• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1101-1107
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• 2015

The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.419-427
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• 1991

Ethanol fermentation of glucose by a strain of Saccharomyces cereuisiae was studied in membrane recycle bioreactor, where the fermentation vessel was coupled with cross flow hollow fiber membrane. The cell recycle system controlled backflushing with fresh medium was proven to be effective in alleviating membrane fouling and allowing long term operation of high-cell continuous fermentation. Using 100 g/l initial glucose concentration, the maximum productivity of about 9 5 g/$l \cdot h$ has been achieved at dilution rate 2.5 $h^{-1}$ and bleed stream ratio 0.05 with the corresponding ethanol concentration of 35g/l and glucose conversion of 100%. Increasing the glucose concentration to 200 g/$l \cdot h$ resulted in an increase in ethanol concentration to 48 g/l and productivity to 120 g1l.h. Substrate conversion, however, was only 69%. This productivity was the highest value in the study, and about 38 fold more than that of batch culture and 17 fold more that of single stage continuous culture without cell recycling. No further increase in the productivity was obtained when the glucose concentration was increased reased to 300g/l.

• Korean Journal of Optics and Photonics
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• v.20 no.3
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• pp.189-194
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• 2009

We demonstrate a ring type wavelength swept laser incorporating a fiber Fabry-Perot tunable filter in a laser cavity using 1300 nm semiconductor optical amplifier as a gain medium. The output characteristics of the wavelength swept laser according to the applied scanning frequencies are analyzed in the temporal and spectral domain. The output of the wavelength swept laser decreases dramatically as the scanning frequency increases. And there is a significant peak power imbalance between the forward scan and the backward scan as the scanning frequency increases. Its use in practical applications might be limited.

• Korean Chemical Engineering Research
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• v.44 no.5
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• pp.468-475
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• 2006

For the filtration of super compactible cake, the high filtration pressure can not improve filtration rate. As the high pressure, in this case, decreases the cake porosity adjacent to filter medium and thus forms 'dense skin' which decreases the rate of liquid flow in a great extent. Actually, there was no method to improve filtration rate for the filtration with super compactible cake. We propose the saturation of $CO_2$ gas into the suspension before the filtration operation for improving the filtration rate. The dissolved $CO_2$ gas transforms itself into gas phase in the dense skin through which the pressure changes dramatically. The gas secures its space inside the dense skin, and finally forms the flow passages which improve the filtration rate.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.183-186
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• 1994

Somatic embryos derived from cell suspension cultures of rice were singly alginate-encapsulated to be used as artificial seeds. When placed on half strength MS solid medium,73% of the encapsulated somatic embryos were capable of germination Encapsulation per se did not affect the germination frequency of embryos. When incubated by wrapping with moistured non-sterile filter paper, 60% of the encapsulated somatic embryos germinated. However encapsulated zygotic embryos without endosperm showed a high germination frequency regardless of the sterility of the incubation conditions. The results suggest that a greater susceptibility of somatic embryos to contaminants is attributed to lower germination frequency of encapsulated somatic embryos in non-sterile conditions.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.9 no.2
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• pp.79-89
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• 1999

A quantitative fit test, condensation nuclei counting (Portacount 8025, TSI), was performed concurrently with a banana oil (isoamyl acetate: IAA) qualitative fit test (MSA) to evaluate reliability on IAA QLFT and correlation between two methods. One brands of quarter mask (3M model 7500 medium) was prepared for QLFT with HEPA filter and gas & vapor removing media, i.e., combination cartridge. 110 subjects (65 male, 45 female) were fit tested QNFT and QLFT each three times. For a wearer combination having a FF<10, as determined by CNC QNFT, the point es timate (${\beta}$-error) of the probability of that combination not being rejected by the banana oil QLFT was found to be 0.0 with 95% confidence that this statistic is not expected to exceed 0.15. For a wearer combination having a FF<100, as determined by CNC QNFT, the point estimate of the probability of that combination not being rejected by the banana oil QLFT was found to be 0.07 with 95% confidence that this statistic is not expected to exceed 0.13. The uncertainty associated with each estimate, however, is large due to the small number of study subjects with inadequately fitting respirators.

• The Plant Pathology Journal
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• v.19 no.6
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• pp.280-283
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• 2003

A soybean-sprout rot epidemic occurred in a mass production soybean sprout factory in 2000 and 2001 in Korea, which caused up to 20% production loss. Among the causal pathogenic bacteria and fungi, Pythium deliense was found to be the dominant pathogen of severe root and hypocotyls rot, particularly in recirculating water system. An average of 90% of the isolated fungi from the rotted sprout on potato dextrose agar were Pythium sp. The fungal density of Pythium in the sampled water was monitored in the recycled water system for 1 year using a selective medium (com meal agar with Pimaricin, 10 mg； Rifampicin, 10 mg； and Ampicillin, 100 mg per 1 liter). The drained water from the soybean-sprout cultivation always had a certain amount of fungus in it. The removal of Pythium from the recycling water system must be thorough, safe, and environment friendly. However, the pathogen in the water was easily found even after ozone and chlorine treatments, which were devised on the recycling system for the removal of microorganisms. 5-$\mu\textrm{m}$ pore size filter was applied and was able to successfully control the disease. As the sprout industry increasingly shifts into mass production, the demand for water will increase continuously. Recycling water for sprout production is eco-friendly. However, a process must be devised to be able to first decompose organic matters before Pythium zoospores are filtered.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.11
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• pp.4103-4117
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• 2014

Image processing and computer vision algorithms are gaining larger concern in a variety of application areas such as robotics and man-machine interaction. Vision allows the development of flexible, intelligent, and less intrusive approaches than most of the other sensor systems. In this work, we determine the location and orientation of a mobile robot which is crucial for performing its tasks. In order to be able to operate in real time there is a need to speed up different vision routines. Therefore, we present and evaluate a method for introducing parallelism into the multiple non-overlapping camera pose estimation algorithm proposed in [1]. In this algorithm the problem has been solved in real time using multiple non-overlapping cameras and the Extended Kalman Filter (EKF). Four cameras arranged in two back-to-back pairs are put on the platform of a moving robot. An important benefit of using multiple cameras for robot pose estimation is the capability of resolving vision uncertainties such as the bas-relief ambiguity. The proposed method is based on algorithmic skeletons for low, medium and high levels of parallelization. The analysis shows that the use of a multiprocessor system enhances the system performance by about 87%. In addition, the proposed design is scalable, which is necaccery in this application where the number of features changes repeatedly.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.2
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• pp.76-84
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• 2002

On-line conversion estimation of enzymatic esterification reactions in solvent-free media was investigated. In principle, conversion to ester can be determined from the amount of water produced by the reaction, because water is formed as a by-product in a stoichiometric manner. In this study, we estimated the water production rate only from some measurements of relative humidity and water balances without using any analytical methods. In order to test the performance of the on-line conversion estimation, the lipase-catalyzed esterification of n-capric acid and n-decal alcohol in solvent-free media was performed whilst controlling water activity at various values. The reaction conversions estimated on-line were similar to those determined by offline gas chromatographic analysis. However, when the water activity was controlled at higher values, discrepancies between the estimated conversion values and the measured values became significant. The deviation was found to be due to the inaccurate measurement of the water content in the reaction medium during the initial stages of the reaction. Using a digital filter, we were able to improve the accuracy of the on-line conversion estimation method considerably. Despite the simplicity of this method, the on-line estimated conversions were in good agreement with the off-line measured values.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.2
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• pp.24-29
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• 2009

Sound source localization system is used in a robot, a video conference and CCTV(Closed-circuit television) systems. In this Sound source localization systems are applied to human and they can receive a number of sound data frames during speaking. In this paper, we propose methods which is reducing angle estimation error by selecting sound data frame which can more precisely compute the angles from inputted sound data frame. After selected data converted to angle, the error of sound source localization recognition system can be reduced by applying to medium filter. By the experiment using proposed system it is shown that the average error of angle estimation in sound source recognition system can be reduced up to 31 %.