• Journal of the Society of Cosmetic Scientists of Korea
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• v.15 no.1
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• pp.71-76
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• 1989

• Journal of Food Hygiene and Safety
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• v.11 no.1
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• pp.17-24
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• 1996

The genotoxicity of combinations of four p-oxybenzoic acids (methyl paraben, ethyl paraben, isopropyl paraben, butyl paraben) and benzoic acid had been evaluated. The in vitro Ames test using Salmonella typhimurium (TA 98, TA 100, 1535, TA 1537) and the invivo micronucleus assay using mouse peripheral blood were performed. Methyl paraben plus benzoic acid, ethyl paraben plus benzoic acid, and ethl paraben plus butyl paraben slightly increased the frequency of microuncleated reticulocytes in the high doses, but were negative in Ames test with Salmonella typhimurium with and without rat liver microsomal activation. The other combinations tested were negative in Ames test and did not show any clastogenic effect in micronucleus test. These results suggest that genotoxicity can produced by th combination of p-oxybenzoic acid.

• Journal of the Korean Applied Science and Technology
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• v.27 no.4
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• pp.501-507
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• 2010

In this study, we evaluate the anti-microbiological activity of paraben in eye shadows that are composed of pigments and oil binders using various analytical methods and microbiological tests. Paraben does not show the microbiological activity properly when it was used with Nylon SP$^{(R)}$ 10, Talc RF SSA$^{(R)}$, OMC Talc AS$^{(R)}$ and $BaSO_4$. In the test of fungi, Nylon SP$^{(R)}$ 10 causes the decrease of microbiological activity regardless of the type of oil binders. The pigment of Mango violet also causes the decrease of microbiological activity when ester oil binder was used. Regardless of the type of oil binder, samples containing nylon SP 10, 0.15% of methyl paraben and 0.05% of propyl paraben had not been able to maintain microbiological activity only if the concentration of parabens were increased. Trace amounts of metal ions present in pigments reduced the activity of preservatives by inactivation of hydroxyl group of paraben. It is thought that swollen nylon SP 10 in ester oil increase the absorption or interaction of parabens and swollen nylon powder causes the inactivation of paraben.

• Korean Journal of Microbiology
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• v.19 no.3
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• pp.103-107
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• 1981

Increasing the concentration of the nonionic surfactants the solubility of methyl paraben was increased. This is called the solubilization phenomenon and caused inactivation of the preservatives used. The MICs(minimum inhibitory concentrations) on E. coli were increased at the same time. So the relation between the solubility and the mic could be expressed as $S-S_0=R^{\prime}/R^{\prime\prime}\;(M-M_0)$ and in this case $R^{\prime}/R^{\prime\prime}$ was about 2.

• Applied Chemistry for Engineering
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• v.29 no.5
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• pp.519-523
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• 2018

Cyclodextrins are a class of oligosaccharides having an extremely low toxicity, so that they have been used for the formation of host-guest complexes and removal of toxic gases or molecules. In this study, the subtle phenomenon associated with the formation of host-guest complexes between cyclodextrin and toxic molecules (methyl paraben) was experimentally investigated. First, the formation of cyclodextrin-methyl paraben complexes was monitored by UV/Vis spectroscopy as a function of the cyclodextrin concentration. Secondly, the electrochemical measurement was performed with the surface engineered Au electrode having cyclodextrin molecules on the Au substrate. The sensing signal derived from the addition of methyl paraben solution into the Au surface was measured delicately. This study can be informative for future applications such as sensors.

• Analytical Science and Technology
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• v.31 no.6
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• pp.225-231
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• 2018

Benzoic acid, methyl paraben, and butyl paraben are preservatives that have been used in pharmaceutical, cosmetic, and food products. However, as their toxicities for human have been reported, many nations and organizations including Korea have established a regulation limit for thier usage of these preservatives in food products. The present study developed the isotope dilution liquid chromatography mass spectrometry method for accurate determination of three target preseratives in soysauce. In this study, the isotope dilution liquid chromatography mass spectrometry method was developed for accurate determination of three target preservatives in soy sauce. LC separation was optimized considering the pKa of benzoic acid which is lower than those of methyl and butyl parabens. A C18 column was used with 5 mM ammonium acetate and methanol as mobile phases. Mass spectrometry was operated in negative mode and selected reaction monitoring mode (SRM). Soy sauce sample was cleaned-up with C18 SPE cartridge for removing matrix inferences and color material. Optimized conditions and the method were validated with soy sauce reference materials for the analysis of food preservatives from Health Science Authority in Singapore. The measured values of benzoic acid, methyl and butyl paraben agreed well with reference values within their uncertainties.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.54-63
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• 2019

Rubus coreanus Miquel (RCM), also known as Korean blackberry or bokbunja, is used as a South Korean traditional medicine to treat acne and inflammatory skin conditions. The antimicrobial activity of RCM root and its active compounds remain unclear. In this study, we prepared a 50% ethanol fraction, ethyl acetate fraction, and acid-treated ethyl acetate fraction (aglycone fraction) of RCM root, and evaluated antibacterial activities against the skin pathogens Staphylococcus aureus, Pseudomonas acnes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. In a paper disc assay, all fractions of RCM root showed antimicrobial activities against the five skin pathogens. The ethyl acetate fraction displayed 6-, 12-, and 2-fold higher minimal inhibitory concentration (MIC) than the 50% ethanol fraction against S. aureus, E. coli, and P. acnes, respectively. The aglycone fraction displayed 2-fold higher MIC than methyl paraben against P. acnes, S. aureus, E. coli, and P. aeruginosa. The ethyl acetate fraction displayed a minimal bactericidal concentration (MBC) similar to that of methyl paraben, and the aglycone fraction showed 2- to 4-fold higher MBCs than those of methyl paraben. In particular, the ethyl acetate fraction was not cytotoxic and showed thermal stability after incubation at high temperatures ($60-121^{\circ}C$). Finally, the ethyl acetate fraction was separated and four components were identified: procyanidin C, propelagonidin dimer, ellagic acid, and methyl ellagic acid acetyl pentose. The compounds showed high antibacterial activities. These results suggest that RCM root is potentially applicable as a natural preservative in cosmetics.

• Journal of Environmental Health Sciences
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• v.49 no.1
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• pp.37-47
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• 2023

Background: Paraben is a widely used substance with a preservative effect found in various materials such as food, medicine, personal care products, and cosmetics. Objectives: This study was conducted to identify the level of urinary paraben concentrations (i.e., methyl-, ethyl-, and propyl-) among Korean adults and to explore the factors related with the exposure levels. Methods: We analyzed the third period (2015~2017) of the Korean National Environmental Health Survey (KoNEHS). R statistical software (version 4.1.1) was used to estimate representative values for the whole population with weight variables to reflect sampling design. Whether urinary concentrations tended to increase as the level of paraben exposure-related characteristics increased was tested and P_trend was calculated using general linear models. Results: Urinary concentrations of all three parabens (i.e., methyl-, ethyl- and propyl-) were higher in women than in men (P_trend<0.0001, 0.008, and <0.0001), and the values of methylparaben and propylparaben tended to increase as the age of subjects increased (P_trend<0.0001, and <0.0001). Urinary concentrations of methylparaben and propylparaben were associated with intensity of exercise (P_trend<0.001, and 0.004), and that of propylparaben was higher in non-smokers (P_trend=0.01). In terms of paraben exposure-related variables, urinary concentrations of parabens (i.e., methyl-, ethyl- and propyl-) increased as the daily average frequency of teeth-brushing (P_trend<0.0001, 0.03 and 0.0001), the frequency of use of hair products (P_trend=0.005, 0.05 and 0.04), the frequency of use of makeup products (P_trend<0.001, 0.001 and <0.001), and the frequency of use of antibacterial products (P_trend=0.005, 0.02 and 0.02) increased. Conclusions: In our study, urinary concentrations of all three parabens are associated with gender, teethbrushing, hair products, make-up products, and antibacterial products. Methyl- and proyl-parabens were associated with age and intensity of exercise, and propyl-paraben was associated with smoking.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.3
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• pp.219-227
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• 2015

This study was conducted to determine 10 preservatives (benzyl alcohol (BAl), phenoxyethanol (PE), benzoic acid (BA), sorbic acid (SA), methyl paraben (MP), ethyl paraben (EP), propyl paraben (PP), isopropyl paraben (IPP), butyl paraben (BP), isobutyl paraben (IBP)) levels in 125 cosmetics (n = 125) for children by the simultaneous analysis of HPLC. The detection ranges were as follows; 0.01 ~ 0.91% (n = 35) for PE, 0.01 ~ 0.48% (n = 28) for BA, 0.01 ~ 0.78% (n = 9) for BAl, 0.01 ~ 0.11% (n = 3) for SA, 0.04 ~ 0.21% (n = 8) for MP, 0.02 ~ 0.09% (n = 8) for PP, and 0.04% (n = 1) for EP. The order of detection rates was cleanser (63%) > cream (48%) > sunscreen (46%) > lotion (38%) > oil (13%). At least one of target preservatives was contained in 50% (63/125) of samples and the content of the detected preservatives was within maximum allowed amount established by KFDA. Phenoxyethanol and benzoic acid were used more frequently than paraoxybenzoate esters (parabens) in cosmetics for children and the detected parabens was mainly the mixture of methyl paraben and propyl paraben.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.7 no.1
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• pp.40-43
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• 1979