• Wind and Structures
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• 제19권1호
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• pp.35-58
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• 2014

A numerical simultaneous solution involving a linear elastic model was applied to study the fluid-structure interaction (FSI) of membrane structures under wind actions, i.e., formulating the fluid-structure system with a single equation system and solving it simultaneously. The linear elastic model was applied to managing the data transfer at the fluid and structure interface. The monolithic equation of the FSI system was formulated by means of variational forms of equations for the fluid, structure and linear elastic model, and was solved by the Newton-Raphson method. Computation procedures of the proposed simultaneous solution are presented. It was applied to computation of flow around an elastic cylinder and a typical FSI problem to verify the validity and accuracy of the method. Then fluid-structure interaction analyses of a saddle membrane structure under wind actions for three typical cases were performed with the method. Wind pressure, wind-induced responses, displacement power spectra, aerodynamic damping and added mass of the membrane structure were computed and analyzed.

• Bulletin of the Korean Chemical Society
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• 제17권3호
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• pp.279-284
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• 1996

Decay of the spin label attached to cytochrome c or to stearic acid has been measured by electron paramagnetic resonance (EPR) spectroscopy to monitor membrane oxidation induced by cytochrome c-membrane interaction. Binding of cytochrome c sequestered the acidic phospholipids and membrane oxidation was efficient in the order linoleic oleic>stearic acid for a fatty acid chain in the acidic phospholipids. The spin label on cyt c was destroyed at pH 7 whereas that on stearic acid embedded in the membrane was destroyed at pH 4, presumably due to different modes of cyt c-membrane interaction depending on pH. Interestingly, cyt c also interacts with phosphatidylethanolamine, an electrically neutral phospholipid, to cause rapid membrane oxidation. Both EPR and fluorescence measurements indicated that electrostatic interaction is at least partially responsible for the process.

• Structural Engineering and Mechanics
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• 제31권3호
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• pp.349-365
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• 2009

Membrane structures are quite sensitive to wind and therefore the fluid-solid interaction can not be neglected in dynamic analysis. A boundary element method (BEM) for 3D simulation of wind-structure interaction in tensile membrane structures is presented in this paper. The flow is treated as incompressible and potential. The flow field is solved with boundary element method codes and structural simulation is performed by finite element method software ANSYS. The nonlinear equations system is solved iteratively, with segregated treatment of the fluid and structure equations. Furthermore this method has been demonstrated to be effective by typical examples. Besides, the influence of several parameters on the wind-structure interaction, such as rise-span ratio, prestress and the wind velocity are investigated according to this method. The results provide experience in wind resistant researches and engineering.

• 멤브레인
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• 제31권2호
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• pp.145-152
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• 2021

현재 바이오 분야에서 분리에 대한 수요가 급증함에 따라, 투과율 및 결합능 측면에서 높은 성능을 띠는 막크로마토그래피가 수지 크로마토그래피의 대체 분리 공정으로 부상하고 있다. 실증을 기반으로 하여 막 소재가 결정되는 기존 분리막 공정과 달리, 막크로마토그래피의 경우 분리하고자 하는 목표 물질에 적합한 분리 메커니즘 이해 그리고 이를 기반한 공정 설계가 필요하다. 본 논문에서는 생특이성을 활용하여 선택적으로 거대 분자를 포집하는 친화성 작용, 전하를 활용하여 생분자와 결합하는 이온 교환 작용 그리고 소수성을 활용하여 생분자와 결합하는 소수성 작용과 같은 막크로마토그래피 주요 분리 메커니즘들에 대해 다루고자 한다. 또한 본 논문에서는 단계적 측면에서 또는 소재 측면에 막크로마토그래피 기술설계 시 고려해야할 변인들에 대해서 다루고자 한다.

• Wind and Structures
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• 제22권3호
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• pp.349-368
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• 2016

A preconditioning technique is presented for a simultaneous solution to wind-membrane interaction. In the simultaneous equations, a linear elastic model was employed to deal with the fluid-structure data transfer at the interface. A Lagrange multiplier was introduced to impose the specified boundary conditions at the interface and strongly coupled simultaneous equations are derived after space and time discretization. An initial linear elastic model preconditioner and modified one were derived by treating the linearized elastic model equation as a saddle point problem, respectively. Accordingly, initial and modified fluid-structure interaction (FSI) preconditioner for the simultaneous equations were derived based on the initial and modified linear elastic model preconditioners, respectively. Wind-membrane interaction analysis by the proposed preconditioners, for two and three dimensional membranous structures respectively, was performed. Comparison was made between the performance of initial and modified preconditioners by comparing parameters such as iteration numbers, relative residuals and convergence in FSI computation. The results show that the proposed preconditioning technique greatly improves calculation accuracy and efficiency. The priority of the modified FSI preconditioner is verified. The proposed preconditioning technique provides an efficient solution procedure and paves the way for practical application of simultaneous solution for wind-structure interaction computation.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2001년도 추계학술대회
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• pp.97-101
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• 2001

The fusion between viral envelope and target cell membrane is a central step of viral infection, and the fusion proteins located at viral envelope mediate such process. Gp41 of HIV is one of the fusion proteins whose structure and mechanism of membrane fusion had been extensively studied. Functionally important motives of gp41 are the N-terminus fusion peptide, the coiled-coil and the membrane proximal C-peptide regions. The role of these regions during the fusion process had been thoroughly examined. Specially, insertion of the fusion peptide into membrane and conformational change of the coiled-coil and C-peptide regions are assumed to be critical for the fusion mechanism. In addition, the coiled-coil region has been shown to interact with membrane, and the C-peptide region regulates the interaction in a dose dependent manner. Furthermore, fusion defective mutations of the coiled-coil region dramatically changed its binding affinity to membrane. These results suggested that the membrane binding property of the coiled-coil region is important for the fusion activity of gp41, and such property could be modulated by the interaction with the C-peptide region.

• Structural Engineering and Mechanics
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• 제37권4호
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• pp.401-413
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• 2011

The aerodynamic stability of orthotropic tensioned membrane structures with rectangular plane is theoretically studied under the uniform ideal potential flow. The aerodynamic force acting on the membrane surface is determined by the potential flow theory in fluid mechanics and the thin airfoil theory in aerodynamics. Then, based on the large amplitude theory and the D'Alembert's principle, the interaction governing equation of wind-structure is established. Under the circumstances of single mode response, the Bubnov-Galerkin approximate method is applied to transform the complicated interaction equation into a system of second order nonlinear differential equation with constant coefficients. Through judging the stability of the system characteristic equation, the critical divergence instability wind velocity is determined. Finally, from different parametric analysis, we can conclude that it has positive significance to consider the characteristics of orthotropic and large amplitude for preventing the instability destruction of structures.

• 대한의생명과학회지
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• 제17권2호
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• pp.167-171
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• 2011

[ ${\alpha}$ ]synuclein (${\alpha}$-syn) is implicated in the pathogenesis of Parkinson's disease (PD) and other related diseases. We have previously reported that ${\alpha}$-syn binds to the cell membranes in a transient and reversible manner. However, little is known about the physiologic function and/or consequence of this association. Here, we examined whether chemically induced perturbations to the cellular membranes enhance the binding of ${\alpha}$-syn, based on hypothesis that ${\alpha}$-syn may play a role in maintenance of membrane integrity or repair. We induced membrane perturbations or alterations in ${\alpha}$-syn-overexpressing human neuroblastoma cells (SH-SY5Y) by treating the cells with hydrogen peroxide ($H_2O_2$) or oleic acid. In addition, membranes fractionated from these cells were perturbed by treating them with proteinase K or chloroform. Dynamic interaction of ${\alpha}$-syn to the membranes was analyzed by the chemical cross-linking assay that we developed in the previous study. We found that membrane interaction of ${\alpha}$-syn was increased upon treatment with membrane-perturbing reagents in a dose and time dependent manner. These results suggest that perturbations in the cellular membranes cause increased binding of ${\alpha}$-syn, and this may have significant implication in the physiological function of ${\alpha}$-syn in cells.

• 한국해양공학회지
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• 제15권2호
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• pp.11-21
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• 2001

The focus of this paper is on the numerical investigation of obliquely incident wav interactions with a system composed of fully submerged and floating dual buoy/vertical-flexible-membrane breakwaters placed in parallel with spacing between two systems. The fully submerged two systems allow surface and bottom gaps to enable wave transmission over and under the system. The problem is formulated based on the two-dimensional multi-domain hydro-elastic linear wave-body interaction theory. The hydrodynamic interaction of oblique incident waves with the combination of the rigid and flexible bodies was solved by the distribution of the simple sources (modified Bessel function of the second kind) that satisfy the Helmholz governing equation in fluid domains. A boundary element program for three fluid domains based on a discrete membrane dynamic model and simple source distribution method is developed. Using this developed computer program, the performance of various dual systems varying buoy radiuses and drafts, membrane lengths, gaps, spacing, mooring-lines stiffness, mooring types, water depth, and wave characteristics is thoroughly examined. It is found that the fully submerged and floating dual buoy/membrane breakwaters can, if it is properly tuned to the coming waves, have good performances in reflecting the obliquely incident waves over a wide range of wave frequency and headings.

• Bulletin of the Korean Chemical Society
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• 제35권11호
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• pp.3389-3392
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• 2014