• Korean Journal of Pharmacognosy
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• v.31 no.4
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• pp.421-425
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• 2000

HPLC method was applied to the quantitative analysis of lupine alkaloid, matrine from alkaloid fraction of Sophorae Radix (Sophora flavescens). The average content of matrine from 20 Sophorae Radices showed $0.13{\pm}0.06%$.

• Biomolecules & Therapeutics
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• v.25 no.4
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• pp.390-395
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• 2017

The present study investigated the sedative effects of Sophora flavescens (SF) and its bioactive compound, matrine through performing locomotor activity test and the electroencephalography (EEG) analysis in the rat. The underlying neural mechanism of their beneficial effects was determined by assessing c-Fos immunoreactivity and serotonin (5-HT) in the brain utilizing immunohistochemical method and enzyme-linked immunosorbent assay. The results showed that SF and matrine administration had an effect on normalization of caffeine-induced hyperactivity and promoting a shift toward non-rapid eye movement (NREM) sleep. c-Fos-immunoreactivity and 5-HT level in the ventrolateral preoptic nucleus (VLPO), a sleep promoting region, were increased in the both SF and matrine-injected groups. In conclusion, SF and its bioactive compound, matrine alleviated caffeine-induced hyperactivity and promoted NREM sleep by activating VLPO neurons and modulating serotonergic transmission. It is suggested that SF might be a useful natural alternatives for hypnotic medicine.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.244-248
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• 2013

This study was carried out to investigate the residual characteristics and calculate processing factor of the environment friendly material matrine in fresh chilli pepper by drying. Spray solution of matrine was prepared by dilution of the commercial product (2% active ingredient) with water at 1 : 1000 (v/v) ratio and sprayed onto chilli pepper plants at seven day intervals. Samples were collected at 0, 1, 3, 5 and 7 days after last application and then dried using a hot air dry oven at $60^{\circ}C$ for 36 hours until the water content was reduced to 14%. Recoveries and storage period stabilities of matrine in the samples ranged from 106.6 to 119.1% and 106.6 to 113.1%, respectively. The residual concentrations of matrine in fresh chilli pepper and dried chilli peppers treated only once were found to be from less than 0.01 to 0.11 and from 0.03 to 0.25 mg/kg, respectively. In case of plants sprayed twice with matrine, the residual concentrations ranged from 0.02 to 0.12 and from 0.04 to 0.4 mg/kg, respectively. Processing factor of matrine in the fresh chilli pepper by drying was found to be from 1.5 to 3.3, indicating that the residual concentration of matrine in dried chilli pepper increased about two or three times by drying.

• The Korean Journal of Pesticide Science
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• v.17 no.3
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• pp.213-219
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• 2013

This study focused on the promotion of the insecticidal activity of eco-friendly insecticidal materials against four major insect pest in the organic Chinese cabbage cultivation area. Among insecticidal materials mixture, BT+Matrine+Neem against leaf beetle larva, Phaedon brassicae, showed the highest 94.1% at 0.05% which is the lowest concentration of three treated concentrations. The insecticidal activity of Matrine, Neem, and BT alone (0.1%, w/v) or the mixtures (0.05%, w/v) against Cabbage worm larva, Artogeria rapae, was investigated by leaf disc spray method. The insecticidal efficacy of the mixtures of two or three eco-friendly materials was higher than the single treatment of each material with 94.3%, 100% and 100%. Control efficacy of 0.3% COY+0.05% (95.3%) against the green peach aphid, Myzus persicae was higher than that of 0.3% COY+0.05% Neem (90.2%) and lasted for more than 21 days after one time treatment. At 35 days after treatment, the effect of 0.05% BT+0.05% Matrine and 0.05% BT+0.05% Matrine+0.05% Neem treatments against diamondback moth showed very high insecticidal activity with more than 90% of control value. Therefore, BT, Neem, and Matrine where are properly treated could be effective eco-friendly materials for controlling major insect pests in an organic Chinese cabbage field.

• Mycobiology
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• v.45 no.2
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• pp.90-96
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• 2017

Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and $NH_4NO_3$ was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at $20.67{\mu}g/L$, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and $NH_4NO_3$ was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of $20.63{\pm}0.11{\mu}g/L$, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.

• YAKHAK HOEJI
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• v.59 no.3
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• pp.92-97
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• 2015

KIOM-MA128 is a novel Korean herbal medicine with anti-atopic, anti-inflammatory and anti-asthmatic effects. This article presents the first pharmacokinetic study on KIOM-MA128. The purpose of this study was to characterize a pharmacokinetic characteristic of matrine, a potential marker of KIOM-MA128, in rats using population pharmacokinetic model. 1, 2 and 8 g/kg of KIOM-MA128 were administered to rats orally and plasma concentrations of matrine was determined by HPLC-MS/MS. Non-compartmental analysis (NCA) was performed using Phoenix$^{(R)}$ and pharmacokinetic model was built using NONMEM$^{(R)}$. This model was validated with internal validation which is visual predictive check (VPC) and bootstrap. The NCA result of matrine showed that $C_{max}$ was 294.24, 552.22 and 868.65 ng/ml, $AUC_{inf}$ was 1273.05, 2724.76 and $9743.25ng{\cdot}hr/ml$ and $T_{max}$ was 1, 1.3 and 2.3 hr for the doses of 1, 2, and 8 g/kg, respectively. The rat plasma concentrations were described very well with one-compartment model. Pharmacokinetic model for matrine was successfully developed and evaluated. Finally, our model is helpful to understand pharmacokinetic characteristic of KIOM-MA128.

• The Korean Journal of Pesticide Science
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• v.19 no.4
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• pp.411-417
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• 2015

This study is conduced to investigate control efficacy of mixture of plant extracts which has been reported to control pest and Bacillus thuringiensis or alone. Control efficacy of 0.025% matrine or mixture of 0.016% matrine and BT ($5{\times}10^4cfu$) against $3^{rd}$ instar larvae of beet armyworm were best as 98.7% and 93.3%. Treatment of mixture of 0.01% matrine and BT ($5{\times}10^4cfu$) showed the mortality of 69.3%. In the case of neem, when the mixture of 0.1% neem and BT was applied to larvae of S. exigua, their mortality was 50.0% and weight of larvae were significantly different compare with control and only neem treatment. Therefore For effective control reducing population of beet armyworm and possibility to develop resistance against BT and eco-friendly agents, mixture of 0.016% Matrine and BT ($5{\times}10^4cfu$) could be used.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.5
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• pp.2169-2177
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• 2014

Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and timedependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.spc1
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• pp.155-161
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• 2004

• Korean journal of applied entomology
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• v.62 no.4
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• pp.267-275
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• 2023

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of insecticidal active ingredients in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of active ingredients, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan-Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.