• 생약학회지
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• 제25권3호
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• pp.272-277
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• 1994

Meliae toosendan Fructus, the seed of Pagoda tree, Melia toosendan Sieb. et Zucc. (Meliaceae), has been used as a liver protective herbal drug in the Orient. Administration of seed oil to hyperlipedemic rats revealed the lipid lowering effects in serum and liver tissue without any toxicity in body weight increase and liver function.

• 식물분류학회지
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• 제45권1호
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• pp.36-44
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• 2015

한약재 천련자로 유통되는 중국 식물과 우리나라에도 분포하는 멀구슬나무의 연관성을 규명하기 위하여 중국남서부지역에서 천련자(Melia toosendan)로 채취하는 재료와 중국 남동부, 한국, 인도 등지에서 심고 있는 멀구슬나무(M. azadarach), 그리고 무환자나무과의 근연종들을 대상으로 핵 ITS, 엽록체 matK, rbcL, atpF-H, psbK-I, psbA-trnH 등 6개 마커의 염기서열을 비교분석하였다. 이들 염기서열 자료를 계통분석한 결과 한약재 천련자로 알려진 식물과 멀구슬나무는 동일종으로 판단된다. 이 두 종은 학자들에 따라서는 동일종 또는 이종으로 처리하던 것으로, 본 연구자들의 유전자 비교분석결과는 동일종으로 취급하는 것이 보다 타당함을 지지한다. 또한, 다양한 이명으로 세계 각지에서 기재된 멀구슬나무에 가장 가까운 종은 약재로 잘 알려진 님나무(Azadirachta indica)로, 이 종은 이전에 멀구슬나무속으로도 처리된 바 있다.

• 생약학회지
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• 제24권1호
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• pp.63-68
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• 1993

Meliae toosendan Fructus is the fruit of Melia toosendan $S_{IEB}$. et $Z_{UCC}$. (Meliaceae), which is written in oriental terminology as clearing heat and drying dampness, and also explained using liver, stomach and small intestine for channels entered. Among the five fractions prepared from methanol whole extractive of the herb, the chloroform fraction which suggests the presence of triterpenoid, flavonoid and alkaloid stimulated the activities of drug metabolizing enzymes and bile secretion and lowered the serum transaminase activities of liver damaged by carbon tetrachloride.

• Natural Product Sciences
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• 제29권1호
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• pp.17-23
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• 2023

A phytochemical investigation of the fruits extract of Melia toosendan afforded the isolation of two new nor-neolignans, meliasendanins E (1) and F (2), as well as twelve known compounds (3 - 14) using various separation technique such as Diaion HP20, silica, RP-18 gel column chromatography and semi-preparative HPLC. Their chemical structures were elucidated by extensive NMR spectroscopic data including 2D-NMR, and HR-ESI-MS as well as ECD data. Among the twelve known compounds, the absolute structures of 3 - 6 were determined first, and given the trivial names as meliasendanins G-J (3 - 6). Based on the evaluation of anti-inflammatory activity, compounds 7 - 8 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC₅₀ values of 34.6 and 39.5 µM, respectively.

• 생약학회지
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• 제27권1호
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• pp.47-52
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• 1996

A triterpenoid(melianone) and a limonoid(28-deacetyl sendanin) were isolated from the chloroform fraction of Meliae toosendan Fructus, the rippen fruits of Melia toosendan SIEB. et ZUCC.(Meliaceae) and were applied to serial experiments to clarify the liver protective activities. We found that the compounds promoted slightly the drug metabolizing enzyme activities and decreased serum transaminase activities which was elevated by carbon tetrachloride intoxication. And also they slightly increased the secretion of bile juice in rat.

• 약학회지
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• 제38권1호
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• pp.6-11
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• 1994

The MeOH extract of the fruits of Melia toosendan was selected for futher study by its cytotoxicity and effect on the human breast cancer cell line, MCF-7. The active principle obtained by activity guided fractionation followed by purification gave rise to a needle crystal. The structure was deduced by employing NMR and was determined to be identical with 28-deacetyl sendanin by comparison with published data. This compound induced morphological change of MCF-7 to be rounded with tubule at concentrations between $50\;{\mu}g/ml$ and $0.025\;{\mu}g/ml$. This compound, however, showed strong cytotoxic effect on Hepalclc7 and HepG2, and their $GI_{50}$ on the hepatoma cell lines were $0.238\;{\mu}g/ml$ and $0.805\;{\mu}g/ml$, respectively. Its effect on lymphocyte of mouse was stronger than hepatoma cell lines, and their $ED_{50}$ of polyclonal antibody response was $0.011\;{\mu}g/ml$, and $ED_{50}$ of cell viability was $0.039\;{\mu}g/ml$.

• 대한본초학회지
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• 제23권4호
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• pp.121-134
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• 2008

Objectives: Melia toosendan(MT) has been used as a traditional Korean herbal medicine, and today it is used as a medication for colic, side aches, heartache and other disorders of liver. The aim of this study was to determine whether fractionated extracts of MT inhibit free radical generation such as DPPH radical, superoxide radical and nitric oxide, production of nitrite, an index of NO, PGE2, iNOS, COX-2 and proinflammatory cytokines in lipopolysaccharide(LPS)-treated RAW 264.7 macrophages. Methods: MT extract prepared with methanol, and then fractionated with hexane, dichloromethane, ethylacetate, n-butanol and water. Inhibitory effect of MT onto free radical generation was determined by measuring DPPH, superoxide anions and nitric oxide scavenging activities in vitro. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt(MTS) assay. Intracelluar oxidation was analysed by DCF-DA assay. The nitric oxide(NO) production was measured by Griess reagent system. The levels of iNOS and COX-2 expression were confirmed by western blot. And pro inflammatory cytokines were measured by ELISA kit. Results: Our results indicated that fractionated extracts, especially dichloromethane and ethyl acetate extracts, significantly inhibited free radical generation, the LPS-induced $H_2O_2$, NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of TNF-${\alpha}$, IL-1${\beta}$ and IL-6 formation in macrophages. Conclusions: These results indicate that dichloromethane and ethyl acetate extracts of MT have potential as an agent of chronic inflammatory diseases.

• Archives of Pharmacal Research
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• 제17권2호
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• pp.100-103
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• 1994

The limonoid compound (28-deacetyl sendanin0 isolated from the fruit of Melia toosendan SIEB. et ZUCC. was evaluated on anticancer activity. According to a standard in vitro cytotoxicity assy, eight human cancer cell lines and SRB assay were introduced for present evaluation. As a positive standard, adriamycin was tested in parallel. The cell lines were originated from six different organs. In view of dose-response profiles to 28-deacetyl sendanin, the most sensitive cells were SF-539 and PC-3 which were derived from CNS and prostate, respecitively. In contrast, all the cell lines responded similarly to adriamycin to give rise to nearly indentical six cell lines were more sensitive to 28-deacetyl sendanin and two were more resistant. As a result, 28-deacetyl sendanin had more senstive and selective inhibitory effects on in vitro growth of human cancer cell lines in a comparison with adriamycin.

• 생약학회지
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• 제28권4호
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• pp.174-178
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• 1997

Methanol extracts from 450 plants were screened for muttidrug-resistance reversing activity using drug sensitive KB-3-1 and multidrug-resistant KB-Vl cells. Among them, the extracts of Cynanchum wilfordii, Torilis japonica, Celastrus orbiculatus, Melia toosendan and Teminialia chebula strongly potentiated vinblastine cytotoxicity in KB-Vl cells. But their cytotoxicities to both sensitive KB-3-1 and resistant KB-Vl cells were in the same order of magnitude. These results indicate that the above samples would contain the active principles which do not exert their ativity solely by cytotoxicity.

• 혜화의학회지
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• 제4권1호
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• pp.445-448
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• 1995

Although significant progress has been made during the past four decades in the treatment of human neoplastic disease, effective chemotherapeutic treatments are nevertheless far from satisfactory to patients suffering from cancer, particularly to those with the stomach cancer which is most prevalent in Korea. To find out the effective anticancer drugs, especially human stomach cancer, we tested cytotoxic activities of several medicinal herbs against human stomach cancer cell line, SNU-1. Evodia officinalis, Melia toosendan, Sinomenium acutum, Galla Haeepensis, Celastrus orbiculatus and Taxus caespitosa showed a significant cytotoxic activities.