• Title/Summary/Keyword: Mass culture conditions

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A Study on the Actual Condition of Conspicuous Consumption of Clothing Mentioned in Newspaper Articles (신문기사에 나타난 복식의 과시적 소비수준분석)

  • 이민경;한명숙
    • The Research Journal of the Costume Culture
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    • v.5 no.1
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    • pp.125-135
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    • 1997
  • With rapid changes in social environments, the phenomena of consumption emerge as diverse forms in Korea-excessive consumption, impulsive consumption, imitative consumption, conspicuous consumption, obsessive consumption, etc. Especially, this researcher is interested in how conspicuous consumption of clothing has been reported in newspaper articles. Therefore, the present study is trying to infer reasons for the actual conditions of prevalent conspicuous consumption of clothing in Korean society since the 1980's. Articles in the newspaper from 1991 to 1996 have been studied to provide for study. The rapid industrialization of Korea since the 1960's has led to serious social competition and stratification. For this reason there has been a rise in the relative deprivation and alienation of low-income classes. Hence, they tend to consume clothing conspicuously in order to reduce their complex against wealthy classes. In addition, the improvement of income levels has led to a change in values towards consumption, and materialism. These changes in values along with mass communication are the main factors affecting conspicuous consumption of clothing. Finally, the present study concludes that the state of conspicuous consumption of clothing is based more on the socio-constructive dimension than the personal dimension. Further study of conspicuous consumption of clothing need to focus on the socio-constructive dimension. But because this study only infers the reasons for conspicuous consumption of clothing in terms of constructive dimension, our findings suggest further need for much more detailed and experimental discussion on the issue. The concrete reasons need to be explored in further research.

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Mass Cultivation of Rhodococcus sp. 3-2, a Carbendazim-Degrading Microorganism, and Development of Microbial Agents (카벤다짐 분해 미생물인 Rhodococcus sp. 3-2의 대량 배양 및 미생물 제제 개발)

  • Jun-Kyung Park;Seonghun Im;Jeong Won Kim;Jung-Hwan Ji;Kong-Min Kim;Haeseong Park;Yeong-Seok Yoon;Hang-Yeon Weon;Gui Hwan Han
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.259-268
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    • 2023
  • Rhodococcus sp. 3-2 strain has been reported to degrade benzimidazole-based pesticides, such as benomyl and carbendazim. Therefore, this study aimed to optimize culture medium composition and culture conditions to achieve cost-effective and efficient large-scale production of the Rhodococcus sp. 3-2 strain. The study identified that the optimal media composition for mass culture comprised 0.5% glucose, 0.5% yeast extract, 0.15% NaCl, 0.5% K2HPO4, 0.5% sodium succinate, and 0.1% MgSO4. Additionally, a microbial agent was developed using a 1.5-ton fermenter, with skim milk (20%), monosodium glutamate (15%), and vitamin C (2%) as key components. The storage stability of the microbial agent has been confirmed, with advantages of low temperature conservation, which helps to sustain efficacy for at least six months. We also assessed the benomyl degradation activity of the microbial agent within field soil. The results revealed an over 90% degradation rate when the concentration of viable cells exceeded 2.65 × 106 CFU/g after a minimum of five weeks had elapsed. Based on these findings, Rhodococcus sp. 3-2 strain can be considered a cost-effective microbial agent with diverse agricultural applications.

The Roles of Lipid Supplements in Ethanol Production Using a Continuous Immobilized and Suspended Cell Bioreactor (연속식 고정화 및 현탁 세포 생물 반응기에 의한 에탄을 생성중 지질 첨가 영향)

  • Gil, Gwang-Hoon
    • Applied Biological Chemistry
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    • v.39 no.1
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    • pp.1-8
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    • 1996
  • A one-stage, continuous-flow bioreactor with both immobilized and suspended cells was used to investigate the roles of lipid supplements in ethanol production by Saccharomyces cerevisiae. The reactor performance and the level of alcohol dehydrogenase(ADH) activities of the suspended cells, grown under various conditions, were measured. When ergosterol and/or oleic acid were added with surfactants to the yeast culture grown under non-aerated conditions, remarkable increases in ethanol production and cell growth was achieved, but specific ADH activities were not affected. Especially, no difference of specific ADH activities of the suspended cells grown under aerated and non-aerated condition was observed. The addition of the surfactant as a supplement also resulted in significant increases in ethanol production, cell growth, and specific ADH activity. When ergosterol and oleic acid were added to the yeast culture exposed to higher ethanol concentration($>40\;g/{\ell}$) level, ethanol production, cell growth, and specific ADH activity were increased, but the addition of surfactant was as effective as at lower ethanol concentration level. The results indicated that lipid supplements were more effective at higher ethanol concentration level than at lower ethanol concentration level during ethanol production. ADH isozyme patterns of the yeast cultures grown under various conditions on starch gel electrophoresis showed only one major band, probably ADH I. The migrating distance of the major isozyme, however, varied slightly according to the culture conditions of the cells. No apparent correlation was found between specific ADH activity and amount of ethanol produced. Cell mass was more important factor for ethanol production than specific ADH activity of the cells.

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Effect of Suspension Culture Conditions on Cell Activity of Wild Viola(Viola partrinii DC.) Callus (야생 흰 제비꽃(viola patrinii DC.)callus의 현탁배양 방법이 세포 활성에 미치는 영향)

  • Kim, Du-Hyun;Chung, Yong-Mo;Chung, Chung-Han;Yeeh, Yeehn;Kwon, Oh-Chang
    • Journal of Life Science
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    • v.6 no.2
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    • pp.94-103
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    • 1996
  • To understand effect of inoculum size, cell density, sucrose concentration and concentrations of MS basal on suspension culture and protoplast isolation of wild viola(Viola patrinii DC.) callus from petiole segments this experiment was conducted. In the lot of 30 mesh inoculum size, two observations were; One was that a considerable increase in the fresh and dry weight of callus was determined. Another was that the callus mass was relatively compact compared with others. A recommendable cell density was 0.4g for 20ml culture medium and the higher sucrose concentration, the higher fresh and dry weight were obtained. The dilution of MS basal salt was differently affected on fresh and dry weight; the highest fresh weight was found in 1x MS salt, while the higest dry weight was in 1/3x dilution.The addition of casein hydrolysate(3g/L) was more effective to increase of both fresh and dry weight. THe contents of protein was great in the inoculum lots with larger inoculum sizeand higher concentration of MS basal salts contenting 3g/L of casein hydrolysate and higher sucrose compared with others. The greatest protoplasts were isolated from the lot of 10 mesh size treated with 1%pectinase SE-150 and 2% cellulase YC. In general, for optimal protoplast isolation the followingconditions were recommended; 1) Use of smaller cell size cultured for 2-5 weeks, and 2) more than 5 hours incubation using the combined mixture of the enzymes with proper concentrations.

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Medium Composition Affecting In Vitro Masspropagation and Morphogenesis in Prothalli of Pteris cretica 'Wilsonii' (Pteris cretica 'Wilsonii'의 전엽체 기내 대량번식 및 형태형성에 미치는 배지 구성물질 및 배양 방법)

  • Shin, So Lim;Hwang, Ju Kwang;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.114-120
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    • 2009
  • The most effective conditions of in vitro culture were studied for mass propagation of Pteris cretica 'Wilsonii'. Spores of the species germinated within 7 weeks. The greatest proliferation was obtained with Knop and Hyponex media, but growth was more effective in Hyponex medium. MS medium induced necrosis of prothalli in all strength of nitrogen and sucrose except in case of 0% sucrose. Hyponex medium supplemented with 1% sucrose and 0.6% agar promoted propagation and growth of prothalli. In Hyponex medium, optimal inoculation method was homogenization, but in MS medium dividing colonies of prothalli was more effective. Culturing on solid medium was more effective than liquid culture method. Liquid culture induced necrosis of prothalli. Shaking cultured prothalli showed good growth, but propagation was inhibited compared to those cultured on solid medium.

Optimization of medium components and incubation time for the production of Paecilomyces tenuipes mycelia in submerged culture

  • Ha, Si Young;Jung, Ji Young;Lee, Dong Hwan;Yang, Jae-Kyung
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.1-8
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    • 2021
  • The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3-0.070169X12-0.292175X22-0.008818X32, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.

Optimum Conditions for the Culture of Hericium erinaceum in a Jar Fermenter with the Addition of Ginseng Extract in the Liquid Medium (수삼추출물을 이용한 노루궁뎅이버섯 균사체의 jar fermenter에서의 발효조건 최적화)

  • Park, Chang-Kyu;Tu, Qi;Cho, Ju-Hyun;Yu, Kwang-Won;Jeong, Heon-Sang;Lee, Hyeon-Yong;Jeong, Jae-Hyun
    • Korean Journal of Food Science and Technology
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    • v.42 no.1
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    • pp.82-89
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    • 2010
  • To obtain functional materials from a submerged culture of Hericium erinaceum, a suitable basal medium for flask culture was screened and the optimal culture conditions in a jar fermenter were investigated with the addition of ginseng extracts (GE) to the basal liquid medium. Of all tested basal liquid media, the mushroom complete medium (MCM) supplemented with 0.5% of GE produced the highest mycelial dry weight (MDW) of 5.91 g/L in the flask, which reached a plateau at $25^{\circ}C$, pH 5.5 after 10 days. The submerged culture conditions for the mass production of mycelia in a 50 L jar fermenter were also optimal at $25^{\circ}C$, pH 5.5, 120 rpm agitation speed and 0.4 vvm aeration rate. Under these conditions, the maximum MDW was produced, which reached a value of 4.28 g/L within 5 days. When we investigated the effects of the amount of GE in the MCM on the production of MDW in the jar fermenter, the addition of 5% GE (HE-GE-5) under the optimal culture conditions produced the maximum MDW (4.93 g/L). In addition, the crude polysaccharide of HE-GE-5 contained mainly neutral sugars (63.2%) with considerable amounts of uronic acid (19.3%) and a small amount of proteins (8.8%) and it had potent immunostimulation properties.

Interspecific relationship between two food organisms in the combination culture tank of rotifer, Brachionus rotundiformis and copepod, Tigriopus japonicus (로티퍼, Brachionus rotundiformis와 코페포다, Tigsiopus japonicus의 혼합 배양조에 있어서 두 종간의 상호 관계)

  • JUNG Min-Min;RHO Sum;KIM Hyeung-Sin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.66-69
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    • 2000
  • The common harpacticoida copepod species Tigriopus japnnicus was often obseued in the mass culture of marine rotifer tanks of sea farming stations. In this study, we investigated the co-existing effect of T. iaponicus to the rotifer Brachionus rotundiformis population growth. The culture conditions as temperature, salinity, culture volume, photo period, culture period and observation interval were 25{\circ}C, 22ppt, 40ml, 12L:12D, 16 deys$ and every two days during the experimental period, respectively. Nannochloropsis oculata was used as the food for the two testing organisms. After counting the growth of rotifer and copepod, they were transferred to new culture tank with N. oculata at the density of $7{times}10^5\;cells/ml$. The population growth of B. rotundiformis was suppressed by T. japonicus when these two organisms were mix-cultured comparing to single culture of B. rotundiformis. But population growth of T, japomcus was not affected by the presence of B. rotundiformis. The interspecific relationships of prey-predation and food competition were not observed in the combination culture of rotifer B. rotundiformis and copepod T japonicus.

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주정증류 폐액을 이용한 Actinobacillus sp. EL-9로부터 Poly-$\beta$-Hydroxybutyrate의 생산 및 폐약의 처리

  • 손홍주;이상준
    • Microbiology and Biotechnology Letters
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    • v.24 no.3
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    • pp.352-356
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    • 1996
  • Alcoholic distillery wastes are utilized as dual purposes to produce PHB in lower production cost and to reduce the amount of waste to be treated. In this study, various attempts were made to increase PHB production under various conditions by Actinobacillus sp. EL-9 in a shaker culture. The addition of glucose, NH$_{4}$NO$_{3}$ to alcoholic distillery wastes slightly promoted cell mass and PHB production. Enzyme hydrolysis of alcoholic distillery wastes increased the production of PHB than that of untreated waste and acid hydrolysis treatment. The PHB weight in alcoholic distillery wastes was 1.91 g/l. Fermentation process of PHB production reduced the amount of COD value up to 54%, which reduced organic loading rate and capacity of activated sludge system.

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Penicillium sp.-L4의 균성장 및 효소작용을 억제하는 $\beta$-glucosidase inhibitor의 분리 및 특성

  • Kim, Moo-Sung;Ha, Sung-Yoon;Jeon, Gi-Boong;Lim, Dal-Taek;Park, Byung-Hwa;Lee, Bo-Seop;Lee, Sang-Rin;Choi, Yong-Keel
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.189-196
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    • 1997
  • A producer of inhibitor against ${\beta}-glucosidase$ of Penicillium sp.-L4 was screened from Actinomycetes, and the isolated strain was identified as Streptomyces sp. The inhibitor produced was very stable against heat, acidic and alkaline conditions, proteolytic and amylolytic enzymes. The inhibotor was purified from culture broth through activated carbon treatment, ultrafiltration, anion and cation exchange, activated carbon columm, acetone precipitation and preparative HPLC. It showed inhibitory activities against a variety of dissacharide hydrolyzing enzymes produced by P.sp.-L4, and the mode of inhibition was competitive. Its structure and molecular formular was elucidated by IR, $^1H\;and\;^{13}C$ NMR and FAB/Mass spectrometry, which was identified as 1-deoxynojirimycin (dNM). dNM showed inhibitory effects on the cell growth and hydrolytic enzyme action of P.sp.-L4 on agar plate and infected lemon peel.

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