• Title/Summary/Keyword: Massey

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Effects of Feeding Condensed Tannin-containing Plants on Natural Coccidian Infection in Goats

  • Hur, Sam N.;Molan, Abdul L.;Cha, Jang O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.9
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    • pp.1262-1266
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    • 2005
  • Twelve Korean native goats, spontaneously infected with mixed species of Eimeria were used to study the possible direct anticoccidial effect of feeding condensed tannin-containing plants on the production of Eimeria oocysts. The effects of feeding pine (Pinus densifora) needles, oak (Quercus acutissima) leaves and lucerne chaff on coccidia oocyst output were studied for a period of 10 days post-feeding. The results indicate that feeding fresh pine needles (40 g condensed tannins (CT) dry matter (DM)/day/goat) and oak leaves (40 g CT DM/day/goat) in combination with lucerne chaff had rapid anticoccidial activities in goats as demonstrated by a sharp decrease in oocyst production. Two days after feeding, the numbers of oocysts per gram of faeces (OPG) from the goats fed pine needles with lucerne chaff, and from goats fed oak leaves reduced by 40% and 44% compared to pre-feeding, respectively. On the sixth day after commencing feeding pine needles and oak leaves, the reduction was 81% and 72%, respectively. Ten days after feeding pine needles and oak leaves, the OPG was reduced by 93% and 85%, respectively compared to pre-feeding. Statistical analysis showed that feeding pine needles and oak leaves to goats naturally infected with coccidia significantly (p<0.001) reduced the numbers of oocysts compared to the control group fed lucerne chaff only. Four clinically important species of coccidia, Eimeria parva, Eimeria ninakohlyakimovae, Eimeria christenseni and Eimeria arloingi were identified in Korean native goats.

Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway

  • Jung, Tae-Hwan;Park, Jeong Hyeon;Jeon, Woo-Min;Han, Kyoung-Sik
    • Nutrition Research and Practice
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    • v.9 no.4
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    • pp.343-349
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    • 2015
  • BACKGROUND/OBJECTIVES: Fermentation of dietary fiber results in production of various short chain fatty acids in the colon. In particular, butyrate is reported to regulate the physical and functional integrity of the normal colonic mucosa by altering mucin gene expression or the number of goblet cells. The objective of this study was to investigate whether butyrate modulates mucin secretion in LS174T human colorectal cells, thereby influencing the adhesion of probiotics such as Lactobacillus and Bifidobacterium strains and subsequently inhibiting pathogenic bacteria such as E. coli. In addition, possible signaling pathways involved in mucin gene regulation induced by butyrate treatment were also investigated. MATERIALS/METHODS: Mucin protein content assay and periodic acid-Schiff (PAS) staining were performed in LS174T cells treated with butyrate at various concentrations. Effects of butyrate on the ability of probiotics to adhere to LS174T cells and their competition with E. coli strains were examined. Real time polymerase chain reaction for mucin gene expression and Taqman array 96-well fast plate-based pathway analysis were performed on butyrate-treated LS174T cells. RESULTS: Treatment with butyrate resulted in a dose-dependent increase in mucin protein contents in LS174T cells with peak effects at 6 or 9 mM, which was further confirmed by PAS staining. Increase in mucin protein contents resulted in elevated adherence of probiotics, which subsequently reduced the adherent ability of E. coli. Treatment with butyrate also increased transcriptional levels of MUC3, MUC4, and MUC12, which was accompanied by higher gene expressions of signaling kinases and transcription factors involved in mitogen-activated protein kinase (MAPK) signaling pathways. CONCLUSIONS: Based on our results, butyrate is an effective regulator of modulation of mucin protein production at the transcriptional and translational levels, resulting in changes in the adherence of gut microflora. Butyrate potentially stimulates the MAPK signaling pathway in intestinal cells, which is positively correlated with gut defense.

New Low-Power and Small-Area Reed-Solomon Decoder (새로운 저전력 및 저면적 리드-솔로몬 복호기)

  • Baek, Jae-Hyun;SunWoo, Myung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.6
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    • pp.96-103
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    • 2008
  • This paper proposes a new low-power and small-area Reed-Solomon decoder. The proposed Reed-Solomon decoder using a novel simplified form of the modified Euclid's algorithm can support low-hardware complexity and low-Power consumption for Reed-Solomon decoding. The simplified modified Euclid's algorithm uses new initial conditions and polynomial computations to reduce hardware complexity, and thus, the implemented architecture consisting of 3r basic cells has the lowest hardware complexity compared with existing modified Euclid's and Berlekamp-Massey architectures. The Reed-Solomon decoder has been synthesized using the $0.18{\mu}m$ Samsung standard cell library and operates at 370MHz and its data rate supports up to 2.9Gbps. For the (255, 239, 8) RS code, the gate counts of the simplified modified Euclid's architecture and the whole decoder excluding FIFO memory are only 20,166 and 40,136, respectively. Therefore, the proposed decoder can reduce the total gate count at least 5% compared with the conventional DCME decoder.

Water-insoluble, Whey Protein-based Microcapsules for Controlled Core Release Application

  • Lee, Sung-Je
    • Journal of Dairy Science and Biotechnology
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    • v.23 no.2
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    • pp.115-123
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    • 2005
  • Microcapsules consisting of natural, biodegradable polymers for controlled and/or sustained core release applications are needed. Physicochemical properties of whey proteins suggest that they may be suitable wall materials in developing such microcapsules. The objectives of the research were to develop water-insoluble, whey protein-based microcapsules containing a model water-soluble drug using a chemical cross-linking agent, glutaraldehyde, and to investigate core release from these capsules at simulated physiological conditions. A model water soluble drug, theophylline, was suspended in whey protein isolate (WPI) solution. The suspension was dispersed in a mixture of dichloromethane and hexane containing 1% biomedical polyurethane. Protein matrices were cross-linked with 7.5-30 ml of glutaraldehyde-saturated toluene (GAST) for 1-3 hr. Microcapsules were harvested, washed, dried and analyzed for core retention, microstructure, and core release in enzyme-free simulated gastric fluid (SGF) and simulated intestinal fluid(SIF) at $37^{\circ}C$. A method consisting of double emulsification and heat gelation was also developed to prepare water-insoluble, whey protein-based microcapsules containing anhydrous milkfat (AMF) as a model apolar core. AMF was emulsified into WPI solution (15${\sim}$30%, pH 4.5-7.2) at a proportion of 25${\sim}$50%(w/w, on dry basis). The oil-in-water emulsion was then added and dispersed into corn oil ($50^{\circ}C$) to form an O/W/O double emulsion and then heated at $85^{\circ}C$ for 20 min for gelation of whey protein wall matrix. Effects of emulsion composition and pH on core retention, microstructure, and water-solubility of microcapsules were determined. Overall results suggest that whey proteins can be used in developing microcapsules for controlled and sustained core release applications.

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Preculture Condition for Stable Recovery of Strawberry (Fragaria × ananassa Duch.) Shoot Tips after Cryopreservation Using Droplet Vitrification

  • Lee, Young-Yi;Lee, Sun-Yi;Song, Jae-Young;Yoon, Munsup;Yi, Jung-Yoon;Lee, Jung-Ro;Kim, Haeng-Hoon
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.675-681
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    • 2020
  • This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions 'Massey' and 'MDUS3816'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 µL PVS3 on sterilized aluminum foils (4 cm× 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0mg/L GA3, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA3 for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.

On algorithm for finding primitive polynomials over GF(q) (GF(q)상의 원시다항식 생성에 관한 연구)

  • 최희봉;원동호
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.11 no.1
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    • pp.35-42
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    • 2001
  • The primitive polynomial on GF(q) is used in the area of the scrambler, the error correcting code and decode, the random generator and the cipher, etc. The algorithm that generates efficiently the primitive polynomial on GF(q) was proposed by A.D. Porto. The algorithm is a method that generates the sequence of the primitive polynomial by repeating to find another primitive polynomial with a known primitive polynomial. In this paper, we propose the algorithm that is improved in the A.D. Porto algorithm. The running rime of the A.D. Porto a1gorithm is O($\textrm{km}^2$), the running time of the improved algorithm is 0(m(m+k)). Here, k is gcd(k, $q^m$-1). When we find the primitive polynomial with m odor, it is efficient that we use the improved algorithm in the condition k, m>>1.

OGLE-2017-BLG-1049: ANOTHER GIANT PLANET MICROLENSING EVENT

  • Kim, Yun Hak;Chung, Sun-Ju;Udalski, A.;Bond, Ian A.;Jung, Youn Kil;Gould, Andrew;Albrow, Michael D.;Han, Cheongho;Hwang, Kyu-Ha;Ryu, Yoon-Hyun;Shin, In-Gu;Shvartzvald, Yossi;Yee, Jennifer C.;Zang, Weicheng;Cha, Sang-Mok;Kim, Dong-Jin;Kim, Hyoun-Woo;Kim, Seung-Lee;Lee, Chung-Uk;Lee, Dong-Joo
    • Journal of The Korean Astronomical Society
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    • v.53 no.6
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    • pp.161-168
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    • 2020
  • We report the discovery of a giant exoplanet in the microlensing event OGLE-2017-BLG-1049, with a planet-host star mass ratio of q = 9.53 ± 0.39 × 10-3 and a caustic crossing feature in Korea Microlensing Telescope Network (KMTNet) observations. The caustic crossing feature yields an angular Einstein radius of θE = 0.52 ± 0.11 mas. However, the microlens parallax is not measured because the time scale of the event, tE ≃ 29 days, is too short. Thus, we perform a Bayesian analysis to estimate physical quantities of the lens system. We find that the lens system has a star with mass Mh = 0.55+0.36-0.29 M⊙ hosting a giant planet with Mp = 5.53+3.62-2.87 MJup, at a distance of DL = 5.67+1.11-1.52 kpc. The projected star-planet separation is a⊥ = 3.92+1.10-1.32 au. This means that the planet is located beyond the snow line of the host. The relative lens-source proper motion is μrel ~ 7 mas yr-1, thus the lens and source will be separated from each other within 10 years. After this, it will be possible to measure the flux of the host star with 30 meter class telescopes and to determine its mass.

A comparative study on analgesic and non-analgesic outcomes of inter pleural analgesia compared to thoracic epidural analgesia in open pancreatico-duodenectomy

  • Lu Yao;Niroshini Rajaretnam;Natalie Smith;Lisa Massey;Somaiah Aroori
    • Annals of Hepato-Biliary-Pancreatic Surgery
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    • v.26 no.3
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    • pp.270-276
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    • 2022
  • Backgrounds/Aims: Thoracic epidural analgesia (TEA) is an established analgesic method in open Kausch-Whipple pancreaticoduodenectomy (KWPD). Although, it can cause hemodynamic instability and neurological complications. Inter pleural analgesia (IPA) is an alternative option. We aim to evaluate the effectiveness of IPA versus TEA after KWPD. Methods: We retrospectively studied the efficacy of IPA against TEA in patients, operated by a single surgeon. The primary outcome was the analgesic efficacy and secondary outcomes were analgesia-related complications, inotrope use, and duration. Results: Forty patients (TEA, 22; IPA, 18) were included. Both groups were well matched for patient characteristics, type, and duration of surgery. TEA was associated with higher analgesia-related complications (n = 8, 36.4% vs. n = 1, 5.6%; p = 0.027). TEA complications included analgesia not working (n = 4), leakage (n = 2), refractory hemodynamic instability (n = 1), and lower limb anaesthesia (n = 1). One patient in the IPA group encountered leakage. TEA was associated with longer inotrope requirement (35 vs. 18 hours; p = 0.047). There was no significant difference in intensive care unit (ITU) admission rate (81.8% vs. 77.8%; p > 0.999), median ITU stay (3 vs. 2 days, p = 0.385), or hospital stay (11 days in both groups). Conclusions: In open KWPD, IPA is not inferior to TEA in its efficacy of pain control. IPA was associated with less analgesia-related complications and shorter inotrope requirements. However, this was a small retrospective study. Larger randomized controlled trials are needed to study the effectiveness of IPA.

The Effects of Light Colors on the Behavior and Performance of Broiler Chickens (조명의 색이 육계의 행동과 생산성에 미치는 영향)

  • Son, Jang-Ho;Ravindran, Velmurugu
    • Korean Journal of Poultry Science
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    • v.36 no.4
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    • pp.329-335
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    • 2009
  • The aim of the present study was to investigate the behavior and performance of male and female broiler chickens reared under three different colors of light (white, blue and red) in a 23L:1D of lighting program. Day-old broiler chicks were obtained and assigned to six pens (16 males and 16 females per pen). Two pens each were located in three different rooms in an environmentally controlled shed and received the different light colors. All birds received same husbandry conditions. After a 3-day adaptation to the light color, behavior observations (pecking, resting, standing and walking) were made on six focal birds per pen, representing the sex ratio, by direct visual scans for 120 minutes daily for five days a week throughout the 5-week study. Body weights and feed intake were recorded at weekly intervals. On days 19 and 33, welfare indicators (tonic immobility reaction on the 19 and 33 days old; gait scores, foot pad and hock burns scores on 33 days old) were determined. Light color had no significant effect (P>0.05) on the weight gain and feed intake of broilers, but significantly (P<0.05) influenced the feed conversion ratio. Birds receiving the blue light were more (P<0.05) efficient than those receiving white and red lights. Resting was observed to be the major behavior pattern, irrespective of sex or light color. During early growth, resting behavior tended to be lower in birds receiving red light. However, standing and walking behaviors tended to be higher in birds receiving red light in 4 to 18 day-old age and all of experimental periods, respectively. These data indicate that light color can influence broiler performance and behavior.

Modification of Microclimate to Improve Milk Production in Tropical Rainforest of Thailand

  • Suriyasathaporn, W.;Boonyayatra, S.;Kreausukon, K.;Pinyopummintr, T.;Heuer, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.811-815
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    • 2006
  • The objective of this study was to evaluate the effect of electric fan installation for milk production improvement of dairy cattle in Thailand. The study was conducted using 2 small-holder dairy farms in Chiang Mai province, during April to August 2004. Electric fans were installed in front of each row of cows. Each of the two rows of cows in the barn was defined as an experimental unit, thus each farm had two experimental units. The fans were operated alternately in 7-day intervals between rows of cows within each farm during the day or between 8.00 am to 8.00 pm. Non-operation periods were used as control. Milk yields were recorded. Data on environmental temperature and humidity were obtained from Chiang Mai Meteorological Center. Result from statistically analysis of milk record suggested an interaction between lactation period and fan installation. Therefore, this interaction term of lactation period and fan installation (PERIOD_FAN) was added as a variable to the regression model. Due to the repeated data collection of milk yield from the same cow (alternate week), milk yield was analyzed by repeated measure analysis (Mixed model). Least square means were calculated for all levels and used to compare between each pair-wise values. The final data were collected from the total of 18 cows with 2,072 data. Overall means and SEM of milk yields and days in milk separated into farm were $14.7{\pm}0.06kg/day$ and $176.3{\pm}2.2days$, and $15.2{\pm}0.22kg/day$ and $202.5{\pm}3.7$ days for farm A and farm B, respectively. For multivariable analysis, only PERIOD_FAN and humidity were significantly associated with milk yield. Only the first period of lactation showed that the amount of milk yields during fan installation was higher than that of non-fan installation (p<0.05). Cows with fan installation produced approximately 1.2 kg/cow more milk than cows without fan installation during this period. In conclusion, the use of electric fan operated during the day time increased milk production of cows during the first period of lactation.