• Title/Summary/Keyword: Medium capacity

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Electron Transport Activities of Chloroplasts Isolated from the Detached Rice Leaves Stored under Low Temperature with Illumination (광 및 저온처리한 벼잎 절편에서 분이한 엽록체의 전자전달 활성)

  • 문병용
    • Journal of Plant Biology
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    • v.31 no.4
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    • pp.299-307
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    • 1988
  • The electron transport activities of choloroplasts isolated from hte detached rise (Oryza sativa L. cv. Chucheong) leave stored under low temperature(4$^{\circ}C$) with light illumination were investigated to understand the role of light in the low temperature inhibition of photosynthesis in the chilling-sensitive plants. Chlorophyll content of the detached leaves upon incubation at 28$^{\circ}C$ and 4$^{\circ}C$ in the dark was also measured. The rice seedlings were grown with Hoagland medium in the growth chamber of 28$^{\circ}C$ temperature and 400 ft.c fluorescent light with the photoperiod of 16 h. Although chlorophyll content of the detached leaves stored in the dark declined by 61.7% after 28$^{\circ}C$ treatement, there occurred only 5.2% decrease of chlorophyll with 4$^{\circ}C$ treatment. Low temperature treatment(4$^{\circ}C$) for 6 days brought about decreases in total photosystem(PS II+PS I) activities by 35.2% and 73.6% in te presence and absence of light, respectively, while after 28$^{\circ}C$ treatment of the detached leaves for 6 days in the dark there was only 27.6% decrease in PS II+PS I activity. PS II activities were also decreased by 35.6% and 72.2% in the light and dark, respectively. PS I activities were decreased slightly, however, by 7.6% and 16.2% in the light and dark, respectively. Investigations into DPClongrightarrowDCPIP and NH2OHlongrightarrowDCPIP activities revealed that low temperature inhibition of PS II activities was not due to the inactivation of the water oxidation capacity at low temperature. It was concluded that light protects the electron transport activities of isolated rice chloroplasts from the inhibitory effect of low temperature in the detached leaves.

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Flip Chip Assembly Using Anisotropic Conductive Adhesives with Enhanced Thermal Conductivity

  • Yim, Myung-Jin;Kim, Hyoung-Joon;Paik, Kyung-Wook
    • Journal of the Microelectronics and Packaging Society
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    • v.12 no.1 s.34
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    • pp.9-16
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    • 2005
  • This paper presents the development of new anisotropic conductive adhesives with enhanced thermal conductivity for the wide use of adhesive flip chip technology with improved reliability under high current density condition. The continuing downscaling of structural profiles and increase in inter-connection density in flip chip packaging using ACAs has given rise to reliability problem under high current density. In detail, as the bump size is reduced, the current density through bump is also increased. This increased current density also causes new failure mechanism such as interface degradation due to inter-metallic compound formation and adhesive swelling due to high current stressing, especially in high current density interconnection, in which high junction temperature enhances such failure mechanism. Therefore, it is necessary for the ACA to become thermal transfer medium to improve the lifetime of ACA flip chip joint under high current stressing condition. We developed thermally conductive ACA of 0.63 W/m$\cdot$K thermal conductivity using the formulation incorporating $5 {\mu}m$ Ni and $0.2{\mu}m$ SiC-filled epoxy-bated binder system to achieve acceptable viscosity, curing property, and other thermo-mechanical properties such as low CTE and high modulus. The current carrying capability of ACA flip chip joints was improved up to 6.7 A by use of thermally conductive ACA compared to conventional ACA. Electrical reliability of thermally conductive ACA flip chip joint under current stressing condition was also improved showing stable electrical conductivity of flip chip joints. The high current carrying capability and improved electrical reliability of thermally conductive ACA flip chip joint under current stressing test is mainly due to the effective heat dissipation by thermally conductive adhesive around Au stud bumps/ACA/PCB pads structure.

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Seismic Capacity Evaluation of Low-Rise Reinforced Concrete Buildings in Korea (국내 저층 철근콘크리트 건물의 내진성능 평가)

  • Lee, Kang-Seok;Kim, Yong-In;Min, Kyung-Min
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.11a
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    • pp.241-244
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    • 2008
  • The authors proposed a new rapid-screening method for more reasonably evaluating seismic capacities of medium and low-rise RC buildings controlled by both shear and flexure in Ref. [1]. The method proposed in Ref. [1] was based on relationships between required strengths of each failure system for ductility factors and damage degrees of overall system derived from the view-point of ductility factors. The proposed method was also verified using observed real damage data of low-rise RC buildings caused by past earthquakes. Results indicated that the methodology proposed in Ref. [1] compares well with real damages and is a useful strategy for rapidly identifying low-rise RC buildings having high potential seismic risk. In this study, in order to verify the applicability of the new methodology proposed in Ref. [1] to real RC building systems, seismic capacities of existing eleven low-rise RC buildings in Korea are evaluated based on the new method.

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Effects of Several Herbal Medicines on Alkaline Phosphatase Activity in Human Fetal Osteoblasts (수종의 생약제제가 human fetal osteoblasts의 염기성 인산분해 효소 활성에 미치는 영향)

  • Lee, Myoung-Ku;Choi, Hee-In;You, Hyung-Keun;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.33 no.1
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    • pp.49-60
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    • 2003
  • Several growth factors and polypeptides are not commonly yet used for regenerators of bone tissue or alveolar bone because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many herbal medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, antiinflammatory and regenerative potential of periodontal tissues. Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have been traditionally used as medicines for treatment of bone disease in Eastern medicine. The objective of the present study is to examine the ability of alkaline phosphatase (ALP) activity of human fetal osteoblast (hFOB1) when several natural medicines were supplemented. hFOB1 were cultured with Dulbecuo's Modified Eagle's Medium Nutrient Mixture F-12 HAM ( DMEM/F-12 1:1 Mixture, Sigma, USA) and negative control, dexamethasone (positive control), and each natural medicines for 3 days. And then ALP activity was measured by spectrophotometer for enzyme activity and Alizarin red S staining for morphometry. Among the natural medicines of this study, Morindae Radix, Cibotium Barometz (L.) and Cistanchis Herba induced higher activity of ALP synthesis than negative controls in all experimental group. Albizziae Cortex showed mild increases than negative control group. According to measurement of positively stained area, all of the natural medicines of this study increased compared to negative control. Especially, Cibotium Barometz (L.) and Cistanchis Herba showed statistical significance compared to negative control (p<0.05). These results indicate that Morindae Radix, Cibotium Barometz (L.), Albizziae Cortex, Cistandhis Herba have an inducing ability of ALP synthesis on osteoblast.

DNA Information Hiding Method for DNA Data Storage (DNA 데이터 저장을 위한 DNA 정보 은닉 기법)

  • Lee, Suk-Hwan;Kwon, Ki-Ryong
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.10
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    • pp.118-127
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    • 2014
  • DNA data storage refers to any technique for storing massive digital data in base sequence of DNA and has been recognized as the future storage medium recently. This paper presents an information hiding method for DNA data storage that the massive data is hidden in non-coding strand based on DNA steganography. Our method maps the encrypted data to the data base sequence using the numerical mapping table and then hides it in the non-coding strand using the key that consists of the seed and sector length. Therefore, our method can preserve the protein, extract the hidden data without the knowledge of host DNA sequence, and detect the position of mutation error. Experimental results verify that our method has more high data capacity than conventional methods and also detects the positions of mutation errors by the parity bases.

A Study on Detection of Small Export Companies Utilizing Trade Exports Live Index (무역수출 라이브지수를 활용한 중소수출기업 발굴 연구)

  • Kim, Heecheon;Leem, Choon Seong;Sung, Juwon
    • The Journal of Bigdata
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    • v.4 no.2
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    • pp.115-126
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    • 2019
  • There have been many discussions on export indices in trade exports, but there is no definite trade export index which can be explained by objective indicators. Korea International Trade Association (KITA), Korea Trade-Investment Promotion Agency (KOTRA), etc., but we are currently in the process of thinking about ways to express the capabilities of exporting companies. In this study, we constructed the AI data sets by setting the activity indicators such as the size of the company and the credit score, the number of transaction customers, the number of transactions, the number of items, the transaction volume, and the transaction period as features, Lightgbm. Using the Graph Neural Network as an industrial cluster classification model, the export live index which expresses the exportable capacity among companies, items, and business groups was calculated. This includes the past activity of the company from the current calculating index Objectivity.

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Influence of the amount of tooth surface preparation on the shear bond strength of zirconia cantilever single-retainer resin-bonded fixed partial denture

  • Sillam, Charles-Ellie;Cetik, Sibel;Ha, Thai Hoang;Atash, Ramin
    • The Journal of Advanced Prosthodontics
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    • v.10 no.4
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    • pp.286-290
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    • 2018
  • PURPOSE. Conventional resin-bonded fixed partial dentures (RBFPDs) are usually made with a two-retainer design. Unlike conventional RBFPDs, cantilever resin-bonded fixed partial dentures (Cantilever RBFPDs) are, for their part, made with a single-retainer design. The aim of this study was to compare the effect of tooth surface preparation on the bond strength of zirconia cantilever single-retainer RBFPDs. The objective is to evaluate the shear bond strength of these single-retainer RBFPDs bonded on 3 different amount of tooth surface preparation. MATERIALS AND METHODS. Thirty extracted bovine incisors were categorized to 3 groups (n=10), with different amounts of tooth surface preparations. Teeth were restored with single-retainer RBFPDs with different retainer surfaces: large retainer of $32mm^2$; medium retainer of $22mm^2$; no retainer and only a proximal connecting box of $12mm^2$. All RBFPDs were made of zirconia and were bonded using an adhesive system without adhesive capacity. Shear forces were applied to these restorations until debonding. RESULTS. Mean shear bond strength values for the groups I, II, and II were $2.39{\pm}0.53MPa$, $3.13{\pm}0.69MPa$, and $5.40{\pm}0.96MPa$, respectively. Statistical analyses were performed using a one-way ANOVA test with Bonferroni post-hoc test, at a significance level of 0.001. Failure modes were observed and showed a 100% adhesive fracture. CONCLUSION. It can be concluded that the preparation of large tooth surface preparation might be irrelevant. For zirconia single-retainer RBFPD, only the preparation of a proximal connecting box seems to be a reliable and minimally invasive approach. The differences are statistically significant.

Behavior of stiffened and unstiffened CFT under concentric loading, An experimental study

  • Deifalla, Ahmed F.;Fattouh, Fattouh M.;Fawzy, Mona M.;Hussein, Ibrahim S.
    • Steel and Composite Structures
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    • v.33 no.6
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    • pp.793-803
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    • 2019
  • Concrete-filled steel tubular (CFST) beam-columns are widely used owing to their good performance. They have high strength, ductility, large energy absorption capacity and low costs. Externally stiffened CFST beam-columns are not used widely due to insufficient design equations that consider all parameters affecting their behavior. Therefore, effect of various parameters (global, local slenderness ratio and adding hoop stiffeners) on the behavior of CFST columns is studied. An experimental study that includes twenty seven specimens is conducted to determine the effect of those parameters. Load capacities, vertical deflections, vertical strains and horizontal strains are all recorded for every specimen. Ratio between outer diameter (D) of pipes and thickness (t) is chosen to avoid local buckling according to different limits set by codes for the maximum D/t ratio. The study includes two loading methods on composite sections: steel only and steel with concrete. The case of loading on steel only, occurs in the connection zone, while the other load case occurs in steel beam connecting externally with the steel column wall. Two failure mechanisms of CFST columns are observed: yielding and global buckling. At early loading stages, steel wall in composite specimens dilated more than concrete so no full bond was achieved which weakened strength and stiffness of specimens. Adding stiffeners to the specimens increases the ultimate load by up to 25% due to redistribution of stresses between stiffener and steel column wall. Finally, design equations previously prepared are verified and found to be only applicable for medium and long columns.

Investigation on the monotonic behavior of the steel rack upright-beam column connection

  • Cao, Yan;Alyousef, Rayed;Jermsittiparsert, Kittisak;Ho, Lanh Si;Alaskar, Abdulaziz;Alabduljabbar, Hisham;Alrshoudi, Fahed;Mohamed, Abdeliazim Mustafa
    • Smart Structures and Systems
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    • v.26 no.1
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    • pp.103-115
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    • 2020
  • The cold-formed steel storage racks are extensively employed in various industries applications such as storing products in reliable places and storehouses before distribution to the market. Racking systems lose their stability under lateral loads, such as seismic actions due to the slenderness of elements and low ductility. This justifies a need for more investigation on methods to improve their behavior and increase their capacity to survive medium to severe loads. A standardized connection could be obtained through investigation on the moment resistance, value of original rotational stiffness, ductility, and failure mode of the connection. A total of six monotonic tests were carried out to determine the behavior of the connection of straight 2.0 mm, and 2.6 mm thickness connects to 5 lug end connectors. Then, the obtained results are benched mark as the original data. Furthermore, an extreme learning machine (ELM) technique has been employed to verify and predict both moment and rotation results. Out of 4 connections, increase the ultimate moment resistance of connection by 13% and 18% for 2.0 mm and 2.6 mm upright connection, respectively.

STUDIES ON PRODUCTION AND EFFICIENT UTILIZATION OF LIVESTOCK EMBRYOS BY IN VITRO FERTILIZATION AND MICROMANIPULATION IV. NUCLEAR TRANSPLANTATION AND ELECTROFUSION FOR CLONING IN BOVINE FOLLICULAR OOCYTES

  • Chung, Y.C.;Kim, C.K.;Song, X.X.;Yoon, J.T.;Choi, S.H.;Chung, Y.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.6
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    • pp.641-645
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    • 1995
  • This study was conducted to develop a method for production of nuclear transplant bovine embryos using in vitro-matured (IVM) oocytes and to examine the effect of different conditions of electrofusion on fusion rate and developmental capacity of donor nucleus transplanted to enucleated oocytes. Eight- to sixteen-cell embryos derived from oocytes matured and fertilized in vitro used as donor blastomeres and IVM oocytes were used as recipient oocytes. Oocytes were enucleated immediately after 23-24 h IVM and then reconstituted with a donor blastomere in two different micromanipulation media. Fusion rate and subsequent development of the reconstituted oocytes was compared under the different electric stimuli and recipient oocyte ages. Success rate of enucleation was significantly higher in TCM-199 medium containing FCS than in DPBS. The high fusion rate(75-94%) and development (6.4-14.8%) to morulae and blastocyst (M + B) were obtained from 0.6-0.75 kV/cm DC voltage, although total cleavage was not different among the electric pulses. Most optimal condition of electric stimulation for fusion and development was 1 DC voltage of 0.75 kV/cm, in which 80.5% of oocytes were fused, 80.0% and 31.7% of which was cleaved and developed to M + B, respectively. No M + B was obtained from 1.2 kV/cm DC voltage regardless of pulse frequency. Recipint oocyte age at electrofusion greatly affected the cleavage and subsequent development to M + B, showing high rate at 40-41 h oocyte maturation. These results suggest that a suitable condition of electrofusion for donor nuclei derived from IVF may be 1-2 DC pulses of 0.7 kV/cm for $70{\mu}sec$ and that processing of a transplanted nucleus in IVM oocytes may be affected by maturation age of recipient oocytes.