• Title/Summary/Keyword: Mesh Pattern

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Plant Regeneration and Protein Analysis from Cadmium Resistant Callus of Tobacco (Nicotiana tabacum cv. BY4) (담배 (Nicotiana tabacum cv. BY4)카드뮴 저항성 캘러스로부터 식물체 재생과 단백질 분석)

  • 오승철;소웅영;조덕이;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.7-13
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    • 2001
  • Calli were induced from diploid and haploid tobacco after 4 weeks and maintained on MS medium with combination of 2.0 mg/L 2,4-D,0.1 mg/L BAP and 2.0 mg/L kinetin. Suspension cells were screened through 65 $\mu$m-nylon mesh and 100 $\mu$m-mesh, then they were smeared on selection medium combined with cadmium and PFP by using the low melting agarose of 0.8%. After 30days smeared cultures of the medium the cell was treated with 500 $\mu$M and 1000 $\mu$M to select the resistant cell line were selected. Plant regeneration was induced from the selected cell lines on medium with 0.5, 1.5, 2.0 mg/L BAP and on media with combination of auxin and BAP under 500 $\mu$M and 1000 $\mu$M cadmium. At this time, plant regeneration was achived on cadmium free medium. In case of haploid, occurred from the cell line which is selected in medium with cadmium and PFP. In case of diploid regeneration occurred is in the medium with cadmium alone. The plantlet regenerated from cadmium resistant calli grew well in cadmium 500 $\mu$M. Protein pattern of leaf, root, stem of regenerated plants was analyzed. The quantum was 6.5188 ug/mg.fr.wt in the leaf of plant, 5.3611 ug/mg.fr.wt in the stem, 3.0213 ug/mg.fr.wt in the root. On the other hand, 5.9652 ug/mg.fr.wt. in the leaf of control, 3.5974 ug/mg.fr.wt in the stem of the control, 4.3766 ug/mg.fr.wt. in the root of the control. The one dimension bends regenerated from cadmium resistant calli resistant to cadmium in leaf were 49 involving 198.7KD etc. Disappeared were 4 involving 160.5KD etc, The protein bends were combinized were 3 involving 83.4KD etc. The bends resistant to cadmium stress in stem were 41 involving 4.3KD etc. Disappeared were 5 involving 114.8KD etc. The protein bends combinized were 6 involving 128.7KD etc. The bends which had the resistance to cadmium stress in root is 27 in volving 166,9KD etc. The bends which disappeared were 198.7KD etc. There were 5 involving 83.4KD etc.

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Design and Evaluation of a Distributed Mutual Exclusion Algorithmfor Hypercube Multicomputers (하이퍼큐브 멀티컴퓨터를 위한 분산 상호배제 알고리즘의 설계 및 평가)

  • Ha, Sook-Jeong;Bae, Ihn-Han
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.9
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    • pp.2221-2234
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    • 1997
  • Distributed mutual exclusion algorithms have employed two approaches to achieve mutual exclusion and can be divided into two broad classes:token-based and permission-based. Token-based algorithms share a unique token among the nodes and a node is allowed to access its common resources if it possesses the token. Permission-based algorithms require one or more successive rounds of message exchanges among the nodes to obtain the permission to access the common resources. A hypercube architecture has earned wide acceptance in multiprocessor systems in the past few years because of its simple, yet rich topology. Accordingly, we study distributed permission-based mutual exclusion algorithms for hypercubes, and design a distributed permission-based mutual exclusion algorithm based on a new information structure adapted to the hypercubes. The new information structure is a request set of T-pattern from a logical mesh that is embedded into a hypercube. If a node wants to access the common resources, it sends request message to all nodes in the request set by Lan's multicast algorithm. Once the node receives a grant message from all nodes in the request set, it accesses the common resource. We evaluate our algorithm with respect to minimum round-trip delay, blocking delay, and the number of messages per access to the common resource.

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Effect of PVP(polyvinylpyrrolidone) on the Ag Nano Ink Property for Reverse Offset Printing (PVP(polyvinylpyrrolidone)가 리버스 오프셋용 은 나노 잉크 물성에 미치는 영향)

  • Han, Hyun-Suk;Kwak, Sun-Woo;Kim, Bong-Min;Lee, Taik-Min;Kim, Sang-Ho;Kim, In-Young
    • Korean Journal of Materials Research
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    • v.22 no.9
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    • pp.476-481
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    • 2012
  • Among the various roll-to-roll printing technologies such as gravure, gravure-offset, and reverse offset printing, reverse offset printing has the advantage of fine patterning, with less than 5 ${\mu}m$ line width. However, it involves complex processes, consisting of 1) the coating process, 2) the off process, 3) the patterning process, and 4) the set process of the ink. Each process demands various ink properties, including viscosity, surface tension, stickiness, and adhesion with substrate or clich$\acute{e}$; these properties are critical factors for the printing quality of fine patterning. In this study, Ag nano ink was developed for reverse offset printing and the effect of polyvinylpyrrolidone(PVP), used as a capping agent of Ag nano particles, on the printing quality was investigated. Ag nano particles with a diameter of ~60 nm were synthesized using the conventional polyol synthesis process. Ethanol and ethylene glycol monopropyl ether(EGPE) were used together as the main solvent in order to control the drying and absorption of the solvents during the printing process. The rheological behavior, especially ink adhesion and stickiness, was controlled with washing processes that have an effect on the offset process and that played a critical role in the fine patterning. The electrical and thermal behaviors were analyzed according to the content of PVP in the Ag ink. Finally, an Ag mesh pattern with a line width of 10 ${\mu}m$ was printed using reverse offset printing; this printing showed an electrical resistivity of 36 ${\mu}{\Omega}{\cdot}cm$ after sintering at $200^{\circ}C$.

Restoration planning of the Seoul Metropolitan area, Korea toward eco-city

  • Lee, Chang Seok
    • Proceedings of the Korea Society of Environmental Biology Conference
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    • 2003.06a
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    • pp.1-5
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    • 2003
  • In order to prepare a basis for ecological restoration of the Seoul Metropolitan area, ecological diagnoses on soil physico-chemical properties and vegetation structure were carried out. Land use patterns, actual vegetation, and biotope patterns were also investigated based on aerial photograph interpretation and field checks. I formulated landscape elements overlaying those data and evaluated the ecological value of each element. Soil pollution was evaluated by analyzing soil samples collected in each grid on the mesh map, divided by 2km $\times$ 2km intervals. Soil samples were collected in forests or grasslands escaped from direct human interference. Soil pollution evaluated from pH, and SO$_4$, Ca, Mg, and Al contents of soil was more severe in the urban outskirts than in the urban center. Those soil environmental factors showed significant correlation with each other. Vegetation in the urban area was different in species composition from that in suburban areas and showed lower diversity compared with that in the suburban areas. Successional process investigated by population structure of major species also showed a difference. That is, successional trend was normal in suburban areas, but that in urban areas showed a retrogressive pattern. The landscape ecological map of Seoul indicates that the urban center lacks vegetation and greenery space is restricted in urban outskirts. Such an uneven distribution of vegetation has caused a specific urban climate and thereby contributed to aggravation of air and soil pollution, furthermore causing vegetation decline. From this result, it was estimated that such uneven distribution of vegetation functioned as a trigger factor to deteriorate the urban environment. I suggested, therefore, a restoration plan based on landscape ecological principles, which emphasizes connectivity and even distribution of green areas throughout the whole area of the Seoul to solve this complex environmental problem. In this restoration plan, first of all, I decided the priority order for connection of the fragmented greenery spaces based on the distances from the core reserves comprised of green belt and rivers, which play roles as habitats of wildlife as well as for improvement of urban environment. Next, I prepared methods to restore each landscape element included in the paths of green network to be constructed in the future on the bases of such preferential order. Rivers and roads, which hold good connectivity, were chosen as elements to play important roles in constructing green network by linking the fragmented greenery spaces.

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Preliminary Analysis of the CANDU Moderator Thermal-Hydraulics using the CUPID Code (2상 유동 해석코드 CUPID를 이용한 CANDU 원자로 감속재 열수력 예비해석)

  • Park, Sang Gi;Lee, Jae Ryong;Yoon, Han Young;Kim, Hyoung Tae;Jeong, Jae Jun
    • Journal of Energy Engineering
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    • v.21 no.4
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    • pp.419-426
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    • 2012
  • A transient, three-dimensional, two-phase flow analysis code, CUPID, has been developed in KAERI. In this work, we performed a preliminary analysis using the CUPID code to investigate the thermal-hydraulic behavior of the moderator in the Calandria vessel of a CANDU reactor. At first, we validated the CUPID code using the three experiments that were performed at Stern Laboratories Inc. To avoid the complexity to generate computational mesh around the Calandria tube bundles, a porous media approach was applied for the region. The pressure drop in the porous media zone was modeled by an empirical correlation. The results of the calculations showed that the CUPID code can predict the mixed flow pattern of forced and natural convection inside the Calandria vessel very well. Thereafter, the analysis was extended to a two-phase flow condition. Also, the local maximum temperature in the Calandria vessel was plotted as a function of the injection flow rate, which may be utilized to predict the local subcooling margin.

Influence of Various Parameter for Nonlinear Finite Element Analysis of FRP-Concrete Composite Beam Using Concrete Damaged Plasticity Model (콘크리트 손상 소성모델을 이용한 FRP-콘크리트 합성보의 비선형 유한요소해석에서 여러 변수들의 영향)

  • Yoo, Seung-Woon;Kang, Ga-Ram
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.2
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    • pp.697-703
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    • 2017
  • This paper examines the flexure behavior of FRP-concrete composite structure that can replace conventional reinforced concrete structure types. In order to investigate the structural performance and behavioral characteristics in numerical analysis means, ABAQUS, a general purpose finite element analysis program, was utilized for nonlinear finite element analysis, and the various variables and their influences were analyzed and compared with experimental results to suggest values optimized to this composite structure. The concrete damage plasticity model and Euro code for concrete were used. In the implicit finite element analysis, the convergence was ambiguous when geometrical and material nonlinearity were large, so the explicit finite element analysis used in this study was deemed to be appropriate. From the comparison with the experiment about concrete damaged plasticity model, 20mm for the mesh size, $30^{\circ}$ for the dilation angle, $100Nmm/mm^2$ for the value of fracture energy, 0.667 for Kc value, and the consideration of damage parameter were suggested believed to be appropriate. The numerical model suggested in this study was able to imitate the ultimate load and cracking pattern very well; therefore, it is expected to be utilized in research of various new material composite structures.

Analysis of Wireless Network in Freight Container Terminal and Methods for Service Quality Enhancement (컨테이너 터미널의 무선 네트워크 분석과 서비스 품질 향상 방안)

  • Han, Seung-Ho;Park, Hyun-Sung;Kim, Jong-Deok;Kim, Yong-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.3B
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    • pp.235-246
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    • 2009
  • The number of industry wireless networks based on IEEE 802.11 WLAN technology deployed in large outdoor work sites, such as freight container terminal, is increasing these days. Wider service coverage, frequent movements of stations and high requirement on service reliability are main characteristics of these networks compared to the conventional IEEE 802.11 networks used in home, office and school. While the importance of these networks Bets higher, we can hardly find previous studies including concrete analysis on these networks based on actual experiments. We carried out several field experiments at a freight container terminal to analyze its IEEE 802.11 network and found out some problematic situations, such as over 50% retransmission rates and frequent disruption of communication link while a station is moving. We explain why these problematic situations happen and suggest some solutions, such as application of mesh technology, smart handoff based on location and movement pattern and adjustment of signal strength and channel allocation. Methods and tools used in the experiments are also detailed in the paper which may be helpful for similar future studies.

Development of Design Support Tool for Building 3D printing (건축물 3D 프린팅 설계지원도구 개발)

  • Lee, Dongyoun;Seo, Myoung-Bae;Ju, Ki-Beom
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.10
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    • pp.94-105
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    • 2020
  • Recently, most studies of 3D printing in construction have focused on the development of 3D printers and materials suitable for construction 3D printers. In comparison, there has been little research on design support tools that enable representative BIM data of building modeling tools to be applied to 3D printing. In addition, existing 3D printing slicing programs are commercialized around manufacturing, showing that they are unsuitable for construction 3D printing. Therefore, this research aims to develop a design support tool for 3D printing for buildings. The developed design support tool was validated based on arbitrary BIM data. Verification showed that wall pattern generation was modeled accurately without errors, and a calculation of the construction period showed that the formula presented in this study was valid. Furthermore, the maximum length of the mesh split was set to 100mm to minimize errors when converting to STL files.

Determination of 3-phenoxybenzoic Acid in Urine and Exposure Assessment of Pyrethroid Insecticides to Human Being (요중 3-phenoxybenzoic acid 미량 분석 및 pyrethroid계 살포자 노출 평가)

  • Seo, Jong-Chul;Song, Jae-Seok;Choi, Hong-Soon
    • The Korean Journal of Pesticide Science
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    • v.11 no.2
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    • pp.87-94
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    • 2007
  • Pyrethroid insecticide have widely been used for agricultural sector and residential environments. To assess the exposure of insecticide which is absorbed through skin the analysis of urinary metabolite is essential. At present, the urinary 3-PBA was analyzed using liquid-phase extraction. But LPE have many limitations, such as long pre-treatment time and low recovery. So, this study was conducted to determine the optimum conditions for analysing 3-PBA in urine using solid phase extraction. Furthermore, this study intend to investigate the relation of concentrations of pyrethroid, deltamethrin in air and 3-PBA in urine. The optimum condition for hydrolysis was found to be done with hydrochloric acid for one hour. The recovery rates of 3-PBA were $84.6%{\pm}1.2%$, $54.8{\pm}0.9%$, $99.8{\pm}1.2%$ with XAD-2, XAD-7, XAD-16 using as the aborbents and acetone as eluents respectively. But acetonitrle and methanol gave low recovery rate and methyl cellosolve could not elute the compound. The amount of acetone for elution were 6mL, 9mL, 3mL for XAD-2, XAD-7, XAD-16 as absorbents respectively. The non-absorbed rates was $0.8{\pm}0.5%$, and $0.7{\pm}0.3%$ under XAD-16, mesh size 140-200, amount of resin 1.4g and the flow rate of eluent was 0.1mL/min. In the concentration process, we obtained 11 times higher concentration of material. The amounts of urinary 3-PBA were. The LODs of 3-PBA and deltamethrin were 0.004 mg/L, 0.038 mg/L, respectively. The further research of minute monitoring which include spray pattern, environmental condition is needed And more research about the relation between total pyrethroid exposure and urinary various metabolite are also necessary.

CFD ANALYSIS OF TURBULENT JET BEHAVIOR INDUCED BY A STEAM JET DISCHARGED THROUGH A VERTICAL UPWARD SINGLE HOLE IN A SUBCOOLED WATER POOL

  • Kang, Hyung-Seok;Song, Chul-Hwa
    • Nuclear Engineering and Technology
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    • v.42 no.4
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    • pp.382-393
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    • 2010
  • Thermal mixing by steam jets in a pool is dominantly influenced by a turbulent water jet generated by the condensing steam jets, and the proper prediction of this turbulent jet behavior is critical for the pool mixing analysis. A turbulent jet flow induced by a steam jet discharged through a vertical upward single hole into a subcooled water pool was subjected to computational fluid dynamics (CFD) analysis. Based on the small-scale test data derived under a horizontal steam discharging condition, this analysis was performed to validate a CFD method of analysis previously developed for condensing jet-induced pool mixing phenomena. In previous validation work, the CFD results and the test data for a limited range of radial and axial directions were compared in terms of profiles of the turbulent jet velocity and temperature. Furthermore, the behavior of the turbulent jet induced by the steam jet through a horizontal single hole in a subcooled water pool failed to show the exact axisymmetric flow pattern with regards to an overall pool mixing, whereas the CFD analysis was done with an axisymmetric grid model. Therefore, another new small-scale test was conducted under a vertical upward steam discharging condition. The purpose of this test was to generate the velocity and temperature profiles of the turbulent jet by expanding the measurement ranges from the jet center to a location at about 5% of $U_m$ and 10 cm to 30 cm from the exit of the discharge nozzle. The results of the new CFD analysis show that the recommended CFD model of the high turbulent intensity of 40% for the turbulent jet and the fine mesh grid model can accurately predict the test results within an error rate of about 10%. In this work, the turbulent jet model, which is used to simply predict the temperature and velocity profiles along the axial and radial directions by means of the empirical correlations and Tollmien's theory was improved on the basis of the new test data. The results validate the CFD model of analysis. Furthermore, the turbulent jet model developed in this study can be used to analyze pool thermal mixing when an ellipsoidal steam jet is discharged under a high steam mass flux in a subcooled water pool.