• 에너지공학
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• 제13권2호
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• pp.93-100
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• 2004

간접법을 이용한 표면오염도 측정 시 시료채취와 동시에 방사선 검출이 가능한 스메어 매체를 제조하고 성능을 평가하였다. 스메어 매체는 무기섬광체를 고분자 막에 함침시킨 것으로서, 용매로는 디메틸포름아마이드(DMF)와 메틸렌클로라이드(MC) 그리고 고분자 소재로서 폴리설폰(PSF)을 사용하였고, 무기섬광체는 CAYS(cerium activated yttrium silicate)를 사용하였다. 함침막은 단일 및 이중구조로 제조하였고, 물. 메타놀 등 비용매성 욕조에 침지하거나 용매 증발을 통하여 고형화하여 특성을 비교하였다. 방사선과의 상호작용에 의하여 무기섬광물질 함침막으로부터 방출되는 광자의 계측은 광전증배관과 고전압원, 증폭기. 계수기로 구성된 원형계수기를 사용하였다. $^{14}$ C표면오염에 대해 무기섬광 함침막을 이용해 얻은 계수율을 통상의 저준위 알파/베타 계수기로 얻은 결과와 비교할 때 상대 감도가 약 50%로 나타났다. 또한, 계수효율이 상대적으로 낮았지만 저에너지 베타선 방출핵종인 $^3$H표면오염도측정이 가능하다는 것을 확인하였다.

• Archives of Pharmacal Research
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• 제28권6호
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• pp.699-708
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• 2005

The present study was designed to investigate the effect of naloxone, a well known opioid antagonist, on the secretion of catecholamines (CA) evoked by cholinergic stimulation and membrane-depolarization in the isolated perfused rat adrenal glands, and to establish its mechanism of action. Naloxone ($10^{-6}\~10^{-5}$ M), perfused into an adrenal vein for 60 min, produced dose- and time-dependent inhibition of CA secretory responses evoked by ACh ($5.32\times10^{-3}$ M), high K+ ($5.6\times10^{-2}$ M), DMPP ($10^{-4}$ M) and McN-A-343 ($10^{-4}$ M). Naloxone itself also failed to affect the basal CA output. In adrenal glands loaded with naloxone ($3\times10^{-6}$ M), the CA secretory responses evoked by Bay-K-8644, an activator of L-type $Ca^{2+}$ channels, and cyclopiazonic acid, an inhibitor of cytoplasmic $Ca^{2+}$-ATPase, were also inhibited. In the presence of met-enkephalin ($5\times10^{-6}$ M), a well known opioid agonist, the CA secretory responses evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were also significantly inhibited. Taken together, these results suggest that naloxone greatly inhibits the CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors as well as that by membrane depolarization. It seems that these inhibitory effects of naloxone does not involve opioid receptors, but might be mediated by blocking both the calcium influx into the rat adrenal medullary chromaffin cells and the uptake of $Ca^{2+}$ into the cytoplasmic calcium store, which are at least partly relevant to the direct interaction with the nicotinic receptor itself.

• KSBB Journal
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• 제16권6호
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• pp.533-537
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• 2001

유청 단백질 중에서 $\alpha$-lactalbumin, $\beta$-lactoglobulin를 고성능 막 크로마코그래피를 이용하여 분리하는 것이다. 분리 메카니즘은 음이온 교환작용이며 고정상은 CIM DEAE, QA, So$_3$ disk (16$\times$3 mm)을 사용하였다. 이동상은 buffer A (20 mM Tris-HCI, pH 7.3)와 buffer B (buffer A + 1 M NaCl)를 사용하였으며 $\alpha$-lactalbumin, $\beta$-lactoglobulin를 분리하기 위해서 구배용매 조성법을 사용하였다. 각 이동상의 조성에 따른 최적의 이동상 조성(Buffer A/Buffer B=100/0 - 30/70 vol%, gradient time 1 min, 30/70 - 10/90 vol%, gradient time 2 min)을 실험적으로 얻었고 4 ml/min의 이동상 유속에서 3분내에 $\alpha$-lactalbumin, $\beta$-lactoglobulin를 분리 할 수 있었다. 유청 단백질 중에 $\alpha$-lactalbumin, $\beta$-lactoglobulin을 HPMC을 적용하여 분리하였고, 유청 단백질의 기능적 성질, 분리 방법에 대해 알아보았다.

• Natural Product Sciences
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• 제13권1호
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• pp.68-77
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• 2007

The aim of the present study was to examine the effects of green tea extract (CUMS6335) on the release of CA evoked by cholinergic stimulation and direct membrane-depolarization in the perfused model of the adrenal gland isolated from the spontaneously hypertensive rats (SHRs), and to establish the mechanism of action. Furthermore, it was also to test whether there is species difference between animals, and between CUMS6335 and EGCG, one of biologically the most powerful catechin compounds found in green tea. CUMS6335 $(100\;{\mu}g/ml)$, when perfused into an adrenal vein for 60 min, time-dependently inhibited the CA secretory responses evoked by ACh (5.32mM), high $K^+$(56 mM), DMPP $(100\;{\mu}M)$, and McN-A-343 $(100\;{\mu}M)$ from the isolated perfused adrenal glands of SHRs. However, CUMS6335 itself did fail to affect basal catecholamine output. Also, in adrenal glands loaded with CUMS6335 $(100\;{\mu}g/ml)$, the CA secretory responses evoked by Bay-K-8644 $(10\;{\mu}M)$ and cyclopiazonic acid $(10\;{\mu}M)$ were also inhibited in a relatively time-dependent fashion. However, in the Presence of EGCG $(8.0\;{\mu}g/ml)$ for 60 min, the CA secretory response evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were not affected except for last period. Collectively, these results indicate that CUMS6335 inhibits the CA secretion evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors as well as by direct membrane-depolarization from the perfused adrenal gland of the SHR. It seems that this inhibitory effect of CUMS6335 is exerted by blocking both the calcium influx into the rat adrenal medullary chromaffin cells and the uptake of $Ca^{2+}$ into the cytoplasmic calcium store, which are at least partly relevant to the direct interaction with the nicotinic receptor itself. It seems likely that there is much difference in mode of the CA-releasing action between CUMS6335 and EGCG.

• Journal of Dental Anesthesia and Pain Medicine
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• 제18권4호
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• pp.223-233
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• 2018

Topical anesthetics are commonly used in oral & maxillofacial surgery to control pain in the oral cavity mucosa before local anesthetic injection. These anesthetic agents come in many forms, developed for different usages, to minimize adverse reactions, and for optimal anesthetic efficiency. Earlier studies have revealed that these agents may also limit the growth of microorganisms in the area of anesthetic application. Many topical anesthetic agents show different levels of antimicrobial activity against various bacterial strains and Candida. The dosage of local anesthetic agent used in some clinical preparations is too low to show a significant effect on microbial activity. Efficiency of antimicrobial activity depends on the local anesthetic agent's properties of diffusion within the bloodstream and binding efficiency with cytoplasmic membrane, which is followed by disruption of the bacterial cell membrane. The antimicrobial properties of these agents may extend their usage in patients to both control pain and infection. To develop the topical local anesthetic optimal usage and antimicrobial effect, a collaborating antiseptic agent may be used to benefit the local anesthetic. However, more research is required regarding minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of topical local anesthetic agents with drug interaction between anesthetics and antiseptic agents.

• 대한의생명과학회지
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• 제9권3호
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• pp.159-165
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Recently, the studies with C-terminus of ankyrins have identified that ankyrin-B is capable of interacting with Hsp40 and sAnkl is capable of interacting with obscurin and titin, but the function of C-terminal domain of ankyrin-G remains unknown. To identify proteins interacting C-terminus of ankyrin-G, we used the C-terminus of ankyrin-G as a bait for a yeast two-hybrid screen of brain cDNA library. Approximately 1.33$\times$l0$^6$ transformants were screened, of which 13 positive clones were obtained as determined by activation of HIS3, ADE2 and MELl reporter genes. Sequence analyses of these 13 plasmids revealed that cDNA inserts of 13 colonies showed highly homologous to 11 genes, including 5 known (i.e., Na$^+$/K$^+$ ATPase $\beta$1, SERBPl, UTF2, cytochrome C oxidase and collagen IV $\alpha$2) and 6 unknown genes. The evaluation of the proteins that emerge from these experiments provides a rational approach to investigate the those proteins significant in interaction with ankyrin-G.

• Reproductive and Developmental Biology
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• 제36권1호
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• pp.55-64
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• 2012

Peroxiredoxin II (Prdx II; a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Prdx II has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in a variety of disease states associated with oxidative stress, including cancer and aging-associated pathologies. But, the precise mechanism is still obscure in various aspects of aging containing ovarian aging. Identification and relative quantification of the increased proteins affected by Prdx II deficiency may help identify novel signaling mechanisms that are important for oxidative stress-related diseases. To identify the increased proteins in Prdx $II^{-/-}$ mice, we performed RBC comparative proteome analysis in membrane fraction and cytosolic fractions by nano-UPLC-$MS^E$ shotgun proteomics. We found the increased 86 proteins in membrane (32 proteins) and cytosolic (54 proteins) fractions, and analyzed comparative expression pattern in healthy RBCs of Prdx $II^{+/+}$ mice, healthy RBCs of Prdx $II^{-/-}$ mice, and abnormal RBCs of Prdx $II^{-/-}$ mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cellular morphology and assembly, cell-cell interaction, metabolism, and stress-induced signaling. Moreover, protein networks among the increased proteins were analyzed to associate with various diseases. Taken together, RBC proteome may provide clues to understand the clue about redox-imbalanced diseases.

• 폴리머
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• 제28권6호
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• pp.502-508
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• 2004

암을 치료하기 위한 양이온성 리포솜은 화학치료요법의 분야에서 발전되어 왔다. 양이온성 리포솜은 음이온성의 세포 표면에 정전기적 상호작용에 의해 결합이 된다. 본 연구의 목적은 음이온성 세포와 이온결합을 할 수 있는 양이온성 리포솜을 제조하는 것이다. 양이온성 리포솜은 지질인 1,2-디스테로일-sn-글리콜-3-포스포에탄올아민 (DSPE)과 양이온성 고분자인 폴리에틸렌이민 (PEI)을 그라프트 중합으로 합성된 물질로부터 제조하였다. 리포솜 표면의 이온 특성은 제타 포텐셜을 측정하여 확인하였다. 소 혈청 수용액에서 리포솜의 혈장 단백질 흡착 특성은 입자 크기와 탁도 변화를 측정하여 확인하였다. 완충 용액 속에서 리포솜 안정성을 예측하기 위하여 입자 크기를 7일 동안 상온에서 측정하였다. 양이온성 리포솜은 소 혈청 수용액에서 많은 양의 혈장 단백질이 흡착되었다. 이는 혈장 단백은 음전하를 가지고 있어서 양이온성 리포솜의 표면과 잘 붙기 때문이다. 양이온성 폴리에틸렌이민을 이용한 리포솜의 표면 변형은 소 혈청 수용액 내에서 단백질 흡착을 강화시킨다는 것을 예상하게 한다. 또한, 제조된 리포솜의 완충 용액 내에서 7일 동안 안정한 상태임을 관찰하였다.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2011년도 정기 학술대회
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• pp.531-538
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• 2011

멤브레인형 LNG 탱크의 구조적 안전성을 확보하기 위해서는 슬로싱에 의한 작용 압력과 구조응답을 정확히 평가할 수 있어야 한다. 탱크 방열구조에 작용하는 슬로싱 충격하중은 매우 불규칙적이며 이로 인한 구조 응답 역시 유탄성 거동을 포함하는 매우 복잡한 물리 현상이기 때문에 최신의 이론적 실험적 접근 방법을 동원하더라도 정확한 평가가 어렵다. 본 연구에서는 실험이나 수치해석으로부터 얻어진 슬로싱 압력 시계열을 이용하여 탱크 방열 구조의 구조응답을 간편하게 해석할 수 있는 방안을 제안하였다. 이 간이 해석법은 기본적인 삼각형 impulse 형태의 충격 압력에 대한 구조응답을 시간영역에서 과도응답해석법으로 계산한 후, 이렇게 구해진 구조물의 삼각형 응답함수를 조합하여 임의 형상의 압력 시계열에 대한 구조 응답을 구하는 방식이다. 여러 가지 예제 해석을 통하여 제안된 해석법의 타당성을 검토하였고, 이를 이용하여 실제 모형실험에서 얻어진 압력시계열을 바탕으로 구조응답을 계산하고 그 결과를 고찰하였다.

• Bulletin of the Korean Chemical Society
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• 제28권9호
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• pp.1499-1509
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• 2007

Proteomic analyses of synaptic vesicle fraction from rat brain have been performed for the better understanding of vesicle regulation and signal transmission. Two different approaches were applied to identify proteins in synaptic vesicle fraction. First, the isolated synaptic vesicle proteins were treated with trypsin, and the resulting peptides were analyzed using a high-pressure capillary reversed phase liquid chromatography/tandem mass spectrometry (cRPLC/MS/MS). Alternatively, proteins were separated by two-dimensional gel electrophoresis (2DE) and identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS). Total 18 and 52 proteins were identified from cRPLC/MS-MS and 2DE-MALDI-TOF-MS analysis, respectively. Among them only 2 proteins were identified by both methods. Of the proteins identified, 70% were soluble proteins and 30% were membrane proteins. They were categorized by their functions in vesicle trafficking and biogenesis, energy metabolism, signal transduction, transport and unknown functions. Among them, 27 proteins were not previously reported as synaptic proteins. The cellular functions of unknown proteins were estimated from the analysis of domain structure, expression profile and predicted interaction partners.