• Title/Summary/Keyword: Membrane properties

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Research Progress on Strategies for Improving the Enzyme Properties of Bacteriophage Endolysins

  • Yulu Wang;Xue Wang;Xin Liu;Bokun Lin
    • Journal of Microbiology and Biotechnology
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    • v.34 no.6
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    • pp.1189-1196
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    • 2024
  • Bacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, some methods have been used to improve the enzyme properties (bactericidal activity, lysis spectrum, stability and targeting the substrate, etc) of bacteriophage endolysins, including deletion or addition of domains, DNA mutagenesis, chimerization of domains, fusion to the membrane-penetrating peptides, fusion with domains targeting outer membrane transport systems, encapsulation, the usage of outer membrane permeabilizers. In this review, research progress on the strategies for improving their enzyme properties are systematically presented, with a view to provide references for the development of lysins with excellent performances.

The Limiting Current Density and the Regeneration of a Heterogeneous Ion Exchange Membrane in a Nickel Plating Rinse Waters Treatment Process by Electrodialysis (전기투석에 의한 니켈도금 폐수처리 공정에서 한계전류밀도와 불균질 이온교환막의 재생)

  • 윤용수
    • Journal of environmental and Sanitary engineering
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    • v.16 no.2
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    • pp.38-46
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    • 2001
  • In this work, the heterogeneous ion exchange membrane was used in a electrodialysis apparatus to treat a Ni planting rinse water because the heterogeneous ion exchange membrane was excellent efficiency as compared with low manufacturing cost, was easy to make, and had a good mechanical properties. For a regeneration of membrane and to obtain the optimal condition for a scale-up of apparatus after treating Ni plating rinse water, we would find about the limiting current density and the concentration polarization. When the Ni plating rinse water 150mg/L was treated with the electrodialysis apparatus using the heterogeneous ion exchange membrane, the limiting current density was about $1.49{\;}mA/\textrm{cm}^2$. And the limiting current density increased with the flow rate and concentration of Ni plating rinse water. We recognized that the used membrane could be reused by periodic backwashing because efficiency was constant when the membrane was backwashed after treating wastewater.

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Nanofiltration of Dye Solutions Through Polyamide Composite Membranes

  • Jonggeon Jegal;Baek, Kyung-Sook;Lee, Kew-Ho
    • Korean Membrane Journal
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    • v.4 no.1
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    • pp.12-19
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    • 2002
  • Nanofiltration of aqueous dye solutions was carried out using polyamide (PA) nanofiltration (NF) composite membranes. The PA composite membranes were prepared by the interfacial polymerization of piperazine (PIP) and trimesoyl chloride (TMC) on the surface of microporous polysulfone (PSf) ultrafi1tration (UF) membranes. After characterization in terms of their permeation performance and surface ionic property, they were used for the separation of dye solutions such as Direct Red 75, 80, 81, and Direct Yellow 8 and 27. The separation conditions were varied to study the factors affecting on the permeation performance of the membranes: different concentrations of dye solutions, operating temperature and time, and flow rate of a feed solution. The surface property of the membrane, especially its ionic property, as a function of operating time was examined with a zeta-potentiometer and the relationship between the surface chemistry of the membrane and its permeation properties was also studied.

Ultrafiltration of Oily Wastewater with Surface Pretreated Membranes

  • Kim, Kyu-Jin;Fane, Antony G.
    • Korean Membrane Journal
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    • v.1 no.1
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    • pp.43-49
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    • 1999
  • Separation of soluble oil was investigated during filtration of cutting oil emulsion using various commercial ultrafiltration membranes. The surface properties of membranes used were hydrophilic hydrophobic and modified surfaces by various surfactant pretreatments. Conditions varied include stirring speed transmeembrane pressure membrane type and surfactant type for pretreatment. The results give some indication of mechanisms occurring at the membrane surface. Surfactant pretreatments significantly improved water flux and UF flux of hydrophilic regenerated cellulose(up to 2.4x for YM100) and hydrophobic polysulfone (up to 2.2x for PTHK) membranes depending on surfactant type and operating conditions. The UF flux enhancement was attributed to membrane swelling and reduction of interfacial surface tension between oil droplets and membrane surface. unexpectedly the hydrophilic membranes revealed greater flux enhancement than the hydrophobic membranes. The results also showed a greater improvement in UF flux at lower operating pressure.

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Synthetic membranes in Biotechnology: Realities and Possibilities

  • Belfort, Georges
    • Proceedings of the Membrane Society of Korea Conference
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    • 1992.10a
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    • pp.15-33
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    • 1992
  • Synthetic membrane processes are being increasingly integrated into existing reaction, isolation, and recovery schemes for the production of valuable biological molecules. In many cases they are replacing traditional unit processes. The properties of membrane systems which are most often exploited for both upstream and downstream processing are their permselectivity, high surface area per unit volume, and their potential for controlling the level of contact and/or mixing between two separate phases. Advances in both membrane materials and module design and operation have led to better control of concentration polarization and membrane fouling. After presenting some recent advances in membrane materials and fluid mechanics, we demonstrate how membranes have been integrated into cellular and enzymatic reaction schemes. This is followed by a review of established and emerging membrane separation processes. Several examples are used to emphasize the synergism between biological processes and synthetic membranes.

A Pilot Plant Study of Industrial Wastewater Recycling Technology for Disc-Tube Membrane (DISC-TUBE MEMBRANE을 이용한 산업폐수 재활용 기술의 PILOT PLANT적 연구)

  • 김동일;한성욱;김호식;김인환
    • Journal of environmental and Sanitary engineering
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    • v.12 no.3
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    • pp.81-86
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    • 1997
  • In case of Industrial Wastewater, It was various pollutions, high concentration and different physical, chemical properties each other in accordance with classification of wastewater. Therefore, after inquiring into the influence on the membrane of the dissolved pollutants, we should select the membrane of best efficient quality. As results of experiments on pilot plant test, optimum operating pressure for fouling removal was 34BAR, when continues operating was 34 BAR, recovery rate was 75% and permeate water flux was $32.9{\;}{\ell}/hr{\cdot}m^{2}$.

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Submerged Membrane Breakwaters I: A Rahmen Type System Composed of Horizontal and Vertical Membranes

  • Kee, Sung-Tae
    • International Journal of Ocean Engineering and Technology Speciallssue:Selected Papers
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    • v.5 no.1
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    • pp.14-21
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    • 2002
  • In the present paper, the hydrodynamic properties of a Rahmen-type, flexible, porous breakwater interacting with obliquely or normal- incident small amplitude waves are numerically investigated. This system is composed of dual vertical porous membranes, hinged at the side edges of a submerged horizontal membrane. The dual vertical membranes are extended downward and hinged at seabed. The effects of permeability, Rahmen-type membrane breakwater geometry, pre-tensions on membranes, relative dimensionless wave number, and incident wave headings are thoroughly examined.

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Modification of ultrafiltration membranes with carbon nanotube buckypaper for fouling alleviation

  • Guo, Jin;Liu, Jian-Hong;Wang, Li-Ying;Liu, Hong
    • Membrane and Water Treatment
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    • v.6 no.1
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    • pp.1-13
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    • 2015
  • The modification of ultrafiltration membranes with carbon nanotube (CNT) buckypaper on fouling control was investigated. Two types of commercially available flat-sheet membranes were used: PS35 and PES900C/D (PES) (the PS35 membranes were hydrophilic with a molecular weight cutoff of 20 kDa, and the PES membranes were hydrophobic with a molecular weight cutoff of 20 kDa). The CNT buckypaper modified ultrafiltration membranes were prepared by filtering a CNT suspension through the flat-sheet membrane in a dead-end ultrafiltration unit. After modification, the pure water flux of PES was significantly increased, while the pure water flux of PS35 was decreased. The properties of the CNT modified membranes were also investigated. Considering the antifouling properties, pure water flux of the modified membrane, and the stability of CNT buckypaper layer on the membrane surface, ethanol solution with a concentration of 50 wt.%, multi-walled carbon nanotubes (MWCNTs) with a larger diameter (30-50 nm), and the CNT loading with $7.5g/m^2$ was selected. The CNT buckypaper on the surface of ultrafiltration membranes can trap the pollutants in sewage effluent and prevent them reaching the surface of virgin membranes. Water quality analysis showed that the effluent quality of the modified membrane was obviously improved. The removal efficiency of humic acid and protein-like matters by the modified membrane was significant. These results indicate the potential application of the CNT buckypaper layer modified membranes in the field of wastewater reclaim.

Formation of Poly(vinylidene difluoride) Membranes with Various Pore Sizes by a Phase Inversion Process and Membrane Performance of Aqueous and Non-aqueous Solution System (상전환법에 의한 다양한 기공크기를 갖는 폴리비닐리덴플루오라이드 막의 제조와 수계 및 비수계 용액 내에서의 막 성능)

  • Lee Kew-Ho;Kim In-Chul
    • Membrane Journal
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    • v.15 no.1
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    • pp.22-33
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    • 2005
  • Asymmetric PVDF membranes were prepared by the phase inversion from casting solutions containing PVDF, NMP as solvent and 1,4-dioxane, DGDE, acetone, or GBL as additives by immersing them in water. The effects of various additives on the casting solution properties, permeation properties, and membrane structures were investigated. Low miscibility of 1,4-dioxane, DGDE and acetone with the coagulant (water) compared with NMP resulted in reducing the membrane pore size. When DGDE is used as an additive, the pore size was reduced because of its incipient sharp interface formation in the water. GBL increased membrane pore size because of its polarity compared to that of NMP. The PVDF membranes with various pore sizes could be obtained by controlling the amount of additive. The effect of mixed solvent (aqueous and non-aqueous solution) on permeation through membrane was investigated. Not only solution viscosity but surface tension affected solvent permeation.