• Title/Summary/Keyword: Media Introduction

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Introduction of Shiva Gene into tobacco and Potato Using Tissue-Specific Tomato PAL Promoter (조직특이성 promoter를 이용한 Shiva 유전자의 식물체내 도입)

  • 이정윤;이신우;박권우
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.2
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    • pp.109-113
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    • 1998
  • In this study we tried to transform an antimicrobial peptide gene (Shiva) under the promoter of tomato phenylalanine ammonia-lyase (tPAL5) into tobacco and potato plants. Antimicrobial peptide gene was isolated originally from giant silk moth (Hyalophora cecropia) and modified ie nucleotide sequence to increase antimicrobial activity. Transgenic tobacco plants were regenerated and their seeds were tested on the media containing kanamycin (500 mg/L). The results of PCR amplification and genomic Southern blot hybridization confirmed the integration of construct (tPAL5 promoter-Shiva-NOS-GUS-NOS) into chromosome. We observed that one of the transgenic tobacco plants showed chromosome rearrangement when integrated. In case of potato transformation, the efficiency of regeneration was maximized at the medium containing Zeatin 2mg/L, NAA 0.01mg/L, GA$_3$ 0.1mg/L. We also observed the high expression of GUS (${\beta}$-glucuronidase) enzyme which was located next to the terminator sequence of nopaline synthase gene (NOS) in the vascular tissue of stem, leaves of transgenic potatoes. This result suggested that a short sequence of Shiva gene (120 bp) and NOS terminator sequence might be served as a leader sequence of transcript when translated.

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"Poor Theatre, Poor Art" - Jerzy Grotowsky's Play and Arte Povera ('가난한 연극, 가난한 미술' - 그로토프스키 연극이론과 아르테 포베라)

  • Kang, Young-Joo
    • The Journal of Art Theory & Practice
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    • no.5
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    • pp.109-133
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    • 2007
  • What a concept of theatricality in modern art became more controversial is through a review "art and object-hood" on Michael Fried's minimal art, as having been already known broadly. As he had been concerned, the art following the minimalism is accepting as the very important elements such as the introduction of temporality, the stage in the exhibition space, and the audience's positive participation, enough to be no exaggeration to say that it was involved in almost all the theatricality. Particularly even in the installation art and the environment art, which have substantially positioned since the 1970s, the space is staged, and the audience's participation is greatly highlighted due to the temporal character and the site-specific in works. In such way, the theatricality in art work is today regarded as one of the most important elements. In this context, it is thought to have significance to examine theatricality, which is shown in the works of Arte Povera artists, who had been active energetically between 1967-1971. That is because the name of this group itself is what was borrowed from "Poor Theatre" in Jerzy Grotowski, who is a play director and theorist coming from Poland, and because of having many common points in the aspect of content and form. It reveals that the art called Arte Povera is sharing many critical minds in the face of commanding the field called a play and other media. Grotowski's theatre theory is very close to the theory and substance in Arte Povera in a sense that liberates a play, which was locked in literature, above all, renews the relationship between stage and seat and between actor and audience, and pursues a human being's change in consciousness through this. That is because Arte Povera also emphasizes the communication with the audience through appealing to a human being's perception and through the direct and living method, not the objective art concept of centering on the work. In addition, the poor play or poor art all has tendency that denies a system, which relies upon economic and cultural system, and seeks for what is anti-cultural, elemental, and fundamental. It is very similar even in a sense that focuses on the exploration process itself rather than the result, excludes the transcendental concept, and attaches importance to empiricism. However, Arte Povera accepts contradictoriness and complexity, and suggests eclecticism and tolerance, thereby being basically the nomadic art and the art difficult to be captured constitutively. On the other hand, there is difference in a sense that the poor play is characterized by purity, asceticism, seriousness, and solemnity. If so, which significance does this theatricality, which was introduced to art, ultimately have? As all the arts desire to be revealed with invisible things beyond the visual thing, theatricality comes to play a very important role at this time. If all the artists and audiences today came to acquire actual or virtual freedom much more, that can be said to be a point attributable to that art relied upon diverse conditions in a play.

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Effects of Plant Growth Regulators on Haploid Production of Wheat (밀 반수체 생산에서 식물생장조절제의 영향)

  • Kim, Young Jin;Lee, Kwang Won;Kim, Hag Sin;Cheong, Young Keun;Park, Kwang Geun;Kim, Kee Jong
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.50-55
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    • 2011
  • The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.

A Study on Power Saving Effect Through Introduction of AM Radio High Efficient Transmission System (AM 라디오 고효율전송의 전력 절감 효과 분석)

  • Lee, SangWoon
    • Journal of Broadcast Engineering
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    • v.24 no.4
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    • pp.670-675
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    • 2019
  • AM radio broadcasting has the advantage that the sound quality is lower than that of FM radio but the service area is wide and at night it can be transmitted even to overseas due to ionospheric reflection. AM radio broadcasts usually transmit large amounts of several tens to several hundreds of kilowatts (KW), requiring a lot of power, which is a financial burden on broadcasters. Recently, it is required to introduce a way to reduce the power of AM radio broadcasting in Korea. Therefore, it is possible to save a certain amount of power compared to the conventional AM radio broadcasting, and it is possible to maintain the existing license service area and sound quality, and what policies are needed to introduce the technology. In this paper, we predict the reduction of transmission power when a high efficiency transmission system is applied to existing AM radio broadcasting transmission system. As a result of the research, AMC adopting the high efficiency transmission method instead of the conventional AM radio transmission method, and when the modulation ratio of AM radio is 70%, if -3 dB companding is applied, the annual transmission power charge is decreased from 5,185,838,160Won to 3,528,900,086Won with saving of 32.0%, When -6 dB companding is applied, it is expected to be reduced to 2,608,378,934Won, which is 49.7% savings.

Vizrt Engine-Based Virtual Reality Graphics Algorithm A Study on the Basic Practical Training Method (Vizrt 엔진 기반 가상현실 그래픽 알고리즘과 기초 실습 교육 방식의 연구)

  • Cho, Hyun Kyung
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.3
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    • pp.197-202
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    • 2019
  • In the era of the fourth revolution, interest in content production using proven engines in the broadcasting sector, such as Vizrt, is growing. The new visual effects required in the 5G era are critical to content production training. Vizrt has a good production time utility and affordability for broadcast and media content. In this paper, we are going to use this to present a practical case of the theorem and application of the basic training course in the production of virtual content, and to present the basic training direction. In the introduction, the graphic algorithm analyzed and studied the characteristics and environmental factors of the Vizrt engine. In this paper, the production process was studied separately, and the work carried out through engine implementation was presented. The VS Studio Foundation was provided as a practical production case at each stage. The Vizrt engine operator process is important in graphic approach and application, and through the results of the lecture, the method of understanding and implementing algorithms for virtual reality perspective suitable for basic learning was studied. Based on practice, the research method of main theory was to create Vizrt contents specialized in 5G contents work in each sector and to implement graphic production in new areas from contents image. Through this study, we came to the conclusion of the basic training method through virtual reality content work based on Vizrt by practicing content creation according to the subject. It also proposes the effect of creating Vizrt content and the direction of building Vizrt basic training courses.

A Study on the Feasibility Analysis of AI CARE Design Graphic Planning through Modern Design Trend Analysis -Focusing on AI CARE BED part-by-part analysis and design proposal- (현대 디자인 트랜드 분석 통한 AI CARE 디자인 그래픽 기획에 관한 타당성 분석에 관한 연구 -AI CARE BED 파트별 분석과 디자인 제안을 중심으로-)

  • Cho, Hyun Kyung
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.3
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    • pp.599-604
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    • 2021
  • In an era where AI artificial intelligence converges into each field, design of AI-CARE function and UI UX design are in the spotlight. The application of shape design and the trend of color are important for visual effects to match new features. In this paper, we would like to use this to present an example of the arrangement and application of form trends and to present design directions. In the introduction, the latest design environmental factors were analyzed and studied in the direction of the new proposal. In the text, the design direction and consideration of the design in the planning were studied by separating the functional design form, and the direction of the design work was presented. At the stage of form and color, a plan was proposed to match the flow of minimalism, universal design, and affordance design. A study method based on case practice is a consideration of how to consider content on shapes and colors specialized in the partial design work, and proposed to enable graphic production of new areas of UI UX in content images to be feasible. Through this study, the design direction and planning of the derivative method of shape and color was reached by suggesting design directionality and validity for each AI CARE bed PART.

A Study on the Improvement of Services and Promotion Based on User Perception Analysis of the National Knowledge Information System (NKIS) (국가정책연구포털(NKIS)에 대한 이용자 인식 분석 기반 서비스 개선 및 홍보의 개선 방향 연구)

  • Noh, Younghee;Chang, Inho;Kang, Ji Hei;Wang, Dongho
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.32 no.4
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    • pp.25-46
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    • 2021
  • This study was conducted as a basic study to derive improvement measures for NKIS. A survey was conducted on NKIS users and the user's perception of NKIS was analyzed to derive improvement measures for NKIS. The directions for improvement presented based on the research results are as follows. First, it is necessary to survey the demand for users by occupational group and age and to establish clear targets for NKIS main users. Second, efforts to spread policy information and services are needed. Third, it is necessary to improve and revitalize the public relations method through the introduction of new media while maintaining the direction for public relations. This study presents NKIS user inconveniences and modifications through the NKIS user survey. Through this, it will increase users' NKIS utilization and contribute to the expansion of new users. In addition, it seems that it can be used as basic data when user perception surveys are conducted on similar information portals in the future. In future research, an in-depth investigation should be conducted to identify actual users of NKIS, and customized promotional measures should be established for users who actually utilize policy information to realize NKIS goals.

A Study on the Current Situation and Trend Analysis of The Elderly Healthcare Applications Using Big Data Analysis (텍스트마이닝을 활용한 노인 헬스케어 앱 사용 추이 및 동향 분석)

  • Byun, Hyun;Jeon, Sang-Wan;YI, Eun-Surk
    • Journal of the Korea Convergence Society
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    • v.13 no.5
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    • pp.313-325
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    • 2022
  • The purpose of this study is to examine the changes in the elderly healthcare app market through text mining analysis and to present basic data for activating elderly healthcare apps. Data collection was conducted on Naver, Daum, blog web, and cafe. As for the research method, text mining, TF-IDF(Term frequency-inverse document frequency), emotional analysis, and semantic network analysis were conducted using Textom and Ucinet6, which are big data analysis programs. As a result of this study, a total of six categories were finally derived: resolving the healthcare app information gap, convergence healthcare technology, diffusion media, elderly healthcare app industry, social background, and content. In conclusion, in order for elderly healthcare apps to be accepted and utilized by the elderly, they must have a good diffusion infrastructure, and the effectiveness of healthcare apps must be maximized through the active introduction of convergence technology and content development that can be easily used by the elderly.

A study about the effects of online commerce on the local retail commercial area (온라인 거래의 증가가 지역 소매 상권에 미치는 영향에 관한 연구)

  • Lee, Kangbae
    • Economic Analysis
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    • v.25 no.2
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    • pp.54-95
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    • 2019
  • The purpose of this study is to analyze quantitatively and qualitatively the effects of the increase in online shopping and its effects on real-world commercial outlets. The empirical analysis of this study is based on the results of "Census on Establishments" and "Online Shopping Survey" that cover 15 years, from 2002 to 2016. According to the results of this study, the increase in the number of online transactions affects the decrease in the number of stores in the real-world retail sector. However, non-specialized large stores and chain convenience stores showed an increase in the number of stores. In addition, the number of F&B stores increased the most in line with the increase in online transactions. This is because the increase in online transactions and in internet users led to the use of more delivery applications and the introduction of popular places on blogs or through social media. Street-level rents for medium and large-sized locations increased. In other words, it is seen that the demand for differentiated real-world stores that provide a good user experience increases, even though online transactions also increase. These results suggest that real-world stores should provide good user experiences in their physical locations with a certain size and assortment of goods.

Development of Marine Toxicity Standard Method for Marine Luminescent Bacteria: Introduction of N-Tox test (해양성 발광박테리아를 이용한 해양환경 독성평가 시험법 개발: N-Tox test)

  • Lee, Kyu-Tae;Park, Gyung-Soo;Kim, Pyoung-Joong
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.156-163
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    • 2008
  • Luminescent bacterial toxicity test was first introduced in the early 1980s, registered as international standard method in 1998 and now widely used as a common toxicity test method. This toxicity test uses luminescent bacterium, Vibrio fischeri, originated from marine environment as a test organism. The degree of toxicity can be evaluated from the comparison of luminescent emission intensity between control and treatment groups to toxicants and materials from various environmental matrix for 30 min. This test can be carried out by using commercial products and its results are sensitive and precise. This research is on the feasibility of adopting luminescent bacterial test as a domestic standard test protocol. Using commercial products, a series of experiments were conducted to identify the precision and accuracy of injection volume and light emission, and to evaluate concentration-response relationship between chemical concentrations and light emissions. Also, the feasibility of the application to environmental media and quality assurance/quality control were checked. The results of serial toxicity tests revealed that the preliminary luminescent bacterial toxicity test was robust and suitable as a standard method.