• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.597-603
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• 1996

This study was carried out to examine degradation characteristics of microalgae Chlorella vulgaris in methane fermentation. We measured COD and VS reduction, gas and methane productivity, VFA (volatile fatty acid), respectively. Then we calculated material balance and hydrolysis rates in soluble and solid material. The substrate concentration was controlled from 14 gCOD$_{cr}$/l to 64 gCOD$_{cr}$/l in batch cultures, and HRT (hydraulic retention time) controlled from 2 days to 30 days in continuous experi- ments. The results were as follows. In batch culture, accumulated gas productivity increased with the increase of the substrate concentration. The SS and VSS was removed all about 30% increase of substrate concentration and the most of the degradable material removed during the first 10 days. The curve of gas and methane production rate straightly increased until substrate concentration is 26 gCOD$_{cr}$/l. In continuous culture experiments, the removal rates at HRT 10days were 20% for total COD and TOC, respectively. At longer HRT, there was no increase in the removal efficiency. At HRT 15 days, the removal rates were 30% for SS and VSS, respectively. Soluble organic materials were rapidly degraded, and so there was no accumulated. Soluble COD concentration was not increase regardless of HRT-increasing. That meaned the hydrolysis was one of the rate-limiting stage of methane fermentation. The first-order rate constants of hydrolysis were 0.23-0.28 day$^{-1}$ for VSS, and 0.07-0.08 day$^{-1}$ for COD.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1426-1435
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• 2014

Saponins have been considered as promising natural substances for mitigating methane emissions from ruminants. However, studies reported that addition of saponin-rich sources often arrived at contrasting results, i.e. either it decreased methane or it did not. The aim of the present study was to assess ruminal methane emissions through a meta-analytical approach of integrating related studies from published papers which described various levels of different saponin-rich sources being added to ruminant feed. A database was constructed from published literature reporting the addition of saponin-rich sources at various levels and then monitoring ruminal methane emissions in vitro. Accordingly, levels of saponin-rich source additions as well as different saponin sources were specified in the database. Apart from methane, other related rumen fermentation parameters were also included in the database, i.e. organic matter digestibility, gas production, pH, ammonia concentration, short-chain fatty acid profiles and protozoal count. A total of 23 studies comprised of 89 data points met the inclusion criteria. The data obtained were subsequently subjected to a statistical meta-analysis based on mixed model methodology. Accordingly, different studies were treated as random effects whereas levels of saponin-rich source additions or different saponin sources were considered as fixed effects. Model statistics used were p-value and root mean square error. Results showed that an addition of increasing levels of a saponin-rich source decreased methane emission per unit of substrate incubated as well as per unit of total gas produced (p<0.05). There was a decrease in acetate proportion (linear pattern; p<0.001) and an increase in propionate proportion (linear pattern; p<0.001) with increasing levels of saponin. Log protozoal count decreased (p<0.05) at higher saponin levels. Comparing between different saponin-rich sources, all saponin sources, i.e. quillaja, tea and yucca saponins produced less methane per unit of total gas than that of control (p<0.05). Although numerically the order of effectiveness of saponin-rich sources in mitigating methane was yucca>tea>quillaja, statistically they did not differ each other. It can be concluded that methane mitigating properties of saponins in the rumen are level- and source-dependent.

• Journal of environmental and Sanitary engineering
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• v.15 no.2
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• pp.49-57
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• 2000

The objective of this study is to find solid waste decomposition in landfill without leachate discharge. This study was observed variation of landfill gas production rate and leachate for stabilization assessment, and using four sets of lysimeter as experimental apparatus. Soild waste decomposition was accelerated in without leachate discharge system by sufficient moisture for methane bacteria. And gas production rate was between 54.2ℓ/kg VS∼335.9ℓ/kg VS in each lysimeter. Generation time of methane gas was showed different in each lysimeter, but it was much faster than literature research. The time of stabilization phase were began as follows : L-1 400 day, L-2 350 day, L-3 170 day and L-4 70 day respectively. Decreasing times of BOD/COD ratio and C/N ratio were necessary more than literature research because organic matter was not discharge such as wash out.

• Journal of Wellbeing Management and Applied Psychology
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• v.6 no.2
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• pp.33-37
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• 2023

Purpose: Since 2013, marine dumping of wastewater has been banned, and research on eco-friendly and efficient land treatment has emerged. This study compared and tested changes in biogas production and anaerobic process efficiency depending on whether or not enzyme pretreatment was performed during anaerobic digestion from single-phase and two-phase to medium-temperature. Research design, data and methodology: The total sugar, direct sugar, pH, and acidity before and after fermentation were analyzed by G/C by anaerobic fermentation of the liquor wastewater, food wastewater 1, and food wastewater 2 at 30℃ for 67 hours, and the amount of methane gas generated was analyzed by balloon volume. Results: It was found that stable organic acid concentration and pH were found in the enzyme-treated food wastewater 2, and the amount of methane gas generated was also increased. Conclusions: When anaerobic digestion of the liquor wastewater and the food wastewater together, the performance of enzyme pretreatment resulted in increased digestive efficiency. It will be the basic data that can contribute to carbon neutrality and greenhouse gas reduction by increasing the production of biogas.

• Journal of Hydrogen and New Energy
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• v.19 no.6
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• pp.520-528
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• 2008

2-step methane reforming, consisting of syn-gas production and water splitting step, was carried out over Cu-ferrite/$ZrO_2$. To improve the reactivity over Cu-ferrite/$ZrO_2$ presenting low reactivity in 2-step methane reforming, the addition of Ni was considered. As the results, the added Ni to Cu-ferrite/$ZrO_2$ improved the reactivity in syn-gas production step. However, (Cu, Ni) ferrite/$ZrO_2$ showed carbon deposition in syn-gas production step when an excess Ni was added. Furthermore, (Cu, Ni) ferrite/$ZrO_2$ showed the high durability without the deactivation of the medium during repeated ten cycles, although it showed more deposited carbon than the medium without Ni.

• Microbiology and Biotechnology Letters
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• v.9 no.4
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• pp.219-223
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• 1981

Experiments on methane gas digestion were conducted to prevent livestock pollution and develop substitute energy. When about 30(w/w)% of sludge was added to cow feces, pig feces, and poultry feces and digested at 37$^{\circ}C$ for 20 days, methane gas produced per Kg of organic matter for cow feces, pig feces, poultry feces was 131, 248 and 235 l, respectively. pH decreased slightly at first but increased gradually afterwards during digestion period. When 20, 30, and 40(w/w)% of sludge were added to the mixture of cow feces (300g) and water (200g), the volumes of gas produced were 6.1, 14.5 and 13.4 l, respectively. Volume of methane gas produced from the mixture of cow feces and saw dust was much more than that from the mixture of cow feces and rice polishings. The contents of N, K, P for digestion residues were sufficient to be utilized as a fertilizer. When methane gas digestion was carried out with cow feces in a submersible pump digester the volume of methane gas produced per Kg of organic matter was 188 l. The price of total methane gas produced at this digestion was similar to that of the electric power consumed.

• Journal of the Korean Operations Research and Management Science Society
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• v.10 no.2
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• pp.54-64
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• 1985

Biomass conversion processes have the potential for satisfying approximately 25% of the national demand for methane gas. At the current time very littel analytical work has been done to optimally design and operate the production facilities associated with these processes. This study was motivated by the high cost of these proposed systems. The biomass in storage decays (exponentially) with time while the batch methane production rate decreases (exponentially) over time. The basic problem is to determine the optimal residence times for batches in the anaerobic degester to maximize total production over a fixed planning horizon. The analysis characteries the form of the optimal policy and presents efficient algorithm for obtaining this solution.

• Journal of Environmental Science International
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• v.28 no.2
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• pp.203-209
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• 2019

Organic wastewater generated from polyester manufacturing processes was selected from H company to investigate the feasibility of anaerobic digestion that produces gases including methane. Bio Methane Potential (BMP) tests were conducted to measure the gas production and methane concentration for 7 process wastewater and 2 kinds of sludges from the H company. Also, along with monitoring pH and alkalinity during the anaerobic digestion process, the concentrations of COD and 1,4-dioxane were measured with 4 different operating conditions for N Emulsion (NE) and Ethylene Glycol (EG) wastewater. The BMP tests showed that 65% of methane was produced from NE and EG wastewater. This suggests that the organic wastewater from H company can be effectively treated by an anaerobic digester by which more than 90% of COD was removed.

• Journal of Hydrogen and New Energy
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• v.30 no.2
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• pp.111-118
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• 2019

Greenhouse gas emissions have a profound effect on global warming. Various environmental regulations have been introduced to reduce the emissions. The largest amount of greenhouse gases, including carbon dioxide, is produced in the steel industry. To decrease carbon dioxide emission, hydrogen-based iron oxide reduction, which can replace carbon-based reduction has received a great attention. Iron production generates various by-product gases, such as cokes oven gas (COG), blast furnace gas (BFG), and Linz-Donawitz gas (LDG). In particular, COG, due to its high concentrations of hydrogen and methane, can be reformed to become a major source of hydrogen for reducing iron oxide. Nevertheless, continuous COG cannot be supplied under actual operation condition of steel industry. To solve this problem, this study proposed to use two alternative COG-based fuel mixtures; one with natural gas and the other with biogas. Reforming study on two types of mixed gas were carried out to evaluate catalyst performance under a variety of operating conditions. In addition, methane conversion and product composition were investigated both theoretically and experimentally.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1597-1605
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• 2014

Methane is considered as a next-generation carbon feedstock owing to the vast reserves of natural and shale gas. Methane can be converted to methanol by various methods, which in turn can be used as a starting chemical for the production of value-added chemicals using existing chemical conversion processes. Methane monooxygenase is the key enzyme that catalyzes the addition of oxygen to methane. Methanotrophic bacteria can transform methane to methanol by inhibiting methanol dehydrogenase. In this paper, we review the recent progress made on the biocatalytic conversion of methane to methanol as a key step for methane-based refinery systems and discuss future prospects for this technology.