• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1371-1377
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• 2002

During a 5-yr period, 1994-1998, pre-weaning and weaning data were collected on 591 calves produced by mating either straightbred Jersey, straightbred Limousin or $F_1$ (Limousin${\times}$Jersey) bulls to mature purebred Jersey or Limousin cows. Traits recorded included birth and weaning weight, height, length, girth, fat depth and a measure of muscle (ratio of stifle to hip width expressed as a percentage). All traits were analyzed assuming a model with sire and dam random effects that included effects of year and date of birth, sex, breed and year${\times}$sex interaction. Main effects were generally significant with few exceptions. Direct genetic effects were large for weight, height, girth and muscle with a breed trend from purebred Jersey (small) to purebred Limousin (large). At weaning, the maternal effect of the Jersey dam was positive for weight (10.9${\pm}$4.9 kg), girth (3.7${\pm}$1.0 cm) and muscle (6.0${\pm}$0.9%). Heterosis was highly significant and positive only for fat depth (1.5${\pm}$0.2 mm) with the $F_1$ progeny being the fattest, followed by the backcrosses, then purebred Jersey and purebred Limousin. Also, significant (p<0.001) but negative heterosis was observed for weight, girth and muscle. The change in ranking for fat depth relative to other traits is a reflection of the large heterotic effects relative to direct effects on fat depth. Epistatic effects were not significant on any trait at birth or weaning. This study has indicated the possibility of exploiting the positive heterotic and maternal effects for fat depth and muscularity to meet specific meat quality and quantity demand by consumers.

• Animal cells and systems
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• 제1권2호
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• pp.247-257
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• 1997

Genetic and morphological variations of Korean Hynobius leechii were surveyed. Starch gel electrophoresis was used to study genetic variation at 23 loci. H. leechii is genetically divided into three groups, each of which is named as Form-A, Form-B, and Form-C. These forms are clearly separated with 4-5 fixed or nearly fixed allelic differences. At a sympatry near Jangseong, Jollanam-do, a significant deviation from random mating between Form-A and Form-B was observed. Therefore, they are considered as two distinct taxi of specific rank. Form-A is widely distributed in the inland of Korea, whereas Form-B is distributed in the islands or near the coastal regions of Western and Southern Korea. Form-B had, in addition, a significant morphological difference from Form-A. On the other hand, Form-C was restricted to an area near Kori atomic power plant, Kyongsangnam-do, and the genetic similarities between this form and Form-A and Form-B were 0.742 and 0.736, respectively. Moreover, Form-C had a unique color pattern, short body and small coccyx. Thus, it seems reasonable to assign this form as another new species.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.20-20
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• 2003

We described the isolation, characterization and inheritance of twenty-seven microsatellite loci from olive flounder, Paralichthys olivaceus. All loci were found to be polymorphic, and had between five and 22 alleles with observed heterozygosity ranging from 0.161 to 1.0 in 31 individuals examined. Allele segregation patterns of all loci in controlled crosses of flounder were studied to assess the inheritability. Allele of all but 3 loci were segregated according to Mendelian transmission. However, 3 loci had a possibility of scoring errors of heterozygous individuals caused by unreproducible PCR amplification of a particular allele. 24 microsatellite loci are likely to be useful for studies of genome mapping, mating systems and population genetics in this species.

• Journal of Forest and Environmental Science
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• 제28권1호
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• pp.1-7
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• 2012

Tropical forests whether fragmented or undisturbed or be they equatorial or deciduous, remain the storehouse of biodiversity for hundreds of thousands of plant and animal species. This unique characteristic continues to attract a wide range of scientists and international organizations to study and attempt to understand tropical forest ecosystems. Gene flow is mediated by pollen, seed and seedling dispersal, and factors affecting this gene flow include phenology, spatial distribution, population structures, seed predation, sexual and mating systems as well as physical and biological barriers to gene flow. Two methods are used in measuring gene flow: direct method that relies on the actual observation of seed and pollen dispersal, whereas indirect methods involve the use of genetic markers such as allozymes and DNA techniques. Political strife, extreme natural and artificial disasters, the lack of a comprehensive forestry research vision, coupled with difficult socio-economic conditions in Africa have made the environment quite difficult for sustained research activities on the part of those undertaking or wishing to undertake such studies. Gene flow studies in this region are few and far between. This review elaborates on the mechanisms of gene flow mediation in Sub-Saharan Africa.

• 한국균학회지
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• 제9권1호
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• pp.13-18
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• 1981

1. 느타리버섯 농기(農技) 201호(號), 기존보급품종(旣存普及品種), 사철느타리버섯은 사극성(四極性) 교배형식(交配形式)을 가지고 있으며 각(各) 균주간(菌株間)에 는 교배(交配)가 가능(可能)하였다. 2. 균주간(菌株間) 교배균주(交配菌株)는 균주내(街株內) 교배종주(交配種株)보다 균사생장(菌絲生長)이 빠르고 자실체(子實體) 수량(收量)도 높았다. 3. 다수성(多收性)인 느타러버섯 농기(農技) 201호(號) 및 사철느타리버섯의 1핵(核) 균사체(菌絲體)를 교배(交配)할 때 자실체(子寶體) 수량(收量)이 가장 높았다.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.237-244
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• 1994

The vector systems for the expression and secretion of human lipocortin-l (LC1) from Saccharomyces cerevisiae were constructed with GAL10 promoter and the prepro leader sequence of mating factor-$\alpha$1. They were further constructed to contain three different transcription terminators; GAL7 terminator, LCl terminator and a fused form of these two terminators. The expression and secretion levels of LCl were compared to investigate the effect of transcription terminators on the LCl gene expression. For the expression cassettes employing the GAL7 terminator or the terminator of fused form, the expression levels of LCl were measured by scanning the immunoreactive LCl protein bands, and were found to be 0.27 g/l and 0.32 g/l, respectively. The highest expression level of 0.54 g/l was obtained with the expression vector containing the LCl transcription terminator. In all expression cassettes, the majority of LCl proteins expressed were retained intracellularly, indicating a low secretion efficiency of about 5%. The high expression level of LCl was explained by the great content and stability of LCl mRNA transcribed from the LCl terminator-employing vector. The results of this study demonstrate that the LCl transcription terminator functions for the expression of LCl in S. cerevisiae better than the GAL7 terminator.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.179-183
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• 1999

A recombinant form of hirudin, a potent thrombin-specific inhibitor derived from the bloodsucking leech, was expressed as a secretory product in Saccharomyces cerevisiae under the control of GALl0 promoter and the mating factor $\alpha$pre-pro leader sequence. In an attempt to produce recombinant hirudin (r-Hir) of therapeutic purity in large quantities, the fed-batch fermentation was carried out by using this recombinant yeast, and subsequently downstream processing was developed with the preparative-scale column chromatography systems. About 234 mg/l of biologically active r-Hir was produced as a secretory product by the fed-batch fermentation strategy developed for an efficient downstream processing. Using a two-step chromatography process (an anion exchange chromatography followed by the reverse phase HPLC), the r-Hir was purified to>98% with an overall recovery yield of 84%. According to the N-terminal amino acid sequencing, the purified r-Hir was found to have the predicted N-terminal amino acid sequence. The biological activity of the purified r-Hir to inhibit thrombin was also identical to that of the commercial hirudin.

• 한국CDE학회논문집
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• 제10권2호
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• pp.133-142
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• 2005

This paper proposes an object-oriented conceptual information model of mechanical assemblies, named open assembly model (OAM). The proposed assembly model primarily defines hierarchical relationships between parts and subassemblies. Together with the assembly hierarchy. the model also provides a way to represent tolerances, kinematic information, and parametric assembly constraints. Relational information such as mating conditions and degree of freedom between parts and subassemblies is captured via assembly features and relationships thereof. The information model is described using class diagrams of the Unified Modeling Language (UML), and instance diagrams are used to exemplify the proposed information model. The conceptual model presented in this paper is an integrated information model for assembly representation, which could supply necessary information for tolerance analysis and synthesis, kinematic simulation, and assembly simulation. Such a conceptual information model plays an important role for the exchange of information between modeling, analysis and planning systems. Hence, the proposed model could serve as a framework for developing data exchange standards of mechanical assemblies. The proposed model is demonstrated through a case study of a planetary gear assembly.

• Animal cells and systems
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• 제4권2호
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• pp.109-115
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• 2000

To clarify taxonomic status of the two sibling species, Moroco oxycephalus and M. lagowskii reproductive isolation mechanisms were investigated at sympatric area located in Kansung-up, Kosung-gun, Kangwon-do, Korea. Genetic analysis was performed to reveal mating system and intensity of Hybridization between the two species. The frequencies of hybrids were increased since 1989, and then the observed hybrid frequencies ($H_O$) did not significantly differ from the expected hybrid ($H_E$) in 1998 and 1999. However, based on histological analysis of two parents and their hybrid s gonads, the hybridizations between M. oxycephalus and M. lagowskii produced mostly fertile females but sterile males in accordance with Haldane s rule. Although it was suspected that pre- and postmating isolation mechanisms were affected between the two species, M. oxycephalus and M. lagowskii seemed to be strongly isolated with microhabitat at sympatry until 1997. Since 1998, hybrid frequencies were increased by habitat disturbance. However, their hybrid frequencies would be reduced by postmating isolation mechanisms. Therefore, the two species are considered to be distinct species recently diverged.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권9호
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• pp.2976-2990
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• 2022

The adaptive adjustment of reference or weight vectors in decomposition-based methods has been a hot research topic in the evolutionary community over the past few years. Although various methods have been proposed regarding this issue, most of them aim to diversify solutions in the objective space to cover the true Pareto fronts as much as possible. Different from them, this paper proposes a knee point-based reference vector adaptive adjustment strategy to concurrently balance the convergence and diversity. To be specific, the knee point-based reference vector adaptive adjustment strategy firstly utilizes knee points to construct the adaptive reference vectors. After that, a new fitness function is defined mathematically. Then, this paper further designs a many-objective evolutionary algorithm with knee point-based reference vector adaptive adjustment strategy, where the mating operation and environmental selection are designed accordingly. The proposed method is extensively tested on the WFG test suite with 8, 10 and 12 objectives and MPDMP with state-of-the-art optimizers. Extensive experimental results demonstrate the superiority of the proposed method over state-of-the-art optimizers and the practicability of the proposed method in tackling practical many-objective optimization problems.