation of cyclic AMP generation system was, in part, involved in the EFS-induced relaxation.

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양념 소갈비의 조리과정에서의 물리화학적 특성 평가 (The Physicochemical Characteristics of Marinated Beef Galbi under Different Cooking Conditions)

  • 홍상필;김영호;이남혁;허영욱
    • 한국식생활문화학회지
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    • 제28권1호
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    • pp.78-88
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    • 2013
  • Marinated beef galbi is a traditional Korean dish cooked with soy sauce, pear juice, onion, sesame oil, and sugar. However, there are many differences in beef galbi, including flavor and physicochemical aspects, depending on cooking conditions. Therefore, the physicochemical characteristics of marinated beef galbi prepared through various recipes was evaluated for its effects on pH, texture, aging, proteolysis, heating conditions, cooking time, and flavor compounds (pyrazines, IMPs, or FAAs). There were significant differences in salt concentration (0.8~3.03%), pH (4.89~6.22), and solid soluble contents (1.34-6.31 Brix) between recipes in this study. In the Pearson assay for sensory evaluation, overall preference correlated well with texture (a well-known sensory attribute in meat evaluation). Controlling the pH of meat through soaking in lemon solution, alkali water, phosphate, and baking powder solution, improved water holding capacity as much as 9 to 15% compared with the control. The myofibril index (MFI) of marinated meat stored at $4^{\circ}C$ increased 32% with 24 hours of aging and reached 39% at 48 hours of aging, and its fragmentation was observed through microscopy. SDS-PAGE showed hydrolysis of acid-soluble collagen by the pear juice, possibly related to meat tenderness. On the basis of surface temperature, the cooking time was estimated to be 8 minutes with pan heating at $170^{\circ}C$, 6 minutes at $270{\sim}300^{\circ}C$, and 4 minutes with charcoal at $700{\sim}900^{\circ}C$. Different pyrazine compounds, such as 2-methyl-3-phenylpyrrol(2,3-b) pyrazine (the typical product of the browning reaction) was mainly detected, and IMP (one of the main taste compounds in beef) was in higher amounts with the charcoal treatment, potentially related to its flavor preference among treatments. Our results demonstrate an effective case study and cooking system for beef galbi.

Immunoblot를 이용한 폐흡충 비항원의 특이 항원대의 증명 (Demonstration of species-specific and cross reactive components of Paragonimus tvestermani crude worm antigen by EITB)

  • 주경환;안혁;정명숙;임한종
    • Parasites, Hosts and Diseases
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    • 제27권1호
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    • pp.9-14
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    • 1989
  • 폐흡충중의 진단은 일반적으로 객담에서 충란을 검출하는 것이 가장 확실한 진단법으로 간주되고 있지만 이소기생의 경우는 물론, 본 기생충의 감염자 및 감염 강도의 감소로 인해 실제 폐에 기생한 경우에 있어서도 객담 검사의 민감도가 매우 낮아져 혈청학적 검사의 필요성이 점차 대두되고 있다. 따라서 식염수 추출액을 항원으로 하여 효소면역 측정법을 실시하는 방법으로 면역 진단을 실시하고 있으나 조항원의 단백 구성이 복잡하여 간흡충을 비롯한 몇몇 흡충류 감염자 혈청에서 교차 반응이 야기되고 있다. 그동안 정제 항원을 만들고자 하는 시도가 많았음에도 불구하고 특이 항원의 분리는 실제로 불가능하였다. 이와 같은 문제점을 보완하기 위하여 폐흡충 조항원을 SDS-PAGE로 전기영동한 다음 EITB를 이용하여 항원대별 항원성 및 특이성을 관찰하여 폐흡충 조항원의 폐흡충증 혈청에 대한 특이 반응대를 확인해 보고자 하였다. 실험에 사용된 항원은 실험적으로 개에 감염시켜 24주만에 얻은 폐흡충 성충의 식염수 추출액이며 3∼20%의 linear gradient gel에서 SDS-PAGE하였다. Silver 염색한 결과 229 kDa∼10kDa 사이에서 26개의 항원대를 구성하고 있었으며 주요 항원대에 대하여 EITB를 실시한 결과 폐흡충 감염자 혈청은 229, 91, 60, 50, 35∼31, 27, 25, 21, 17, 11 및 10kDa의 분자량을 갖는 항원대와 반응하였다. 간흡충 감염자 혈청은 35∼31, 19, 11및 17 kDa의 항원대와 반응하였고 낭미충 감염자 혈청은 229, 31∼31, 27, 25 및 17kDa의 항원대와 반응하였다. 광절열두조충 1예도 17 kDa의 항원대와 반응하였다. 따라서 91, 60, 21 및 10kDa의 항원대 중 일부가 폐흡충 조항원에 있어서 체홉충에 대한 종특이 항원대로 생각되었으며 향후 immunoblot을 이용한 폐흡충중의 면역진단에 이용될 수 있는 항원대로 간주할 수 있었다.

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계면활성제 용액속에서의 화학반응(제3보) 유기인산 에스테르의 가수분해반응에 미치는 2-알킬벤즈이미다졸-5-술포네이트의 친핵적 및 미셀 촉매효과 (Chemical Reactions in Surfactant Solutions(Ⅲ). Nucleophilic and Micellar Catalyses on Hydrolysis of an Organic Phosphate by Sodium 2-Alkylbenzimidazole-5-sulfonates in Aqueous and CTABr Solutions)

  • 홍영석;박희현;박한석
    • 대한화학회지
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    • 제34권6호
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    • pp.629-636
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    • 1990
  • CTABr 미셀 용액속에서의 sodium 2-alkylbenzimidazole-5-sulfonate(R-BISO$_3$Na)의 음이온에 의해 추진되는 p-nitrophenyldiphenylphosphate(p-NPDPP)의 탈인산화 반응은 sodium benzimidazole-5-sulfonate(BI-SO$_3$Na)의 음이온에 의해 추진되는 반응의 반응속도보다 현저히 감소하고, 알킬기의 길이가 methyl기에서 heptyl기로 길어질수록 감소의 정도가 더욱 크다. 이것은 CTABr을 포함하고 있지 않는 수용액속에서의 BI-SO$_3$Na 및 R-BI$^-$SO$_3$Na 에 의한 탈인산화 반응속도가 별차이가 없음을 감안할 때, 이들 친핵체의 알킬기가 미셀 의사층(micellar pseudophase)내에서 입체장애(steric hinderance)로 작용하기 때문인 것으로 판단된다. 이것은 수용액과 미셀 용액속에서의 반응의 측정된 활성화에너지(△H$^\neq$/TEX>, △G$^\neq$/TEX> 및 △S$^\neq$/TEX>)의 값과도 정성적으로 일치하고 있다. 이러한 입체장애가 반응속도에 미치는 영향을 정량적으로 계산하고자 시도하였다. Nonyl기에서 pentadecyl기까지 긴 알킬기를 갖고 있는 R-BI$^-$SO3Na는 그것들의 benzimidazole 부분(BI moiety)이 친핵체로 작용할 뿐 아니라, 이 분자들은 CTABr을 포함하지 않는 수용액속에서 미셀을 형성하여 반응을 촉진함을 알았다.

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