• Journal of Microbiology and Biotechnology
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• v.7 no.3
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• pp.204-208
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• 1997

Aureobasidium puliulans possesses the capacity for simultaneous formation of fructosyltransferase and glucosyltransferase in various sugar media including sucrose, maltose, glucose and fructose. Among them, sucrose (300 g/1) was the most suitable carbon source for fructosyltransferase production, while fructose (100 g/1) gave the maximal production of glucosyltransferase. There existed a critical concentration for the optimal formation of enzymes in sucrose, glucose and fructose media. By contrast, no effect of maltose concentrations up to 300 g/1 was observed. The specific activity of the glucosyltransferase on maltose medium was highest during the early period of fetmentation, after which a sharp decrease occurred, whereas fructosyltransferase activity on sucrose medium maintained a nearly constant rate for a given culture period. Concomitant production of fructosyltransferase and glucosyltransferase was investigated with different combinations of lower concentrations of sucrose and maltose. Maltose supplementation in sucrose media and sucrose addition to maltose media enhanced the activity ratios of fructosyltransferase to glucosyltransferase as compared to that of non-supplemented media. Several polymers and surfactants were added in an attempt to enhance enzyme production, and supplementation of polyoxyethylene-sorbitan monolaurate (Tween 20) promoted fructosyltransferase production by 20%.

• Journal of Ocean Engineering and Technology
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• v.22 no.4
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• pp.78-83
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• 2008

In recent years, small boats used for marine leisure have been steadily increasing because of the increase in national income and the desire for marine leisure. But the repair of such small boats in dry dock has pointed out many faults in small FRP-shipbuilding in terms if workspace and manpower. Lifting a boat from the water to land is done with a crane or by hand using a sling around the bottom of the boat. But dry dock work is limited by the scale of the boat, which corresponds to the crane capacity, with carelessness making it possible to capsize a boat and endanger life. The purpose of this study was the development of a marine docking system that would improve economical efficiency and safety, for which we carried out concept design, model tests, structural analysis, etc.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.1
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• pp.32-43
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• 2000

In this paper, we investigate the existing medium access control (MAC) protocols to integrate the voice and data services in packet-based CDMA networks and furthermore, propose a new approach to circumvent the operational limits inherent in them. We propose the $P^2R$-CDMA (Prioritized Packet Reservation Code Division Multiple Access) protocol for the uplink in the synchronous multi-code CDMA system, which employs the centralized frame-based slot reservation along with the dynamic slot assignment in the base station using the QoS-oriented dynamic priority of individual terminal. The simulation results show that, as compared with the existing scheme based on the adaptive permission probability control (APC), the proposed approach can significantly improve the system capacity while guaranteeing the real-time requirement of voice service.

• Journal of Industrial Technology
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• v.21 no.A
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• pp.189-198
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• 2001

In recent years, the predominant part in telecommunications is mobile communications. The next generation network is extending today's voice-only mobile networks to multi-service networks. ATM Network is possible to carry such multi-media traffic and it will be expect to use wireless ATM for the future mobile access network. One of manly important aspects for the performance of wireless ATM is the Medium Access Control (MAC) protocol. The MAC protocol must be able to satisfyingly handle the different ATM services (CBR, VBR, ABR and UBR) with their radically different performance requirements. Additionally, the MAC protocol must be able to cope with the complex radio environment where fading, multi-path propagation interference and burst-errors further complicate the situation. In this paper, a dynamic slot allocation algorithm in wireless ATM is proposed for an efficient channel sharing/media access at the MAC layer. We use equivalent capacity in the allocation of slots for VBR traffic which is variable along the time. It is simple and effective slot allocation method for VBR service. In particular, we consider the slot allocation of a session consisted of several connections for requirement of multimedia traffic. Simulation shows that the cell loss ratio is reduced by re-allocation of extra slots in Mobile Terminal (MT).

• The Journal of Asian Finance, Economics and Business
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• v.7 no.9
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• pp.341-350
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• 2020

This study aims to verify the relationship between overseas activities and performance of Korea's SMEs during the last financial crisis. Whether overseas activity performance of enterprises differed was determined based on characteristic variables, including the degree of concentration on R&D and marketing. This study also examined how SMEs' international transactions and their performance differed based on internal variable such as the level of stock holding and firm size. This study developed a model for analyzing the relationship between the level of internationalization and performance of Koran SMEs listed in the KOSDAQ. We used firm-level data, including annual reports and various data sources such as the KISVALUE program. To smooth annual fluctuations in accounting data, we used a three-year average from 2006 to 2009 for each variable in the study. The results showed that proactive overseas activities ultimately had a positive effect on an enterprise's performance, even though it initially had a negative effect. Therefore, enterprises should focus their capacity on R&D and marketing environment. Although numerous studies have focused on the relationship between overseas activities and performance of enterprises, the present study analyzed whether enterprises should continuously engage in overseas activities and what capacities they should strengthen during a global economic recession.

• BMB Reports
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• v.37 no.6
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• pp.735-740
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• 2004

Hepatitis C virus (HCV) is a causal agent of the chronic liver infection. To understand HCV morphogenesis, we studied the assembly of HCV structural proteins in insect cells. We constructed recombinant baculovirus expression vectors consisting of either HCV core alone, core-E1, or core-E1-E2. These structural proteins were expressed in insect cells and were examined to assemble into particles. Neither core-E1 nor core-E1-E2 was capable of assembling into virus-like particles (VLPs). It was surprising that the core protein alone was assembled into core-like particles. These particles were released into the culture medium as early as 2 days after infection. In our system, HCV structural proteins including envelope proteins did not assemble into VLPs. Instead, the core protein itself has the intrinsic capacity to assemble into amorphous core-like particles. Furthermore, released core particles were associated with HCV RNA, indicating that core proteins were assembled into nucleocapsids. These results suggest that HCV may utilize a unique core release mechanism to evade the hosts defense mechanism, thus contributing to the persistence of HCV infection.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.594-601
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• 2010

Azotobacter chroococcum H23 (CECT 4435), Azotobacter vinelandii UWD, and Azotobacter vinelandii (ATCC 12837), members of the family Pseudomonadaceae, were used to evaluate their capacity to grow and accumulate polyhydroxyalkanoates (PHAs) using two-phase olive mill wastewater (TPOMW, alpeorujo) diluted at different concentrations as the sole carbon source. The PHAs amounts (g/l) increased clearly when the TPOMW samples were previously digested under anaerobic conditions. The MNR analysis demonstrated that the bacterial strains formed only homopolymers containing $\beta$-hydroxybutyrate, either when grown in diluted TPOMW medium or diluted anaerobically digested TPOMW medium. COD values of the diluted anaerobically digested waste were measured before and after the aerobic PHA-storing phase, and a clear reduction (72%) was recorded after 72 h of incubation. The results obtained in this study suggest the perspectives for using these bacterial strains to produce PHAs from TPOMW, and in parallel, contribute efficiently to the bioremediation of this waste. This fact seems essential if bioplastics are to become competitive products.

• Journal of Embryo Transfer
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• v.23 no.2
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• pp.77-80
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• 2008

In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrified-thawed porcine oocytes were examined. Also, the developmental capacity of vitrified-thawed immature porcine oocytes following ICSI was investigated. Oocytes were cultured in NCSU-23 medium supplemented with 5% FBS at $38^{\circ}C$ in 5% $CO_2$ and air. The in vitro maturation rate of vitrified-thawed oocytes ($24.1{\pm}2.5%$) was lower than that of the control ($46.0{\pm}3.2%$, p<0.05). The in vitro maturation rate of vitrified-thawed oocytes treated with $1.0{\sim}5.0\;ug$ CB + NCSU- 23 medium were $22.2{\pm}3.0%$, $30.7{\pm}3.2$, $46.3{\pm}3.1%$, $38.5{\pm}3.2%$, respectively. The in vitro maturation rate ($46.3{\pm}3.4%$) of the vitrified-thawed oocytes treated with $3.0\;{\mu}g$ CB for 30 min was the highest of all vitrification groups. When the in vitro developmental rates of the vitrified-thawed (with EDS and EDT) oocytes following ICSI were $18.5{\pm}2.5%$, $16.4{\pm}2.1%$, respectively. This results were lower than the control group ($24.0{\pm}2.5%$).

• Journal of Korean Society on Water Environment
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• v.23 no.1
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• pp.155-160
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• 2007

Constructed wetland has been widely used for the treatment of sewage, stormwater runoff, industrial wastewater, agricultural runoff, acid mine drainage and landfill leachate. For the removal of nitrogen and phosphorus, uptake by plants and adsorption to media material are the major processes, and, therefore, the selection of media with specific adsorption capacity is the critical factor for the optimal design of wetland along with the selection of appropriate plant species. In this study, two media materials (loess bead and mixed media) possessing different adsorption characteristics for ammonium and phosphate were selected, and their adsorption characteristics were evaluated. In addition, the performance of subsurface-flow wetland systems employing these media was evaluated in both batch and continuous flow systems. With LB medium, beter phosphorus removal was observed, while better ammonia removal was obtained with MM medium. In addition, enhanced removal efficiencies were observed in the wetland systems employing both media and aquatic plants, mainly due to the better environment for microbial growth. As a result, appropriate selection or combination of media with respect to the inflow water quality maybe important factors for the successful design and operation of wetland systems.

• Journal of The Korean Society of Agricultural Engineers
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• v.50 no.6
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• pp.3-12
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• 2008

It is important to estimate accurate effective rainfall to analyse flood flow and long-term runoff for the rational planning, design, and management of water resource. The initial abstraction is also important to estimate effective rainfall. The Soil Conservation Service (SCS) has developed a procedure and it has been most commonly applied to estimate effective rainfall. But the SCS method still has weak points, because of unnatural assumptions such as antecedent moisture conditions and initial abstraction. The coefficient of initial abstraction(K) is depending on the soil moisture condition and antecedent rainfall. The maximum storage capacity of Umax which is calibrated by stream flow data in the proposed watershed was derived from the DAWAST(DAily WAtershed STreamflow) model. The values of K obtained from 69 storm events at the five watersheds are ranging from 0.133 to 0.365 and its mean value is 0.207. Effective rainfall could be estimated more reasonably by introducing new concept of initial abstraction. The equation of $K=0.076Sa^{0.255}$ was recommended instead of 0.2 and it could be applicable to the small-medium rural watersheds.