• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.2
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• pp.156-163
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• 2008

Luminescent bacterial toxicity test was first introduced in the early 1980s, registered as international standard method in 1998 and now widely used as a common toxicity test method. This toxicity test uses luminescent bacterium, Vibrio fischeri, originated from marine environment as a test organism. The degree of toxicity can be evaluated from the comparison of luminescent emission intensity between control and treatment groups to toxicants and materials from various environmental matrix for 30 min. This test can be carried out by using commercial products and its results are sensitive and precise. This research is on the feasibility of adopting luminescent bacterial test as a domestic standard test protocol. Using commercial products, a series of experiments were conducted to identify the precision and accuracy of injection volume and light emission, and to evaluate concentration-response relationship between chemical concentrations and light emissions. Also, the feasibility of the application to environmental media and quality assurance/quality control were checked. The results of serial toxicity tests revealed that the preliminary luminescent bacterial toxicity test was robust and suitable as a standard method.

• Korean Journal of Radiology
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• v.20 no.4
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• pp.621-630
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• 2019

Objective: To study the prevalence and clinical characteristics of decreased myocardial blood flow (MBF) quantified by dynamic computed tomography (CT) myocardial perfusion imaging (MPI) in symptomatic patients without in-stent restenosis. Materials and Methods: Thirty-seven (mean age, 71.3 ± 10 years; age range, 48-88 years; 31 males, 6 females) consecutive symptomatic patients with patent coronary stents and without obstructive de novo lesions were prospectively enrolled to undergo dynamic CT-MPI using a third-generation dual-source CT scanner. The shuttle-mode acquisition technique was used to image the complete left ventricle. A bolus of contrast media (50 mL; iopromide, 370 mg iodine/mL) was injected into the antecubital vein at a rate of 6 mL/s, followed by a 40-mL saline flush. The mean MBF value and other quantitative parameters were measured for each segment of both stented-vessel territories and reference territories. The MBF_ratio was defined as the ratio of the mean MBF value of the whole stent-vessel territory to that of the whole reference territory. An MBF_ratio of 0.85 was used as the cut-off value to distinguish hypoperfused from non-hypoperfused segments. Results: A total of 629 segments of 37 patients were ultimately included for analysis. The mean effective dose of dynamic CT-MPI was 3.1 ± 1.2 mSv (range, 1.7-6.3 mSv). The mean MBF of stent-vessel territories was decreased in 19 lesions and 81 segments. Compared to stent-vessel territories without hypoperfusion, the mean MBF and myocardial blood volume were markedly lower in hypoperfused stent-vessel territories (77.5 ± 16.6 mL/100 mL/min vs. 140.4 ± 24.1 mL/100 mL/min [p < 0.001] and 6.4 ± 3.7 mL/100 mL vs. 11.5 ± 4 mL/100 mL [p < 0.001, respectively]). Myocardial hypoperfusion in stentvessel territories was present in 48.6% (18/37) of patients. None of clinical parameters differed statistically significantly between hypoperfusion and non-hypoperfusion subgroups. Conclusion: Decreased MBF is commonly present in patients who are symptomatic after percutaneous coronary intervention, despite patent stents and can be detected by dynamic CT-MPI using a low radiation dose.

• Journal of Animal Environmental Science
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• v.9 no.2
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• pp.93-102
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• 2003

This study designed and constructed an experimental column far adhesion efficiency test and conducted experiment to investigate the offensive odor adhesion efficiency of filter bed materials. The offensive odor adhesion experiment was conducted using mixture of high physical adhesion efficiency material, and the fixity of deodorization microorganism of selected filter bed material was tested using ammonia exclude microorganism A4-­2 and sulfur oxidation microorganism S5­-5.2 those were cultured at the Agricultural Chemical Department of Chungnam National University, and deodorization efficiency of selected filter bed material mixture was tested. Following are summary of these tests results. 1. Amount of elimination of the offensive odor gas of ammonia and hydrogen sulfide per unit volume was 0.054 and 0.016$\ell/\textrm{cm}^3$ in rice hull, 0.01 and 0.004 $\ell/\textrm{cm}^3$ in rice straw 0.158 and 0.01 $\ell/\textrm{cm}^3$ in coconut, 0.014 and 0.02$\ell/\textrm{cm}^3$ perlite, 0.004 and 0.003$\ell/\textrm{cm}^3$ in high road ball, and 0.112 and 0.015 $\ell/\textrm{cm}^3$ in chaff of pine, respectively. 2. Amount of elimination of offensive odor gas of ammonia and hydrogen sulfide per unit volume was 0.045 and 0.014$\ell/\textrm{cm}^3$ in mixture 1, 0.079 and 0.016$\ell/\textrm{cm}^3$ in mixture 2, 0.123 and 0.017 $\ell/\textrm{cm}^3$ in mixture 3, 0.031 and 0.015$\ell/\textrm{cm}^3$ in mixture 4, 0.055 and 0.016$\ell/\textrm{cm}^3$ in mixture 5, and 0.111 and 0.020$\ell/\textrm{cm}^3$ in mixture 6, respectively. 3. The offensive odor elimination microorganism inoculated to mixture of chaff of pine(70%) and perlite(30%) showed the elimination efficiency of 99.06% and 96.61% against the ammonia and hydrogen sulfide, respectively, during 24 hours period.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.424-431
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• 2018

Stormwater runoff containing considerable amounts of pollutants such as particulates, organics, nutrients, and heavy metals contaminate natural bodies of water. At present, best management practices (BMP) intended to reduce the volume and treat pollutants from stormwater runoff were devised to serve as cost-effective measures of stormwater management. However, improper design and lack of proper maintenance can lead to degradation of the facility, making it unable to perform its intended function. This study evaluated an infiltration trench (IT) that went through a series of maintenance operations. 41 monitored rainfall events from 2009 to 2016 were used to evaluate the pollutant removal capabilities of the IT. Assessment of the water quality and hydrological data revealed that the inflow volume was the most relative factor affecting the unit pollutant loads (UPL) entering the facility. Seasonal variations also affected the pollutant removal capabilities of the IT. During the summer season, the increased rainfall depths and runoff volumes diminished the pollutant removal efficiency (RE) of the facility due to increased volumes that washed off larger pollutant loads and caused the IT to overflow. Moreover, the system also exhibited reduced pollutant RE for the winter season due to frozen media layers and chemical-related mechanisms impacted by the low winter temperature. Maintenance operations also posed considerable effects of the performance of the IT. During the first two years of operation, the IT exhibited a decrease in pollutant RE due to aging and lack of proper maintenance. However, some events also showed reduced pollutant RE succeeding the maintenance as a result of disturbed sediments that were not removed from the geotextile. Ultimately, the presented effects of maintenance operations in relation to the pollutant RE of the system may lead to the optimization of maintenance schedules and procedures for BMP of same structure.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.11
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• pp.1935-1944
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• 2000

This research was performed to investigate the effects of the location of recirculation port on the wastewater treatment efficiency of an anaerobic hybrid reactor consisted of a fluidized bed and a packed bed. The recirculation port was located either at the top of the packed bed (Reactor 2) or above the fluidized bed (Reactor 1). Media for the fluidized bed and the packed bed were granular activated carbon and Pall ring-type plastic media. respectively. At organic loading rates(OLR) up to $6.2kg\;COD/m^3-day$. Reactor 2 showed somewhat better performance than Reactor 1 with COD removal efficiencies of 85.0-95.2%. The COD removal efficiencies of the reactors drastically deteriorated at OLRs above $6.2kg\;COD/m^3-day$, and the tendency was more severe for Reactor 1 than for Reactor 2. Eventhough the two reactors showed similar effluent SS concentrations at OLRs below $3.6kg\;COD/m^3-day$, Reactor 2 showed higher effluent SS concentrations than Reactor 1 at OLRs above $5.3kg\;COD/m^3-day$. Reactor 2 was stabler than Reactor 1 with a methane production rate of $5.5kg\;COD/m^3$-day at the OLR of $13.3kg\;COD/m^3-day$. An abrupt increase in effluent volatile acid concentration was observed at the OLR of $6.2kg\;COD/m^3-day$ for Reactor 1 and $7.1kg\;COD/m^3-day$ for Reactor 2. and the increase was greater in Reactor 1. In conclusion. the range of OLR for adequate treatment in the hybrid reactor was determined according to the location of the internal recirculation port. It is more desirable for higher OLRs to locate the recirculation port at the top of the packed bed in order to utilize the whole volume of the reactor.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.79-88
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• 2011

The purpose of this study was to obtain information on rates and salinity levels of irrigation for growth of Kentucky bluegrass by minimizing the hazard of salt accumulation in the sand based growing medium. Root zone profile consists of 20 cm sand based top soil, 20 cm of coarse sand as layer to interrupt capillary rise and 10 cm of reclaimed paddy soil as a base of the root zone profile. Topsoil was a mixture of dredged sand and peat with a ratio of 95%: 5% by volume. The columns were soaked into 5 cm depth saline water reservoir with salinity level of 3-5 $dSm^{-1}$. Salinity levels of irrigation water were 0, 2 and 3 $dSm^{-1}$. Irrigation rates were 3.8, 5.7 and 7.6 mm $day^{-1}$ which were equivalent to 70%, 100% and 130% of average ET (evapotranspiration) rate of Kentucky bluegrass, and irrigation interval was 3 days. Salt accumulation was due to irrigated water and moved up water from shallow water base. At the end of second year, the accumulation of salt in the rootzone showed ECc of3.86, 4.7 and 5.1 $dSm^{-1}$, and SAR of 19.2, 23.9 and 27.5 when the salinities were 0, 2 and 3 dS $m^{-1}$, respectively. Irrigation rates of 100% and 130% of ET rate with saline water did not decrease ECe and SAR in growing media. The growth of KEG was influenced by irrigation rate in the $1^{st}$ year, however, salinity level was more critical in the $2^{nd}$ year. Compared to non-saline water, saline water of 2 and 3 dS $m^{-1}$ resulted in decreased visual quality by 3.2% and 16.5%, by 6.4% and 39.3% in clipping weight, and by 5.5% and 5.0% in root mass, respectively.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.257-267
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• 1997

Fruit bodies similar to the Phellinus sp. residing on the mulberry were collected at Yang-yang in Kang-won-do province and one strain of Phellinus sp. was isolated from the fruit bodies. For mass production of the isolated mycelia in a submerged culture, the culture conditions, medium composition, and the effect of various culture systems on the mycelial growth, were investigated. The morphological characteristics of the fruit body were as follows: covered with blackish to black and rough, lower surface with yellowish-brown to dull-brown and smooth, 5-7 cm thick and hard woody. Also, the pure cultured mycelia showed yellowish-brown color, capability of purplish-brown pigment production on the PDA plate media, no-formation of clamp-connection, much binding branch, and enzyme activities such as laccase, tyrosinase and peroxidase. Therefore, pure cultured strain was identified to be Phellinus sp. In the flask culture, the optimum culture conditions for the mycelial production were obtained after cultivation of 8 days at inoculum level of 5%(v/v), media volume of 70 mL, 150 rpm, initial pH 6, and temperature of $30^{\circ}C$. Optimum medium composition from the response surface analysis were determined to be glucose 12.12 g/L, sucrose 12.12 g/L, yeast extract 11.15 g/L, malt extract 11.15 g/L, $KH_2PO_4$ 0.855 g/L and $CaCl_2$ 0.855 g/L. The production of the mycelia after 4 and 8 days of cultivation was 1.95 and 9.89 g/L, respectively. The maximum specific growth rate and productivity were $0.020\;hr^{-1}$ and 1.25 g/L/day, respectively. Among the three different culture systems for the growth of mycelia, the maximum mycelial dry weight of 7.5 g/L was obtained after cultivation of 4 days in the air-lift fermentor under aeration rate of 2.5 vvm. The maximum specific growth rate and productivity were $0.033\;hr^{-1}$ and 1.9 g/L/day, respectively, which were about 1.7 and 4.2 times higher than those of flask culture.

• Journal of the Mineralogical Society of Korea
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• v.23 no.4
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• pp.367-375
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• 2010

Understanding the interactions between earth materials and fluids is essential for studying the diverse geological processes in the Earth's surface and interior. In order to better understand the interactions between earth materials and fluids, we explore the effect of specific surface area and porosity on structural parameters of pore structures. We obtained 3D pore structures, using random packing simulations of porous media composed of single sized spheres with varying the particle size and porosity, and then we analyzed configurational entropy for 2D cross sections of porous media and cube counting fractal dimension for 3D porous networks. The results of the configurational entropy analysis show that the entropy length decreases from 0.8 to 0.2 with increasing specific surface area from 2.4 to $8.3mm^2/mm^3$, and the maximum configurational entropy increases from 0.94 to 0.99 with increasing porosity from 0.33 to 0.46. On the basis of the strong correlation between the liquid volume fraction (i.e., porosity) and configurational entropy, we suggest that elastic properties and viscosity of mantle melts can be expressed using configurational entropy. The results of the cube counting fractal dimension analysis show that cube counting fractal dimension increases with increasing porosity at constant specific surface area, and increases from 2.65 to 2.98 with increasing specific surface area from 2.4 to $8.3mm^2/mm^3$. On the basis of the strong correlation among cube counting fractal dimension, specific surface area, and porosity, we suggest that seismic wave attenuation and structural disorder in fluid-rock-melt composites can be described using cube counting fractal dimension.

• Korean Journal of Dental Materials
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• v.45 no.4
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• pp.257-274
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• 2018

The purpose of this study was to evaluate the effect of acid-treatment conditions on the surface properties of the RBM (Resorbable Blast Media) treated titanium. Disk typed cp-titanium specimens were prepared and RBM treatments was performed with calcium phosphate ceramic powder. Acid solution was mixed using HCl, $H_2SO_4$ and deionized water with 4 different volume fraction. The RBM treated titanium was acid treated with different acid solutions at 3 different temperatures and for 3 different periods. After acid-treatments, samples were cleaned with 1 % Solujet solution for 30 min and deionized water for 30 min using ultrasonic cleanser, then dried in the electrical oven ($37^{\circ}C$). Weight of samples before and after acid-treatment were measured using electric balance. Surface roughness was estimated using a confocal laser scanning microscopy, crystal phase in the surface of sample was analyzed using X-ray diffractometer. Surface morphology and components were evaluated using Scanning Electron Microscope (SEM) with Energy Dispersive X-ray spectroscopy (EDX) and X-ray Photoemission Spectroscopy (XPS). Values of the weight changes and surface roughness were statistically analyzed using Tukey-multiple comparison test (p=0.05). Weight change after acid treatments were significantly increased with increasing the concentration of $H_2SO_4$ and temperature of acid-solution. Acid-treatment conditions (concentration of $H_2SO_4$, temperature and time) did not produce consistent effects on the surface roughness, it showed the scattered results. From XRD analysis, formation of titanium hydrides in the titanium surface were observed in all specimens treated with acid-solutions. From XPS analysis, thin titanium oxide layer in the acid-treated specimens could be evaluated. Acid solution with $90^{\circ}C$ showed the strong effect on the titanium surface, it should be treated with caution to avoid the over-etching process.

• Food Science and Preservation
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• v.15 no.5
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• pp.725-730
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• 2008

The effects of acetic acid fermented juice prepared with submerged culture media of Antrodia camphorata mycelium (AJA: pH 3.2, acidity 2.0, Brix degree 3.2) on blood glucose levels and serum lipid profiles of rats in which diabetes was induced with streptozotocin (STZ) were investigated. Rats were divided into normal controls (NC), diabetic controls (DM), and groups receiving diluted (1:1, with water) AJA (A1) and undiluted AJA (A2). The volume of liquid given to both A1 and A2 animals was 0.5 mL/100 g body weight. In the A1 and A2 groups, compared with the DM group, polyphagia, liver enlargement, and weight loss caused by diabetes were considerably alleviated, but did not attain the levels of NC group rats. In the A1 and A2 groups, the levels of blood glucose were 17.1% and 28.2% lower than in the DM group. There was no significant difference in the levels of fructosamine between the DM and A1 group, but the A2 group had a level 16.3% lower than the DM group. In the A1 and A2 groups, compared with the DM group, serum triglyceride levels decreased by 44.1-48.0%, serum total cholesterol by 24.0-31.1%, and serum LDL-cholesterol by 25.2-51.1%. The level of HDL-cholesterol in A2 animals rose by 45.9% compared to NC rats. The results show that AJA may be a useful beverage for diabetes patients, offering both antihyperglycemic activity and improvement in levels of serum lipids.