• Title/Summary/Keyword: Medium capacity

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Plant Regeneration and Somatic Embryo Formation from Root-Derived Callus of Rice (벼 뿌리조직 유래의 캘러스로부터 체세포배 형성과 식물체 재분화)

  • 손재근;김경민;김종수
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.143-148
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    • 1995
  • The competence of callus formation and plant regeneration from root derived callus was higher in japonica cultivars than those of Tongil-type cultivars of rice. A japonica type cultivars Yeongdeogbyeo, showed the highest capacity (13%) for plant regeneration from root calli of 6 cultivars tested. The callus induced from seed and root tissues maintained higher capacity for plant regeneration during 7 passages of subculture on N$_{6}$ solid media at 2-week intervals. The maximum frequency (2 x 10$^{5}$ mL) of round cells and their cell colonies showed about 24 days after suspension culture of root-derived callus in N$_{6}$ medium with lmg/L 2,4-D, 300mg/L casein hydrolysate, 10mM L-proline, 20g/L sucrose and 30g/L sorbitol. The frequency of somatic embryo formation in suspension cultures of root-derived callus increased with prolonged advance of subculture time from 30 to 90 days, but their regenerative capacities decreased.

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Development of Connection Details for a Double Split Tee Connection Without a Shear Tab (전단탭이 없는 상·하부 스플릿 티 접합부의 접합부상세 개발)

  • Yang, Jae Guen;Kim, Yong Boem
    • Journal of Korean Society of Steel Construction
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    • v.28 no.1
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    • pp.53-64
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    • 2016
  • The double split tee connection, a type of beam-to-column moment connection, exhibits different behavioral characteristics according to changes in the thickness of the T-stub flange, the gauge distance of the high-strength bolt, and the number and diameter of high-strength bolts. In general, the double split tee connection is idealized and designed so that a T-stub fastened to the top and bottom supports a flexural moment, and a shear tab supports a shear force. However, if the double split tee connection is applied to low-and medium-rise steel structures, the size of the beam member becomes small, and thus the shear tab cannot be bolted to the web of a beam. In this regard, this study was conducted to propose connection details to ensure that the double split tee connection with a geometric shape can display sufficient shear resisting capacity. To this end, experiments were conducted using full-scale specimens for the double split tee connection.

Performance Analysis of Co- and Cross-tier Device-to-Device Communication Underlaying Macro-small Cell Wireless Networks

  • Li, Tong;Xiao, Zhu;Georges, Hassana Maigary;Luo, Zhinian;Wang, Dong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.4
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    • pp.1481-1500
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    • 2016
  • Device-to-Device (D2D) communication underlaying macro-small cell networks, as one of the promising technologies in the era of 5G, is able to improve spectral efficiency and increase system capacity. In this paper, we model the cross- and co-tier D2D communications in two-tier macro-small cell networks. To avoid the complicated interference for cross-tier D2D, we propose a mode selection scheme with a dedicated resource sharing strategy. For co-tier D2D, we formulate a joint optimization problem of power control and resource reuse with the aim of maximizing the overall outage capacity. To solve this non-convex optimization problem, we devise a heuristic algorithm to obtain a suboptimal solution and reduce the computational complexity. System-level simulations demonstrate the effectiveness of the proposed method, which can provide enhanced system performance and guarantee the quality-of-service (QoS) of all devices in two-tier macro-small cell networks. In addition, our study reveals the high potential of introducing cross- and co-tier D2D in small cell networks: i) cross-tier D2D obtains better performance at low and medium small cell densities than co-tier D2D, and ii) co-tier D2D achieves a steady performance improvement with the increase of small cell density.

Changes in Phosphate Transporter Activity Evaluated by Phosphonoformic Acid Binding in Cadmium-Exposed Renal Brush-Border Membranes

  • Chung, Jin-Mo;Ahn, Do-Whan;Kim, Kyoung-Ryong;Park, Yang-Saeng
    • The Korean Journal of Physiology and Pharmacology
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    • v.3 no.5
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    • pp.513-519
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    • 1999
  • Direct exposure of renal tubular brush-border membranes (BBM) to free cadmium (Cd) causes a reduction in phosphate (Pi) transport capacity. Biochemical mechanism of this reduction was investigated in the present study. Renal proximal tubular brush-border membrane vesicles (BBMV) were isolated from rabbit kidney outer cortex by Mg precipitation method. Vesicles were exposed to $50{\sim}200\;{\mu}M\;CdCl_2$ for 30 min, then the phosphate transporter activity was determined. The range of Cd concentration employed in this study was comparable to that of the unbound Cd documented in renal cortical tissues of Cd-exposed animals at the time of onset of renal dysfunction. The rate of sodium-dependent phosphate transport $(Na^+-Pi\;cotransport)$ by BBMV was determined by $^{32}P-Iabeled$ inorganic phosphate uptake, and the number of $Na^+-Pi$ cotransporters in the BBM was assessed by Pi-protectable $^{14}C-labeled$ phosphonoformic acid $([^{14}C]PFA)$ binding. The exposure of BBMV to Cd decreased the $Na^+-Pi$ cotransport activity in proportion to the Cd concentration in the preincubation medium, but it showed no apparent effect on the Pi-protectable PFA binding. These results indicate that an interaction of renal BBM with free Cd induces a reduction in $Na^+-Pi$ cotransport activity without altering the carrier density in the membrane. This, in turn, suggest that the suppression of phosphate transport capacity $(V_{max})$ observed in Cd-treated renal BBM is due to a reduction in $Na^+-Pi$ translocation by existing carriers, possibly by Cd-induced fall in membrane fluidity.

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THE EFFECT OF HYALURONIC ACID ON MOUSE CALVARIA PRE-OSTEOBLASTS OSTEOGENESIS IN VITRO (히알루론산이 골 형성에 미치는 영향에 관한 실험적 연구)

  • Cho, Yong-Min;Min, Seung-Ki;Kim, Soo-Nam;You, Yong-Ouk
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.3
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    • pp.216-225
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    • 2002
  • Hyaluronic acid (HA) is an almost essential component of extracellular matrices. Early in embryogenesis mesenchymal cells migrate, proliferate and differentiate, in part, because of the influence of HA. Since the features of embryogenesis are revisited during wound repair, including bone fracture repair, this study was initiated to evaluate whether HA has an effect on calcification and bone formation in an in vitro system of osteogenesis. Mouse calvaria Pre-osteoblast (MC3T3-E1) cells were cultured in ${\alpha}-MEM$ medium with microorganism-derivative hyaluronic acid that was produced by Strep. zooepidemicus which of molecular weight was 3 million units. The dosages were categorized in each 0.5, 1.0 and 2.0 mg/ml concentration experimental groups. After 2 and 4 days cultures in expeirmental and control groups, the tendency of cell proliferation, MTT assay, protein synthesis ability, collagen synthesis and alkaline phosphatase activity were analysed and bone nodule formation capacity were measured with Alizarin Red S stain after 29 days cultures. The cell proliferation was increased in time, especially the group of 0.5 and 1.0 mg/ml concentration of HA were showed prominent cell proliferation. After 2 and 4 days culture, experimental groups in general were greater cell activity in MTT assay. The protein synthesis was increased in all experimental groups compared to control group, especially most prominent in 1.0 mg/ml concentration group. The collagen synthesis capacity were increased in HA experimental groups, especially prominent in 1.0 mg/ml group and the activity of alkaline phosphatase were increased, especially also prominent in 1.0 mg/ml group, compared to control group. Above these, the activity of mouse carvarial pre-osteoblast cells was showed greater bone osteogenesis activity in all applied HA experimental group, especially group of 1.0 mg/ml concentration of HA.

A Study on Preference Attribute of Smart Learning for SMEs Work-Place Learning Innovation (중소기업의 직무교육 혁신을 위한 스마트러닝 선호 속성에 관한 연구)

  • Lee, Jung-Hwan;Chang, Hyun-Joon;Han, Yeong-Do
    • Journal of Korea Technology Innovation Society
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    • v.14 no.3
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    • pp.647-663
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    • 2011
  • Company's interest in the work place training and investment has been growing continuously because the talent of human resource is the competitiveness itself in the knowledge based society. However, corporate training programs mainly have focused on large companies and SMEs despite the economic business volume have been treated too lightly so far. This paper regards corporate training programs with one of the methods for SMEs innovation and proposes the smart learning in the smart device diffusion. Concretely, this paper analyzes the utilization intention, each attribute and level in smart learning characteristics using conjoint analysis. The result shows that SMEs have positive response for smart learning acceptance and SMEs consider significantly the usage fee type and location with the difference between regular employee and administrator. Specially, interactive communication and customized contents are preferred in the training type. Smart learning can be used as strategic means in supporting the value innovation and enhancing the absorptive capacity in SMEs innovation process.

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The Utilization of Ice at Home in Seoul and Kyunggi-Do Area (서울 및 경기지역 가정에서의 얼음 사용 실태에 관한 연구)

  • 장정옥;이영미
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.3
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    • pp.355-363
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    • 1997
  • This study was designed to investigate development points for automatic ice maker which met consumer's demand. Basic data about using ices in Korean's dietary life, were collected from Seoul and Kyunggi-do residences and analyzed by SPSS program. The results were as follows; 93.7% of respondents had 3 major domestic brands' refrigerator and 41.4% of them had medium-large capacity(400-519L). 65% of respondents had a refrigerator that was purchased less than five years. 15.5% of respondents had one more refrigerator. Among of them, major refrigerator was located in kitchen(67.7%) and minor was in kitchen(29.8%) and in veranda or multi-purpose room(26.3%). 66.2% of respondents always prepared ices in ice container, and 85% of them used ice only in summer. Boiling water with barley or corn was used as drinking water (45.6%) and boiled or purified tap water was used to make ice (38.6%) commonly. In cooking, ice was used especially in cold soup with cucumber and seaweed(89.9%), ice-tea or ice-coffee(81%), and fruit punch(64%). The next commonly using ice was adding in alcoholic beverage such as whiskey(57.4%), in Koreans traditional drink(Misugaru) (45.2%) and juice. The purpose of using ice was to cool in soft drink (58.6%), to cool in cooking food(19.9%), to eat ice itself(14.0%), and to enjoy more fantastic taste and mood(3.8%). In whiskey, the purpose of using ices was to control alcoholic content (52.9%). There was no significant difference between sex in the purpose of using ice, but there was significant differences between age(p<0.05). Seasonal variations in using ice, there were significant differences according to sex, age, roles in family, amount of preparation of ice, socio-economic level and brand and capacity of refrigerator in respondent's home.

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A Study on the Effect of the Information System Factors and the Organizational Factors of Venture Firms on Procedural Management Performance (벤처기업의 정보시스템 특성과 조직특성이 과정적 경영성과에 미치는 영향)

  • Kim, Gi-Jung;Yi, Seon-Gyu
    • Journal of Convergence for Information Technology
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    • v.8 no.2
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    • pp.209-218
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    • 2018
  • The purpose of this study is to analyze the influence of information system factors and organizational factors on procedural management performance in small and medium-sized venture companies in the Seoul metropolitan area. In the previous study was conducted mainly on large companies and general SMEs. In this study, the research was conducted considering the characteristics of SMEs. The results showed that system suitability, system quality, and IT assets had a positive effect on procedural management performance, and manager perception and human resource capacity had a positive effect on procedural management performance. But the evaluation and compensation did not have a positive effect on procedural management performance. The results of this study showed almost the same results as those of the previous studies. Venture firms have poor financial ability, technology, and organizational management ability than general SMEs. However, the introduction and diffusion factors of new information systems were not significantly different from those of general SMEs.

Advanced Formulation and Pharmacological Activity of Hydrogel of the Titrated Extract of C. Asiatica

  • Hong Soon-Sun;Kim Jong-Ho;Li Hong;Shim Chang-Koo
    • Archives of Pharmacal Research
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    • v.28 no.4
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    • pp.502-508
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    • 2005
  • Titrated extract of Centella asiatica (TECA) contains three principal ingredients, asiaticoside (AS), asiatic acid (AA), and madecassic acid (MA). These components are known to be clinically effective on systemic scleroderma, abnormal scar formation, and keloids. However, one problem associated with administration of TECA is its low solubility in aqueous as well as oil medium. In this study, various nonionic surfactants and bile salts as anionic surfactant were tested and screened for solubilizing TECA with a view to developing topical hydrogel type of ointment which is stable physicochemically, and has better pharmacological effects. When TECA was incorporated into various nonionic surfactant systems, labrasol had the most potent capacity for solubilizing TECA. In cases of bile salt systems, Na-deoxycholate (Na-DOC) had foremost solubilizing capacity, even more than labrasol. In differential scanning calorimetric study, the peaks of AA, MA, AS and Na-DOC disappeared at the coprecipitate of $1\%$ TECA and $1\%$ Na-DOC, suggesting the optimum condition of Na-DOC for solubilizing TECA. When the physicochemical stability of hydrogel containing this mixture was assessed, it was stable at room temperature for at least one month. Pharmacologically it significantly decreased the size of wound area at the $9^{th}$ day when applied to the wound area of rat dorsal skin. Taken together, solubility of TECA was dramatically improved by using non ionic and anionic surfactant systems, and Na-DOC was found to be the most effective solubilizer of TECA in formulating a TECA-containing hydrogel typed ointment. Moreover this gel was considered to be applicable to clinical use for wound healing effect.

Some Considerations on the Capacity Estimation of Emergency Electric Generator in Apartment Complex (공동주택의 비상발전기 용량산정방식에 관한 고찰)

  • Kim, Jeong-Tae;Rhee, Wook
    • The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.10 no.4
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    • pp.68-77
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    • 1996
  • In this study, for the capacity estimation of emergency electric generators built in apartment complex using NEGA C 201 method, it was proposed application methods of simultaneously starting groups and demand factors. Also, it was suggested that capacities of the electric generator might be calculated for two kinds of electric facilities; one is loads for the convenience of tenants when main electric supply cuts off, and the other is loads for fire. For 7 apartment complex of large-size (about 1, 500 households), medium- size (about 1, 000 households) and small - size (about 500 households), electric generator capacities were calculated and electric power were measured. Through the comparison between calculated values and measured values, it could be proved the suitability of these application methods. In addition, the calculated electric generator capacities were appeared to fit into engin capacities by NEGA C 201 method as well as those by conventional method.

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