• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.244-249
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• 2004

There have been many attempts to predict the secondary structure content of a protein from its primary sequence, which serves as the first step in a series of bioinformatics processes to gain knowledge of the structure and function of a protein. Most of them assumed that prediction relying on the information of the amino acid composition of a protein can be successful. Several approaches expanded the amount of information by including the pair amino acid composition of two adjacent residues. Recent methods achieved a remarkable improvement in prediction accuracy by using this expanded composition information. The overall average errors of two successful methods were 6.1% and 3.4%. This work was motivated by the observation that evolutionarily related proteins share the similar structure. After manipulating the values of the frequency matrix obtained by running PSI-BLAST, inputs of an artificial neural network were constructed by taking the ratio of the amino acid composition of the evolutionarily related proteins with a query protein to the background probability. Although we did not utilize the expanded composition information of amino acid pairs, we obtained the comparable accuracy, with the overall average error being 3.6%.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.45-45
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• 2021

This study aimed to explore research on bibliometric features of cannabis by applying scientometric analysis method, and to approach experimental research evaluation based on it. A total of 30,352 articles on cannabis published since 2001 from SCOPUS were analyzed using KnowledgeMatrix Plus and VOSviewer software. Results showed differences in research activities in countries where cannabis is legalized (Canada, the United States, the Netherlands) and Asian countries where its use is illegal. Related to medical cannabis, there has been a noticeable increase in the number of studies on pain, epilepsy, seizures and brain diseases such as multiple sclerosis. In the field of basic research, the number of pharmacological studies of cannabis on the cannabinoid type 1 receptor signaling pathway and inflammation and obesity has increased significantly. Subsequent experimental studies have compared the anti-inflammatory effects of four parts of Korean cannabis such as root, stem, leaf, and bark. Consistent with the predicted results of the scientometric analysis, all parts of C. sativa showed inhibitory effects on COX-2, NO/iNOS and TNF-α expression in LPS-stimulated RAW264.7 cells. These attempts provide an experimental research approach based on scientometric assessment.

• Mass Spectrometry Letters
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• v.11 no.4
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• pp.118-124
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• 2020

An analytical method was developed for hypoxia-inducible factor (HIF) stabilizers based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation and liquid chromatography-high resolution mass spectrometry analysis. HIF stabilizers potentially enhance the performance of athletes, and hence, they have been prohibited. However, the analysis of urinary HIF stabilizers is not easy owing to their unique structure and characteristics. Hence, we developed the QuEChERS preparation technique for a complementary method and optimized the pH, volume of extraction solvent, and number of extractions. We found that double extraction with 1% of formic acid in acetonitrile provided the highest recovery of HIF stabilizers. Moreover, the composition of the mobile phase was also optimized for better separation of molidustat and IOX4. The developed method was validated in terms of its precision, detection limit, matrix effect, and recovery for ISO accreditation. To the best of our knowledge, this is the first demonstration of the application of the QuEChERS method, which is suitable as a complementary analytical method, in antidoping.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.1013-1017
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• 2008

Syndecan-3 is a cell-surface heparan sulfate proteoglycan, which performs a variety of functions during cell adhension process. It is also a coreceptor for growth factor, mediating cell-cell and cell-matrix interaction. Syndecan-3 contains a cytoplasmic domain potentially associated with the cytoskeleton. Syndecan-3 is specifically expressed in neuron cell and has related to neuron cell differentiation and development of actin filament in cell migration. Syndecans each have a unique, central, and variable (V) region in their cytoplasmic domains. And that region of syndecan-3 may modulate the interactions of the conserved C1 regions of the cytoplasmic domains by tyrosine phosphorylation. Cytoplasmic domain of syndecan-3 has been synthesized for NMR structural studies. The solution structure of syndecan-3 cytoplasmic domain has been determined by two-dimensional NMR spectroscopy and simulated-annealing calculation. The cytoplasmic domain of the syndecan proteins has a tendency to form a dimmer conformation with a central cavity, however, that of syndecan-3 demonstrated a monomer conformation with a flexible region near C-terminus. The structural information might add knowledge about the structure-function relationships among syndecan proteins.

• BMB Reports
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• v.43 no.5
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• pp.305-310
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• 2010

Syndecans, cell surface heparansulfate proteoglycans, have been proposed to act as cell surface receptors and/or coreceptors to play critical roles in multiple cellular functions. However, recent reports suggest that the function of syndecans can be further extended through shedding, a cleavage of extracellular domain. Shedding constitutes an additional level for controlling the function of syndecans, providing a means to attenuate and/or regulate amplitude and duration of syndecan signals by modulating the activity of syndecans as cell surface receptors. Whether these remaining cleavage products are still capable of functioning as cell surface receptors to efficiently transduce signals inside of cells is not clear. However, shedding transforms cell surface receptor syndecans into soluble forms, which, like growth factors, may act as novel ligands to induce cellular responses by association with other cell surface receptors. It is becoming interestingly evident that shed syndecans also contribute significantly to syndecan functions in cancer biology. This review presents current knowledge about syndecan shedding and its functional significance, particularly in the context of cancer.

• The Journal of the Acoustical Society of Korea
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• v.39 no.3
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• pp.191-199
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• 2020

Currently, studies on the estimation algorithm based on compressive sensing are actively underway, but to the best of our knowledge, no study on the performance of the Sparse Spectrum Fitting (SpSF) algorithm in nonuniform sensor arrays has been made. This paper deals with the derivation of the compressive sensing based covariance fitting algorithm extended to the frequency domain. In addition, it shows the performance of directon-of-arrival estimation of the frequency domain SpSF algorithm in non-uniform linear sensor array system and the sensor array failure situation.

• Journal of Korean Academy of Nursing
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• v.48 no.4
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• pp.419-431
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• 2018

Purpose: The purpose of this study was to develop and establish the psychometric properties of a clinical nursing competency evaluation tool to be utilized by clinical preceptors. Methods: The initial items were identified through in-depth literature review and field interviews based on a hybrid model. Content validation of the items was evaluated through three rounds of content validity testing. Participants were 34 clinical preceptors and 443 nursing students participating in clinical practice. Data were analyzed using exploratory and confirmatory factor analysis, convergence and discriminant validity, internal consistency and inter-rater reliability. Results: The final scale consisted of 23 items and four factors, fundamental nursing skills performance, critical thinking skills based on the nursing process, basic nursing knowledge, and professional attitude; these factor explained 69.7% of the total variance. The analysis with multi-trait/multi-item matrix correlation coefficients yielded 100.0% and 95.7 % convergence and discriminant validity, respectively. Cronbach's alpha for the total items was .95. The four subscale model tested by confirmatory factor analysis was satisfactory. Inter-rater reliability ranged from .912 to .967. Conclusion: This scale was found to be a reliable and valid instrument that clinical preceptors can apply for evaluating the clinical nursing competency of nursing students in clinical settings.

• Journal of the Korean housing association
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• v.8 no.3
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• pp.153-162
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• 1997

Personalities. human relations. living patterns. and so on of the aged have been moulded respectively for a long period of time and their present situation of health. family make-up. economic condition etc. are different individually. Various characteristics - physiological. psychological and social peculiarities - of the aged are analyzed and studied in preceding paper to grope planning factors of architectural spaces by referring to sundry records from many branch gerontology, medical science. psycology. sociology. etc. - of knowledge in a position of architectural planning. So. in this paper. the directions of planning residential environment for the aged are studied on the base of those planning factors of architectural spaces. there are room(X$_1$). unit home(X$_2$), public housing(X$_3$), residential district(X$_4$) as dwelling spaces for the aged in X axis and safety(Y$_1$). hygiene(Y$_2$). convenience (Y$_3$), comfortableness (Y$_4$). psychology ($Y^5$), aesthetics ($Y^6$). society ($Y^7$). economy ($Y^8$) as the terms desired to spaces in Y axis. And the directions of planning residential environment for the elderly are studied by discussing mutual matters relevant to the subject on the cells of matrix formed by X. Y axis. In planning residential environment for the elderly. their physiological. psychological. social characteristics should be considered thoroughly and many types of dwelling spaces should be built. Then they can take up their abode to suit their conditions easily.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.11a
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• pp.308-312
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• 2003

The packaging of the integrated circuits requires knowledge of ceramics and metals to accommodate the fabrication of modules that are used to construct subsystems and entire systems from extremely small components. Composite ceramics ($Al_2O_3-SiO_2$) were tested for substrates. A stress analysis was conducted for a linear work-hardening metal cylinder embedded in an infinite ceramic matrix. The bond between the metal and ceramic was established at high temperature and stresses developed during cooling to room temperature. The calculations showed that the stresses depend on the mismatch in thermal expansion, the elastic properties, and the yield strength and work hardening rate of the metal. Experimental measurements of the surface stresses have also been made on a $Cu/Al_2O_3-SiO_2$ ceramic system, using an indentation technique. A comparison revealed that the calculated stresses were appreciably larger than the measured surface stresses, indicating an important difference between the bulk and surface residual stresses. However, it was also shown that porosity in the metal could plastically expand and permit substantial dilatational relaxation of the residual stresses. Conversely it was noted that pore clusters were capable of initiating ductile rupture, by means of a plastic instability, in the presence of appreciable tri-axiality. The role of ceramics for packaging of microelectronics will continue to be extremely challenging.

• Industrial Engineering and Management Systems
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• v.14 no.1
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• pp.58-72
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• 2015

A primary concern of national R&D plans is to encourage technological development in private firms and research institutes. For effective R&D planning and program support, it is necessary to assess technological impacts that may exist both directly and indirectly among technology areas within the whole technology system; however, previous studies analyze only direct impacts among technologies, failing to capture both direct and indirect impacts. Therefore, this study proposes an approach based on decision-making trial and evaluation laboratory (DEMATEL) to identifying specific characteristics of technology areas, such as technological impact and degree of cause or effect (DCE). The method employs patent co-classification analysis to construct a technological knowledge flow matrix. Next, to capture both direct and indirect effects among technology areas, it incorporates the modified DEMATEL process into patent analysis. The method helps analysts assess the technological impact and DCE of technology areas, and observe their evolving trajectories over time, thereby identifying relevant technological implications. This study presents a case study using Korean patents registered during 2003-2012. We expect our analysis results to be helpful input for R&D planning, as well as the suggested approach to be incorporated into processes for formulating national R&D plans.