• 한국식품영양학회지
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• 제25권2호
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• pp.308-316
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• 2012

Proper skin care promotes good metabolism and the biological activity of skin, helps maintain the skin in a healthy and beautiful state when combined with nutritional nourishment. Health functional foods are being used for the purpose of enhancing efficient skin care. Health functional foods related to skin care maintain the epidermis, dermal fibroblast layer and subcutaneous tissue that form the skin. Efficient functional foods alleviate the signs of endogenous aging that come with getting older and exogenous aging caused by sunlight. Even though the field of skin care related to health functional foods has received less attention and been the subject of less research compared to functional cosmetics which are developing widely, this area of skin care that maintains and improves the layer of dermal fibroblast through the intake of food, is expected to progress with the commercialization of products in many fields when the related technical research development is galvanized and the related patents are applied for. Research into health functional foods related to skin care in Korea started rather late in comparison to other advanced countries where patents for relevant techniques have been applied for since 1990's together with research conducted on how they might be used for practical purposes. This study used the key words 'skin care, health functional food' to search for Korean patents that have been applied for at the Korean Intellectual Property Office from 1983 to 2011 using the KIPRIS database, in order to help researchers in the related fields by organizing the patented formulas for health functional foods for skin care which have gained attention recently. According to the number of registrations in each industry field, the total number of patents was 1,120 we screened the major patents among them, the field with the largest number was food foodstuff non-alcoholic beverage and preparation or treatment(A23L) with 135, then natural plant medicine & medical supplies and cosmetics(A61K) with 112, microorganisms or enzymes(C12N) with 63, heterocyclic compounds(C07D) with 44, horticulture or cultivation of sea weeds, forestry(A01G) 16 times, listed from the highest to the lowest number of applications. It has been revealed that food, foodstuff, non-alcoholic beverage and preparation or treatment(A23L) was the industry field where the most patents for health functional foods related to skin care were registered.

• Genomics & Informatics
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• 제16권3호
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• pp.52-58
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• 2018

In this report, we present a case study of how pharmacogenomics and pharmacometabolomics can be useful to characterize safety and pharmacokinetic profiles in early phase new drug development clinical trials. During conducting a first-in-human trial for a new molecular entity, we were able to determine the mechanism of dichotomized variability in plasma drug concentrations, which appeared closely related to adverse drug reactions (ADRs) through integrated omics analysis. The pharmacogenomics screening was performed from whole blood samples using the Affymetrix DMET (Drug-Metabolizing Enzymes and Transporters) Plus microarray, and confirmation of genetic variants was performed using real-time polymerase chain reaction. Metabolomics profiling was performed from plasma samples using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A GSTM1 null polymorphism was identified in pharmacogenomics test and the drug concentrations was higher in GSTM1 null subjects than GSTM1 functional subjects. The apparent drug clearance was 13-fold lower in GSTM1 null subjects than GSTM1 functional subjects (p < 0.001). By metabolomics analysis, we identified that the study drug was metabolized by cysteinylglycine conjugation in GSTM functional subjects but those not in GSTM1 null subjects. The incidence rate and the severity of ADRs were higher in the GSTM1 null subjects than the GSTM1 functional subjects. Through the integrated omics analysis, we could understand the mechanism of inter-individual variability in drug exposure and in adverse response. In conclusion, integrated multi-omics analysis can be useful for elucidating the various characteristics of new drug candidates in early phase clinical trials.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.378-384
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• 2012

In aquatic invertebrates, particularly marine gastropods, organotin compounds induce irreversible sexual abnormality in females, which is termed imposex, at very low concentrations. Organotin compounds are agonists for nuclear receptors such as RXRs and $PPAR{\gamma}$. However, the imposex phenomenon has not been reported to act as an antagonist on estrogen receptors in other species, including vertebrates and invertebrates. In order to gain insights into the antagonistic activity of organotin compounds on estrogen receptors (ERs), we examined the inhibitive effect of these compounds on estradiol-dependent ${\beta}$-galactosidase activity using the yeast two-hybrid detection system consisting of a combination of the human estrogen receptor ($hER{\beta}$) ligand-binding domain and the co-activator steroid receptor co-activator-1 (SRC1). Tributyltin-hydroxide (TBT-OH) and triphenyltin-chlorine (TPT-Cl) exhibited an inhibitive effect on $E_2$-dependent transcriptional activity, similar to antagonistic chemicals such as 4-hydroxytamoxifen (OHT) or ICI 182,780, at a very low concentration of $10^{-14}$ M TBT or $10^{-10}$ M TPT, respectively. The yeast growth and transcriptional activity with transcriptional factor GAL4 did not exhibit any effect at the tested concentration of TBT or TPT. Moreover, the yeast two-hybrid system using the interaction between p53 and the T antigen of SV40 large did not describe any effect at the tested concentration of OHT or ICI 182,780. However, the interaction between p53 and T antigen was inhibited at a TBT or TPT concentration of $10^{-9}$ M, respectively. These results indicate that TBT and TPT act as inhibitors of ER-dependent reporter gene transcriptional activation and of the interaction between $hER{\beta}$ LBD and the co-activator SRC1 in the yeast two-hybrid system. Consequently, our data could partly explain the occurrence of organotin compound-induced imposex on the endocrine system of mammals, including humans.

• 지역사회간호학회지
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• 제15권4호
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• pp.551-565
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• 2004

Purpose: The purpose of this study is to develop a day care center model focused on public health institutions for the elderly residing in their homes. Method: Research design for this study was a mult-level research, which consisted of a related literature review, an Internet search for knowledge of the current situation at home and abroad, on-site interviews, questionnaires collected from a sample of residents in a rural area, and a key-informants approach. Results: 1) The subjects of service - Generalized service should be provided to the elderly, 65 years and older, regardless of their assets. 2) The contents of service - Providing pre-health oriented and post-social welfare service that can integrate and satisfy a wide variety of public health and welfare needs of the elderly would strengthen the health care service of a day care center for the elderly. 3) Delivery system - Basic-level local self-governments should become a central operating body, and establishing a properly adjusted delivery system to a rural area after considering the efficiency and the access of vulnerable rural areas is needed based on modification of 'a Special Law for Agricultural and Fishery Areas' (rural public health center>rural health sub-center ${\rightarrow}$ unified health sub-center ${\rightarrow}$ public health hospital (public health center) ${\rightarrow}$ public welfare office). 4) Facility - Public health facilities such as public health centers and sub-centers should be located in areas that can easily access the facilities. 5) Funding - For day care center for the elderly in local self-government, the central government should modify a relevant implementation of subsidy in and provide some facilities and service regardless of the degree of self reliance of local self-government. 6) Human resources - It is needed to guarantee the period of workers of a day care center for the elderly, at least 3 to 5 years, with considering their specialty on aged care and avoiding circulation based positions. Furthermore, appropriate specially trained personnel such as medical workers and social workers should be placed to take care of both health service and welfare through strengthening of 'rules of law of elderly welfare,' Conclusion: future research is needed to test the model through a demonstration study using a model which may be developed in the future and to standardize the appraisal criteria of people hoping to enter a day care center for the elderly.

• Journal of Oral Medicine and Pain
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• 제30권1호
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• pp.35-44
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• 2005

본 예비연구는 강의중심학습 (LBL) 방식 과 문제중심학습 (PBL) 방식이 기초의학 지식의 이해력의 향상에 미치는 영향을 사후-사전 검사의 변화량의 차이의 비교를 통해서 그리고 과학적 추론을 동반한 반 구조화된 문제해결의 질에 미치는 영향을 사후 검사의 차이의 비교를 통해서 평가하는 것이 목적이다. 이 반 구조화된 문제는 다섯 개- 샘플 크기, 원인과 결과의 연구설계, 위험요인 구성, 데이터의 통계 분석, 결과 해석- 의 요소를 포함하고 있다. 본 실험은 2004년 이 주에 걸쳐 서울대학교 치과대학 100명의 학부 학생들을 대상으로 실시되었다. 상이한 두 학습들이 실시되기 전 과 후에 감염방지와 예방에 관한 48개의 사지 혹은 오지 선다형의 문제가 학생들에게 주어졌다. 또 두 학습이 종결된 후에 역학관련 반 구조화된 문제를 해결하도록 하는 과제를 통하여 문제해결의 능력에 미치는 두 학습 방식의 영향을 분석하였다. 사전-사후 테스트의 결과 기초의학 지식의 이해 향상 정도는 두 그룹 간의 유 의미한 차이를 보이지 않았다. 반면, 반 구조화된 문제해결의 질은 문제중심학습 방식이 유 의미하게 높았으며 (p=.029), 특히 문제 해결 과정에서 나타난 인과성 연구설계 (p=.000)와 결과 해석(p=.001)에 관한 과학적 추론의 점수가 유 의미하게 우수하였다. 결론적으로 동일한 시간 동안 문제중심학습 방식은 강의중심학습 방식에 비해 하위 기초지식의 이해를 위한 학습 환경에서 불리하지 않았을 뿐만 아니라 상위 인지 활동인 문제해결 과정에서 과학적 추론을 개발하는데 공헌 하였다.

• 대한장애인치과학회지
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• 제12권2호
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• pp.96-100
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• 2016

저자는 후기 영아형 이염성 백질 이영양증으로 인해 신경학적인 퇴행이 관찰되고 섭식 장애로 인한 다발성 우식을 보이는 환아를 전신마취 하 치료하였기에 문헌 고찰과 함께 보고하는 바이다. 저작기능 장애로 인한 영양 결핍으로 환아는 신체 발육이 매우 저하되었으며 긴 식사 시간으로 다발성 치아 우식에 이환된 상태였다. 협조도 부족 및 치료 범위 등을 고려하여 전신마취 하에 치과 치료를 시행하였고 정기적인 검진을 시행 중이다. 진행성, 신경성 퇴행질환의 특징을 이해하고 환아의 상태를 고려한 구강 건강 관리 및 보호자 교육을 제공하는 것이 필요할 것으로 사료된다.

• 과학기술학연구
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• 제12권1호
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• pp.1-43
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• 2012

이 글은 BRCA1과 BRCA2 두 인간유전자에 관련된 특허들에 대한 최근의 소송에서 제기된 인간유전자 특허의 여러 경제적, 법률적, 그리고 윤리적 쟁점들을 분석한다. 기존의 인간유전자 특허관련 연구들이 이의 법률적 논리와 윤리적 정당성에 대한 규범적인(normative) 분석에 초점을 두었다면, 이 글은 BRCA 관련 특허소송에의 기저에는 반공유재의 비극(the tragedy of the anticommons)이라 불리는 지적재산권과 경제혁신, 공공의 이익의 관계에 대한 새로운 이해가 존재하고 있음을 보일 것이다. 첫 부분에서는 2001년 미 특허청의 인간유전자 특허에 대한 정책의 형성과정에 대한 역사적인 분석을, 다음으로는 생명과학에서 지적재산권의 확대를 가져온 여러 경제적 가정들과 제도적 변화, 그리고 법적 판결들에 대한 비판적 논의를 소개할 것이다. 지적재산권의 한계와 생명의 사유화에 대한 비판을 통해서 BRCA 소송은 지적재산권의 정의와 그 범주, 그리고 이의 소유권을 둘러싼 논쟁이 공공의 이익, 과학과 의학 공동체의 창조적 지적활동과 환자들의 인권과 윤리의 문제가 복잡다단하게 얽혀있음을 보여주고 있다.

• 농촌계획
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• 제22권2호
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• pp.121-129
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• 2016

A disaster can be defined in many ways based on perspectives, in addition, its types are able to classify differently by various standards. Considering the different perspectives, the disaster can be occurred by natural phenomenon that is like typhoon, earthquake, flood, and drought, and by the accident that is like collapse of facilities, traffic accidents, and environmental pollution, etc. Into the modern society, moreover, the disaster includes the damages by diffusion of epidemic and infectious disease in domestic animals. The disaster was defined by natural and man-made hazards in the past. As societies grew with changes of paradigm, social factors have been included in the concept of the disaster according to new types unexpected by new disease and scientific technology. Change the concept of social disasters, Ministry of Public Safety and Security (MPSS) has provided the regional safety index, which measures the safety level of a local government. However, this regional safety index has some limitation to use because this index provides the information for city unit which is a unit of administrative districts of urban. Since these administrative districts units are on a different level with urban and rural areas, the regional safety index provided by MPSS is not be able to direct apply to the rural areas. The purpose of this study is to determine the regional safety index targeting rural areas. To estimate the safety index, we was used for 3 indicators of the MPSS, a fire, a crime, and an infectious disease which are evaluable the regional safety index using an accessibility analysis. For determining the regional safety index using accessibility from community centers to public facilities, the safety index of fire, crime, and infectious disease used access time to fire station, police office, and medical facility, respectively. An integrated Cheongju, targeting areas in this study, is mixed region with urban and rural areas. The results of regional safety index about urban and rural areas, the safety index in rural area is relatively higher than in the urban. Neverthless the investment would be needed to improve the safety in the rural areas.

• 한국균학회지
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• 제38권2호
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• pp.146-151
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• 2010

검체는 2005년 1월1일부터 2009년 12월31일까지 C 병원 진단 검사의학과에 의뢰된 환자의 임상검체 총 366,661예로부터 총 167,955 미생물이 분리되었고 이 중 6,517주의 Candida spp. 분리균을 자동화기기로 동정한 것을 후향적으로 처리 하였다. 배양균주는 smooth하고, 크림색이며, 약간 mucoid하고, 혼탁한 집락을 0.45% salin에 McFarland No 1.6-2.0되게 탁도를 맞춘 후 반응시약이 들어 있는 ID-YST card에 접종하여, VITEK II(bioMerieux Duham NC, USA)의 자동화기기에 넣어 동정하였고, 그 결과는 다음과 같다. Candida spp.의 분리율은 2005년도에 비하여 2009년이 1.9배 증가하였다. 분리빈도가 높은 Candida spp.는 C. albicans 56.4%, C. tropicalis 17.7%, C. glabrata 10.7%, C. parapsilosis 9.5%였고, Candida spp가 10%이상 분리된 검체는 객담 30.1%, 무작위소변 25.0%, 혈액 15.8%, 카테타소변 13.5%였으며, 7%이상 분리된 부서는 호흡기내과 20.5%, 신장내과 11.0%, 감염내과 10.4%, 응급의학과 10.0%, 혈액종양내과 9.6%, 외과 7.5%, 소화기내과 7.4%였다. Candida spp가 증가된 달은 7월이 10.6%였고, 가장 낮게 분리된 달은 2월이 6.1%였다. Candida spp.가 증가된 연령군은 70세 이상 군이 40.1%였고, 가장 낮게 분리된 연령군은 10대가 1.3%였고, 0-49세군(1.3-7.5%)대 50세 이상 군(16.7-40.1%)의 비교에서는 1 : 4.7로 50세 이상 군이 월등히 높았다.

• Journal of Preventive Medicine and Public Health
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• 제39권4호
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• pp.353-358
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• 2006

Objectives: An increase in the serum gamma-glutamyltransferase (GGT) concentration has been regarded as a marker of alcohol drinking or liver disease. Some reports, however, have suggested that the serum GGT may be a sensitive and early biomarker for the development of prediabetes and diabetes. In this study we investigated whether serum GGT is a reliable predictor of the incident impaired fasting glucose (IFG), including diabetes. Methods: We performed a prospective study for two years (2002-2004). We analyzed the periodic health examination data from a total of 4,711 men. The examinations were done in the years 2002 and 2004. The analyzed data included a self-questionnaire, a physical examination and the laboratory results. Both IFG and diabetes were defined as a serum fasting glucose concentration of more than 100 mg/dL and 126 mg/dL, respectively. Results: A total of 738 cases (15.7%) of incident IFG and 13 cases (0.3%) of diabetes occurred. The mean serum GGT concentrations were quite different between the normal (38.0 IU) and incident IFG groups (50.3 IU), and the incident diabetes group (66.0 IU) (p<0.001). After multivariable adjustment, the relative risks for incident IFG or diabetes across the baseline GGT categories (<10th, 10th-20th, 30th-40th, 50th-60th, 70th-80th and >90th percentile) were 1.0, 1.172 (0.769-1.785), 1.107 (0.725-1.689), 1.444 (0.934-2.232), 2.061 (1.401-3.031) and 2.545 (1.784-3.631) (p-value for trend: <0.001). The risks significantly increased with increasing levels of GGT for 2 years; when comparing the increased groups (<10%, 10-20%, >20%) versus the decreased over 20% group of GGT, the risks for IFG or diabetes were 1.334 (1.002-1.776), 1.613 (1.183-2.199) and 1.399 (1.092-1.794). Conclusions: Our findings suggest that serum GGT concentrations within its normal range may be an early predictor of the development of IFG and diabetes. As serum GGT is a relatively inexpensive test and a reliable marker, it might have important implications in public health promotion.