• Microbiology and Biotechnology Letters
• /
• v.27 no.2
• /
• pp.136-144
• /
• 1999

Since the condition of immobilization must be optimized, it is very important to know whether and on how conditions bacterial cells retain their metabolic activity during immobilization process. A bioluminescence intensity had the maximum value when cell concentrations were between 1.0 and 1.2 measured at O.D660. The strontium alginate was used as an immobilization matrix and two independent factors for immobilization of Photobacterium phosphoreum with strontium alginate were optimized with the response surface methodology(RSM) considering degree of bioluminescence. As a result, the optimum concentration for immobilization was found to be 2.4%(w/w) for sodium alginate and 0.31M for strontium chloride, respectively. A dilution was carried out with 2.5%(w/v) NaCl solution that is an optimum environmental condition for growth of P. phosphoreum. Under the such condition of immobilization, hardness could be predicted as 4.66$\times$104N/$m^2$ and it took different time according to the volume of matrix to be immobilized completely.

• Journal of Mechanical Science and Technology
• /
• v.15 no.1
• /
• pp.35-43
• /
• 2001

Both tensile and tear properties of short-fiber reinforced Chloroprene rubber have been studied as functions of the fiber aspect ratio and fiber content. Both properties increased when both the fiber aspect ratio and fiber content were increased. The fiber reinforced rubbers exhibited maximum values of these properties at a fiber aspect ratio of about 300. When the fiber aspect ratio exceeds 400, the mechanical properties decreased with the fiber content because of the non-uniform dispersion of fibers. The tensile modulus was compared with the prediction by the Halpin-Tsai equations for randomly oriented cases. A bonding agent was used in the fiber treating process. It was found that the ultimate tensile strength, torque, tearing energy and tensile modulus of the rubbers with treated fibers were much higher than those with untreated ones.

• KSBB Journal
• /
• v.5 no.2
• /
• pp.159-165
• /
• 1990

Ethanol fermentation by Scccharomyces cervisiae was carried out in the cell recycle filter system with a cheap fabric filter having a pore size of 10${\mu}$m. Maximum biomass concentrations up to 85g/1 were obtained, but in practice operational concentrations were between 50 and 80g/1. Ethanol productivity was 42g/1-hr, with an ethanol concentration of 66g/1 and an ethanol yield of over 86%. Continuous operation was possible by applying periodic backflushing. The ethanol fermentation could be carried out without difficulty at a dilution rate up to 0.8h-1 In order to obtain a high cell concentration and ethanol productivity, development of filter module with the larger filtration area is required.

• Journal of Institute of Control, Robotics and Systems
• /
• v.12 no.12
• /
• pp.1191-1196
• /
• 2006

In this work the kinetics of volume changes of fluid spaces associated with infusion of Ringer's solution are analyzed using the body fluid space model. During infusion of Ringer's solution, the human body is assumed to be characterized by the fluid space model into which fluid is fed and from which fluid is left. Various infusion types were tested to accommodate different medical situations. Volunteers were given Ringer's solution and the changes in blood hemoglobin were detected. From the comparison with experimental data, the single- and two-fluid space models were found to represent adequately the kinetics of human volume expansion during infusion of Ringer's solution.

• Korean Journal of Soil Science and Fertilizer
• /
• v.15 no.2
• /
• pp.141-146
• /
• 1982

Laboratory and greenhouse experments were conducted to find out the optimum dilution time of methane fermented effluent of hog waste which was high pH (7.85), $NH_4$-N (683ppm), electric conductivity (8.3mmhos/cm) and BOD (about 1,000ppm) and the interval of effluent change which will provide maximun yield of green algae (Scenedesmus accuminatus), duck weed (Lemner minor L.) and Azolla anabaena (Azolla pinnata). The results of this experiment are summerized as follows. 1. The maximum yield were obtained at 5 times diluted effluent ($NH_4$-N : 137ppm) and exchange of 4 day intertval, from 25 times to 50 times dilution ($NH_4$-N : 7-14ppm) and 4 day interval, and 50 times dilution ($NH_4$-N : 14 ppm) and exchange the effluent at 4-8 day interval for green algae, duck weed and azolla growth, respectively. 2. Purification of the effluent was enhenced by growth of the green algae, duck weed and azolla due to decrease of pH, $MH_4$-N and electric conductivity of effluent. The degree of purification was higher in case of azolla growth than duck week growth.

• KSBB Journal
• /
• v.10 no.3
• /
• pp.241-248
• /
• 1995

For the treatment of poorly biodegradable polyvinyl alcohol(PVA) in dye-processing wastewater, immobilized microbial beads were prepared by uslng agar-acrylamide method. PVA removal efficiency for the synthetic wastewater was 85% at the PVA volume loading rate of $3.1g/\ell$.day. In case of real desizing wastewater, PVA removal efficiency was 81.3% at the PVA volume loading rate of $3.25g/\ell$.day. In observation of cross section of immobilized bead passed 5 months with diameter of 2.4mm, the growth of cell was limited by the resistance of substrate and oxygen transfer for the inners region of more than 48% of bead radius from the surface. It was estimated that 70% of total removed PVA was degraded by the immobilized cells in the continuous immobilized reactor. Substrate utilization rate in the suspended reactor was decreased with increasing dilution rates above 0.083 hr-1, but that in the immobilized reactor was increased with increasing dilution rates up to 0.125hr-1. The substrate removal efficiency of immobilized reactor was much superior to that of suspended reactor with increasing dilution rates. Saturation constant of substrate utilization rate equation, Ks was $6.6 g PVA/\ell$, and maximum specific substrate utilization. k was 0.175g PVA/g cell.hr

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.4
• /
• pp.526-532
• /
• 2006

The aim of this study was to evaluate the chemical composition, in vitro DM degradability, ME and OMD of alfalfa-maize silage mixtures in comparison to pure maize and alfalfa silages, and to test the existence of associative effects of ensiling alfalfa forage with whole-crop maize using the in vitro gas production technique. Ensiling alfalfa with whole-crop maize had a significant (p<0.001) effect on chemical composition, pH, in vitro DM degradability, OMD and estimated ME values of mixtures. DM content of the resultant silages significantly increased with increasing proportion of whole-crop maize in the mixtures, whereas the pH value, crude protein (CP), acid detergent fibre (ADF) and ash contents of mixtures decreased due to the dilution effect of whole-crop maize which was low in CP, ADF and ash. The pH values of all alfalfa-maize silage mixtures were at the desired level for quality silage. Gas production of alfalfa-maize silage mixtures at all incubation times except 12 h increased with increasing proportion of whole-crop maize. When alfalfa was mixed with whole-crop maize in the ratio 40:60, ME and OMD values were significantly (p<0.001) higher than other silages. Maximum gas production ($A_{gas}$) ranged from 65.7 to 78.1 with alfalfa silage showing the lowest maximum gas production. The results obtained in this study clearly showed that maximum gas production increased with increased percentage of whole-crop maize in the silage mixtures (r = 0.940, p<0.001). It was concluded that ensiling alfalfa with whole-crop maize improved the pH, OMD and ME values. However, trials with animals are required to see how these differences in silage mixtures affect animal performance.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.5
• /
• pp.718-724
• /
• 2006

The heterogeneous association behaviour of various concentration binary mixtures of mono alkyl ethers of ethylene glycol with ethyl alcohol were investigated by dielectric measurement in benzene solutions over the entire concentration range at 25 ${^{\circ}C}$. The values of static dielectric constant $\epsilon_0$ of the mixtures were measured at 1 MHz using a four terminal dielectric liquid test fixture and precision LCR meter. The high frequency limiting dielectric constant $\epsilon_\infty$ values were determined by measurement of refractive index $n_D$ ($\epsilon_\infty\;=\;n_D\;^2$). The measured values of $\epsilon_0$ and $\epsilon_\infty$ were used to evaluate the values of excess dielectric constant $\epsilon^E$, effective Kirkwood correlation factor $g^{eff}$ and corrective correlation factor $g_f$ of the binary polar mixtures to obtain qualitative and quantitative information about the H-bond complex formation. The non-linear behaviour of the observed $\epsilon_0$ values of the polar molecules and their mixtures in benzene solvent confirms the variation in the associated structures with change in polar mixture constituents concentration and also by dilution in non-polar solvents. Appearance of the maximum in $\epsilon^E$ values at different concentration of the polar mixtures suggest the formation of stable adduct complex, which depends on the molecular size of the mono alkyl ethers of ethylene glycol. Further, the observed $\epsilon^E$ < 0 also confirms the heterogeneous H-bond complex formation reduces the effective number of dipoles in these polar binary mixtures. In benzene solutions these polar molecules shows the maximum reduce in effective number of dipoles at 50 percent dilutions. But ethyl alcohol rich binary polar mixtures in benzene solvent show the maximum reduce in effective number of dipoles in benzene rich solutions.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.7
• /
• pp.1221-1225
• /
• 2007

A ${\beta}$-ionone-resistant mutant strain isolated from the red yeast Xanthophyllomyces dendrorhous KCTC 7704 was used for batch and continuous fermentation kinetic studies with glucose media in a 2.5-1 jar fermentor at $22^{\circ}C$ and pH 4.5. The kinetic pattern of growth and carotenoid concentration in the batch fermentations exhibited a so-called mixed-growth-associated product formation, possibly due to the fact that the content of intracellular carotenoids depends on the degree of physical maturation toward adulthood. To determine the maximum specific growth rate constant (${\mu}_m$) and Monod constant ($K_s$) for the mutant, glucose-limited continuous culture studies were performed at different dilution rates within a range of $0.02-0.10\;h^{-1}$. A reciprocal plot of the steady-state data (viz., reciprocal of glucose concentration versus residence time) obtained from continuous culture experiments was used to estimate a ${\mu}_m$ of $0.15\;h^{-1}$ and $k_s$ of 1.19 g/l. The carotenoid content related to the residence time appeared to assume a typical form of saturation kinetics. The maximum carotenoid content ($X_m$) for the mutant was estimated to be $1.04\;{\mu}g/mg$ dry cell weight, and the Lee constant ($k_m$), which was tentatively defined in this work, was found to be 3.0 h.

• Korean Journal of Environmental Agriculture
• /
• v.42 no.2
• /
• pp.93-103
• /
• 2023

Persistence and degradation patterns of acequinocyl and its metabolite, hydroxyl-acequinocyl (acequinocyl-OH) and fenpyroximate in butterburs (Petasites japonicus Max.) were investigated after pesticide application. Butterburs, one of the minor crops in South Korea, was planted in two plots (plot A for double and plot B for single application) in a greenhouse. Butterburs samples were also planted in a separate plot without pesticide treatment, as the control. A commercial pesticide containing acequinocyl and fenpyroximate was applied to the foliage of butterburs at hourly intervals after dilution. Recoveries of acequinocyl and acequinocyl-OH were 78.6-84.7% and 83.7-95.5%, respectively; the relative standard deviation of the two compounds were less than 5%. The method limit of quantification was 0.01 mg/kg. The total (Ʃ) acequinocyl residues in butterburs reduced by 96.0% at 14 days and 75.9% at 7 days, in plot A and B, respectively, after final pesticide applications. The biological half-life (DT₅₀) of Ʃ acequinocyl and fenpyroximate, calculated using the dissipation rate, was 3.0 days and 4.0 days, respectively. These data were used to set up maximum residue and safe standard levels when the pesticides are applied to control pests during butterbur cultivation. Risk assessment results showed that the maximum % acceptable daily intake was 7.74% for Ʃ acequinocyl and 0.16% for Ʃ fenpyroximate. The theoretical maximum daily intake of Ʃ acequinocyl and fenpyroximate was 26.3% and 35.8%, respectively. In conclusion, the concentrations of Ʃ acequinocyl and fenpyroximate in butterburs pose no significant health risks to Koreans.